scholarly journals Isolasi dan Identifikasi Bakteri Pelarut Fosfat pada Tanah Ultisol di Kecamatan Rumbai, Pekanbaru

2020 ◽  
Vol 4 (1) ◽  
pp. 22
Author(s):  
Oksana Oksana ◽  
Mokhamad Irfan ◽  
Annisa Ramadhani Fianiray ◽  
Syukria Ikhsan Zam
Keyword(s):  
Bacterial Cell ◽  
Research Method ◽  
Phosphate Solubilization ◽  
Animal Husbandry ◽  
Phosphate Solubilizing Bacteria ◽  
Biochemical Characteristics ◽  
Cell Numbers ◽  
Teak Plantations ◽  
Phosphate Solubilizing ◽  
Descriptive Method

<p class="Abstract">Phosphate-solubilizing bacterial can fulfill the low available of soluble P on Ultisol. This research aimed to study on the quantity of the population bacteria and identify phosphate-solubilizing bacteria on Ultisol from Rumbai District, Pekanbaru. The research method used was descriptive method. Soil sample was collected from teak plantations of PT. Air Jernih, Sub district of Rumbai Pesisir, Pekanbaru and identification of phosphate-solubilizing bacteria was conducted in Laboratory of Pathology, Entomology and Microbiology, Faculty of Agriculture and Animal Husbandry, Universitas Sultan Syarif Kasim State Islamic, Riau from May-September 2017. The observed parameters were bacterial cell numbers, phosphate solubilization index, microscopic and biochemical characteristics. Four isolates were obtained with cell numbers ranging from 4.2 x 10<sup>5</sup> – 7.1 x 10<sup>5</sup> CFU/g of soil. All isolates showed the ability to dissolve phosphate with phosphate solubilization index ranging from 1.16 – 1.57. The four isolates were identified as <em>Klebsiella </em>(IBJ1 and IBJ2), and <em>Acinetobacter </em>(IBJ3 and IBJ4).</p>

scholarly journals ROCK PHOSPHATE SOLUBILIZATION AND ENZYMATIC ACTIVITIES FROM INDIGENOUS BACTERIA ON CASSAVA MILL EFFLUENT CONTAMINATED SOIL

2020 ◽  
Vol 1 (2) ◽  
pp. 37-51
Author(s):  
C. E. Oshoma ◽  
S. O. Nwodo ◽  
I. S. Obuekwe
Keyword(s):  
Acid Phosphatase ◽  
Contaminated Soil ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Enzymatic Activities ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Indigenous Bacteria ◽  
Rock Phosphate Solubilization ◽  
Phosphate Solubilizing

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.

scholarly journals Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

2019 ◽  
Vol 60 (5) ◽  
pp. 985-995
Author(s):  
Yusur Ramzi ◽  
Hutaf A. A. Alsalim
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Mung Bean ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Hydrolytic Enzymes ◽  
Phosphate Solubilizing Bacteria ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and  of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 μg.ml-1), Y4 was the highest (39.00 μg.ml-1) followed by Y3 (37.00μg.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

scholarly journals Phosphate Solubilization Potentials of Acinetobacter Strains and their Relations with Soil Properties

2010 ◽  
Vol 12 (3,4) ◽  
pp. 231 ◽  
Author(s):  
M. Ogut ◽  
F. Er ◽  
N. Kandemir
Keyword(s):  
Phosphate Solubilization ◽  
Soil Phosphorus ◽  
Soil Samples ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Batch Cultures ◽  
Phosphate Solubilizers ◽  
Gene Sequence Analysis ◽  
Initial Ph ◽  
Phosphate Solubilizing

<p>Phosphate solubilizing bacteria can be used as soil or seed inoculum to increase soil phosphorus (P) availability for agricultural purposes. There is also a possibility of using these microorganisms to biotechnologically dissolve phosphate ores for the production of phosphorus fertilizers. Twenty-one soil samples were collected along a highway in Turkey to isolate phosphate solubilizing bacteria. A total of 20 phosphate solubilizers were isolated from the rhizosphere of wheat and maize grown in the pots, which contained the collected soil samples. The isolates were distributed among the genera, <em>Acinetobacter</em> (7), <em>Pseudomonas</em> (7), <em>Enterobacter</em> (2), <em>Enterococcus</em> (1), <em>Escherichia</em> (1), <em>Photorhabdus</em> (1), and <em>Bacillus</em> (1) as determined by the 16S rDNA gene sequence analysis. Since the <em>Acinetobacter</em> species were most effective in Pikovskaya’s agar, which contained tricalcium phosphate for the sole P-source, they were further experimented for the phosphate solubilization in batch cultures. The mean phosphorus dissolved in 5 day incubation ranged between 167 and 1022 ppm P. The initial pH of 7.8  dropped below 4.7 in six isolates with a gluconic acid production in the concentrations ranging between 27.5 and 37.5 mM. <em>Acinetobacter</em> isolates have some potential as an inoculum both for soil and biotechnological P-solubilization.</p>

scholarly journals Inorganic Phosphate Solubilization by Rhizosphere Bacterium Paenibacillus sonchi: Gene Expression and Physiological Functions

2020 ◽  
Vol 11 ◽  
Author(s):  
Luciana F. Brito ◽  
Marina Gil López ◽  
Lucas Straube ◽  
Luciane M. P. Passaglia ◽  
Volker F. Wendisch
Keyword(s):  
Gene Expression ◽  
Organic Acids ◽  
Phosphate Solubilization ◽  
Tricarboxylic Acid Cycle ◽  
Initial Step ◽  
High Rate ◽  
Soluble Phosphate ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Solubilizing ◽  
Phosphate Source

Due to the importance of phosphorus (P) in agriculture, crop inoculation with phosphate-solubilizing bacteria is a relevant subject of study. Paenibacillus sonchi genomovar Riograndensis SBR5 is a promising candidate for crop inoculation, as it can fix nitrogen and excrete ammonium at a remarkably high rate. However, its trait of phosphate solubilization (PS) has not yet been studied in detail. Here, differential gene expression and functional analyses were performed to characterize PS in this bacterium. SBR5 was cultivated with two distinct P sources: NaH2PO4 as soluble phosphate source (SPi) and hydroxyapatite as insoluble phosphate source (IPi). Total RNA of SBR5 cultivated in those two conditions was isolated and sequenced, and bacterial growth and product formation were monitored. In the IPi medium, the expression of 68 genes was upregulated, whereas 100 genes were downregulated. Among those, genes involved in carbon metabolism, including those coding for subunits of 2-oxoglutarate dehydrogenase, were identified. Quantitation of organic acids showed that the production of tricarboxylic acid cycle-derived organic acids was reduced in IPi condition, whereas acetate and gluconate were overproduced. Increased concentrations of proline, trehalose, and glycine betaine revealed active osmoprotection during growth in IPi. The cultivation with hydroxyapatite also caused the reduction in the motility of SBR5 cells as a response to Pi depletion at the beginning of its growth. SBR5 was able to solubilize hydroxyapatite, which suggests that this organism is a promising phosphate-solubilizing bacterium. Our findings are the initial step in the elucidation of the PS process in P. sonchi SBR5 and will be a valuable groundwork for further studies of this organism as a plant growth-promoting rhizobacterium.

scholarly journals Isolation and characterization of insoluble inorganic phosphate solubilizer rice rhizosphere strain Enterobacter cloacae BAU3

2018 ◽  
Vol 10 (4) ◽  
pp. 1204-1209
Author(s):  
Mahendra Singh
Keyword(s):  
Phosphate Solubilization ◽  
Enterobacter Cloacae ◽  
Phosphate Solubilizing Bacteria ◽  
Rice Rhizosphere ◽  
Phosphate Solubilizers ◽  
Isolation And Characterization ◽  
Agricultural Chemistry ◽  
Phosphate Solubilizing ◽  
Halo Zone

The objective of the present study was to isolate and characterize most efficient phosphate solubilizing bacteria (PSB) from rice rhizosphere. The study was carried out during the Kharif season’2018 at Department of Soil Science and Agricultural Chemistry, Bihar Agricultural University, Sabour, Bhagalpur, Bihar. The availability of phosphorous to plants for uptake and utilization is limited in soil due to fixation in the form of Fe-P, Al-P and Ca-P. The use of phosphate solubilizing bacteria can prove to be helpful measure to supply phosphorous to the crops to increase the productivity. In the present investigation, a total of 10 isolates were obtained from rice rhizosphere soil samples. All ten isolated isolates were shown phosphorus solubilization. Out of ten isolates BAU3 was found to be most potent phosphate solubilizers showing clear halo zone around its colony. The isolate BAU3 showed 20.00 mm phosphate solubilizing halo zone around its colony. The solubilization index (SI) of the isolate BAU3 was also calculated at the end of the incubation period and observed phosphate solubilization index (SI) of 3.22.  The isolate BAU3 showed maximum insoluble phosphate solubilization of 450.24 ?g ml-1 and isolates BAU3 was selected for subsequent studies. The bacterial isolates BAU3 was gram negative, non-spore forming rods shaped. On the basis of the 16SrDNA sequencing, isolate BAU3 was identified as Enterobacter cloacae strain BAU3 (Genebank Accession No.   MK033472). The isolated strain of bacterial has potential to solubilize insoluble phosphorus and it can be utilized for preparation of microbial inoculants or biofertilizers.

scholarly journals Phosphate solubilizing activity of Pseudomonas fluorescens PSM1 isolated from wheat rhizosphere

2016 ◽  
Vol 8 (1) ◽  
pp. 93-96 ◽  
Author(s):  
Anurag Yadav ◽  
Kusum Yadav ◽  
Anupam Vashistha
Keyword(s):  
Pseudomonas Fluorescens ◽  
Phosphate Solubilization ◽  
Ph Value ◽  
Culture Collection ◽  
Phosphate Solubilizing Bacteria ◽  
Linear Regression Equation ◽  
Wheat Rhizosphere ◽  
Bacterial Treatment ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity

A study was designed to screen and analyze the efficient phosphate solubilizing bacteria (PSBs) from wheat rhizosphere. Five biovars of Pseudomonas fluorescens (PSM1, PSM2, PSM3, PSM4 and PSM5) were isolated from wheat rhizosphere and Bacillus megaterium MTCC 8755 procured from microbial type culture collection (MTCC) Chandigarh, India. The P. fluorescens biovar PSM1 was observed to be most efficient phosphate solubilizer. Inoculation of P. fluorescens PSM1 and B. megaterium MTCC 8755, alone and in combination, caused the highest phosphate solubilization at pH 5. At this pH, maximum phosphate solubilization was observed with B. megaterium MTCC 8755 inoculation (8.2 mg mL-1) on sixth day of incubation with P. fluorescens PSM1 (8 mg mL-1) on seventh day of incubation and with dual bacterial treatment (10.5 mg mL-1) on the fourth day of incubation. A correlation coefficient of linear regression equation of phosphate solubilization with pH indicated that pH value of the medium was directly correlated with tricalcium phosphate solubilization. The study will help in choosing soil pH specific PSB inoculant for optimizing plant growth.

scholarly journals Inorganic and organic phosphate solubilization potential of Stenotrophomonas maltophilia

2021 ◽  
Vol 948 (1) ◽  
pp. 012054
Author(s):  
Suliasih ◽  
S Widawati
Keyword(s):  
Acid Phosphatase ◽  
Stenotrophomonas Maltophilia ◽  
Phosphate Solubilization ◽  
Peat Soil ◽  
Organic Phosphate ◽  
Broth Culture ◽  
Phosphate Solubilizing Bacteria ◽  
Organic P ◽  
Phosphate Solubilizing ◽  
Inorganic And Organic

Abstract Most soils contain Phosphorus (P) in insoluble compounds as organic and inorganic forms which is unavailable to plants. Furthermore, phosphate solubilizing bacteria (PSB) play an important role in converting insoluble P to a bioavailable form through solubilizing and mineralizing inorganic or organic P. Therefore, this study aims to determine the ability of isolate a phosphate solubilizing bacterium (PSB), Stenotrophomonas maltophilia to solubilize inorganic and organic P. The bacterium was isolated from peat soil of West Kalimantan using Pikovskaya medium added with Ca3 (PO4)2 as the P source. The activity of organic P (acid phosphatase and phytase) was measured using p-nitrophenyl phosphate disodium (pNPP 0.115 M) and sodium phytate as the substrate of bacterial broth culture. Also, the optimization for inorganic and organic P solubilization by the bacterium was conducted. The maximum values of inorganic and organic phosphate solubilization was recorded to be 52.26 μg/mL and 44.51 U/mL (acid phosphatase), 0.13 U/mL (phytase) respectively. Optimum conditions found were temperature at 30 °C, pH 6.0 and in the presence of sucrose and beef extract, which serve as carbon and nitrogen sources, respectively. Therefore, it can probably be used as a biological fertilizer for plants.

scholarly journals Isolation, characterilzation and identification of phosphate solubilizing bacteria from rice-shrimp rotational farming system in salt affected soil areas of the mekong delta of Vietnam

2018 ◽  
Vol 15 (1) ◽  
pp. 121-131
Author(s):  
Nguyễn Khởi Nghĩa ◽  
Nguyễn Thị Kiều Oanh
Keyword(s):  
Bacterial Strain ◽  
Mekong Delta ◽  
Farming System ◽  
Salinity Range ◽  
Rrna Gene ◽  
Rice Soil ◽  
Phosphate Solubilizing Bacteria ◽  
Cell Numbers ◽  
Salt Affected Soil ◽  
Phosphate Solubilizing

Phosphorus is the least mobile element in plant and soil contrary to other macronutrients. Phosphorus solubilizing bacteria play role in phosphorus nutrition by enhancing its availability to plants. Aim of study was to deal with isolation, characterization and identification of phosphate solubilizing bacteria (PSBs) from 15 paddy rice soil samples collected in rice–shrimp rotational farming system in salt affected soil areas of the Mekong Delta of Vietnam including Bac Lieu, Ca Mau, Soc Trang, Kien Giang and Ben Tre by using NBRIP media containing 1% NaCl. Concentration of phosphate was determined by method of colometric determination of molybdate. Results showed that 95 isolates indicating potentially as PSBs through a exhibiting of a halo zone happening around bacterial colonies on NBRIP media. Twenty out of ninety five isolates showed their highly phosphate solubilizing ability (>1000 mg.L-1) in the liquid culture. Bacterial strain coded as BL1-10 solubilized maximumly 2044 mg.L-1 phosphate in liquid media after 5 incubation days. The cell number and phosphate solubilizing ability of Bl-10 strain were till good and not affected under environmental coditions like 40oC, salinity range between 0.5 and 5 % NaC and pH range between 3 and 5. Three kinds of trade name fungicides like DA roral, Topsin M và Antracol 70WP did not affect on cell numbers and phosphate solubilizing ability of Bl-10 strain. However, cell numbers and function of phosphate solubilizing ability of this bacterial strain was completely inhibited by Ridomil Gold fungicide. This strain was genetically identified as species of Burkholderia sp. BL1-10 based on results of the 16S rRNA gene sequence analysis. In conclusion, the study suggests the most promising phosphate solubilizing bacteria, Burkholderia sp. BL1-10 can be used as efficient biofertilizer inoculant to promote plant growth.

scholarly journals Isolation of phosphate solubilizing Pseudomonas strains from apple rhizosphere in the Trans Himalayan region of Himachal Pradesh, India

2017 ◽  
Author(s):  
Ranjna Sharma ◽  
Joginder Pal ◽  
Mohinder Kaur
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Hplc Analysis ◽  
Apple Tree ◽  
Phosphate Solubilization ◽  
Himachal Pradesh ◽  
Phosphate Solubilizing Bacteria ◽  
16S Rrna Analysis ◽  
Pgp Traits ◽  
Isolation And Characterization ◽  
Phosphate Solubilizing

AbstractTotal fifteen phosphate solubilizing bacteria were isolated from rhizosphere soil of apple tree on Kings’ B medium belonging to genus Pseudomonas spp. They were characterized on the basis of morphological and biochemical characteristics. Preliminary selection as phosphate solubilizing bacteria were done on the basis of formation of transparent zone around the colony on Pikovskaya’s agar medium containing 0.5% triclcium phosphate (TCP). Maximum in vitro phosphate solubilization on Pikovskaya’s agar plates after 72h incubation at 28°C was shown by An-15-Mg (46 mm), whereas in case of broth, again this strain showed maximum tricalcium phosphate (TCP) solubilization (76 μg/ml). The pH of each inoculated broth was recorded daily and dropped significantly (pH 7.0-3.99). All the Pseudomonas isolates were further evaluated for overall plant growth promoting traits. Greatest siderophore activity was exhibited by An-14-Mg (71.23 %SU) followed by An-15-Mg (70.12 %SU) which were statiscally at par with each other. Whereas, maximum IAA production was observed again in An-15-Mg (95 μg/ml). This isolate also showed the maximum production of HCN and ammonia. 16S rDNA and phylogenetic analysis showed that strain An-15-Mg exhibits 99% level of similarity with Pseudomonas aeruginosa. Therefore, it was designated as Pseudomonas aeruginosa strain An-15-Mg. HPLC analysis showed that the Pseudomonas aeruginosa strain An-15-Mg produced maximum concentration of succinic acid, malonic, citric and malic acid with small amounts of schimic, quinic, tartaric, fumaric and lactic acids. The strain An-15-Mg possessed phosphate solubilization as major PGP trait along with different PGP traits. The potential phosphate solubilizing strain of Pseudomonas aeruginosa was reported first time as PGPR which lives in close association with apple tree without harming the plant, therefore could be used as a promising phosphate solubilizer and biofertilizer in apple crop grown in high hills of Himachal Pradesh.Authors contributionAll authors made significant efforts towards the completion of this research work and preparation of manuscript timely. Author Ranjna Sharma has done collection of soil samples of apple tree from two districts, isolation and characterization of phosphate solubilizing Pseudomonas isolates and their screening for different PGPT’s, phenotypic characteriazation, 16S rRNA analysis and statistical analysis. Author S P Singh has done HPLC analysis at their institute. Author Ankita has done sequence analysis and phylogenetic tree preparation. Author Mohinder Kaur is my mentor and generated idea regarding this work. She guided me in completing this work successfully as well as manuscript writing.

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