Uji Aktivitas Antibakteri Ekstrak Sarang Lebah Hutan (Apis Dorsata) Terhadap Pertumbuhan Staphylococcus aureus, Escherichia coli Dan Pseudomonas aeruginosa

Ika Prestianti; Maswati Baharuddin; Sappewali Sappewali

doi:10.20961/alchemy.14.1.13028.179-194

Uji Aktivitas Antibakteri Ekstrak Sarang Lebah Hutan (Apis Dorsata) Terhadap Pertumbuhan Staphylococcus aureus, Escherichia coli Dan Pseudomonas aeruginosa

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.14.1.13028.179-194 ◽

2018 ◽

Vol 14 (1) ◽

pp. 179

Author(s):

Ika Prestianti ◽

Maswati Baharuddin ◽

Sappewali Sappewali

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Apis Dorsata ◽

Soaking Time ◽

E Coli ◽

Extract Concentration ◽

X 24 ◽

Paper Disc

Penyakit infeksi akibat bakteri merupakan masalah serius dalam kesehatan. Antibakteri alami yang dapat digunakan untuk menghambat pertumbuhan bakteri yaitu sarang lebah hutan (Apis dorsata) (propolis, kantong madu, kantong telur dan kantong pollen) yang mengandung senyawa metabolit sekunder seperti flavonoid, tanin dan asam fenolat.Tujuan dari penelitian ini adalah megetahui aktivitas antibakteri ekstrak sarang lebah hutan dari setiap pelarut yang digunakan dan untuk mengetahui pengaruh konsentrasi ekstrak sarang lebah hutan terhadap pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Pseudomonas aeruginosa. Pengujian antibakteri dilakukan dengan metode difusi kertas cakram dengan lama perendaman 1 jam kemudian diinkubasi selama 3 x 24 jam. Hasil penelitian menunjukkan bahwa aktivitas antibakteri ekstrak metanol sarang lebah memiliki aktivitas tertinggi pada bakteri E. coli yaitu 3,8 mm pada konsentrasi 8%, ekstrak etil asetat sarang lebah pada bakteri S. a ureus yaitu 3,72 mm pada konsentrasi 8% dan ekstrak n-heksan sarang lebah pada bakteri E. coli yaitu 16,1 mm pada konsentrasi 8%. Pengaruh konsentrasi ekstrak yaitu semakin tinggi konsentrasi ekstrak maka semakin besar pula daya hambat ekstrak terhadap pertumbuhan bakteri S. aureus, E. coli dan P. aeruginosa.Antibacterial Evaluation of Extract Beehive (Apis dorsata) on Growth Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Bacterial infectious diseases are serious health problems. One of natural antibacterial that can be used to inhibit bacterial growth is honeycomb (propolis, honey bag, egg bag and pollen bag) containing secondary metabolite compounds such as flavonoids, tannins and phenolic acids. The purpose of this research is to know the antibacterial activity of honeycomb extract from each solvent used and to know the effect of honeycomb extract concentration on the growth of S. aureus, E. coli and P. aeruginosa bacteria. Antibacterial testing was performed by the method of paper disc diffusion with soaking time 1 hour then incubated for 3 x 24 hours. The results showed that antibacterial activity of methanol honeycomb extract had the highest activity in E. coli bacteria i.e., 3.8 mm at 8% concentration, ethyl acetate honey extract on S. aureus bacteria ie 3.72 mm at 8% concentration and n extract n-hexan honeycomb in E. coli bacteria that is 16,1 mm at 8% concentration. The higher extract concentration the greater the inhibitory activity of extract on the growth of S. aureus, E. coli and P. aeruginosa bacteria.

Uji Aktivitas Antibakteri Ekstrak Sarang Lebah Hutan (Apis Dorsata) terhadap Pertumbuhan Staphylococa aureus, Escherichia coli dan Pseudomonas aeruginosa

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.14.1.13028.179-189 ◽

2018 ◽

Vol 14 (1) ◽

pp. 179

Author(s):

Ika Prestianti ◽

Maswati Baharuddin ◽

Sappewali Sappewali

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Apis Dorsata ◽

Soaking Time ◽

E Coli ◽

Extract Concentration ◽

Inhibitory Power ◽

X 24

Penyakit infeksi akibat bakteri merupakan masalah serius dalam kesehatan.Antibakteri alami yang dapat digunakan untuk menghambat pertumbuhan bakteri yaitu sarang lebah hutan (Apis dorsata) (propolis, kantong madu, kantong telur dan kantong pollen) yang mengandung senyawa metabolit sekunder seperti flavonoid, tanin dan asam fenolat.Tujuan dari penelitian ini adalah megetahui aktivitas antibakteri ekstrak sarang lebah hutan dari setiap pelarut yang digunakan dan untuk mengetahui pengaruh konsentrasi ekstrak sarang lebah hutan terhadap pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Pseudomonas aeruginosa. Pengujian antibakteri dilakukan dengan metode difusi kertas cakram dengan lama perendaman 1 jam kemudian diinkubasi selama 3 x 24 jam. Hasil penelitian menunjukkan bahwa aktivitas antibakteri ekstrak metanol sarang lebah memiliki aktivitas tertinggi pada bakteri E. coli yaitu 3,8 mm pada konsentrasi 8%, ekstrak etil asetat sarang lebah pada bakteri S. aureus yaitu 3,72 mm pada konsentrasi 8% dan ekstrak n-heksan sarang lebah pada bakteri E. coli yaitu 16,1 mm pada konsentrasi 8%.Pengaruh konsentrasi ekstrak yaitu semakin tinggi konsentrasi maka semakin besar pula daya hambat ekstrak terhadap pertumbuhan bakteri S. aureus, E. coli dan P. aeruginosa.Antibacterial Evaluation of Extract Beehive (Apis dorsata) on Growth Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Bacterial infectious diseases are serious health problems. A natural antibacterial that can be used to inhibit bacterial growth of honeycomb (propolis, honey bag, egg bag and pollen bag) containing secondary metabolite compounds such as flavonoids, tannins and phenolic acids. The purpose of this research is to know the antibacterial activity of honeycomb extract from each solvent used and to know the effect of honeycomb extract concentration on the growth of S. aureus, E. coli and P. aeruginosa bacteria. Antibacterial testing was performed by the method of paper disc diffusion with soaking time 1 hour then incubated for 3 x 24 hours. The results showed that antibacterial activity of methanol honeycomb extract had the highest activity in E. coli bacteria ie 3.8 mm at 8% concentration, ethyl acetate honey extract on S. aureus bacteria ie 3.72 mm at 8% concentration and n extract n-hexan honeycomb in E. coli bacteria that is 16,1 mm at 8% concentration. The effect of extract concentration is the higher the concentration the greater the inhibitory power of extract on the growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa bacteria.

Uji Aktivitas Antibakteri Ekstrak Sarang Lebah Hutan (Apis dorsata) terhadap Pertumbuhan Staphylococcus aureus, Escherichia coli dan Pseudomonas aeruginosa

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.14.2.13028.314-322 ◽

2018 ◽

Vol 14 (2) ◽

pp. 313

Author(s):

Ika Prestianti ◽

Maswati Baharuddin ◽

Sappewali Sappewali

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Apis Dorsata ◽

Bacteria Growth ◽

E Coli ◽

Extract Concentration ◽

Inhibitory Power ◽

X 24

Penyakit infeksi akibat bakteri merupakan masalah serius dalam kesehatan. Antibakteri alami yang dapat digunakan untuk menghambat pertumbuhan bakteri yaitu sarang lebah hutan (Apis dorsata) terdiri propolis, kantong madu, kantong telur dan kantong pollen yang mengandung senyawa metabolit sekunder seperti flavonoid, tanin dan asam fenolat. Tujuan dari penelitian ini adalah megetahui aktivitas antibakteri ekstrak sarang lebah hutan dari setiap pelarut yang digunakan dan untuk mengetahui pengaruh konsentrasi ekstrak sarang lebah hutan terhadap pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Pseudomonas aeruginosa. Pengujian antibakteri dilakukan dengan metode difusi kertas cakram dengan lama perendaman 1 jam kemudian diinkubasi selama 3 x 24 jam. Hasil penelitian menunjukkan bahwa aktivitas antibakteri ekstrak metanol sarang lebah memiliki aktivitas tertinggi pada bakteri E. coli yaitu 3,8 mm pada konsentrasi 8%, ekstrak etil asetat sarang lebah pada bakteri S. aureus yaitu 3,72 mm pada konsentrasi 8% dan ekstrak n-heksan sarang lebah pada bakteri E. coli yaitu 16,1 mm pada konsentrasi 8%. Pengaruh konsentrasi ekstrak yaitu semakin tinggi konsentrasi maka semakin besar pula daya hambat ekstrak terhadap pertumbuhan bakteri S. aureus, E. coli dan P. aeruginosa.Antibacterial Evaluation of Extract Beehive (Apis dorsata) against Growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Bacterial infectious diseases are serious health problems. A natural antibacterial that can be used to inhibit bacterial growth of honeycomb (propolis, honey bag, egg bag and pollen bag) containing secondary metabolite compounds such as flavonoids, tannins and phenolic acids. The purpose of this research is to know the antibacterial activity of honeycomb extract from each solvent used and to know the effect of honeycomb extract concentration against the bacteria growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Antibacterial testing was performed by the method of paper disc diffusion with soaking time 1 hour followed by incubation for 3 x 24 hours. The results show that antibacterial activity of methanol honeycomb extract had the highest activity in E. coli bacteria of 3.8 mm at 8% concentration, followed by those of ethyl acetate honeycomb extract and n-hexan honeycomb extract which have antibacterial activity of 3.72 mm and 16,1 mm at 8% concentration against S. aureus and E. coli, respectively. The effect of extract concentration is the higher the concentration the greater the inhibitory power of extract against the bacteria growth of S. aureus, E. coli and P. aeruginosa.

Uji Daya Hambat Ekstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

Jurnal MIPA ◽

10.35799/jm.3.2.2014.5989 ◽

2014 ◽

Vol 3 (2) ◽

pp. 129

Author(s):

Megawati S. Saroinsong ◽

Febby E. F. Kandou ◽

Adelfia Papu ◽

Marina F. O. Singkoh

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methanol Extract ◽

Inhibition Zone ◽

E Coli ◽

Activity Test ◽

And Staphylococcus Aureus ◽

Paper Disc ◽

Coli Growth

Penelitian ini bertujuan untuk menguji daya hambat dari ekstrak metanol beberapa jenis porifera terhadap pertumbuhan bakteri E. coli dan S. aureus. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer, yaitu dilakukan dengan mengukur zona hambat di sekeliling cakram kertas. Ekstrak Haliclona sp dapat menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan hasil pengukuran diameter zona hambat 13.50 mm, 20.67 mm dan 27.33 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat 10.08 mm, 12.83 mm dan 14.17 mm. Daya hambat ekstrak Agelas sp terhadap S. aureus menunjukkan diameter zona hambat 8.33 mm dan hanya menunjukkan pada konsentrasi ekstrak 90%, sementara daya hambat ekstrak Agelas sp pada konsentrasi 30%, 60% dan 90% terhadap E. coli menunjukkan diameter zona hambat sebesar 7.67 mm, 10.17 mm dan 14.17 mm. Daya hambat Spheciospongia sp terhadap S. aureus dan E. coli hanya terlihat pada konsentrasi ekstrak sebesar 90% dengan diameter zona hambat adalah 8.42 mm dan 8.75 mm. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa ekstrak Haliclona sp memiliki potensi aktivitas antibakteri yang dapat digunakan sebagai bahan dasar pembuatan obat antibiotik.This research aimed to test the inhibition capabilities of methanol extract from several kinds of Porifera on Escherichia coli and Staphylococcus aureus growth. The antibacterial activity test using the Kirby-Bauer method, which delivered by measuring the inhibition zone around paper disc. The extract of Haliclona sp can inhibit the S. aureus growth at 30%, 60% and 90% of extracts concentration with the measurement of inhibition zone diameters are 13.50 mm, 20.67 mm and 27.33 mm; also inhibit the E. coli growth with inhibition zone diameters are 10.08 mm, 12.83 mm and 14.17 mm. The inhibition capability of Agelas sp extract on S. aureus shows that the inhibition zone diameters is 8.83 mm and only appear at 90% of extracts concentration, meanwhile the inhibition capability of Agelas sp extract at concentration 30%, 60% and 90% on E. coli shows diameters of inhibition zone are 7.67 mm, 10.17 mm and 14.17 mm. The inhibition capability of Spheciospongia sp on S. aureus and E. coli only occurred at 90% of extracts concentration with inhibition zone diameters 8.42 mm and 8.75 mm. Based on the results, it can be assumed that extracts of Haliclona sp has a potential antibacterial activity that can be used as a basic ingredients for antibiotic medicine.

Evaluation of Antibacterial Activity of Three Quaternary Ammonium Disinfectants on Different Germs Isolated from the Hospital Environment

BioMed Research International ◽

10.1155/2020/6509740 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Amal Ramzi ◽

Bouchra Oumokhtar ◽

Yassine Ez zoubi ◽

Touria Filali Mouatassem ◽

Moussa Benboubker ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Quaternary Ammonium ◽

Inhibitory Concentration ◽

Hospital Environment ◽

Gram Positive ◽

Gram Negative ◽

E Coli

Background. The microbiological risk of the hospital environment, including inert surfaces, medical devices, and equipment, represents a real problem. Objective. This study is aimed at demonstrating and assessing the antibacterial activity of three synthetic disinfectants classified as quaternary ammoniums on different bacterial strains (Gram-negative and Gram-positive like Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Staphylococcus aureus) isolated from the hospital environment. The reference strains included Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, and Pseudomonas aeruginosa ATCC 27853 used as negative control strains. Method. Three quaternary ammonium disinfectants were tested: DDN9® (0.5%) which contains didecylmethylpolyoxyethylammonium propionate as an active substance, spray (0.4%) containing quaternary ammonium compounds, and Phagosurf ND® (0.4%) with didecyldimethylammonium chloride. Their effect was evaluated using the disk diffusion technique and the broth dilution methods, allowing the Minimum Inhibitory Concentration (MIC) and then the Minimum Bactericidal Concentration (MBC). Result. Only the growth of Gram-positive bacteria and some strains of Gram-negative bacteria were inhibited by the three synthetic disinfectants. NDD9® demonstrated an antibacterial effect only against the Gram-positive strains (S. aureus and S. aureus ATCC 29213) with a MIC of 0.25 mg/ml. The disinfectant spray showed effect against all four strains including E. coli (9), S. aureus, E. coli ATCC 25922, and P. aeruginosa ATCC 27853 with an inhibitory concentration of 4 mg/ml, while the growth of S. aureus ATCC 29213 was inhibited at 2 mg/ml. The third disinfectant, Phagosurf ND®, inhibited only the growth of S. aureus ATCC 29213 at a MIC of 4 mg/ml. Conclusion. This study is the first here in Morocco to evaluate the bacterial activity of products intended for the control of the healthcare environment. The results obtained on the three disinfectants tested reveal an ineffectiveness against some isolated strains from the hospital environment.

KHẢO SÁT ĐIỀU KIỆN NUÔI CẤY ĐỂ NÂNG CAO KHẢ NĂNG KHÁNG KHUẨN CỦA CÁC CHỦNG Streptomyces sp.

Tạp chí Khoa học và công nghệ nông nghiệp, Trường Đại học Nông Lâm Huế ◽

10.46826/huaf-jasat.v5n1y2021.459 ◽

2021 ◽

Vol 5 (1) ◽

pp. 2227-2236

Author(s):

Nguyễn Thị Thủy Tiên ◽

Lê Thị Thu Hiền ◽

Trần Thị Lệ Ngân ◽

Trần Văn Trung ◽

Nguyễn Thị Thúc ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

16S Rrna ◽

Bacillus Cereus ◽

Salmonella Typhi ◽

Streptomyces Sp ◽

International Streptomyces Project ◽

E Coli

Streptomyces là những vi sinh vật có khả năng sinh tổng hợp các chất kháng sinh. Nghiên cứu này nhằm sàng lọc và chọn chủng có khả năng kháng khuẩn cao nhất từ 59 chủng Streptomyces có nguồn gốc từ đất đã được cung cấp dựa trên phương pháp cấy vạch vuông góc đối với 5 vi khuẩn gây bệnh chỉ thị, bao gồm Bacillus cereus, Escherichia coli, Salmonella typhi, Staphylococcus aureus và Pseudomonas aeruginosa. Mười lăm chủng thể hiện hoạt tính kháng khuẩn ở các mức độ khác nhau, các chủng còn lại không thể hiện khả năng kháng. Chủng có khả năng kháng khuẩn cao nhất là HĐM3.2, kháng 4/5 vi khuẩn chỉ thị đã sử dụng, gồm B. cerius, S. aureus, S. typhi và E. coli với kích thước vùng kháng đạt 9,5, 10,5, 16,5 và 14,5 mm. Chủng HĐM3.2 được xác định là chủng Streptomyces sp. dựa trên cây phát sinh loài đã xây dựng của trình tự gene 16S rRNA. Điều kiện nuôi cấy để chủng HĐM3.2 thể hiện hoạt tính kháng khuẩn cao nhất đã được khảo sát dựa trên phương pháp khuếch tán qua giếng thạch. Môi trường International Streptomyces Project 2 có pH 8, nhiệt độ nuôi cấy 28oC là điều kiện thích hợp để chủng Streptomyces sp. HĐM3.2 tạo ra vùng ức chế các loại vi khuẩn B. cereus, E. coli, S. typhi, S. aureus cao nhất. ABSTRACT Streptomyces is the microorganism that has capable of producing antibiotics. The current study aimed to screen and to select a strain that had the highest antibacterial activity from 59 available soil-derived Streptomyces strains based on the perpendicular culture method on 5 indicator pathogenic bacteria, including Bacillus cereus, Escherichia coli, Salmonella typhi, Staphylococcus aureus và Pseudomonas aeruginosa. Fourteen strains exhibited their antibacterial activity at various levels, the remaining did not have that activity. The strain that had the highest antibacterial ability was HĐM3.2 against 4/5 indicator microorganisms, including B. cerius, S. aureus, S. typhi and E. coli with inhibitory areas were 9.5, 10.5, 16.5, and 14.5 mm respectively. Strain HĐM3.2 was identified as strain Streptomyces sp. based on a phylogenetic tree built on the 16S rRNA gene sequences. Suitable conditions that made the strain HĐM3.2 showed the highest antibacterial activity were investigated based on agar well diffusion assay. The medium of International Streptomyces Project 2 with pH 8, the ambient temperature at 28oC were suitable conditions for Streptomyces sp. HĐM3.2 produced the highest inhibitory areas against B. cereus, E. coli, S. typhi, S. aureus.

Chemical Profile, Antibacterial Activity and Antibiotic-Modulating Effect of the Hexanic Zea Mays L. Silk Extract (Poaceae)

Antibiotics ◽

10.3390/antibiotics8010022 ◽

2019 ◽

Vol 8 (1) ◽

pp. 22

Author(s):

Ana Carvalho ◽

Cleciana Cruz ◽

Cicero Freitas ◽

José Aguiar ◽

Paula Nunes ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Zea Mays ◽

Antibacterial Activity ◽

Inhibitory Concentration ◽

Synergistic Effects ◽

Chemical Constituents ◽

Chemical Profile ◽

E Coli

The present study aimed to determine the chemical profile and to evaluate the antibacterial activity and antibiotic-modulating action of the hexanic Zea mays silk extract in association with aminoglycosides. Standard Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 strains, as well as multi-resistant Escherichia coli 27, Staphylococcus aureus 35, and Pseudomonas aeruginosa 31 strains, were used in this study. Phytochemical prospection revealed the presence of the following secondary metabolites: tannins, flavones, flavonoids, and xanthones, with the main chemical constituents being identified in plant extracts obtained with apolar organic solvents such as hexane. The extract presented a minimum inhibitory concentration (MIC) ≥1024 μg/mL against all the tested strains. The association of the extract with aminoglycoside antibiotics showed significant synergistic effects against Staphylococcus aureus and Pseudomonas aeruginosa, except for amikacin, which was antagonized by the extract against E. coli. These results indicate the Zea mays silk presents bioactive compounds with antibiotic-modulating properties. However, further research is required to characterize the effects of isolated compounds and determine their potential for drug development.

Antibacterial and Antifungal Activity of the Extracts of Different Parts of Avicennia marina (Forssk.) Vierh

Plants ◽

10.3390/plants10020252 ◽

2021 ◽

Vol 10 (2) ◽

pp. 252

Author(s):

Mohammad K. Okla ◽

Abdulrahman A. Alatar ◽

Saud S. Al-amri ◽

Walid H. Soufan ◽

Altaf Ahmad ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Antibacterial Activity ◽

Antifungal Activity ◽

Aspergillus Fumigatus ◽

Ethanolic Extract ◽

Avicennia Marina ◽

E Coli

Increased problems associated with side effects and bacterial resistance of chemical drugs has prompted the research focus on herbal medicines in the past few decades. In the present investigation, the antimicrobial activity of the various parts of Avicennia marina (AM), a mangrove plant, has been evaluated. The plants were collected from the Jazan area of the Kingdom of Saudi Arabia. Primary extracts of roots, stem, leaves, fruits, and seeds were made in ethanol and fractioned in ethanol, ethyl acetate, petroleum ether, chloroform, and water. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the extracts were determined against Bacillussubtilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. It has been observed that the chloroform extract of roots of the AM exhibited inhibitory effects against both S. aureus (MIC = 1.5 ± 0.03 mg/mL) and E. coli (MIC = 1.7 ± 0.01 mg/mL). The ethanolic extract of the AM roots has shown antibacterial activity against Pseudomonas aeruginosa (MIC = 10.8 ± 0.78 mg/mL), Bacillussubtilis (MIC = 6.1 ± 0.27 mg/mL), Staphylococcus aureus (MIC = 2.3 ± 0.08 mg/mL), and Escherichia coli (MIC = 6.3 ± 0.28 mg/mL). The leaf extract of the AM in ethyl acetate showed antibacterial activity against S. aureus and E. coli. Antifungal activity of these extracts was also investigated against Aspergillus fumigatus and Candida albicans. Ethanolic extract of roots and seeds of the AM has shown antifungal activity against Aspergillus fumigatus when applied individually. Ethanolic extract of the AM fruits has shown an inhibitory effect on the growth of Aspergillus fumigatus and Candida albicans. It is suggested that the plant extracts of AM have tremendous antimicrobial activity against a group of microbes, and this effect depends on both the plant part and the solvent used for extraction. Therefore, this plant can be considered to treat various diseases caused by antibiotic-resistant bacteria.

Bioactive compounds isolated from submerged fermentations of the Chilean fungus Stereum rameale

Zeitschrift für Naturforschung C ◽

10.1515/znc-2015-5005 ◽

2015 ◽

Vol 70 (3-4) ◽

pp. 97-102 ◽

Cited By ~ 7

Author(s):

Pedro Aqueveque ◽

Carlos Leonardo Céspedes ◽

José Becerra ◽

Marcelo Dávila ◽

Olov Sterner

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Bioactive Metabolites ◽

Gram Negative Bacteria ◽

Gram Negative ◽

E Coli ◽

Gram Positive Bacteria ◽

Bioassay Guided Fractionation

Abstract Liquid fermentations of the fungus Stereum rameale (N° 2511) yielded extracts with antibacterial activity. The antibacterial activity reached its peak after 216 h of stirring. Bioassay-guided fractionation methods were employed for the isolation of the bioactive metabolites. Three known compounds were identified: MS-3 (1), vibralactone (2) and vibralactone B (3). The three compounds showed antibacterial activity as a function of their concentration. Minimal bactericidal concentrations (MBC) of compound 1 against Gram-positive bacteria were as follows: Bacillus cereus (50 μg/mL), Bacillus subtilis (10 μg/mL) and Staphylococcus aureus (100 μg/mL). Compounds 2 and 3 were active only against Gram-negative bacteria. The MBC of compound 2 against Escherichia coli was 200 μg/mL. Compound 3 inhibited significantly the growth of E. coli and Pseudomonas aeruginosa, with MBC values of 50 and 100 μg/mL, respectively.

IDENTIFIKASI FITOKIMIA DAN UJI DAYA HAMBAT DARI EKSTRAK ETANOL TANGKAI BUAH PINANG YAKI (Areca vestiaria giseke) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli, DAN Pseudomonas aeruginosa

PHARMACON ◽

10.35799/pha.8.2019.29373 ◽

2019 ◽

Vol 8 (4) ◽

pp. 936

Author(s):

Danni U. W. Redwik ◽

Herny E. I. Simbala ◽

Hosea Jaya Edi

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Diffusion Method ◽

Areca Nut ◽

Phytochemical Content ◽

E Coli ◽

The North ◽

Ethanol Extracts

ABSTRACT Areca nut (Areca vestiaria Giseke), which is a kind of wild palm, is a multi-functional plant. The North Sulawesi people empirically use this plant to cure various diseases. This study aims to determine the phytochemical content and antibacterial activity of ethanol extracts of areca nut against the inhibitory growth of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa using 5 concentrations namely 1%, 2%, 3%, 4% and 5%. Extraction was done by maceration using 96% of ethanol. Antibacterial activity testing was using paper disc diffusion method (Kirby and Bauer diffusion). The results of this study indicate that the ethanol extracts of areca nut stem contains flavonoids, saponins, triterpenoids that have the potential to be antibacterial and has antibacterial strength against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, at concentrations of 4% and 5%, which are concentrations which are included in the group that categorize as strong to inhibit the bacteria E. coli and P. aeruginosa, while the extract concentration of 2%, 3%, 4%, and 5% which are included in the group that categorize as strong to inhibit the S. aureus bacteria. Keywords: Agar diffusion method, Antibacterial, Areca nut, Phytochemical. ABSTRAK Pinang Yaki (Areca vestiaria Giseke) yang merupakan sejenis palem liar, merupakan tanaman yang multi fungsi. Masyarakat Sulawesi Utara secara empiris menggunakan tanaman ini untuk menyembuhkan berbagai macam penyakit.. Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan aktivitas antibakteri dari ekstrak etanol tangkai buah pinang yaki terhadap daya hambat pertumbuhan bakteri Escherichia coli, Staphylococcus aureus, dan Pseudomonas aeruginosa menggunakan 5 konsentrasi yakni 1%, 2%, 3%, 4%, dan 5%. Ekstraksi dilakukan dengan cara maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri menggunakan metode difusi dengan cakram kertas (difusi Kirby dan Bauer). Hasil penelitian ini menunjukkan bahwa ekstrak etanol tangkai buah Pinang Yaki memiliki kandungan flavonoid, saponin, triterpenoid yang berpotensi sebagai antibakteri dan memiliki kekuatan antibakteri terhadap Escherichia coli, Staphylococcus aureus, dan Pseudomonas aeruginosa, pada konsentrasi 4% dan 5% merupakan konsentrasi yang termasuk dalam golongan kuat untuk menghambat bakteri E. coli dan P. aeruginosa, sedangkan pada konsentrasi ekstrak 2%, 3%, 4%, dan 5% termasuk dalam golongan kuat menghambat bakteri S. aureus. Kata kunci: Pinang Yaki, Fitokimia, Antibakteri,metode difusi agar

Phytochemical Analysis and Antibacterial Activity of Moringa oleifera Leaves Extracts against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2021/v7i130166 ◽

2021 ◽

pp. 34-43

Author(s):

Sheriffdeen Bale Issa ◽

Muhyiddeen Muazu ◽

Isma’il Rabi’u

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Moringa Oleifera ◽

Ethanol Extract ◽

Phytochemical Analysis ◽

Aqueous Extracts ◽

E Coli ◽

Ethanol Extracts

This study tests the antibacterial activities of Moringa oleifera leaf extract against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, obtained from Microbiology laboratory, Al-Hikmah University Ilorin. Phytochemical analysis reveals the presence of tannins in all the three extracts (Aqueous, ethanolic and N-hexane), while. flavonoids, alkaloids and Saponins were only present in the Aqueous and ethanolic extracts. The leaves extracts were screened for antibacterial activity by agar well diffusion method, employing five different extracts concentrations (100mg/ml, 80mg/ml, 60mg/ml, 40mg/ml and 20mg/ml). The aqueous extracts had a mean activity of 11.50±0.70mm, 7.50±0.70mm and 8.5±0.70mm for S. aureus, E. coli and P. aeruginosa respectively. The ethanol extracts had a mean activity of 12.00±1.41mm, 10.00±1.41mm and 8.00±1.21mm for S. aureus, E. coli and P. aeruginosa respectively, while the N-hexane extracts exert no any activity. The antibiotics discs exert more inhibitory effect compared to the extract. The MIC for the aqueous extracts are at a concentrations of 60mg/ml for S. aureus, 80mg/ml for P. aeruginosa and 80mg/ml for E. coli. While that of the ethanol extract of the leaves are at a concentration of 60mg/ml for S. aureus, 80mg/ml for E. coli and 100mg/ml for P. aeruginosa. The MBC for the aqueous Extract of the leaves are at a concentration of 80mg/ml for S. aureus, 100mg/ml for P. aeruginosa and 100mg/ml for E. coli, while that of the ethanol extract are at a concentration of 80mg/ml for S. aureus and 100mg/ml for E. coli), while no any MBC was recorded for P. aeruginosa. The antibacterial activity tests indicate that the ethanol extract was more active. Among the three isolates, S. aureus is more sensitive to the aqueous and ethanol extracts. The activity exhibited by the extracts may be related to the presence of a number of Phytoconstituents.