scholarly journals Clonal Propagation of Flacourtia indica for Ensuring Quality Planting Materials and Sustainable Supply of Edible Fruits

2017 ◽  
Vol 5 (1) ◽  
pp. 42
Author(s):  
Md. Alamgir Kabir ◽  
Md. Hasan Mehedi Khan ◽  
Md. Masudur Rahman ◽  
A.T.M. Rabiul Alam ◽  
S.M. Hemayet Jahan ◽  
...  

The present study was carried out at the Agriculture research field, Patuakhali Science And Technology University(PSTU), Patuakhali, from March, 2015 to April, 2016 to explore the domestication potential and to evaluate the rooting performance of Flacourtia indica (katabohori), a wild fruit species in Bangladesh, through clonal propagation by stem cutting under 3 different doses of rooting hormone IBA (Indole Buetaric Acid) and planted in the perforated plastic tray filled with coarse sand and gravel placed in the non-mist propagator. The experiment was laid out following a Randomized Complete Block Design (RCBD) with 4 treatments and 4 replications (blocks). The treatments were T0= control, T1 = 0.2% IBA, T2 = 0.4% IBA, T3 = 0.8% IBA. The rooting ability of cuttings was significantly influenced by the application of IBA. The results showed that the highest rooting percentage (100) and maximum root number (8) of Flacourtia indica stem cuttings were obtained from the cuttings treated with 0.4% IBA followed by 0.2% IBA where as the longest root length (8.998 cm) was recorded with 0.2% IBA followed by 0.4% IBA. Survival percentage of the cutlings (the rooted cuttings) after 3 months of transferring them into poly bags was also significantly enhanced by exogenous rooting hormone (IBA) application. The highest survival percentage (84.5 %) was for the cuttings treated with 0.4% IBA followed by 0.2% IBA (71%). The similar trend was also observed for average cutling height and number of leaf. Findings of the present study reveal that the plant species is highly amenable for clonal propagation by stem cuttings using low-cost non-mist propagator. Considering both rooting percentage and root number, 0.4% IBA treatment of stem cuttings may be recommended for mass production of quality planting stocks for the domestication of the species in homestead agroforestry or in fruit orchards to provide edible fruit to rural poor people of natural disaster vulnerable Bangladesh.

2017 ◽  
Vol 5 (2) ◽  
pp. 185
Author(s):  
Md. Alamgir Kabir ◽  
Mahamud Hasan Prince ◽  
Rezaul Karim ◽  
Saifur Rahman ◽  
K.M. Masum Billah ◽  
...  

The present study was carried out to explore rooting performance of Punica granatum (Dalim) in Bangladesh through clonal propagation by stem cutting under 3 different doses of rooting hormone IBA (Indole Buetairic Acid) and planted in the perforated plastic tray filled with coarse sand and gravel placed in the non-mist propagator. The experiment was laid out following a Randomized Complete Block Design (RCBD) with 4 treatments and 4 replications (blocks). The treatments were T0= control, T1 = 0.2% IBA, T2 = 0.4% IBA, T3 = 0.8% IBA. The rooting ability of cuttings was significantly influenced by the application of IBA. The results showed that the highest rooting percentage (70%) and longest root length (11.75 cm) of P. granatum stem cuttings were obtained from the cuttings treated with 0.2% IBA followed by 0.4% IBA (47.5% and 7.95 cm respectively) whereas the highest root number (32) and maximum root diameter (2.7 mm) were recorded with 0.4% IBA followed by 0.8% IBA (28 and 2.47 mm respectively). Survival percentage of the cutlings (the rooted cuttings) after 3 months of transferring them into poly bags was also significantly enhanced by exogenous rooting hormone (IBA) application. The highest survival percentage (73.5%) was observed with 0.4% IBA treatment followed by 0.8% IBA (68.5%). Findings of the present study reveal that P. granatum is highly amenable for clonal propagation by stem cuttings using low-cost non-mist propagator and 0.4% IBA treatment may be recommended for mass production of quality planting stocks for the cultivation of the species in homestead agroforestry or in fruit orchards. These outcomes can assist to provide edible fruit to poor rural people of natural disaster vulnerable Bangladesh.


2017 ◽  
Vol 5 (2) ◽  
pp. 193
Author(s):  
Hassan Najman Muhamed ◽  
Nasser Abdusalam Dawod

Because of the difficulty in their vegetative propagation, both Rhus coriaria L. and Cotinus coggygria Scop.  (Ancardaceae) are rarely introduced into the forest nursery trade. Vegetative propagation is practiced in forestry to produce seedlings of desired genetic properties within a short period and when seeds displaying a status of complex dormancy. In the present study, the possibility of the multiplication by stem cuttings was studied for Sumac, the high ethnobotanical shrub value and for Cotinus, the endangered shrub, to give more insight into their propagation technique. Between January-July 2014, using complete randomized block design (C.R.B.D) in four replicates, we evaluated the effectiveness of applying four different Indole-3-Butyric Acid (IBA) concentration to two stems cutting diameters in producing C. coggygria and R. coriaria. However, the former species due to the poor survival percentage (lower 5%) excluded from the analysis. Optimum results of C. coggygria were obtained with IBA 20000 mg.l -1 and cutting diameter more than 1 cm. (rooting % 60, number of roots 17.33, number of branches  3.75 and the number of leaves 19.33 while nonsignificant increase was recorded in root length. 16.33 cm.). The results indicated that Cotinus is relatively easy to propagate by stem cutting in comparing with Sumac with rootability increases significantly with increasing of IBA concentration and stem cutting diameter. Further studies are needed for more reasonable rooting results to meet the large quantity demand of elite seedling material that could be later used to restore the natural populations of C. coggygria and R. coriaria.


2000 ◽  
Vol 10 (4) ◽  
pp. 780-784
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart

The effects of 1H-indole-3-butyric acid (IBA), cutting position on stock plants, the date of propagation, the type of rooting substrate and temperature on rooting of mussaenda (Mussaenda erythrophylla Schumach. & Thonn. `Ashanti Blood' and `Rosea', and Mussaenda philippica A. Rich `Aurorae') stem cuttings were determined. Cuttings of `Ashanti Blood' produced the largest number of roots when treated with 15 mmol (3000 ppm) IBA and rooted in perlite at 29 °C (84 °F). Cutting position on stock plants did not affect rooting in any of the three cultivars. Propagation date and temperature of the rooting medium affected root numbers in `Aurorae'. With `Rosea', only the type of rooting substrate affected root number. Rooting percentage was 22%, 48%, and 39% in `Ashanti Blood', `Aurorae', and `Rosea' respectively. After 30 days of propagation average root length was 4, 12, and 4 mm (0.2, 0.5, and 0.2 inch) in `Ashanti Blood', `Aurorae', and `Rosea' respectively. Growers must determine precise rooting conditions for each cultivar to obtain consistent rooting of cuttings. This process may not be economically feasible on a commercial basis because rooting percentages are relatively low. We conclude that other methods of clonal propagation need to be evaluated before uniform rooted stem cuttings of mussaenda can be produced economically.


Author(s):  
Ibrar Hussain ◽  
Abdul Samad ◽  
Nisar Naeem ◽  
Ziaullah ◽  
Hafeez ur Rehman ◽  
...  

Fig is a perennial fruit tree with high economic importance among horticultural crops in some countries. The fruit has a good market value, both at national and international level. The objective of this work was to evaluate the potential of vegetative propagation of fig cvs. ‘Sawari’ and ‘Tarnab Inzar’ through stem cuttings with different lengths, aiming to improve the production of nursery plants. The experiment was carried out from January to April 2017 and it was laid out in randomized complete block design with different sizes of cuttings (10, 15 and 20 cm) examined for two cultivars ‘Sawari’ and ‘Tarnab Inzar’, with three replications keeping 20 cuttings per treatment. The variables studied were number of leaves, number of roots, root length, shoot length, sprouting percentage and survival percentage. Cuttings size of fig cultivars had a significant temporal variation in the percentage of successful rooting and survival of cuttings. Based on data regarding number of leaves, number of roots, root length, sprouting percentage, it was concluded that 20 cm long cuttings collected for both cultivars are the best option for commercial production of fig nursery plants.


2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Md. Salim Azad ◽  
Md. Abdul Matin

The study discloses the scopes of clonal propagation Swietenia macrophylla through branch cutting treated with IBA. A total of four hundred cuttings were used with four replications to assess the rooting ability. The study exposed significant (p<0.5) difference of rooted cuttings among the treatments. The highest (62.51%) rooting percent was observed in cutting with 0.4% IBA treatment. In addition, root number and its length per cutting were increased with increasing IBA concentration (p<0.5). The experiment showed significant (p<0.5) difference of sprouting among the treatments. The highest (67.27%) percent of sprouting was observed in cuttings with 0.4% solution, which showed a similar fashion of percent of rooting. The study also showed significant (p<0.5) difference of shoot number per cutting, the length of the longest shoot, and number of leaves per cutting among the treatments. The overall survival of rooted cuttings after transfer to polybag significantly (p<0.5) differed among the cuttings treated with different IBA solution. The highest survival percentage (69.67%) was observed in the cuttings rooted with 0.4% IBA treatment and the lowest (55.6%) survival was found in cuttings treated with control. The use of 0.4% IBA treatment is suggested for rooting of juvenile leafy branch cutting of S. macrophylla.


2014 ◽  
Vol 6 (2) ◽  
pp. 163-166 ◽  
Author(s):  
Hamed BABAIE ◽  
Hossein ZAREI ◽  
Kosar NIKDEL ◽  
Mostafa NAJAR FIROOZJAI

In this research, in order to study the effect of different concentrations of IBA and time of taking cutting on rooting, growth and survival of F. binnendijkii ‘Amstel Queen’ cuttings, a test was conducted in a randomized complete block design with four replications. Treatments were consisted of four levels of hormones: 0 (control), 2000, 4000 and 6000 mg/l, with two intervals of time for taking cuttings (late June and early September). Results showed that highest root percentage, root number and fresh weight of root were for IBA concentrations of 4000 and 6000 mg/l and cuttings taken early in September. The maximum of mean length of root and longest root were achieved in concentration of 6000 mg/l IBA, taking cutting in early September. The highest survival percentage of plants was recorded in concentrations of 4000 and 6000 mg/l IBA, early September being the appropriate time of taking the cutting. The greatest length of new shoots was obtained in concentration of 4000 mg/l IBA, cutting in late June, while the maximum number of new leaf was in IBA concentration of 2000 and 4000 mg/l, with cuttings taken also in late June.


2021 ◽  
Vol 43 ◽  
pp. e50289
Author(s):  
André Schoffel ◽  
Sidinei José Lopes ◽  
Jana Koefender ◽  
Alessandro Dal'Col Lúcio ◽  
Juliane Nicolodi Camera ◽  
...  

A study was conducted during four growing seasons to investigate the rooting capacity and survival percentage of cassava seedlings from stem cuttings selected based on their number of leaves. The experimental design was a randomized block design with five replications in a factorial scheme (4 × 2 × 2), totalling 16 treatments. Treatments consisted of combinations of growing season (September 22, October 7 and 19, and November 25), stem cuttings smaller (15 to 19.99 mm) and larger (20 to 25 mm) than 20 mm in diameter, and number of leaves on the stem cuttings (3 to 5 and 6 to 8 leaves). The experiment was carried out during the four growing seasons in a Van der Hoeven greenhouse. The greenhouse, as well as the benches on which the treatments were placed, had a north-south orientation. The greenhouse has an automatic mist irrigation system, and the mean temperature was 25 °C. The plant height at planting, number of leaves at planting, and number of leaves at 7 days after planting were measured. The final number of leaves, final plant height, shoot dry matter, root dry matter, and total dry matter were measured after acclimatization. Stem cuttings between 20 and 25 mm in diameter should be used to produce cassava seedlings with the rapid multiplication method. Cuttings with 6 to 8 visible leaves should be collected, and the cuttings should be planted at the end of September.


2018 ◽  
Vol 29 (2) ◽  
pp. 99-106 ◽  
Author(s):  
ST Islam ◽  
MZ Haque ◽  
MM Hasan ◽  
ABMMM Khan ◽  
UK Shanta

An experiment was conducted at the research field of the Department of Agricultural Botany, Patuakhali Science and Technology University, Patuakhali under the Agro-ecological zone-13 (AEZ-13) during the period from November 2012 to February 2013. The aim of the study was to identify the most effective irrigation level for obtaining the better growth and higher yield performance of BARI Gom-25 that are suitable to cultivate under  coastal region of Patuakhali, Bangladesh. So, the present study was consists of five irrigations levels including control viz. control or no irrigation (T0), one irrigation at 25 DAS (T1), two irrigations at 25 and 40 DAS (T2), three irrigations at 25, 40 and 55 DAS (T3)  and four irrigations at 25, 40, 55 and 70 DAS (T4). The seeds of BARI Gom-25 were collected from the BARI, Joydebpur, Gazipur on 22 November, 2012. The experiment was laid out in completely randomized block design (RCBD) with four replications and analysis was done by the MSTAT-C package program where means were separated by DMRT at 5% level of probability. Data were recorded on various growth and yield attributing traits. The plot size was 5 m2 (2.5 × 2.0 m) where row to row and seed to seed distances were 20 and 10 cm respectively. Data were collected on various morpho-physiological growth, yield and yield attributing traits. Results obtained from the present study, whole characters of the study were significant at 5% by the moisture (irrigation) levels where three irrigations given at 25, 40 and 55 DAS had most effective than that of other moisture levels and no irrigation. From the results investigation, it was found that the tallest plant (76.86 cm), maximum requiring days to anthesis (61.00 days), maturity (109.0 days) and maximum number of effective tillers (5.00 hill-1),the highest grain growth (3.11g at 36 DAA) and grains (44.00 spike-1) were obtained with three irrigation (T3) levels. Similarly, T3 further showed the greater performance on spike length (17.28 cm), 1000-seed weight (50.16 g), grain (4.16 t ha-1), straw (5.89 t ha-1) and biological yield (10.05 t ha-1) as well as the higher harvest index (41.39%). Investigated above whole characters were produced lower performances under no moisture (irrigation) treatments. These results indicated that irrigation at three times (T3) would be most advantageous irrigation levels for wheat production under the studied non saline ecosystem of coastal region.Progressive Agriculture 29 (2): 99-106, 2018


2021 ◽  
Author(s):  
Yunjie Shi ◽  
Mengke Yin ◽  
Zijue Zhu ◽  
Shun Wang ◽  
Panting Niu ◽  
...  

Abstract In the research field of fiber-optic hydrophone, the performance of demodulation scheme is crucial. In this work, a phase-generated-carrier (PGC) demodulation scheme based on high-frequency sound source is proposed. Highfrequency acoustic signal from the external sound source is applied to the fiber-optic hydrophone to achieve phase modulation of the interference signal instead of the piezo-electrical transducer (PZT) or frequency-modulated laser. It possesses the merits of low system complexity and low cost. Through the acoustic detection experiment, we achieve demodulation of acoustic signal at frequency varying from 300 Hz to 800 Hz, and the signal-to-noise ratio (SNR) is higher than 45 dB. Furthermore, the proposed scheme is successfully applied to time division multiplexing (TDM) experiment.


2017 ◽  
Vol 4 (3) ◽  
pp. 102-107
Author(s):  
Darda Efendi ◽  
Mirza R Putra

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.


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