scholarly journals The Relationship between Acrosome Reaction and Polyunsaturated Fatty Acid Composition in Boar Sperm

2019 ◽  
Author(s):  
Sang-Hee Lee ◽  
Yu-Jin Kim ◽  
Byeong Ho Kang ◽  
Choon-Keun Park
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Acid Composition ◽  
Acrosome Reaction ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Boar Sperm ◽  
The Relationship

This study investigated the relationship of acrosome reactions and fatty acid composition on fertility in boar sperm. The acrosome reaction of sperm was induced via methyl-beta-cyclodextrin (MBCD), and acrosome reaction, plasma membrane integrity, and fertility were analyzed. The fatty acid composition of the excess acrosome reacted sperm was determined via gas chromatography. The results showed that the acrosome reaction in sperm was induced over 85% of the time by 60 mM MBCD treatment, and the plasma membrane integrity was significantly decreased and was dependent on the MBCD level. The acrosome reacted sperm resulted in significantly higher saturated fatty acids (SFAs) and lower unsaturated fatty acids (PUFAs) than the non-acrosome reaction group. Moreover, the acrosome reacted sperm from 60 mM MBCD significantly decreased in vitro fertility and blastocyst formation relative to non-acrosome reacted sperm, and the acrosome reaction was positively correlated with SFAs and negatively correlated with PUFAs. Of these fatty acids, C22:5n-6 (docosapentaenoic acid [DPA]) and C22:6n-3 (docosahexaenoic acid [DHA]) were directly negatively correlated with the acrosome reaction (r = -0.982 and -0.947, respectively). In conclusion, the excessive acrosome reactions may occur by reducing the PUFAs, which may then dramatically decrease sperm fertility in pigs.

The relationship between acrosome reaction and polyunsaturated fatty acid composition in boar sperm

2020 ◽  
Vol 55 (5) ◽  
pp. 624-631
Author(s):  
Sang‐Hee Lee ◽  
Yu‐Jin Kim ◽  
Byeong Ho Kang ◽  
Yong Sik Yun ◽  
Choon‐Keun Park
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Polyunsaturated Fatty Acid ◽  
Acrosome Reaction ◽  
Boar Sperm ◽  
The Relationship

scholarly journals The Relationship Between Fatty Acid Composition and Cold Hardiness of Pecan Cultivars

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 858A-858
Author(s):  
Sameera Bafeel* ◽  
Frank Matta
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Cold Acclimation ◽  
Acid Composition ◽  
Cold Hardiness ◽  
Unsaturated Fatty Acids ◽  
Time Period ◽  
Cultivated Plant ◽  
The Relationship

Temperature is a major environmental factor governing the distribution of both wild and cultivated plant species. During acclimation and deacclimation plants undergo a series of metabolic changes that lead to cold hardiness or loss of hardiness. One of these changes is the accumulation of certain lipids. This research was conducted to compare hardiness among three pecan cultivars: `Desirable', `Jackson', and `Owens' growing under Mississippi condition and to determine the relationship between fatty acid levels and cold hardiness of pecan shoots. Differential thermal analysis (DTA), electrical conductivity, and tetrazolium tests were used to determine cold hardiness. Pecan stems were collected from September to March in 2002 and 2003 to determine cold acclimation and deacclimation. Fatty acid composition of pecan stems during this time period was determined by gas chromatography. DTA indicated that pecan stems acclimated in October and deacclimated in March. During cold acclimation, there was a shift in the fatty acid composition to more unsaturated fatty acids. The percentage of linoleic and linolenic fatty acids increased, while the percentage of palmitic and stearic fatty acids decreased. The correlation between unsaturated fatty acids and cold hardiness suggests that unsaturated fatty acid may play a role in membrane fluidity.

scholarly journals Can the early stage copepad (Copepodites and Naupliies) abundance play important role on the fatty acid composition of Sagitta setosa (Chaetognatha) in the Southeastern Black Sea?

2020 ◽  
Vol 37 (4) ◽  
pp. 335-342
Author(s):  
Nurgül Sen Özdemir ◽  
Ali Muzaffer Feyzioglu ◽  
Fatma Caf
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Early Stage ◽  
Sampling Period ◽  
Copepod Abundance ◽  
Parallel Increase ◽  
The Relationship ◽  
Parallel Distribution

In this study, the relationship between the fatty acid composition, abundance of carnivore Sagitta setosa (S. setosa) and total copepod abundance (copepodites and nauplii) which are the main diets of S. setosa was investigated. Sampling was conducted monthly during a year. During the sampling period, S. setosa and the total the copepod abundance showed a parallel distribution until November. Average total lipid was at the highest with 3% in spring and autumn, while the lowest was 2% in winter and summer. While DHA/EPA ratio (2.23) as carnivory index in S. setosa and the total copepod (copepodites and nauplii) abundance (1375 ind/m3) were at the highest in winter, but another carnivory index, 18:1ω9/18:1ω7 ratio did not show a parallel increase with copepod abundance. However, 18:1ω9 which increases the carnivory index, is among the main fatty acids in S. setosa. This might be indicated that S. setosa does not related to predation on the copepods, but it shows that feeding in a carnivorous style. Additionally, the herbivory index ∑ n-3/∑ n-6 ratio was detected in spring and autumn at the highest level. Consequently, although there is no direct effect between the copepod abundance and S. setosa fatty acids, it is possible an indirect effect.

scholarly journals Lipidomic markers of sperm cryotolerance in cattle

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Holly C. Evans ◽  
Thu T. N. Dinh ◽  
Muhammet Rasit Ugur ◽  
Mustafa Hitit ◽  
Dishnu Sajeev ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Polar Lipid ◽  
Assisted Reproductive Technologies ◽  
Saturated Fatty Acids ◽  
Lipid Fraction ◽  
Membrane Integrity ◽  
Reproductive Technologies

AbstractThe objective of the current study was to determine the fatty acid composition of sperm from Holstein bulls with different freezability (Good and Poor; n = 12). Fatty acids were extracted from frozen sperm in 1:2 (v/v) chloroform–methanol solvent, fractionated into neutral and polar fractions, and composition determined by gas chromatography–mass spectrometry. Thirty-four fatty acids were quantified and their concentrations and percentages within each lipid fraction were calculated. Overall, saturated fatty acids (SFA) were predominant, accounting for 71 to 80% of fatty acids in neutral and polar lipid factions. There were marked differences in fatty acid composition between the lipid fractions (P < 0.001). The branched chain fatty acid (BCFA) concentration (15 to 18 µg) was almost twice as much as polyunsaturated fatty acids (PUFA) concentration found in the polar lipid fraction (8 to 9 µg; P < 0.001). Sperm with different freezability phenotypes only had a few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (P ≤ 0.011). These results are significant because they reveal key understandings of fatty acid composition of sperm membrane and lay a foundation for the manipulation of membrane integrity, fluidity, and stability to advance the assisted reproductive technologies.

scholarly journals Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm

Reproduction ◽  
2006 ◽  
Vol 131 (5) ◽  
pp. 887-894 ◽  
Author(s):  
K E Waterhouse ◽  
P O Hofmo ◽  
A Tverdal ◽  
R R Miller
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Survival Rate ◽  
Acid Composition ◽  
Plasma Membranes ◽  
Female Reproductive Tract ◽  
Freezing And Thawing ◽  
Acrosome Integrity

The response of sperm to cryopreservation and the fertility of frozen–thawed semen varies between species. Besides species differences in sperm physiology, structure and biochemistry, factors such as sperm transport and female reproductive tract anatomy will affect fertility of frozen–thawed semen. Therefore, studying differences in sperm cryotolerance between breeds and individuals instead of between species may reveal sources of variability in sperm cryotolerance. In the present study, the effect of cooling, re-warming and freezing and thawing on plasma membrane and acrosome integrity of sperm within and between Norwegian Landrace and Duroc breeds was studied. Furthermore, the relation between post-thaw survival rate and fatty acid composition of the sperm plasma membranes was investigated. Flow cytometry assessments of plasma membrane and acrosome integrity revealed no significant differences between breeds; however there were significant male-to-male variations within breeds in post-thaw percentages of live sperm (plasma membrane intact). The most abundant fatty acids in the plasma membranes from both breeds were palmitic acid (16:0), stearic acid (18:0), oleic acid (18:1, n-9), docosapentaenoic acid (22:5, n-6) and docosahexaenoic acid (22:6, n-3). The ratio of ∑ 22:5, n-6 and 22:6, n-3/∑ all other membrane fatty acids was significantly related to survival rate (plasma membrane integrity) of sperm for both Norwegian Landrace (correlation coefficient (rs) = 0.64,P< 0.05) and Duroc (rs= 0.67,P< 0.05) boars. In conclusion, male-to-male differences in sperm survival rate after freezing and thawing may be partly related to the amount of long-chain polyunsaturated fatty acids in the sperm plasma membranes.

scholarly journals Relationship between the content of chlorinated hydrocarbons and fatty acid composition of milk fat

2018 ◽  
Vol 62 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Renata Pietrzak-Fiećko
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Negative Correlation ◽  
Milk Fat ◽  
Environmental Pollutants ◽  
Cow Milk ◽  
Positive Correlation ◽  
The Relationship

AbstractIntroductionReports that the presence of persistent organic pollutants in fat may affect fatty acid metabolism prompted this research aiming to study the relationship between the contents of γ-HCH and DDT, DDE, DDD, and ΣDDT, and fatty acid composition of milk fat.Material and MethodsThe material consisted of 50 samples of cow and mare milk, collected in 2015. Ludwicki’s and the Röse-Gottlieb and IDF Standard methods were used to prepare the samples. Statistical analyses were conducted using Statistica 12.0.ResultsThere was a negative correlation between the content of γ-HCH and C16:1, C17:1, C18:1c9, C18:1c9c12, and ΣMUFA in cow milk fat and C13:0, C14:0, and C10:1 in mare milk fat. A positive correlation was observed between γ-HCH and C6:0 to C12:0, C14:0, C18:1t16, and ΣSFA in cow milk fat, and between this compound and C14:0iso, C16:1, C17:1, C18:1c9,11, and ΣMUFA in mare milk fat. A negative correlation between the contents of ΣDDT and C16:1, C17:1, C18:1c9,11,13 and ΣMUFA in cow milk fat and C16:0iso, C17:0, and C18:3 in mare milk fat was noted. A positive correlation was found between the contents of ΣDDT and saturated and polyunsaturated fatty acids and ΣSFA and ΣPUFA in cow milk fat, and C18:2c9c12 in mare milk fat.ConclusionThe correlation between the content of selected organochlorine compounds and the composition of fatty acids in cow and mare milk fat indicates the strong influence of these environmental pollutants on the nutritional value of milk fat.

scholarly journals Erhebungen zur Fettsäurenzusammensetzung von Rückenfett bei Thüringer Schweinen sowie zum Fett von Thüringer Knackwürsten aus dem Einzelhandel

2003 ◽  
Vol 46 (3) ◽  
pp. 257-267
Author(s):  
W. Reichardt ◽  
E. Gernand ◽  
S. Müller ◽  
H. Hartung ◽  
B. Eckert ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Linolenic Acid ◽  
Acid Composition ◽  
Retail Trade ◽  
Relationship Of ◽  
The Relationship

Abstract. Title of the paper: Examinations to the fatty acid composition of backfat at Thuringian pigs as well as to the fat of Thuringian cracking sausages from the retail trade The fat from the outer back bacon of Thuringian pigs (n = 751) was examined several years with regard to their fatty acid composition. The content of polyunsaturated fatty acids (PUFA) ranged at the backfat from 7.3 to 12.7% and the relationship of the n-6 and n-3 fatty acids varied from 4.4 to 11.3. PUFA contents in the backfat > 12 % were explained by high C18:2 or C18:3 contents of the food. Examination of the fatty acid composition of the fat of 21 fresh cracking sausages from the Thuringian retail trade on a random basis yielded, that the contents of PUFA and linolenic acid ranged from 6.2 to 15.8 % respectively from 0.8 to 2.0 % as well as the quotient of n-6 to n-3 fatty acids varied from 3.6 to 10.3.

Sign in / Sign up

Export Citation Format

Share Document