scholarly journals Deacetylation Followed by Fractionation of Yellow Poplar Sawdust for the Production of Toxicity-Reduced Hemicellulosic Sugar for Ethanol Fermentation

2018 ◽  
Author(s):  
Seong Ju Kim ◽  
Tae Hyun Kim ◽  
Kyeong Keun Oh
Keyword(s):  
Ethanol Production ◽  
Ethanol Yield ◽  
Alkali Treatment ◽  
Pichia Stipitis ◽  
Ammonia Solution ◽  
Dilute Acid ◽  
Yellow Poplar ◽  
Theoretical Ethanol Yield ◽  
Optimized Conditions ◽  
Poplar Sawdust

In order to produce bioethanol from yellow poplar sawdust without detoxification, deacetylation (mild alkali treatment) was performed with aqueous ammonia solution. To select the optimal conditions, deacetylation process was carried out using different conditions: NH4OH loading (2–10% (w/v)) and solid-to-liquid ratio (1:4–10) at 121 °C for 60 min. In order to assess the effectiveness of deacetylation, fractionation of deacetylated yellow poplar sawdust was performed using dilute acid (H2SO4, 0.5–2.0% (w/v)), reaction temperature (130–150 °C) and time (10–80 min). The toxicity-reduced hemicellulosic hydrolysates that were obtained through a two-step treatment at optimized conditions were fermented using Pichia stipitis for ethanol production, without any further detoxification. The maximum ethanol production was 4.84 g/L, corresponding to a theoretical ethanol yield of 82.52%, which is comparable to those of intentionally made hydrolyzates as controls.

scholarly journals Ethanol Production from Nondetoxified Dilute-Acid Lignocellulosic Hydrolysate by Cocultures of Saccharomyces cerevisiae Y5 and Pichia stipitis CBS6054

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ping Wan ◽  
Dongmei Zhai ◽  
Zhen Wang ◽  
Xiushan Yang ◽  
Shen Tian
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Synthetic Medium ◽  
Pichia Stipitis ◽  
Dilute Acid ◽  
Acid Hydrolysate ◽  
Issatchenkia Orientalis ◽  
Final Ethanol Concentration

Saccharomyces cerevisiae Y5 (CGMCC no. 2660) and Issatchenkia orientalis Y4 (CGMCC no. 2159) were combined individually with Pichia stipitis CBS6054 to establish the cocultures of Y5 + CBS6054 and Y4 + CBS6054. The coculture Y5 + CBS6054 effectively metabolized furfural and HMF and converted xylose and glucose mixture to ethanol with ethanol concentration of 16.6 g/L and ethanol yield of 0.46 g ethanol/g sugar, corresponding to 91.2% of the maximal theoretical value in synthetic medium. Accordingly, the nondetoxified dilute-acid hydrolysate was used to produce ethanol by co-culture Y5 + CBS6054. The co-culture consumed glucose along with furfural and HMF completely in 12 h, and all xylose within 96 h, resulting in a final ethanol concentration of 27.4 g/L and ethanol yield of 0.43 g ethanol/g sugar, corresponding to 85.1% of the maximal theoretical value. The results indicated that the co-culture of Y5 + CBS6054 was a satisfying combination for ethanol production from non-detoxified dilute-acid lignocellulosic hydrolysates. This co-culture showed a promising prospect for industrial application.

Bioconversion of De-Oiled Rice Bran (DORB) Hemicellulosic Hydrolysate into Ethanol by Pichia stipitis NCM3499 under Optimized Conditions

2009 ◽  
Vol 5 (1) ◽  
Author(s):  
Anuj Kumar Chandel ◽  
Mangamoori Lakshmi Narasu ◽  
Ravinder Rudravaram ◽  
Ravindra Pogaku ◽  
Linga Venkateswar Rao
Keyword(s):  
Ethanol Production ◽  
Rice Bran ◽  
Process Parameters ◽  
Reducing Sugars ◽  
Carbon Sources ◽  
Pichia Stipitis ◽  
Medium Temperature ◽  
Carbon Substrates ◽  
Total Reducing Sugars ◽  
Optimized Conditions

In the present paper, parametric optimization studies were performed to determine the most influential range of process parameters for maximum ethanol production from Pichia stipitis NCIM3499 by one-at-a-time method under submerged fermentation conditions. Various parameters, such as agitation, pH of medium, temperature and different carbon sources, etc., were taken into consideration. The optimum values of these process parameters were as follows: agitation (200 rpm), pH (5.5), temperature (30°C), and semi-aerobic conditions (150 ml fermentation media in 250 ml Erlenmeyer flask). Among the different carbon substrates tested, glucose and xylose (20±0.21 g/l) showed same ethanol production (8.8±0.35 g/l) with a yield of 0.44±0.11g/g sugar utilized. These process parameters were applied for the ethanol production from de-oiled rice bran (DORB), the left over residue after extraction of oil. It was thermochemically saccharified using sulfuric acid (0.5-5.5% v/v) at 120°C for a fixed residence time of 1h. A maximum of 38.50±0.45 g/l total reducing sugars was obtained at 3.5% v/v H2SO4. This sugar syrup was detoxified by overliming with calcium hydroxide and subsequently fermented with P. stipitis NCIM3499 under the optimized conditions. After 72 h of incubation, DORB hydrolysate (33.50±0.44g/l total reducing sugars) showed maximum ethanol production (12.47±0.26g/l) with the yield 0.42±0.021g/g and fermentation efficiency of 81.74±0.55%. To the best of our knowledge, this is the first report on ethanol production from de-oiled rice bran using P. stipitis NCIM3499.

A Comparison of Aqueous and Dilute-Acid Single-Temperature Pretreatment of Yellow Poplar Sawdust

2001 ◽  
Vol 40 (10) ◽  
pp. 2352-2361 ◽  
Author(s):  
Stephen Glen Allen ◽  
Deborah Schulman ◽  
Joseph Lichwa ◽  
Michael Jerry Antal ◽  
Edward Jennings ◽  
...  
Keyword(s):  
Dilute Acid ◽  
Yellow Poplar ◽  
Single Temperature ◽  
Temperature Pretreatment ◽  
Poplar Sawdust

Enhanced ethanol production from deacetylated yellow poplar acid hydrolysate by Pichia stipitis

2010 ◽  
Vol 101 (13) ◽  
pp. 4947-4951 ◽  
Author(s):  
Dae Haeng Cho ◽  
Soo-Jeong Shin ◽  
Yangwon Bae ◽  
Chulhwan Park ◽  
Yong Hwan Kim
Keyword(s):  
Ethanol Production ◽  
Pichia Stipitis ◽  
Yellow Poplar ◽  
Acid Hydrolysate

scholarly journals Potential of Candida glabrata from ragi as a bioethanol producer using selected carbohydrate substrates

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Micky Vincent ◽  
Queentety Johnny ◽  
Dayang Salwani Awang Adeni ◽  
Nurashikin Suhaili
Keyword(s):  
Ethanol Production ◽  
Candida Glabrata ◽  
Ethanol Yield ◽  
Fermented Food ◽  
Ethanol Conversion ◽  
Ethanolic Fermentation ◽  
Different Types ◽  
Notable Increase ◽  
Theoretical Ethanol Yield ◽  
Simultaneous Saccharification

Abstract. Vincent M, Johnny Q, Adeni DSA, Suhaili N. 2020. Potential of Candida glabrata from ragi as a bioethanol producer using selected carbohydrate substrates. Nusantara Bioscience 12: 1-10. The flexibility and efficiency of fermenting microorganisms to convert substrates to ethanol are important factors in achieving high bioethanol yields during ethanolic fermentation. In this study, Candida glabrata, a common yeast found in fermented food, was evaluated in terms of its capability to produce ethanol using different types of carbohydrates, which included simple saccharides (glucose, maltose, sucrose), polysaccharides (starch and cellulose) and complex carbohydrates (total sago effluent, TSE). Our results indicated that C. glabrata was able to efficiently produce ethanol from glucose at 79.84% TEY (Theoretical Ethanol Yield). The ethanol production from sucrose was low, which was only 6.44% TEY, while no ethanol was produced from maltose. Meanwhile, for complex carbohydrate substrates such as starch and cellulose, ethanol was produced only when supplementary enzymes were introduced. Simultaneous Saccharification and Fermentation (SSF) of starch dosed with amylases resulted in an ethanol yield of 55.08% TEY, whilst SSF of cellulose dosed with cellulases yielded a TEY of 31.41%. When SSF was performed on TSE dosed with amylases and cellulases, the highest ethanol production was recorded within 24 h, with a yield of 23.36% TEY. Lactic acid and acetic acid were found to be at minimal levels throughout the fermentation period, indicating an efficient ethanol conversion. A notable increase in C. glabrata biomass was observed in cultures fed with glucose, starch (with supplementary amylases), and TSE (with supplementary amylases and cellulases). The current study indicates that C. glabrata can be used for bioethanol production from glucose, polysaccharides, and complex starchy lignocellulosic substrates such as TSE via SSF.

scholarly journals Bioconversion of Municipal Solid Wastes for Bioethanol Production

2017 ◽  
Vol 14 (3) ◽  
pp. 1151-1157 ◽  
Author(s):  
J. Patra ◽  
A. Basu ◽  
A. Mishra ◽  
N. K. Dhal
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Acid Treatment ◽  
Ethanol Yield ◽  
Environmental Issues ◽  
Pichia Stipitis ◽  
Solid Wastes ◽  
Dilute Acid ◽  
Municipal Solid Wastes ◽  
Time Point

ABSTRACT: The use of dilute acid (H2SO4, 3%) and alkali (NaOH, 3%) pretreatment methods has some potential how ever to date, these methods effectively increase ethanol production of municipal solid waste (MSW). Enzymatic hydrolysis was carried out with Aspergillus niger, Aspergillus fumigatus and Trichoderma reesei. Finally, the fermentation was done by sugar three ethanologenic yeasts, Saccharomyces cerevisiae, pichia stipitis, canida shehatae for bioethanol production.The highest ethanol yield (22.32%) v/v. was obtained with a pre-hydrolysis treatment consisting of NaOH at 3% concentration, followed by Pichia stipitis and enzymatic hydrolysis with Aspergillus niger. Pre-hydrolysis treatment consisted Enzymatic hydrolysis was carried out with Alkali pretreated wastes yield more sugar as compared to acid treatment using produced more ethanol than others at each time point. The experimental results observed that 80% of the cellulose converted to glucose from the waste which can be easily fermented to production. of ethanol. The ability focus on related environmental issues, such as sustainable waste management, climate change, land use and biodiversity, are discussed.

scholarly journals Ethanol production by Pichia stipitis immobilized on sugarcane bagasse

Bioethanol ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
BS Yñiguez-Balderas ◽  
B Ortiz-Muñiz ◽  
J Gómez-Rodríguez ◽  
B Gutierrez-Rivera ◽  
MG Aguilar-Uscanga
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Ethanol Yield ◽  
Raw Materials ◽  
Pichia Stipitis ◽  
Inoculum Size ◽  
Process Efficiency ◽  
Immobilized Cell ◽  
Production Technologies ◽  
Gab Model

AbstractThe search for new ethanol production technologies is due to this biofuel being a renewable and environmentally friendly option. Immobilized cell systems for ethanol production have been studied; however, the phenomenon involved in cell sorption on raw materials has been poorly explored. Therefore, this work evaluates P. stipitis immobilization on sugarcane bagasse pretreated with sulphuric acid, as well as ethanol production in batch culture. The results obtained showed that the Guggenheim-Anderson-de Boer (GAB) model explained the sorption phenomenon. The selected inoculum size for immobilization was the same as the monolayer sorption capability (1.17 gl-1). Using 1:100 g ml- 1 solid-liquid ratio, at 250 rpm, ethanol yield and productivity of 0.404 gg-1 glucose and 0.41 gl-1h-1 were obtained, respectively. The immobilized systems were stable for up to twenty-five repeated batches (36 h each). Ethanol production was increased from the first to the twenty-fifth batch (18.1 and 24.7 gl-1 ethanol). The use of complex media, such as molasses “B” or sugarcane hydrolyzates, caused an increase in process efficiency 2.4 and 1.8-fold respectively, compared with free cells systems. Biotechnological ethanol production from lignocellulosic hydrolyzates could be improved by the use of the immobilization cell sorption on pre-treated raw materials.

scholarly journals A Hydrotrope Pretreatment for Stabilized Lignin Extraction and High Titer Ethanol Production

2022 ◽  
Author(s):  
Hairui Ji ◽  
Le Wang ◽  
Furong Tao ◽  
Zhipeng Yao ◽  
Xuezhi Li ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Ethanol Yield ◽  
High Titer ◽  
Enzymatic Saccharification ◽  
Value Added ◽  
Enzymatic Digestion ◽  
Lignin Extraction ◽  
C 30 ◽  
Theoretical Ethanol Yield ◽  
Light Color

Abstract The biomass pretreatment strategies using organic acids facilitate lignin removal and enhance the enzymatic digestion of cellulose. However, lignin always suffers a severe and irreversible condensation. The newly generated C-C bonds dramatically affect its further upgrading. In this study, we used a recyclable hydrotrope (p-Toluenessulfonic acid, p-TsOH) to dissolve lignin under mild condition and stabilized lignin with a quenching agent (formaldehyde, FA) during extraction, achieving both value-added lignin extraction and efficient enzymatic saccharification of cellulose. Approximately 63.7% of lignin was dissolved by 80% (wt. %) p-TsOH with 1.5% FA addition at 80 o C, 30 min. The obtained lignin was characterized by FTIR spectroscopy, TGA, 2D HSQC NMR spectroscopy, and GPC. The results indicated that the extracted lignin exhibited excellent properties, such as light color, a low molecular weight (Mw, 5371 g/mol), and a narrow polydispersity (Mw/Mn, 1.63). The pretreated substrate was converted to ethanol via a quasi-simultaneous saccharification and fermentation process (Q-SSF). After fermentation of 60 h, the ethanol concentration reached 38.7±3.3 g/L which was equivalent to a theoretical ethanol yield of 82.9±2.2% based on the glucan content, while the residual glucose concentration was only 4.69±1.4 g/L. In short, this pretreatment strategy protected lignin to form new C-C linkages and improved the enzymatic saccharification of glucan for high-titer ethanol production.

Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells of Pichia stipitis NCIM 3498

2011 ◽  
Vol 9 (1) ◽  
Author(s):  
Chandrasekhar Gajula ◽  
Anuj Kumar Chandel ◽  
Radhika Konakalla ◽  
Ravinder Rudravaram ◽  
Ravindra Pogaku ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Immobilized Cells ◽  
Pichia Stipitis ◽  
Fuel Ethanol ◽  
Enzymatic Hydrolysate ◽  
Groundnut Shell

scholarly journals Enzymatic Process for Cystoseira barbata Valorization: Ethanol Production and Additional By-Products

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 741
Author(s):  
Doinita-Roxana Cioroiu Tirpan ◽  
Ancaelena Eliza Sterpu ◽  
Claudia Irina Koncsag ◽  
Alina Georgiana Ciufu ◽  
Tănase Dobre
Keyword(s):  
Ethanol Production ◽  
Alcoholic Fermentation ◽  
Ethanol Yield ◽  
Product Yield ◽  
Liquid Ratio ◽  
Experimental Conditions ◽  
Factors Affecting ◽  
Cystoseira Barbata ◽  
Main Factors ◽  
Process Factors

The aim of this study is to evaluate the potential of dried Cystoseira barbata alga for ethanol production through alcoholic fermentation. The influence of the main factors affecting the fermentation are studied in the frame of a 23 factorial experimental plan. The main factors influencing the process are the fermentation temperature (t from 25 °C to 35 °C), the solid to liquid ratio (S/L from 0.040 g/g to 0.080 g/g), and the cellulase ratio (R from 8 U/g d.m to 16 U/g d.m.). The maximum volatile compounds yield of 0.2808 g/g d.m and ethanol yield of 0.0158 g/g d.m were favored by the following experimental conditions: process temperature of 35 °C, solid to liquid ratio of 0.0415, and enzyme ratio of 16 U/g d.m. A statistical model was used to correlate the product yield with the process factors. Additionally, 19 interesting bioactive compounds were found in the enzymatic hydrolysis and alcoholic fermentation broths which seem likely to maintain natural defence mechanisms against diseases and physical disorders.

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