Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

2019 ◽  
Author(s):  
Kentaro Yoshida ◽  
Yu Kashimura ◽  
Toshio Kamijo ◽  
Tetsuya Ono ◽  
Takenori Dairaku ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Dna Cleavage ◽  
Electrostatic Interactions ◽  
Glucose Solution ◽  
Layer By Layer ◽  
Sensitive Layer ◽  
Physiological Conditions ◽  
Lbl Films

Glucose-sensitive films were prepared by the layer-by-layer (LbL) deposition of poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx)). H-PEI/DNA+GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA+GOx)5 film was then partially decomposed by hydrogen peroxide (H2O2). The mechanism for the decomposition of the LbL film was considered to involve a more reactive oxygen species (ROS) that was formed by the reaction of hemin and H2O2, which then caused nonspecific DNA cleavage. GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the H-PEI/DNA+GOx)5 film was observed when the thin film was immersed in a glucose solution. A (H-PEI/DNA+GOx)5 film exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated decomposition of the film increased with the time. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strength close to physiological conditions, it was possible to slowly decompose the LbL film at a sub-millimolar glucose concentration.

scholarly journals Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

Polymers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 319 ◽  
Author(s):  
Kentaro Yoshida ◽  
Yu Kashimura ◽  
Toshio Kamijo ◽  
Tetsuya Ono ◽  
Takenori Dairaku ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Dna Cleavage ◽  
Electrostatic Interactions ◽  
Glucose Solution ◽  
Layer By Layer ◽  
Sensitive Layer ◽  
Physiological Conditions ◽  
Lbl Films ◽  
Low Glucose

Glucose-sensitive films were prepared through the layer-by-layer (LbL) deposition of hemin-modified poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx)). H-PEI/DNA + GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA + GOx)5 film was then partially decomposed by hydrogen peroxide (H2O2). The mechanism for the decomposition of the LbL film was considered to involve more reactive oxygen species (ROS) that were formed by the reaction of hemin and H2O2, which then caused nonspecific DNA cleavage. In addition, GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the (H-PEI/DNA + GOx)5 film was observed when the thin film was immersed in a glucose solution. (H-PEI/DNA + GOx)5 films exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated that the decomposition of the film increased with the time to 9.97%, 16.3%, 23.1%, and 30.5%, respectively. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strengths close to physiological conditions, it was possible to slowly decompose the LbL film at low glucose concentrations of 1–10 mM.

scholarly journals Glucose biosensor based on entrapment of glucose oxidase and myoglobin in silica gel by the sol-gel method

2005 ◽  
Vol 19 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Mohammed A. Zaitoun
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Decay Curve ◽  
Sol Gel ◽  
Porous Silica ◽  
Glucose Biosensor ◽  
Negative Peak ◽  
Before And After ◽  
Porous Silica Glass

A spectrophotometric method is presented to determine glucose employing the sol-gel technique. Myoglobin (Mb) and glucose oxidase are encapsulated in a transparent and porous silica glass. The produced gel (xerogel) is then immersed in water where increments of glucose are added to the solution with stirring; glucose diffuses into the sol-gel glass pores and a series of reactions take place. Glucose is first oxidized by glucose oxidase and oxygen to gluconate and hydrogen peroxide is generated. The liberated hydrogen peroxide oxidizes the Mb heme (Fe2+into Fe3+). The higher is the glucose concentration added, the more is the H2O2generated, and the more is the Mb oxidation (Fe2+to Fe3+) and as a result the higher is the absorbance at 400 nm (negative peak, lower absorbance value). All measurements are performed at this wavelength (400 nm), the negative peak obtained by subtracting the absorption spectra of Mb before and after oxidation. Measuring the slope of the absorbance decay versus time at 400 nm monitors increments of added glucose. Each glucose concentration has an accompanying unique decay curve with a unique slope. The higher is the glucose concentration; the steeper is the decay curve (higher slope value). The calibration curve was linear up to 40 mM.

Glucose-induced decomposition of layer-by-layer films composed of phenylboronic acid-bearing poly(allylamine) and poly(vinyl alcohol) under physiological conditions

2015 ◽  
Vol 3 (39) ◽  
pp. 7796-7802 ◽  
Author(s):  
Katsuhiko Sato ◽  
Mao Takahashi ◽  
Megumi Ito ◽  
Eiichi Abe ◽  
Jun-Ichi Anzai
Keyword(s):  
Glucose Oxidase ◽  
Vinyl Alcohol ◽  
Phenylboronic Acid ◽  
Poly Vinyl Alcohol ◽  
Layer By Layer ◽  
Physiological Conditions ◽  
Induced Decomposition

Phenylboronic acid-bearing poly(allylamine)/poly(vinyl alcohol) layer-by-layer films coupled with glucose oxidase decomposed in the presence of glucose under physiological conditions.

scholarly journals Preparation of Microparticles Capable of Glucose-Induced Insulin Release under Physiological Conditions

Polymers ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1164 ◽  
Author(s):  
Kentaro Yoshida ◽  
Kazuma Awaji ◽  
Seira Shimizu ◽  
Miku Iwasaki ◽  
Yuki Oide ◽  
...  
Keyword(s):  
Boronic Acid ◽  
Oxidation Reaction ◽  
Shikimic Acid ◽  
Multilayer Films ◽  
Electrostatic Repulsion ◽  
Layer By Layer ◽  
Sensitive Layer ◽  
Boronate Ester ◽  
Physiological Conditions ◽  
Phenyl Boronic Acid

Hydrogen peroxide (H2O2)-sensitive layer-by-layer films were prepared based on combining phenyl boronic acid (PBA)-modified poly(allylamine) (PAH) with shikimic acid (SA)-modified-PAH through boronate ester bonds. These PBA-PAH/SA-PAH multilayer films could be prepared in aqueous solutions at pH 7.4 and 9.0 in the presence of NaCl. It is believed that the electrostatic repulsion between the SA-PAH and PBA-PAH was diminished and the formation of ester bonds between the SA and PBA was promoted in the presence of NaCl. These films readily decomposed in the presence of H2O2 because the boronate ester bonds were cleaved by an oxidation reaction. In addition, SA-PAH/PBA-PAH multilayer films combined with glucose oxidase (GOx) were decomposed in the presence of glucose because GOx catalyzes the oxidation of D-glucose to generate H2O2. The surfaces of CaCO3 microparticles were coated with PAH/GOx/(SA-PAH/PBA-PAH)5 films that absorbed insulin. A 1 mg quantity of these particles released up to 10 μg insulin in the presence 10 mM glucose under physiological conditions.

Plasma glucose measurement with the Yellow Springs Glucose Analyzer.

1978 ◽  
Vol 24 (1) ◽  
pp. 150-152 ◽  
Author(s):  
K S Chua ◽  
I K Tan
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Plasma Glucose ◽  
Coefficient Of Variation ◽  
Glucose Concentration ◽  
Quantitative Measurement ◽  
Reaction Chamber ◽  
Glucose Measurement ◽  
Immobilized Glucose Oxidase ◽  
Carry Over

Abstract The Yellow Springs Glucose Analyzer, a device for the quantitative measurement of glucose concentrations, involves the use of immobilized glucose oxidase, incorporated on a membrane covering a hydrogen peroxide sensor. Operation of the instrument is simple. After an initial calibration, 25 microliter of plasma is injected into a reaction chamber. At 45 s a digital result for glucose is displayed. The within-batch coefficient of variation for the method is 1.2% or less for glucose concentrations of 0.94 to 3.98 g/liter. The between-batch coefficient of variation is 5.8% or less for glucose concentrations of 0.29 to 2.91 g/liter. Concentration and readout are linearly related to at least 4.6 g/liter. Analytical recoveries ranged from 100 to 102%. Carry-over was negligible. Values for glucose concentration obtained with the instrument compared well (r = 0.997) with those obtained with the Beckman Glucose Analyzer.

Collaborative Study of Enzymatic Glucose Determination in Corn Starch Hydrolysates

1969 ◽  
Vol 52 (3) ◽  
pp. 556-559
Author(s):  
J T Brady ◽  
J A Zagorski
Keyword(s):  
Hydrogen Peroxide ◽  
Gas Chromatography ◽  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Corn Starch ◽  
Collaborative Study ◽  
Color Intensity ◽  
Glucose Determination ◽  
Colored Product ◽  
Oxidation Of Glucose

Abstract Glucose in starch hydrolysates was determined by catalytic oxidation of glucose with glucose oxidase to form hydrogen peroxide which, in the presence of peroxidase and a chromogen, yields a colored product. This product, when acidified, is very stable and the color intensity is proportional to the glucose concentration in the sample. Fermco Test S.F.G., a package containing all the ingredients necessary for the reaction, was used. Eleven collaborators who analyzed five different sirup samples found the method to be adequate for determining glucose. Results compared well with those by paper and gas chromatography. The method is recommended for adoption as official first action.

Glucose in Serum and Cerebrospinal Fluid by Direct Application of a Glucose Oxidase Method

1968 ◽  
Vol 14 (6) ◽  
pp. 548-554 ◽  
Author(s):  
Arnold G Ware ◽  
Edward P Marbach
Keyword(s):  
Hydrogen Peroxide ◽  
Cerebrospinal Fluid ◽  
Glucose Oxidase ◽  
Coefficient Of Variation ◽  
Glucose Concentration ◽  
Reduction Method ◽  
Molecular Iodine ◽  
Normal Serum ◽  
Direct Application

Abstract Glucose is measured directly in 0.02 ml. of serum or cerebrospinal fluid by reaction with glucose oxidase. The hydrogen peroxide produced reacts with iodide in the presence of a catalyst to form molecular iodine. The iodine color is proportional to the glucose and is measured photometrically. The reaction is carried out directly on serum following preincubation with molecular iodine and can be completed in 15 min. The results on normal serum average 8 mg./100 ml. lower than those obtained with the AutoAnalyzer reduction method. At a glucose concentration of 150 mg./100 ml., the procedure has a coefficient of variation of 1.4%. Recovery of added glucose averaged 98%. Hemolysis, lipemia, or icterus do not interfere.

Selective Hydrogen Bonding Controls Temperature Response of Layer-by-Layer Upper Critical Solution Temperature Micellar Assemblies

Soft Matter ◽  
2021 ◽  
Author(s):  
Aliaksei Aliakseyeu ◽  
Victoria Albright ◽  
Danielle Yarbrough ◽  
Samantha Hernandez ◽  
Qing Zhou ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Methacrylic Acid ◽  
Temperature Response ◽  
Solution Temperature ◽  
Layer By Layer ◽  
Critical Solution Temperature ◽  
Hydrogen Bonding Interactions ◽  
Upper Critical Solution Temperature ◽  
Lbl Films

This work establishes a correlation between the selectivity of hydrogen-bonding interactions and the functionality of micelle-containing layer-by-layer (LbL) assemblies. Specifically, we explore LbL films formed by assembly of poly(methacrylic acid)...

scholarly journals Thermally Stimulated Desorption Optical Fiber-Based Interrogation System: An Analysis of Graphene Oxide Layers’ Stability

Photonics ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 70
Author(s):  
Maria Raposo ◽  
Carlota Xavier ◽  
Catarina Monteiro ◽  
Susana Silva ◽  
Orlando Frazão ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Optical Fiber ◽  
Optical Fiber Sensor ◽  
Layer By Layer ◽  
Film Stability ◽  
A Value ◽  
Device Reliability ◽  
Sensor Devices ◽  
Lbl Films ◽  
The Stability

Thin graphene oxide (GO) film layers are being widely used as sensing layers in different types of electrical and optical sensor devices. GO layers are particularly popular because of their tuned interface reflectivity. The stability of GO layers is fundamental for sensor device reliability, particularly in complex aqueous environments such as wastewater. In this work, the stability of GO layers in layer-by-layer (LbL) films of polyethyleneimine (PEI) and GO was investigated. The results led to the following conclusions: PEI/GO films grow linearly with the number of bilayers as long as the adsorption time is kept constant; the adsorption kinetics of a GO layer follow the behavior of the adsorption of polyelectrolytes; and the interaction associated with the growth of these films is of the ionic type since the desorption activation energy has a value of 119 ± 17 kJ/mol. Therefore, it is possible to conclude that PEI/GO films are suitable for application in optical fiber sensor devices; most importantly, an optical fiber-based interrogation setup can easily be adapted to investigate in situ desorption via a thermally stimulated process. In addition, it is possible to draw inferences about film stability in solution in a fast, reliable way when compared with the traditional ones.

scholarly journals Cultivation of Saccharomyces cerevisiae with Feedback Regulation of Glucose Concentration Controlled by Optical Fiber Glucose Sensor

Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 565
Author(s):  
Lucie Koštejnová ◽  
Jakub Ondráček ◽  
Petra Majerová ◽  
Martin Koštejn ◽  
Gabriela Kuncová ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Optical Fiber ◽  
Glucose Concentration ◽  
Glucose Sensor ◽  
Ruthenium Complex ◽  
Feedback Regulation ◽  
Regulation System ◽  
Sensitive Layer ◽  
Average Deviation ◽  
Glucose Content

Glucose belongs among the most important substances in both physiology and industry. Current food and biotechnology praxis emphasizes its on-line continuous monitoring and regulation. These provoke increasing demand for systems, which enable fast detection and regulation of deviations from desired glucose concentration. We demonstrated control of glucose concentration by feedback regulation equipped with in situ optical fiber glucose sensor. The sensitive layer of the sensor comprises oxygen-dependent ruthenium complex and preimmobilized glucose oxidase both entrapped in organic–inorganic polymer ORMOCER®. The sensor was placed in the laboratory bioreactor (volume 5 L) to demonstrate both regulations: the control of low levels of glucose concentrations (0.4 and 0.1 mM) and maintenance of the glucose concentration (between 2 and 3.5 mM) during stationary phase of cultivation of Saccharomyces cerevisiae. Response times did not exceed 6 min (average 4 min) with average deviation of 4%. Due to these regulation characteristics together with durable and long-lasting (≥2 month) sensitive layer, this feedback regulation system might find applications in various biotechnological processes such as production of low glucose content beverages.

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