scholarly journals BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

2016 ◽  
Vol 12 (1) ◽  
pp. 70 ◽  
Author(s):  
Eli Rohaeti ◽  
Endang W Laksono ◽  
Anna Rakhmawati
Keyword(s):  
Waste Water ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Nitrate Solution ◽  
Diffusion Method ◽  
E Coli ◽  
Vis Spectroscopy ◽  
Chitosan Composite

<pre><span lang="EN-GB">Bacterial cellulose (C) and its composites were synthesized from rice waste water<strong> </strong>with addition of glycerol (G) and chitosan (Ch).</span><strong></strong><span lang="EN-GB">Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial  cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against <em>S. aureus</em>, <em>E.</em> <em>coli</em>,  and yeast <em>C. albicans</em> has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against <em>S</em>. <em>aureus</em>, <em>E. coli</em>, and <em>C. albicans</em> by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited  the silver nanoparticles against of <em>S. aureus</em>, <em>E. coli</em>,  and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the <em>Staphylococcus aureus</em> ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the <em>E. coli</em> ATCC 25922 and the yeast <em>Candida albicans</em> ATCC 10231.</span></pre>

scholarly journals BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

2016 ◽  
Vol 12 (1) ◽  
pp. 70 ◽  
Author(s):  
Eli Rohaeti ◽  
Endang W Laksono ◽  
Anna Rakhmawati
Keyword(s):  
Waste Water ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Nitrate Solution ◽  
Diffusion Method ◽  
E Coli ◽  
Vis Spectroscopy ◽  
Chitosan Composite

<pre><span lang="EN-GB">Bacterial cellulose (C) and its composites were synthesized from rice waste water<strong> </strong>with addition of glycerol (G) and chitosan (Ch).</span><strong></strong><span lang="EN-GB">Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial  cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against <em>S. aureus</em>, <em>E.</em> <em>coli</em>,  and yeast <em>C. albicans</em> has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against <em>S</em>. <em>aureus</em>, <em>E. coli</em>, and <em>C. albicans</em> by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited  the silver nanoparticles against of <em>S. aureus</em>, <em>E. coli</em>,  and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the <em>Staphylococcus aureus</em> ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the <em>E. coli</em> ATCC 25922 and the yeast <em>Candida albicans</em> ATCC 10231.</span></pre>

BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Eli Rohaeti
Keyword(s):  
Waste Water ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Nitrate Solution ◽  
Diffusion Method ◽  
E Coli ◽  
Vis Spectroscopy ◽  
Chitosan Composite

<pre><span lang="EN-GB">Bacterial cellulose (C) and its composites were synthesized from rice waste water<strong> </strong>with addition of glycerol (G) and chitosan (Ch).</span><strong></strong><span lang="EN-GB">Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial  cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against <em>S. aureus</em>, <em>E.</em> <em>coli</em>,  and yeast <em>C. albicans</em> has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against <em>S</em>. <em>aureus</em>, <em>E. coli</em>, and <em>C. albicans</em> by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited  the silver nanoparticles against of <em>S. aureus</em>, <em>E. coli</em>,  and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the <em>Staphylococcus aureus</em> ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the <em>E. coli</em> ATCC 25922 and the yeast <em>Candida albicans</em> ATCC 10231.</span></pre>

scholarly journals Bacterial Cellulose From Rice Waste Water With Addition Chitosan, Glycerol, And Silver Nanoparticle

Molekul ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. 9
Author(s):  
Eli Rohaeti ◽  
Endang WLFX ◽  
Anna Rakhmawati
Keyword(s):  
Waste Water ◽  
Particle Size ◽  
Silver Nanoparticles ◽  
Bacterial Cellulose ◽  
Silver Nanoparticle ◽  
Nitrate Solution ◽  
Glycerol Solution ◽  
Antibacterial Test ◽  
E Coli ◽  
Vis Spectroscopy

This study aimed to prepare silver nanoparticles chemically, deposite silver nanoparticles on bacterial cellulose-chitosan-glycerol composite based rice waste water, as well as test the antibacterial activity of bacterial cellulose and its composite. Preparation of silver nanoparticles was conducted by chemical reduction of silver nitrate solution, as well as trisodium citrate as the reductor. Bacterial cellulose from rice waste water is fermented by the bacteria Acetobacter xylinum for 7 days. The dried bacterial cellulose was composited with chitosan and glycerol by immersion method on 2% of chitosan solution and 0.5% of glycerol solution. UV-Vis spectroscopy is used to determine the formation of silvernanoparticles and Particle Size Analyzer to test the size and particle size distribution. Characterization was conducted to bacterial cellulose and its composite included functional groups by FTIR, the mechanical properties by Tensile Tester, crystallinity by XRD, surface photograph by SEM, and antibacterial test against S. aureus and E. coli by the shake flask turbidimetry method. Silver nanoparticle characterization indicated that silver nanoparticles are formed at a wavelength of 421.80 nm, yellow, diameter particle size of 61.8 nm. SEM images showed that the surface of bacterial cellulose had deposited silver nanoparticles and antibacterial test showed an inhibitory effect of bacterial cellulose, bacterial cellulose-chitosan composite, and bacterial cellulose-chitosan-glycerol composite which are deposited silver nanoparticles against the growth of S. aureus and E. coli bacteria.

scholarly journals Potential antibacterial activity of crude extracts and silver nanoparticles synthesized from Sargassum wightii

2014 ◽  
Vol 3 (10) ◽  
pp. 322-325 ◽  
Author(s):  
Vinoth Kumar Thirumalairaj ◽  
Mahitha Puthanpurayal Vijayan ◽  
Geetharamani Durairaj ◽  
Lakshmanasenthil Shanmugaasokan ◽  
Rincy Yesudas ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Zone Of Inhibition ◽  
Ag Nps ◽  
Sargassum Wightii ◽  
Solvent Extract ◽  
Vis Spectroscopy

The present work investigates the antibacterial activity of silver nanoparticles (Ag-NPs) synthesized by biological method using Sargassum wightii. The fresh live seaweed was collected from the Mandapam coast of Tamilnadu, India. Solvent extract was prepared using acetone, petroleum ether and methanol. Aqueous extract of the seaweed was also used for the synthesis of silver Ag-NPs. Seaweed extract is used as a reducing agent of 2mM silver nitrate solution for the synthesis of Ag-NPs. Periodical monitoring of reaction mixture was done using UV-vis spectroscopy at 300-750 nm. The scanning electron microscopy (SEM) of the sample confirms the presence of Ag-NPs. The antibacterial activity of solvent extract was done by Minimal inhibitory concentration (MIC) assay. The methanol extract of the seaweed at a concentration of 250µg/ml exhibited potent antimicrobial activity against the test microorganism. The zone of inhibition ranging from 8-14 mm was observed with different extracts. The antibacterial activity of the synthesized Ag-NPs against the organism was also done by MIC test. The MIC of Ag-NPs was found to be 130µg/ml for all pathogenic microorganisms selected for the study. The zone of inhibition against Bacillus cereus, Bacillus anhtracis, Staphylococcus aureus and Vibrio alginoyticus were found to be 10, 8, 10 and 9 mm, respectively. The synthesized Ag-NPs exhibited significant antimicrobial activity against the selected microorganisms than the solvent extract of seaweed.DOI: http://dx.doi.org/10.3329/icpj.v3i10.20337 International Current Pharmaceutical Journal, September 2014, 3(10): 322-325

scholarly journals Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mona A. Alqahtani ◽  
Monerah R. Al Othman ◽  
Afrah E. Mohammed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Synergistic Effect ◽  
Cancer Cell ◽  
Spherical Particles ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
Vis Spectroscopy ◽  
Hct 116

Abstract Recently, increase bacterial resistance to antimicrobial compounds issue constitutes a real threat to human health. One of the useful materials for bacterial control is Silver nanoparticles (AgNPs). Researchers tend to use biogenic agents to synthesize stable and safe AgNPs. The principal aim of this study was to investigate the ability of lichen in AgNPs formation and to find out their suppression ability to MDR bacteria as well as their cytotoxic activity. In the current study, lichens (Xanthoria parietina, Flavopunctelia flaventior) were collected from the south of the Kingdom of Saudi Arabia. Lichens methanolic extracts were used for conversion of Ag ions to AgNPs. Prepared biogenic AgNPs were characterized by Ultraviolet–Visible (UV–Vis) Spectroscopy, Transmission electron microscopy (TEM), Dynamic Light Scattering (DLS) and Zeta potential and Energy-Dispersive X-ray Spectroscopy (EDS). Lichens Secondary metabolites were determined by Fourier-Transform Infrared Spectroscopy (FTIR) and Gas Chromatography–Mass Spectrometry (GC–MS). The antibacterial activity and synergistic effect of AgNPs were evaluated against pathogenic bacteria, including gram-positive; Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-resistant Enterococcus (VRE), and gram-negative; (Pseudomonas aeruginosa, Escherichia coli) as well as the reference strains (ATCC) using the agar disk diffusion method. Cytotoxic effect of biogenic AgNPs was tested against HCT 116 (Human Colorectal Cancer cell), MDA-MB-231 (Breast cancer cell), and FaDu (Pharynx cancer cell) by MTT test. TEM imaging showed well-dispersed spherical particles of 1–40 nm size as well as zeta size showed 69–145 nm. Furthermore, FTIR and GC–MS identified various lichen chemical molecules. On the other hand, the highest antibacterial activity of AgNPs was noticed against P. aeruginosa, followed by MRSA, VRE, and E. coli. AgNPs influence on gram-negative bacteria was greater than that on gram-positive bacteria and their synergistic effect with some antibiotics was noted against examined microbes. Moreover, higher cytotoxicity for biogenic AgNPs against FaDu and HCT 116 cell line in relation to MDA-MB-231 was noted. Given the current findings, the biogenic AgNPs mediated by lichens had positive antibacterial, synergistic and cytotoxic powers. Therefore, they might be considered as a promising candidate to combat the multi-drug resistance organisms and some cancer cells.

scholarly journals Antibacterial Activity of TiO2- and ZnO-Decorated with Silver Nanoparticles

2019 ◽  
Vol 3 (2) ◽  
pp. 61 ◽  
Author(s):  
Van Thang Nguyen ◽  
Viet Tien Vu ◽  
The Huu Nguyen ◽  
Tuan Anh Nguyen ◽  
Van Khanh Tran ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Zno Nanoparticles ◽  
Visible Region ◽  
Light Irradiation ◽  
Hybrid Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Vis Spectroscopy ◽  
Xrd Patterns

This work emphasizes the use of the silver decorative method to enhance the antibacterial activity of TiO2 and ZnO nanoparticles. These silver-decorated nanoparticles (hybrid nanoparticles) were synthesized using sodium borohydride as a reducing agent, with the weight ratio of Ag precursors/oxide nanoparticles = 1:30. The morphology and optical properties of these hybrid nanoparticles were investigated using transmission electron microscopy (TEM), X-ray diffraction (XRD) patterns, and UV-Vis spectroscopy. The agar-well diffusion method was used to evaluate their antibacterial activity against both Staphylococcus aureus and Escherichia coli bacteria, with or without light irradiation. The TEM images indicated clearly that silver nanoparticles (AgNPs, 5–10 nm) were well deposited on the surface of nano-TiO2 particles (30–60 nm). In addition to this, bigger AgNPs (<20 nm) were dispersed on the surface of nano-ZnO particles (30–50 nm). XRD patterns confirmed the presence of AgNPs in both Ag-decorated TiO2 and Ag-decorated ZnO nanoparticles. UV-Vis spectra confirmed that the hybridization of Ag and oxide nanoparticles led to a shift in the absorption edge of oxide nanoparticles to the lower energy region (visible region). The antibacterial tests indicated that both oxide pure nanoparticles did not exhibit inhibitory effects against bacteria, with or without light irradiation. However, the presence of AgNPs in their hybrids, even at low content (<40 mg/mL), leads to a good antibacterial activity, and higher inhibition zones under light irradiation as compared to those in dark were observed.

Biological Applications, Green Synthesis, Characterization of Silver Nanoparticles (AgNPs) Using Artemisia annua L.

2021 ◽  
Vol 13 (7) ◽  
pp. 1304-1309
Author(s):  
Hamed A. Ghramh ◽  
Rahmah N. Al-Qthanin ◽  
Zubair Ahmad ◽  
Essam H. Ibrahim ◽  
Mona Kilany ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Artemisia Annua ◽  
Diffusion Method ◽  
Biological Applications ◽  
Disk Diffusion Method ◽  
Vis Spectroscopy ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis

ABSTRACTThis article reports on the silver nanoparticles (AaAgNPs) that were green-synthesized by using Artemisia annua L. extract (AaExt) and their collective biological applications. Active biomolecules in the extract and extract containing AgNPs were characterized using Fourier-transform-infrared-spectroscopy (FTIR) and AgNPs were monitored by UV/vis spectroscopy and SEM (scanning electron microscopy) analysis. The size of the particle is around 100 nm. The antibacterial activity was measured by the disk diffusion method against the Gram-negative/positive pathogenic bacteria. The extract and extract containing AgNPs showed a significant antibacterial activity. Cytotoxic potential of the synthesized AgNPs was analyzed against the rat splenocytes. The results showed that there were cytotoxic effects of A. annua leaves extract but stimulatory effects when the extract contained AgNPs on normal splenocytes. Extract of A. annua showed very little increase in liver enzymes. Regarding the larvicidal activity, the extract containing AgNPs was more effective than the crude leaves extract against 4th instar larvae of Culex pipiens (LC50 = 171.378 ppm) compared to the plant extract (LC50 = 5389.726 ppm) by about 31.449 folds.

scholarly journals BIOMIMETIC SYNTHESIS, CHARACTERIZATION AND EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL EFFICACY OF SILVER NANOPARTICLES USING ANDREDERA CORDIFOLIA LEAF EXTRACT

2017 ◽  
Vol 10 (4) ◽  
pp. 329 ◽  
Author(s):  
Rajathi Palanivelrajan ◽  
Suja S
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Biomimetic Synthesis ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Frap Assay ◽  
Vis Spectroscopy

Objective: The present study is focussed on the biosynthesis of silver nanoparticles using aqueous extract of Andredera cordifolia and to investigate the free radical scavenging potential, antimicrobial activity of the nanoparticles against different human pathogens.Methods: The formation of silver nanoparticles was indicated by the colour change from colourless to reddish brown. Biosynthesized nanoparticles was characterised using several techniques, viz- UV-Vis spectroscopy, FT-IR, XRD, TEM, SEM and EDAX analysis. The free radical scavenging potential was measured by DPPH, FRAP assay, antimicrobial activity against six microorganisms was tested using disc diffusion method.  Results: UV-Vis spectral analysis showed silver Surface Plasmon Resonance band at 426 nm.  The crystalline morphology and size of the nanoparticles were determined by TEM, SEM, and X-ray diffraction studies which showed the average size of the nanoparticles in the range 40 –60nm.  The biologically synthesized nanoparticles efficiently inhibited pathogenic organisms such as E. coli, Staphylococcus aureus, Klebsiella Pneumonia, Pseudomonas aeruginosa and Proteus vulgaris.  The biosynthesized nanoparticles might serve as a potent antioxidant as revealed by DPPH assay and FRAP assay. Conclusion: The biosynthesis of silver nanoparticles had several advantages in pharmaceutical applications as well as large scale commercial production.Key Words: Silver nanoparticle, Andredera cordifolia leaf, SEM,TEM,XRD,antioxidant, antimicrobial,etc.

scholarly journals Fortunella japonica extract as a reducing agent for green synthesis of silver nanoparticles

2018 ◽  
Vol 7 (3) ◽  
pp. 1570
Author(s):  
Nguyen Phung Anh ◽  
Truong Thi Ai Mi ◽  
Duong Huynh Thanh Linh ◽  
Nguyen Thi Thuy Van ◽  
Hoang Tien Cuong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Volume Ratio ◽  
Diffusion Method ◽  
High Antibacterial Activity ◽  
Agno3 Solution ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Average Size

A rapid way of synthesizing silver nanoparticles (AgNPs) by treating Ag+ ions with a green Fortunella Japonica (F.J.) extract as a combined reducing and stabilizing agent was investigated. The reaction solutions were monitored using UV-Vis spectroscopy, the size and shape of crystals were determined by scanning electron microscopy and transmission electron microscopy, the crystalline phases of AgNPs were presented by X–ray diffraction, and the relation of nanoparticles with Fortunella Japonica extract was confirmed using fourier transform infrared spectroscopy. The results indicated that no formation of AgNPs had taken place in the dark during 24 hours at room temperature and 40 oC. Meanwhile, it was found that the rate of AgNPs formation increased rapidly under the sunlight. The effects of the synthesis factors on the AgNPs formation were investigated. The suitable conditions for the synthesis of AgNPs using F.J. extract were determined as follows: F.J. extract was mixed with AgNO3 1.75 mM solution with the volume ratio of 3.5 AgNO3 solution/1.5 F.J. Extract, stirred 300 rpm for 150 minutes at 40 oC under sunlight illumination. At these conditions, AgNPs showed high crystalline structure with the average size of 15.9 nm. The antibacterial activity of silver nanoparticles was determined by agar well diffusion method against E. coli and B. subtilis bacteria. The green synthesized AgNPs performed high antibacterial activity against both bacteria.  

scholarly journals SYNTHESIS, CHARACTERIZATION OF ANTIMICROBIAL ACTIVITY OF 22’DICHLOROHYDROBENZOIN

2018 ◽  
Vol 11 (12) ◽  
pp. 334
Author(s):  
Thanuja B ◽  
Charles Kanagam
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
X Ray ◽  
E Coli ◽  
Vis Spectroscopy ◽  
Optical Behavior ◽  
Thermal Stability Of

Objective: The objective of this work to evaluate the antimicrobial activities of synthesized 22’dichlorohydrobenzoin (22’CD) a new organic crystal.Methods: 22’CD a new organic crystal was grown by vapor diffusion method. Single crystals of 22’CD have been subjected to X-ray diffraction analysis to estimate the lattice parameters and the space group. The molecular structure was confirmed using Fourier transform infrared and nuclear magnetic resonance (NMR) spectral analyses. Optical behavior and thermal stability of the crystal were determined using UV-Vis spectroscopy and thermogravimetry-differential thermal analysis curves. In the present study, antimicrobial activity of 22’CD was evaluated against Escherichia coli and Bacillus subtilis was evaluated by agar well diffusion method.Results: Antibacterial activity of 22’CD was analyzed with ciprofloxacin and miconazole standard and tested against E. coli, Pseudomonas aeruginosa, Salmonella paratyphi, Klebsiella pneumonia’s, Staphylococcus aureus, Streptococcus progenies, and B. subtilis.Conclusion: The 22’CD was found to be effective against E. coli and B. subtitles.

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