scholarly journals Antibacterial Activity of TiO2- and ZnO-Decorated with Silver Nanoparticles

2019 ◽  
Vol 3 (2) ◽  
pp. 61 ◽  
Author(s):  
Van Thang Nguyen ◽  
Viet Tien Vu ◽  
The Huu Nguyen ◽  
Tuan Anh Nguyen ◽  
Van Khanh Tran ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Zno Nanoparticles ◽  
Visible Region ◽  
Light Irradiation ◽  
Hybrid Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Vis Spectroscopy ◽  
Xrd Patterns

This work emphasizes the use of the silver decorative method to enhance the antibacterial activity of TiO2 and ZnO nanoparticles. These silver-decorated nanoparticles (hybrid nanoparticles) were synthesized using sodium borohydride as a reducing agent, with the weight ratio of Ag precursors/oxide nanoparticles = 1:30. The morphology and optical properties of these hybrid nanoparticles were investigated using transmission electron microscopy (TEM), X-ray diffraction (XRD) patterns, and UV-Vis spectroscopy. The agar-well diffusion method was used to evaluate their antibacterial activity against both Staphylococcus aureus and Escherichia coli bacteria, with or without light irradiation. The TEM images indicated clearly that silver nanoparticles (AgNPs, 5–10 nm) were well deposited on the surface of nano-TiO2 particles (30–60 nm). In addition to this, bigger AgNPs (<20 nm) were dispersed on the surface of nano-ZnO particles (30–50 nm). XRD patterns confirmed the presence of AgNPs in both Ag-decorated TiO2 and Ag-decorated ZnO nanoparticles. UV-Vis spectra confirmed that the hybridization of Ag and oxide nanoparticles led to a shift in the absorption edge of oxide nanoparticles to the lower energy region (visible region). The antibacterial tests indicated that both oxide pure nanoparticles did not exhibit inhibitory effects against bacteria, with or without light irradiation. However, the presence of AgNPs in their hybrids, even at low content (<40 mg/mL), leads to a good antibacterial activity, and higher inhibition zones under light irradiation as compared to those in dark were observed.

scholarly journals Effect of Silver Decoration and Light Irradiation on the Antibacterial Activity of TiO2 and ZnO Nanoparticles

2019 ◽  
Author(s):  
Van Thang Nguyen ◽  
Tien Viet Vu ◽  
The Huu Nguyen ◽  
Tuan Anh Nguyen ◽  
Thien Vuong Nguyen ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Zno Nanoparticles ◽  
Visible Region ◽  
Weight Ratio ◽  
Light Irradiation ◽  
Hybrid Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Vis Spectroscopy ◽  
Escherichia Coli Bacteria

This work emphasizes to use silver decorative method to enhance the antibacterial activity of TiO2 and ZnO nanoparticles. These silver decorated nanoparticles (hybrid nanoparticles) were synthesized by using sodium borohydride as a reducing agent, with the weight ratio of Ag precursors: oxide nanoparticles = 1: 30. The morphology and optical property of these hybrid nanoparticles were investigated using transmission electron microscopy (TEM) and UV&ndash;vis spectroscopy. The agar-well diffusion method was used to evaluate their antibacterial activity against both Staphylococcus aureus and Escherichia coli bacteria, with or without light irradiation. The TEM images indicated clearly that silver nanoparticles (AgNPs, 5-10 nm) were well deposited on the surface of nano-TiO2 particles (30-60 nm). Besides, smaller AgNPs (&lt; 2 nm) were dispersed on the surface of nano-ZnO particles (20-50 nm). UV-vis spectra confirmed that the hybridization of Ag and oxide nanoparticles led to shift the absorption edge of oxide nanoparticles to the lower energy region (visible region). The antibacterial tests indicated that both oxide pure nanoparticles did not exhibit inhibitory against bacteria, with or without light irradiation. However, the presence of AgNPs in their hybrids, even at low content (&lt; 40 mg/mL) leads to a good antibacterial activity and the higher inhibition zones under light irradiation as compared to that in dark was observed.

scholarly journals Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mona A. Alqahtani ◽  
Monerah R. Al Othman ◽  
Afrah E. Mohammed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Synergistic Effect ◽  
Cancer Cell ◽  
Spherical Particles ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
Vis Spectroscopy ◽  
Hct 116

Abstract Recently, increase bacterial resistance to antimicrobial compounds issue constitutes a real threat to human health. One of the useful materials for bacterial control is Silver nanoparticles (AgNPs). Researchers tend to use biogenic agents to synthesize stable and safe AgNPs. The principal aim of this study was to investigate the ability of lichen in AgNPs formation and to find out their suppression ability to MDR bacteria as well as their cytotoxic activity. In the current study, lichens (Xanthoria parietina, Flavopunctelia flaventior) were collected from the south of the Kingdom of Saudi Arabia. Lichens methanolic extracts were used for conversion of Ag ions to AgNPs. Prepared biogenic AgNPs were characterized by Ultraviolet–Visible (UV–Vis) Spectroscopy, Transmission electron microscopy (TEM), Dynamic Light Scattering (DLS) and Zeta potential and Energy-Dispersive X-ray Spectroscopy (EDS). Lichens Secondary metabolites were determined by Fourier-Transform Infrared Spectroscopy (FTIR) and Gas Chromatography–Mass Spectrometry (GC–MS). The antibacterial activity and synergistic effect of AgNPs were evaluated against pathogenic bacteria, including gram-positive; Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-resistant Enterococcus (VRE), and gram-negative; (Pseudomonas aeruginosa, Escherichia coli) as well as the reference strains (ATCC) using the agar disk diffusion method. Cytotoxic effect of biogenic AgNPs was tested against HCT 116 (Human Colorectal Cancer cell), MDA-MB-231 (Breast cancer cell), and FaDu (Pharynx cancer cell) by MTT test. TEM imaging showed well-dispersed spherical particles of 1–40 nm size as well as zeta size showed 69–145 nm. Furthermore, FTIR and GC–MS identified various lichen chemical molecules. On the other hand, the highest antibacterial activity of AgNPs was noticed against P. aeruginosa, followed by MRSA, VRE, and E. coli. AgNPs influence on gram-negative bacteria was greater than that on gram-positive bacteria and their synergistic effect with some antibiotics was noted against examined microbes. Moreover, higher cytotoxicity for biogenic AgNPs against FaDu and HCT 116 cell line in relation to MDA-MB-231 was noted. Given the current findings, the biogenic AgNPs mediated by lichens had positive antibacterial, synergistic and cytotoxic powers. Therefore, they might be considered as a promising candidate to combat the multi-drug resistance organisms and some cancer cells.

scholarly journals Synthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Lippia adoensis (Koseret) and Evaluation of Its Antibacterial Activity

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Meron Girma Demissie ◽  
Fedlu Kedir Sabir ◽  
Gemechu Deressa Edossa ◽  
Bedasa Abdisa Gonfa
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Lippia Adoensis

The synthesis of metal oxide nanoparticles with the use of medicinal plant extract is a promising alternative to the conventional chemical method. This work aimed to synthesize zinc oxide nanoparticles using a green approach from indigenous “Koseret” Lippia adoensis leaf extract which is an endemic medicinal plant and cultivated in home gardens of different regions of Ethiopia. The biosynthesized zinc oxide nanoparticles were characterized using thermogravimetric analysis, X-ray diffraction, scanning electron microscopy-energy dispersive spectroscopy, transmission electron microscopy, ultraviolet-visible spectroscopy, and Fourier transform infrared spectroscopy. Furthermore, this study also evaluated the antibacterial activity of the synthesized ZnO nanoparticles against clinical and standard strains of Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Enterococcus faecalis by the disc diffusion method. According to the result of this study, ZnO nanoparticles synthesized using Lippia adoensis leaf extract showed promising result against both Gram-positive and Gram-negative bacterial strains with a maximum inhibition zone of 14 mm and 12 mm, respectively, using uncalcinated form of the synthesized ZnO nanoparticles.

Biological Applications, Green Synthesis, Characterization of Silver Nanoparticles (AgNPs) Using Artemisia annua L.

2021 ◽  
Vol 13 (7) ◽  
pp. 1304-1309
Author(s):  
Hamed A. Ghramh ◽  
Rahmah N. Al-Qthanin ◽  
Zubair Ahmad ◽  
Essam H. Ibrahim ◽  
Mona Kilany ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Artemisia Annua ◽  
Diffusion Method ◽  
Biological Applications ◽  
Disk Diffusion Method ◽  
Vis Spectroscopy ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis

ABSTRACTThis article reports on the silver nanoparticles (AaAgNPs) that were green-synthesized by using Artemisia annua L. extract (AaExt) and their collective biological applications. Active biomolecules in the extract and extract containing AgNPs were characterized using Fourier-transform-infrared-spectroscopy (FTIR) and AgNPs were monitored by UV/vis spectroscopy and SEM (scanning electron microscopy) analysis. The size of the particle is around 100 nm. The antibacterial activity was measured by the disk diffusion method against the Gram-negative/positive pathogenic bacteria. The extract and extract containing AgNPs showed a significant antibacterial activity. Cytotoxic potential of the synthesized AgNPs was analyzed against the rat splenocytes. The results showed that there were cytotoxic effects of A. annua leaves extract but stimulatory effects when the extract contained AgNPs on normal splenocytes. Extract of A. annua showed very little increase in liver enzymes. Regarding the larvicidal activity, the extract containing AgNPs was more effective than the crude leaves extract against 4th instar larvae of Culex pipiens (LC50 = 171.378 ppm) compared to the plant extract (LC50 = 5389.726 ppm) by about 31.449 folds.

scholarly journals Fortunella japonica extract as a reducing agent for green synthesis of silver nanoparticles

2018 ◽  
Vol 7 (3) ◽  
pp. 1570
Author(s):  
Nguyen Phung Anh ◽  
Truong Thi Ai Mi ◽  
Duong Huynh Thanh Linh ◽  
Nguyen Thi Thuy Van ◽  
Hoang Tien Cuong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Volume Ratio ◽  
Diffusion Method ◽  
High Antibacterial Activity ◽  
Agno3 Solution ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Average Size

A rapid way of synthesizing silver nanoparticles (AgNPs) by treating Ag+ ions with a green Fortunella Japonica (F.J.) extract as a combined reducing and stabilizing agent was investigated. The reaction solutions were monitored using UV-Vis spectroscopy, the size and shape of crystals were determined by scanning electron microscopy and transmission electron microscopy, the crystalline phases of AgNPs were presented by X–ray diffraction, and the relation of nanoparticles with Fortunella Japonica extract was confirmed using fourier transform infrared spectroscopy. The results indicated that no formation of AgNPs had taken place in the dark during 24 hours at room temperature and 40 oC. Meanwhile, it was found that the rate of AgNPs formation increased rapidly under the sunlight. The effects of the synthesis factors on the AgNPs formation were investigated. The suitable conditions for the synthesis of AgNPs using F.J. extract were determined as follows: F.J. extract was mixed with AgNO3 1.75 mM solution with the volume ratio of 3.5 AgNO3 solution/1.5 F.J. Extract, stirred 300 rpm for 150 minutes at 40 oC under sunlight illumination. At these conditions, AgNPs showed high crystalline structure with the average size of 15.9 nm. The antibacterial activity of silver nanoparticles was determined by agar well diffusion method against E. coli and B. subtilis bacteria. The green synthesized AgNPs performed high antibacterial activity against both bacteria.  

scholarly journals Biosynthesis of zinc oxide nanoparticles by using fruits extracts of Ananas Comosus and its antibacterial activity

2019 ◽  
Vol 15 (2) ◽  
pp. 268-273 ◽  
Author(s):  
Raja Adibah Raja Ahmad ◽  
Zawati Harun ◽  
Mohd Hafiz Dzarfan Othman ◽  
Hatijah Basri ◽  
Muhamad Zaini Yunos ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Metallic Nanoparticles ◽  
Ananas Comosus ◽  
Biological Synthesis ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
X Ray

Biosynthesis of metallic nanoparticles using plants, enzymes, and microorganism have been known as eco-friendly alternatives to conventional physical and chemical methods. Recently, the biological synthesis of nanoparticles has been a keen interest amongst researchers and scientist due to its simple technique, eco-friendliness, non-toxic, inexpensive and potential to perform in antibacterial activity. Thus, in this current work, the synthesis of zinc oxide (ZnO) nanoparticles using reduction agent from fruit extracts of Ananas Comosus is reported. The biosynthesized zinc oxide was characterized using Field Emission Scanning Electron Microscope (FESEM) with Energy Dispersive X-ray analysis (EDX), UV-Vis absorption spectroscopy and X-ray diffraction (XRD). The average size of the nanoparticles was found to be in the range of 30-57nm. The antibacterial activity of ZnO nanoparticles was carried out via agar diffusion method against pathogenic organisms. It is observed that the biosynthesized ZnO in the process has the efficient antibacterial activity. In conclusion, the green synthesis of zinc oxide nanoparticles using the fruit extract of Ananas Comosus is considered as a potential additive to substitute other metal oxides such as silver (Ag) and titanium dioxide (TiO2)but also provide antibacterial effect that able to enhance the nanoparticle performance.

scholarly journals BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

2016 ◽  
Vol 12 (1) ◽  
pp. 70 ◽  
Author(s):  
Eli Rohaeti ◽  
Endang W Laksono ◽  
Anna Rakhmawati
Keyword(s):  
Waste Water ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Nitrate Solution ◽  
Diffusion Method ◽  
E Coli ◽  
Vis Spectroscopy ◽  
Chitosan Composite

<pre><span lang="EN-GB">Bacterial cellulose (C) and its composites were synthesized from rice waste water<strong> </strong>with addition of glycerol (G) and chitosan (Ch).</span><strong></strong><span lang="EN-GB">Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial  cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against <em>S. aureus</em>, <em>E.</em> <em>coli</em>,  and yeast <em>C. albicans</em> has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against <em>S</em>. <em>aureus</em>, <em>E. coli</em>, and <em>C. albicans</em> by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited  the silver nanoparticles against of <em>S. aureus</em>, <em>E. coli</em>,  and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the <em>Staphylococcus aureus</em> ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the <em>E. coli</em> ATCC 25922 and the yeast <em>Candida albicans</em> ATCC 10231.</span></pre>

Biogenic of silver nanoparticles using ayurveda tooth powder and its antibacterial activity of extended-spectrum beta-lactamases producing E.coli

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Abdullah A. Alarfajj ◽  
Mohammedsaleh Almallahi ◽  
Murugan A. Munusamy ◽  
Mickymaray Suresh ◽  
Wael Alturaiki
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Low Cost ◽  
Diffusion Method ◽  
Tem Analysis ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Extended Spectrum Beta Lactamases

Extended-spectrum beta-lactamases (ESBL) are enzymes produced by E. coli like some gram negative bacteria. The patients who are affected by ESBL producing bacteria facing a major problem and they may need different β- lactam antibiotics to treat the infection. But this extensive use of β- lactam antibiotics against ESPLs creating major public health threat.  As an alternative currently many eco- friendly, non-toxic, low cost nanoparticles are synthesizing by biogenic way used as an alternative for the β- lactam antibiotics. In the present study silver nanoparticles (AgNPs) were synthesized using Ayurveda Toothpowder. The synthesized AgNPs were characterized using ultraviolet (UV)-visible (vis) spectroscopy, scanning electron microscopy (SEM), and transmission electron microscope (TEM) analysis and finally the antibacterial activity was performed against ESBL producing bacteria by well diffusion method. Antibacterial tests against ESPL producing E.coli cells using biogenic synthesized AgNPs showed significant antibacterial effect at low concentration of AgNPs. The results proved that the biogenic synthesised nanoparticles using Toothpowder extract would help to arrest ESBL producing bacteria a

scholarly journals BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

2016 ◽  
Vol 12 (1) ◽  
pp. 70 ◽  
Author(s):  
Eli Rohaeti ◽  
Endang W Laksono ◽  
Anna Rakhmawati
Keyword(s):  
Waste Water ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Nitrate Solution ◽  
Diffusion Method ◽  
E Coli ◽  
Vis Spectroscopy ◽  
Chitosan Composite

<pre><span lang="EN-GB">Bacterial cellulose (C) and its composites were synthesized from rice waste water<strong> </strong>with addition of glycerol (G) and chitosan (Ch).</span><strong></strong><span lang="EN-GB">Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial  cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against <em>S. aureus</em>, <em>E.</em> <em>coli</em>,  and yeast <em>C. albicans</em> has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against <em>S</em>. <em>aureus</em>, <em>E. coli</em>, and <em>C. albicans</em> by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited  the silver nanoparticles against of <em>S. aureus</em>, <em>E. coli</em>,  and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the <em>Staphylococcus aureus</em> ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the <em>E. coli</em> ATCC 25922 and the yeast <em>Candida albicans</em> ATCC 10231.</span></pre>

BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Eli Rohaeti
Keyword(s):  
Waste Water ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Nitrate Solution ◽  
Diffusion Method ◽  
E Coli ◽  
Vis Spectroscopy ◽  
Chitosan Composite

<pre><span lang="EN-GB">Bacterial cellulose (C) and its composites were synthesized from rice waste water<strong> </strong>with addition of glycerol (G) and chitosan (Ch).</span><strong></strong><span lang="EN-GB">Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial  cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against <em>S. aureus</em>, <em>E.</em> <em>coli</em>,  and yeast <em>C. albicans</em> has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against <em>S</em>. <em>aureus</em>, <em>E. coli</em>, and <em>C. albicans</em> by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited  the silver nanoparticles against of <em>S. aureus</em>, <em>E. coli</em>,  and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the <em>Staphylococcus aureus</em> ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the <em>E. coli</em> ATCC 25922 and the yeast <em>Candida albicans</em> ATCC 10231.</span></pre>

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