scholarly journals Stem Cell Surgery and Growth Factors in Retinitis Pigmentosa Patients: Pilot Study After Literature Review

2019 ◽  
Author(s):  
Paolo Giuseppe Limoli ◽  
Enzo Maria Vingolo ◽  
Marco Ulises Morales ◽  
Celeste Limoli ◽  
Marcella Nebbioso
Keyword(s):  
Growth Factors ◽  
Retinitis Pigmentosa ◽  
Platelet Rich Plasma ◽  
Functional Results ◽  
Point Of View ◽  
Foveal Thickness ◽  
Restoration Technique ◽  
Number Of Patients ◽  
Group A ◽  
Group B

To evaluate whether autologous mesenchymal cells, adipose derived stem cells and platelet-rich plasma, grafted into the supracoroideal space by surgical treatment according to Limoli retinal restoration technique (LRRT), can produce growth factors in order to exert a beneficial effect in retinitis pigmentosa (RP) patients. Twenty-one eyes underwent surgery and divided based on retinal foveal thickness ≤ 190 or >190 µm into group A and group B, respectively. The specific LRRT triad was grafted in a deep scleral pocket above the choroid of each eye. At 6-month follow-up, group B showed an improvement in residual close-up visus and sensitivity at microperimetry compared to group A. After an in-depth review of molecular biology studies concerning degenerative phenomena underlying the etiopathogenesis of RP, it can be confirmed that further research is needed on tapeto-retinal degenerations both from a clinical and molecular point of view to obtain better functional results. In particular, it is necessary to increase the number of patients, extend observation times, and treat subjects in the presence of still trophic retinal tissue to allow adequate biochemical and functional catering.

scholarly journals Stem Cell Surgery and Growth Factors in Retinitis Pigmentosa Patients: Pilot Study after Literature Review

Biomedicines ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 94 ◽  
Author(s):  
Paolo Giuseppe Limoli ◽  
Enzo Maria Vingolo ◽  
Celeste Limoli ◽  
Marcella Nebbioso
Keyword(s):  
Retinitis Pigmentosa ◽  
Platelet Rich Plasma ◽  
Functional Results ◽  
Point Of View ◽  
Foveal Thickness ◽  
Restoration Technique ◽  
Number Of Patients ◽  
Group A ◽  
Cell Surgery ◽  
Group B

To evaluate whether grafting of autologous mesenchymal cells, adipose-derived stem cells, and platelet-rich plasma into the supracoroideal space by surgical treatment with the Limoli retinal restoration technique (LRRT) can exert a beneficial effect in retinitis pigmentosa (RP) patients. Twenty-one eyes underwent surgery and were divided based on retinal foveal thickness (FT) ≤ 190 or > 190 µm into group A-FT and group B-FT, respectively. The specific LRRT triad was grafted in a deep scleral pocket above the choroid of each eye. At 6-month follow-up, group B showed a non-significant improvement in residual close-up visus and sensitivity at microperimetry compared to group A. After an in-depth review of molecular biology studies concerning degenerative phenomena underlying the etiopathogenesis of retinitis pigmentosa (RP), it was concluded that further research is needed on tapeto-retinal degenerations, both from a clinical and molecular point of view, to obtain better functional results. In particular, it is necessary to increase the number of patients, extend observation timeframes, and treat subjects in the presence of still trophic retinal tissue to allow adequate biochemical and functional catering.

scholarly journals Two or four injections of platelet-rich plasma for osteoarthritic knee did not change synovial biomarkers but similarly improved clinical outcomes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Srihatach Ngarmukos ◽  
Chotetawan Tanavalee ◽  
Chavarin Amarase ◽  
Suphattra Phakham ◽  
Warayapa Mingsiritham ◽  
...  
Keyword(s):  
Growth Factors ◽  
Synovial Fluid ◽  
Clinical Outcomes ◽  
Inflammatory Cytokines ◽  
Platelet Rich Plasma ◽  
Short Form ◽  
Patient Reported ◽  
Group A ◽  
Baseline Levels ◽  
Group B

AbstractWe compared two and four intra-articular injections of platelet-rich plasma (PRP) in terms of changes of synovial cytokines and clinical outcomes. One hundred twenty-five patients having knee osteoarthritis (OA) underwent PRP injections at a 6-week interval. Before each PRP injection, synovial fluid aspiration was collected for investigation. Patients were divided into two or four intra-articular PRP injections (group A and B, respectively). Changes in synovial biomarkers were compared with the baseline levels of both groups, and clinical outcomes were evaluated until one year. Ninety-four patients who had completed synovial fluid collection were included for final evaluation, 51 in group A and 43 in group B. There were no differences in mean age, gender, body mass index (BMI), and radiographic OA grading. The average platelet count and white blood cell count in PRP were 430,000/µL and 200/ µL, respectively. There were no changes of synovial inflammatory cytokines (IL-1β, IL-6, IA-17A, and TNF-alpha), anti-inflammatory cytokines (IL-4, IL-10, IL-13, and IL-1RA), and growth factors (TGF-B1, VEGF, PDGF-AA, and PDGF-BB) between baseline levels and six weeks in group A, and 18 weeks in group B. Both groups had significantly improved clinical outcomes from six weeks including visual analog scale (VAS), patient-reported outcome measures [PROMs; Western Ontario and McMaster Universities Osteoarthritis (WOMAC) Index and Short Form-12 (SF-12)], with a significant delayed improvement of performance-based measures [PBMs; time up and go (TUG), 5-time sit to stand test (5 × SST), and 3-min walk test (3-min WT)]. In conclusion, two- or four-PRP intra-articular injection at a 6-week interval for knee OA demonstrated no changes of synovial cytokines and growth factors but similarly improved clinical outcomes from 6 weeks until 1 year.

scholarly journals Comparative study of efficacy of platelet rich plasma injection versus corticosteroid injection in conservative management of periarthritis shoulder

2021 ◽  
Vol 11 (2) ◽  
pp. 274-279
Author(s):  
Sambit Kumar Panda ◽  
Surai Soren ◽  
Ashok Kumar Nayak ◽  
Rabindra Nayak ◽  
Sabyasachi Swain
Keyword(s):  
Range Of Motion ◽  
Platelet Rich Plasma ◽  
Corticosteroid Injection ◽  
Diabetes Patient ◽  
Chi Square ◽  
Home Based ◽  
Number Of Patients ◽  
Group A ◽  
Group B ◽  
Quick Dash

Analysis of eighty patients with periarthritis shoulder was done. Patients were thoroughly evaluated and were divided into two groups in a randomized trial. Forty patients were in group-A who received 3doses of injection of PRP (4ml) 2 weeks apart within a duration of 6weeks. Equal number of patients were in group-B. They received 2ml of Injection corticosteroid 2weeks apart within a duration of 6 weeks. All participants were advised to perform a home-based hot fomentation and 15min exercise therapy. One participant from group A and 2 from group B were lost to follow up. There were 35 male and 42 female who completed the study. Analysis of 77 subjects who completed the study was done. Participant were evaluated for range of motion of shoulder as main outcome measure. Visual Analogue Scale (VAS) and (Quick DASH) was used to measure pain and functions of the shoulder. The evaluation of Participants was done at 0,3,6 and 12 weeks. ANOVA test and Chi-square test, was repeatedly used to measure the differences.Participants who were given PRP injections showed significant improvements in active and passive range of shoulder motion as measured by VAS and Quick DASH over corticosteroid injection. This was also reflected statistically. No major adverse reactions were observed during 12 weeks of intervention.In our study, the injection of PRP showed marked improvement in the range of motion of shoulder over corticosteroid injection but it needs other study to be treatment of choice. It emerged as an option for treatment in diabetes patient and condition where steroid is contraindicated.

scholarly journals Laser for post acne scars with or without growth factors: a comparative clinical trial

2020 ◽  
Vol 6 (2) ◽  
pp. 224
Author(s):  
Lakkireddygari Sujana ◽  
Balachandra S. Ankad ◽  
Savitha L. Beergouder
Keyword(s):  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Objective Assessment ◽  
Subjective Assessment ◽  
Primary Objective ◽  
Treatment Modalities ◽  
Acne Scars ◽  
New Strategy ◽  
Group A ◽  
Group B

<p class="abstract"><strong>Background:</strong> Treatment of acne scars presents a considerable challenge to dermatologists. But with advent of fractional CO<sub>2</sub> (FCO<sub>2</sub>) laser and platelet-rich plasma (PRP), new avenues can be explored. PRP provides various growth factors, so that the healing of laser-damaged skin is accelerated. The aim of this study was to assess the efficacy and safety of FCO<sub>2</sub> laser alone and in combination with PRP in patients with post-acne scars.</p><p class="abstract"><strong>Methods:</strong> Eighty patients with acne scars were counseled and randomly assigned into two groups of 40 each. Group A was treated with FCO<sub>2</sub> laser alone, while Group B was treated with FCO<sub>2</sub> laser and PRP for 6 months. Primary objective was assessment of efficacy of both treatment modalities. Secondary objective was evaluation of safety of treatments.<strong></strong></p><p class="abstract"><strong>Results:</strong> At the end of 6 months, group A showed about 18% reduction, while group B showed about 33% reduction in acne scars on objective assessment. Patient satisfaction as assessed by subjective assessment was greater in group B than in group A. Duration of erythema, edema and crusting was lesser in group B compared to group A.</p><p class="abstract"><strong>Conclusions:</strong> Combination of FCO<sub>2</sub> and PRP was found to be more efficacious as compared to FCO<sub>2</sub> laser alone. Combining the treatment modalities can provide a new strategy for treating the post-acne scars.</p>

scholarly journals Two or four injections of leukocyte-poor platelet-rich plasma for osteoarthritic knee did not affect changes of synovial cytokines and growth factors but similarly improved clinical outcomes

2021 ◽  
Author(s):  
Srihatach Ngarmukos ◽  
Chotetawan Tanavalee ◽  
Chavarin Amarase ◽  
Suphattra Phakham ◽  
Warayapa Mingsiritham ◽  
...  
Keyword(s):  
Growth Factors ◽  
Synovial Fluid ◽  
Clinical Outcomes ◽  
Inflammatory Cytokines ◽  
Platelet Rich Plasma ◽  
Knee Oa ◽  
Group A ◽  
Baseline Levels ◽  
One Year ◽  
Group B

Abstract We compared two and four intra-articular injections of platelet-rich plasma (PRP) in terms of changes of synovial cytokines and clinical outcomes. One hundred twenty-five patients having knee OA underwent leukocyte-poor PRP with a 6-week interval. Before each PRP injection, synovial fluid aspiration was collected for investigation. Patients were divided into two or four intra-articular PRP injections (group A and B, respectively). Changes in synovial cytokines and growth factors were compared with the baseline levels of both groups, and clinical outcomes were evaluated until one year. Ninety-four patients who had completed synovial fluid collection were included for final evaluation, 51 in group A and 43 in group B. There were no differences in mean age, gender, BMI, and OA grading. The average platelet count in PRP was 430,000/µL. There were no changes of synovial inflammatory cytokines (IL-1ß, IL-6, IA-17A, and TNF-alpha), anti-inflammatory cytokines (IL-4, IL-10, IL-13, and IL-1RA), and growth factors (TGF-B1, VEGF, PDGF-AA, and PDGF-BB) between baseline levels and six weeks in group A, and 18 weeks in group B. Both groups had significantly improved clinical outcomes from six weeks including visual analog scale (VAS), patient-reported outcome measure (PROM; WOMAC and SF-12), with a significant delayed improvement of performance-based measure [PBM; time up and go (TUG), 5-time sit to stand test (5×SST), and 3-minute walk test (3-min WT)]. In conclusion, two- or four-PRP injection at a 6-week interval for knee OA demonstrated no changes of synovial cytokines and growth factors but similarly improved clinical outcomes from 6 weeks until one year.

Clinical Correlation between Retinal Sensitivity and Foveal Thickness in Retinitis Pigmentosa Patients

2016 ◽  
Vol 27 (3) ◽  
pp. 352-356 ◽  
Author(s):  
Enzo M. Vingolo ◽  
Vittoria De Rosa ◽  
Erika Rigoni
Keyword(s):  
Linear Regression ◽  
Retinitis Pigmentosa ◽  
Healthy Subjects ◽  
Retinal Sensitivity ◽  
Foveal Thickness ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Sd Oct

Purpose To investigate the relationship between retinal structure and retinal sensitivity comparing data obtained using spectral-domain optical coherence tomography (SD-OCT) and microperimetry in retinitis pigmentosa (RP) and healthy patients. Methods A total of 49 patients (98 eyes) with RP were divided into 4 groups according to the OCT findings: group A, patients with no macular changes; group B, patients with cystoid macular edema (CME); group C, patients with vitreomacular traction (VMT); group D, patients with retinal thinning. As a control group, we enrolled 27 healthy subjects. All subjects underwent a complete ophthalmologic examination, including SD-OCT and microperimetry. For the statistical analysis, Pearson correlation test and linear regression analysis were used. A p value <0.05 was considered statistically significant. Results We did not find any significant difference in best-corrected visual acuity (BCVA), foveal thickness, or retinal sensitivity between group A and the healthy subjects (p>0.05). In group B, linear regression of logMAR versus foveal thickness and retinal sensitivity versus foveal thickness gave r values of 0.931 and 0.786, respectively. In group C, r values were 0.786 and 0.842, respectively, while in group D they were 0.816 and 0.795. Conclusions This article shows the first application of the new microperimeter MP3 in patients with RP, which has proven to be very sensitive in detecting functional abnormalities in all the patients. Future studies should investigate the relationships among photoreceptor cell loss, retinal sensitivity, and fixation in patients with RP.

scholarly journals COMPARATIVE STUDY OF PLATELET-RICH PLASMA AND CORTICOSTEROID INJECTION IN THE TREATMENT OF PLANTAR FASCIITIS

2019 ◽  
Vol 3 (11) ◽  
Author(s):  
Dr. S. A. Mustafa Johar ◽  
Dr. Rajeev Parmar ◽  
Dr. Tushaar Ghodawat
Keyword(s):  
Clinical Evaluation ◽  
Plantar Fasciitis ◽  
Platelet Rich Plasma ◽  
Corticosteroid Injection ◽  
Functional Results ◽  
Blood Component ◽  
Maximum Point ◽  
Group A ◽  
Autologous Platelet Rich Plasma ◽  
Group B

Background: Plantar fasciitis is defined as localized inflammation due to chronic repeated microinjury to the substance of plantar aponeuroses and the patients present to the clinic for pain in heel. In this study, autologous platelet-rich plasma (PRP), a concentrated bioactive blood component rich in  growth factors, was compared to traditional steroid injection usually methylprednisolone  in the treatment of plantar fasciitis resistant to traditional nonoperative management. Methods: Eighty patients with plantar fasciitis were included in the study and randomly categorised into group A (steroid inj) and Group B (prp inj). 3 cc PRP or 40 mg DepoMedrol injected into plantar aponeurosis at maximum point of tenderness, in both groups separately. Results of both groups comparedand Score of both the groups tellied by using vas score. Study Design and Time: Prospective cohort study of 6 months duration. Inclusion criteria: All cases of plantar fasciitis except cases of metabolic disorder Exclusion criteria: other plantar pathologies and deformities. calcaneum fractures Old treated with injections. Results: The instillation of PRP found to be more effective than Steroids injection in terms of pain and functional results in the treatment of plantar fasciitis. Clinical evaluation was performed before treatment and at the 6th week, 12th week and 24th weeks from instillation of PRP/steroid in follow up visits. Visual analog scale were used in the clinical evaluation. Keywords: Platelet-Rich, Plasma, Corticosteroid, Plantar & Fasciitis. 

scholarly journals Outcome of staged injection of autologous platelet rich plasma in treatment of mild to moderate knee osteoarthritis

2019 ◽  
Vol 6 (1) ◽  
pp. 26
Author(s):  
Rajat Gupta ◽  
Ram Avtar ◽  
Krishan Kumar
Keyword(s):  
Growth Factors ◽  
Single Injection ◽  
Platelet Rich Plasma ◽  
Low Cost ◽  
Biologically Active ◽  
Plasma Injection ◽  
Group A ◽  
Autologous Platelet Rich Plasma ◽  
Group B ◽  
Autologous Platelet

<p class="abstract"><strong>Background:</strong> Autologous platelet-rich plasma (PRP) offers an easy solution for delivering multiple growth factors needed for tissue repair. Intra-articular injections of PRP have been proposed as a simple low cost minimally invasive way to obtain the concentration of growth factors and biologically active molecules to promote cartilage healing in osteoarthritic (OA) knee joint. The objective of the present study was designed to<em> </em>evaluate the clinical efficacy of autologous platelet rich plasma injection in mild to moderate osteoarthritic knee and to assess the role of serial staged autologous platelet rich plasma injection.</p><p class="abstract"><strong>Methods:</strong> 50 patients with mild to moderate osteoarthritis of knee were divided into two groups. Group A was given staged injection of freshly prepared autologous PRP in the affected knee. Group B was given single injection of PRP. The Results were evaluated on the basis of the Western Ontario and McMaster Universities Arthritis Index (WOMAC) questionnaire and visual analog scale (VAS) before treatment and 3 weeks, 8 weeks, 16 weeks and 24 weeks after treatment.<strong></strong></p><p class="abstract"><strong>Results:</strong> At 24-weeks follow up the following observations were made; the MeanVAS in Group A decreased to 3.30 whereas in Group B it increased to 4.54. The Mean WOMAC score in Group A was reduced to a mere28.64 in Group A and in Group B it was 39.76.</p><p class="abstract"><strong>Conclusions:</strong> It is concluded from our study that concomitant use of serial staged injection (two) of PRP over four months is more effective than single injection in patients with mild to moderate OA of knee.</p>

scholarly journals Platelet-Rich Plasma Injection in Patients with Atrophic Rhinitis

ORL ◽  
2021 ◽  
pp. 1-8
Author(s):  
Do Hyun Kim ◽  
Min Hyeong Lee ◽  
Jaeyoon Lee ◽  
Eun A. Song ◽  
Soo Whan Kim ◽  
...  
Keyword(s):  
Platelet Rich Plasma ◽  
Inferior Turbinate ◽  
Nasal Symptom ◽  
Atrophic Rhinitis ◽  
Symptom Improvement ◽  
Plasma Injection ◽  
Group A ◽  
Group B ◽  
Contrast Group ◽  
Snot 22

<b><i>Objective:</i></b> To investigate the effect of platelet-rich plasma (PRP) injection in patients with atrophic rhinitis. <b><i>Methods:</i></b> Prepared PRP was injected into the inferior turbinate bilaterally, and nasal bacterial cultures were conducted. Improvement of symptoms was assessed with the Nasal Obstruction Symptom Evaluation (NOSE) and the Sino-Nasal Outcome Test-22 (SNOT-22). Nasal mucociliary clearance was assessed using the saccharin transit time (STT). <b><i>Results:</i></b> In the PRP-injected group (group A), NOSE (throughout the study) and SNOT-22 (1 month after injection) scores were significantly decreased during the study. However, the saline spray group (group B) showed no significant nasal symptom improvement during the study period. In group A, the STT was improved until 3 months after the injection. In contrast, group B showed STT improvement after 2 months that was maintained throughout the study. <b><i>Conclusion:</i></b> PRP injections can improve nasal symptoms and nasal mucociliary function in patients with atrophic rhinitis.

scholarly journals INTRAVENOUS REGIONAL ANESTHESIA (BIERS BLOCK);

2012 ◽  
Vol 19 (05) ◽  
pp. 710-714
Author(s):  
Muhammad ASGHER ◽  
ASIM GHAURI ◽  
MUHAMMAD ABDULLAH ◽  
Tariq Abassi
Keyword(s):  
Regional Anaesthesia ◽  
Military Hospital ◽  
P Value ◽  
Complete Evaluation ◽  
Intravenous Regional Anesthesia ◽  
Number Of Patients ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
Intravenous Regional Anaesthesia

Objective: To compare the analgesic effects of combination of 0.5% Lidocaine plus Ketorolac in intravenous regionalanaesthesia technique with those of Lidocaine (0.5%) alone to prevent post operative pain after intravenous regional anaesthesia (Biersblock). Study design: Randomized Control Trial. Place and duration of study: The study was carried out at Department of Anaesthesiology,Intensive Care and pain management, Combined Military hospital, Rawalpindi from July 2008 to February 2009. Patients and Methods: Thestudy was conducted after complete evaluation of risk / benefit ratio to the patients. On the basis of random number method the patients weredivided into two equal groups (group A and group B). The number of patients in each group was 75. Group A was assigned Lidocaine in a dose of200mg 40ml of 0.5% solution and group B was assigned injection Ketorolac 30mg added to Lidocaine in a dose of 200mg 40ml of 0.5% solution.The patients were kept in post anaesthesia care unit for two hours and pain intensity was measured by visual analogue scale(VAS) on 15,30minutes,1hour, 1.5 and at 2 hours after the cuff deflation. The analgesic efficacy recorded on the basis of visual analog scale of two groups, wascompared using student’s t - test. p value of less than 0.05 was considered statistically significant. Results: In group A 33 males and 42 femaleswere enrolled for the study while in group B there were 38 males and 37 females. The mean age of the patients in group A was 34.31 ± 6.03years while in group B was 32.99 ± 6.08 years. Patients were also classified according to ASA classification in which 87 patients were classifiedas ASA – I and 63 patients as ASA – II. Group B which received Ketorolac in addition to Lidocaine for Bier’s block had low visual analogue scoresas compared to group A which received only Lidocaine for Bier’s block. P values obtained after the comparison of the mean VAS of two groupsat 15 minutes, 30 minutes, 1 hour, 1.5 hours and 2 hours were all less than 0.05 (0.002 for 15 minutes, 0.004 for 30 minutes, 0.001 for 1 hour,0.004 for 1.5 hours and 0.001 for 2 hours). Conclusions: Ketorolac improves the postoperative analgesia markedly when used with Lidocainein intravenous regional anaesthesia.

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