scholarly journals Uji Aktivitas Antioksidan N-Metil Kitosan Berkelarutan Tinggi

2018 ◽  
Vol 14 (1) ◽  
pp. 124
Author(s):  
Mardiyah Kurniasih ◽  
Purwati Purwati ◽  
Ratna Stia Dewi ◽  
Susi Fatimah
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
High Solubility ◽  
Chitosan Concentration ◽  
Ferric Thiocyanate ◽  
Activity Test ◽  
Dpph Method

<p>Telah dilakukan uji aktivitas antioksidan dari N-metil kitosan yang memiliki kelarutan tinggi. Uji aktivitas antioksidan dilakukan dengan menggunakan metode DPPH (1,1-diphenyl-2-pikrilhidrazil) dan metode FTC (<em>ferric thiocyanate</em>). Optimasi sintesis dilakukan untuk memperoleh N-metil kitosan dengan kelarutan tinggi. Hasil penelitian menunjukkan bahwa N-metil kitosan yang berkelarutan paling tinggi adalah N-metil kitosan yang disintesis dengan 1,7 mL formaldehid 10 %. Berdasarkan hasil uji aktivitas antioksidan menggunakan metode DPPH dapat diketahui bahwa N-metil kitosan merupakan antioksidan sedang dengan nilai IC<sub>50</sub> sebesar 145,398 ppm. Hasil pengujian antioksidan metode FTC menunjukkan bahwa N-metil kitosan memiliki kemampuan menghambat terbentuknya senyawa radikal bebas yang disebabkan oleh oksidasi asam lemak.</p><p><strong>An Antioxidant Activity Test of High Solubility N-Methyl Chitosan</strong><strong>.</strong> An antioxidant activity test of high solubility N-methyl chitosan has been performed. The antioxidant activity test was performed using DPPH (1,1-diphenyl-2-pikrilhidrazil) and FTC method (ferric thiocyanate). The synthesis optimization was performed to obtain high N-methyl chitosan solubility. The results showed that N-methyl chitosan with the highest solubility in 1 % acetic acid solvent was N-methyl chitosan which was synthesized with 1.7 mL formaldehyde 10 %. Based on the results of antioxant activity test using DPPH method can be seen that N-methyl chitosan is a medium antioxidant with IC<sub>50</sub> value of 145.398 ppm. The result of antioxidant test of FTC method showed that N-methyl chitosan has antioxidant activity with tendency to increase along with the increase of N-methyl chitosan concentration.</p>

scholarly journals Uji Aktivitas Antioksidan N-Metil Kitosan Berkelarutan Tinggi

2018 ◽  
Vol 14 (1) ◽  
pp. 107
Author(s):  
Mardiyah Kurniasih ◽  
Purwati Purwati ◽  
Ratna Stia Dewi ◽  
Susi Fatimah
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
High Solubility ◽  
Chitosan Concentration ◽  
Ferric Thiocyanate ◽  
Activity Test ◽  
Dpph Method

<p>Telah dilakukan uji aktivitas antioksidan dari N-metil kitosan yang memiliki kelarutan tinggi. Uji aktivitas antioksidan dilakukan dengan menggunakan metode DPPH (1,1-diphenyl-2-pikrilhidrazil) dan metode FTC (<em>ferric thiocyanate</em>). Optimasi sintesis dilakukan untuk memperoleh N-metil kitosan dengan kelarutan tinggi. Hasil penelitian menunjukkan bahwa N-metil kitosan yang berkelarutan paling tinggi adalah N-metil kitosan yang disintesis dengan 1,7 mL formaldehid 10%. Berdasarkan hasil uji aktivitas antioksidan menggunakan metode DPPH dapat diketahui bahwa N-metil kitosan merupakan antioksidan sedang dengan nilai IC<sub>50</sub> sebesar 145,398 ppm. Hasil pengujian antioksidan metode FTC menunjukkan bahwa N-metil kitosan memiliki kemampuan menghambat terbentuknya senyawa radikal bebas yang disebabkan oleh oksidasi asam lemak.</p><p><strong>An Antioxidant Activity Test of High Solubility N-Methyl Chitosan</strong><strong>.</strong> An antioxidant activity test of high solubility N-methyl chitosan has been performed. The antioxidant activity test was performed using DPPH (1,1-diphenyl-2-pikrilhidrazil) and FTC method (ferric thiocyanate). The synthesis optimization was performed to obtain high N-methyl chitosan solubility. The results showed that N-methyl chitosan with the highest solubility in 1% acetic acid solvent was N-methyl chitosan which was synthesized with 1.7 mL formaldehyde 10%. Based on the results of antioxant activity test using DPPH method can be seen that N-methyl chitosan is a medium antioxidant with IC<sub>50</sub> value of 145.398 ppm. The result of antioxidant test of FTC method showed that N-methyl chitosan has antioxidant activity with tendency to increase along with the increase of N-methyl chitosan concentration.</p>

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SENYAWA PIRAZOLIN 3-(2- METOKSI-FENIL)-5-NAFTALEN-1-IL-4,5-DIHIDRO-1H-PIRAZOL

2018 ◽  
Vol 8 (2) ◽  
pp. 53-58
Author(s):  
Nurlaili ◽  
Jasril ◽  
Hilwan Yuda Teruna
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
Microwave Irradiation ◽  
Biological Activities ◽  
Spectroscopy Data ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Anti Inflammatory

Pyrazoline compounds are reported to have many useful biological activities such as antimicrobials, antihyperglycemia, antioxidants, anti-inflammatory, anticancer and anticonvulsants. The pyrazoline analogues of 3- (2-methoxy-phenyl) -5-naphthalene-1-yl-4,5-dihydro-1H-pyrazol were synthesized usingglacial acetic acid as catalyst and assisted by microwave irradiation. The structures of the compounds were obtainedby UV, IR and HRMS spectroscopy data. Antioxidant activity test using DPPH method showed that pyrazoline compound had IC50 value of 27.41 μg/mL.

Ekstraksi Protein dari Rhodophyta dan Chlorophyta Dari Perairan Pulau Pari Sebagai Alternatif Antioksidant

2020 ◽  
Vol 1 (1) ◽  
pp. 24-31
Author(s):  
Elvi Kustiyah ◽  
Bungaran Saingin ◽  
Hernowo Widodo ◽  
Viriya Piti
Keyword(s):  
Antioxidant Activity ◽  
Low Temperature ◽  
Protein Content ◽  
Green Algae ◽  
Red Algae ◽  
Marine Resources ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Lowry Method

 Indonesia has millions island and big part of Indonesia is sea that is rich in marine biological resources and has the potential to be developed and optimized. One of the abundant marine resources in Indonesia is algae. Algae are plant-like protists. Algae have several important compounds, including protein, carbohydrates, fats, minerals and other useful elements. Proteins contained in algae have the potential to be used as antioxidants. In this study, the levels of protein in red and green algae were tested by using the lowry method and testing the antioxidant activity of red and green algae extracts using the Diphenylpicrylhydrazyl (DPPH) method. Algae extraction was done by maceration, which is soaking the sample in low temperature with phosphate buffer saline (PBS) pH 7. From the extraction results it can be concluded that the red algae (Rhodophyta) has the highest protein content of 5.115 ± 0.126% and the lowest protein content in green algae (Chloropytha) as big as 1.686 ± 0.430%. And from the results of the antioxidant activity test showed that all positive algae showed antioxidant activity but the green algae (Chlorophyta) had the highest antioxidant activity of 71.5946 ± 0.01612% with IC50 value 1.6114.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN TOTAL KANDUNGAN FENOLIK EKSTRAK ETANOL DAUN NANAMUHA (Bridelia monoica Merr)

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 178
Author(s):  
Julia Elsa Lakoro ◽  
Max R. J. Runtuwene ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Total Phenol ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phenol Compounds ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ethanol Extracts

ABSTRACTNanamuha (Bridelia monoica Merr.) is a plant that was used as a cancer and tumor drug by the Sangihe Islands community. This plant contains phenol compounds, flavonoids, alkaloids and tannins. The purpose of this study was to determine the potential of nanamuha leaves that grow in the Sangihe Island to have antioxidant activity and determine the total phenolic ethanol extracts of nanamuha leaf. The extraction method used is maceration with ethanol solvent p.a. The extracts obtained was determined total phenolic and antioxidant activity test using DPPH method. Based on the results obtained, the ethanol extracts of nanamuha leaves has antioxidant activity with an IC50 value of 75,03 µg / mL and the result showed that the total phenol content the ethanol extracts of nanamuha leaves of  53,34 mg / L.  Keywords : Nanamuha leaf, total phenol, antioxidant activity, DPPH  ABSTRAKNanamuha (Bridelia monoica Merr.) merupakan tanaman yang dimanfaatkan sebagai obat kanker dan tumor oleh masyarakat Kepulauan Sangihe. Tanaman ini memiliki kandungan senyawa fenol, flavonoid, alkaloid dan tanin. Tujuan dari penelitian ini adalah unutuk mengetahui potensi dari daun nanamuha yang tumbuh di Kepulaun Sangihe ini memiliki aktivitas antioksidan dan menentukan total fenolik dari ekstrak etanol daun nanamuha. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol p.a. Ekstrak yang diperoleh ditentukan total fenolik dan uji aktivitas antioksidan dengan metode DPPH. Berdasarkan hasil yang didapatkan ekstrak etanol daun nanamuha memiliki aktivitas antioksidan dengan nilai IC50 75,03 µg/mL dan hasil penelitian menunjukan total kandungan fenolik ekstrak etanol daun nanamuha sebesar 53,34 mg/L. Kata kunci : Daun Nanamuha, Total fenol, Aktivitas antioksidan, DPPH

The antioxidant activity test by using DPPH method from the white tea using different solvents

2017 ◽  
Author(s):  
Doddy A. Darmajana ◽  
Firman Hadiansyah ◽  
Dewi Desnilasari
Keyword(s):  
Antioxidant Activity ◽  
White Tea ◽  
Activity Test ◽  
Dpph Method

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

2021 ◽  
Vol 18 (1) ◽  
pp. 60-67
Author(s):  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Sadam Husein ◽  
Titi Pudji Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidant ◽  
Activity Test ◽  
Impaired Liver ◽  
Ic50 Value ◽  
Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

scholarly journals Antioxidant Activity of Dominant Plants Species in Obat Pahit from Lingga Malay Ethnic in Riau Archipelago

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Fitmawati Fitmawati ◽  
Nery Sofiyanti ◽  
Rodesia Mustika Roza ◽  
Isnaini Isnaini ◽  
Yulisa Resti Irawan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Natural Resources ◽  
The Other ◽  
Distilled Water ◽  
Water Solvent ◽  
Activity Test ◽  
Dpph Method ◽  
Riau Archipelago

<p><em>Obat Pahit</em> is a potion that has been long commonly consumed by Lingga Malay society for generations as stamina keeper. The most dominant plants found in the packaging of the Obat Pahit were namely <em>Bauhunia semibifida, Cnestis palala</em> and Penawa Root (3 species). This research aimed to investigate and determine activity of antioxidant contents in <em>Obat Pahit</em> from five Traditional Medicine Practitioners (TMPs) in the district of Lingga. The tested samples were mashed then being soaked into 2 types of solvent: distilled water and methanol, containing HCl 1%. DPPH method was also used in this research. Quantitatively antioxidant activity test of <em>Obat Pahit</em> from the five TMPs by using methanol solvent had extremely highest activity compared to the distilled water solvent. The test, using TLC plate by spraying the extract from three dominant plants with 0.1 mM of DPPH solution, produced a pale-yellow spots at a wavelength of 366 nm. On the other hand, the test using HPLC at wavelengths of 230 nm and 280 nm showed the presence of two dominant secondary metabolites contents: flavonoid and phenolic. IC50 (ppm) of <em>Bauhinia semibifida</em> (6.6247), Penawa Root (5.0124) and <em>Cnestis palala</em> (5.9968) were much lower than IC50 of mangosteen’s rind (41.7675), vitamin C (6.6612) and Stimuno drug (8.333). This antioxidant analysis has not been reported previously. This proof contributed greatly to uncovering potentially native natural resources as an indigenous Indonesian drug which is expected to decrease dependence on imported drugs especially imunomodulator, antihypertensive, antidiabet etc. This research would be beneficial and excellent manifestation for the development of natural antioxidant-based medicines from traditional knowledge of Indonesia’s local ethnicities.</p>

scholarly journals STUDI OPTIMASI EKSTRAKSI KANDUNGAN SENYAWA FENOLIK TOTAL DAN UJI AKTIVITAS ANTIOKSIDAN DARI DAUN MANGGIS (Garcinia mangostana Linn.)

2019 ◽  
Vol 10 (1) ◽  
pp. 36
Author(s):  
Emil Salim ◽  
Yogi Afritunando ◽  
Nindi Antika Febriana ◽  
Mai Efdi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Garcinia Mangostana ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method

This study aims to determine antioxidant activity and total phenolic content of mangosteen leaves in several different solvents. The antioxidant activity test of the extract was determined by the DPPH method and the total phenolic content was determined by the Folin-Ciocalteu method. The phytochemical test of mangosteen leaves showed the presence of phenolic compounds, saponins, triterpenoids and alkaloids. The extraction results increase with increasing water content in methanol, ethanol and acetone. The highest amount of extract was obtained using 50% acetone solvent (34.98 ± 0.17%). Extraction using 100% ethanol showed the highest total phenolic content (614.25 ± 5.58 mg GAE / g extract) and the lowest IC50 value (5.81 mg / L). The results of this study indicate that the use of 50% acetone solvent can optimize the amount of extract and 100% ethanol can optimize the content of total phenolic compounds and antioxidant activity of mangosteen leaves.

scholarly journals AKTIVITAS ANTIOKSIDAN EKSTRAK TUMBUHAN PAKU EKOR KUDA (Equisetum debile L.) TERHADAP PEROKSIDASI LIPID PLASMA DARAH MENCIT (Mus musculus)

2017 ◽  
Vol 4 (1) ◽  
pp. 48
Author(s):  
Riana Dyah Suryaningrum ◽  
Ni Made Puspawati ◽  
Ni Putu Adriani Astiti
Keyword(s):  
Antioxidant Activity ◽  
Statistical Analysis ◽  
Free Radical ◽  
Blood Plasma ◽  
Mus Musculus ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Activity Test ◽  
Dpph Method

The purpose of this research was to study the antioxidant activity from ethanol extract of horstail (Equisetum debile L.) in the free radical scavenging in mice blood plasma. The antioxidant activity test was conducted with the DPPH method and measuring the MDA concentration in mice blood. The antioxidant activity test with the DPPH results showed that the ethanol extract of horstail (Equisetum debile L.) had the antioxidant activity of IC50 which was 1.604 mg/mL or 1,604 ppm. The statistical analysis result of the MDA blood plasma in mice with various doses (125 mg/kgBM, 250 mg/kgBM, 375 mg/kgBM and 500 mg/kgBM) showed that the 500 mg/kgBM dose extract was able to reduce the MDA concentration in mice blood which given the most amount of exercise.

Sign in / Sign up

Export Citation Format

Share Document