scholarly journals Asosiasi Marka Genetik dengan Pertambahan Bobot Badan Sapi Madura di Pamekasan

2017 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Suyadi Suyadi ◽  
Isnaini Isnaini ◽  
Rahayu Rahayu ◽  
Y. Nurpah
Keyword(s):  
Growth Rate ◽  
Blood Sample ◽  
Dna Markers ◽  
Dna Polymorphism ◽  
Dna Marker ◽  
Efficient Tool ◽  
Salting Out ◽  
Key Words Dna ◽  
Pcr Product ◽  
Gh Gene

<p>Characteristic of DNA markers may be able to be used as useful and efficient tool to select merit animal from a population. In order to Madura cattle, growth rate is one of important traits should be considered. The aim of this study was to evaluate the association between the characteristic of DNA marker of candidate gene for growth hormone (GH) and growth rate of Madura cattle. A number of 10 female Madura cattle selected from 40 animals in average of about 18 months old were used as sample. The animal was weighed twice using electronic balance at the interval time of two months. At the simultaneous time to the animal weighing the blood sample was collected via vena jugularis 6 ml of each for DNA source. The blood sample was dropped ito polypropylene tubes containing EDTA for anti coagulant agent. DNA was isolated from leucocyte cells in the blood using salting out as standard method. PCR technique was used for amplifying the DNA using GH primer (forward: 5’-TAGGGAGGTGGAAAATGGA-3’ and reverse: 5’-GACACCTAGACAATGCG-3’). DNA polymorphism of GH gene was detected using RFLP technique by digesting the PCR-product DNA with HaeIII enzyme at position of GG*CC. The results showed that amplified DNA with this primer showed a single band of 450bp. Restriction of DNA with HaeIII enzyme resulted 4 haplotypes of uncut fragment , 2 fragments (2a and 2b), 3 fragments (3a and 3b) and 5 fragments at the position of 125bp, 200bp, 275bp and 450 bp. According to the data analysis, the non significant association was shown between specific genetic polymorphism and growth rate in this cattle.</p><p><em> </em></p><p>Key words: DNA marker, GH gene, growth rate, Madura cattle</p>

scholarly journals Asosiasi Marka Genetik dengan Pertambahan Bobot Badan Sapi Madura di Pamekasan

2017 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Suyadi Suyadi ◽  
Isnaini Isnaini ◽  
Rahayu Rahayu ◽  
Y. Nurpah
Keyword(s):  
Growth Rate ◽  
Blood Sample ◽  
Dna Markers ◽  
Dna Polymorphism ◽  
Dna Marker ◽  
Efficient Tool ◽  
Salting Out ◽  
Key Words Dna ◽  
Pcr Product ◽  
Gh Gene

<p>Characteristic of DNA markers may be able to be used as useful and efficient tool to select merit animal from a population. In order to Madura cattle, growth rate is one of important traits should be considered. The aim of this study was to evaluate the association between the characteristic of DNA marker of candidate gene for growth hormone (GH) and growth rate of Madura cattle. A number of 10 female Madura cattle selected from 40 animals in average of about 18 months old were used as sample. The animal was weighed twice using electronic balance at the interval time of two months. At the simultaneous time to the animal weighing the blood sample was collected via vena jugularis 6 ml of each for DNA source. The blood sample was dropped ito polypropylene tubes containing EDTA for anti coagulant agent. DNA was isolated from leucocyte cells in the blood using salting out as standard method. PCR technique was used for amplifying the DNA using GH primer (forward: 5’-TAGGGAGGTGGAAAATGGA-3’ and reverse: 5’-GACACCTAGACAATGCG-3’). DNA polymorphism of GH gene was detected using RFLP technique by digesting the PCR-product DNA with HaeIII enzyme at position of GG*CC. The results showed that amplified DNA with this primer showed a single band of 450bp. Restriction of DNA with HaeIII enzyme resulted 4 haplotypes of uncut fragment , 2 fragments (2a and 2b), 3 fragments (3a and 3b) and 5 fragments at the position of 125bp, 200bp, 275bp and 450 bp. According to the data analysis, the non significant association was shown between specific genetic polymorphism and growth rate in this cattle.</p><p><em> </em></p><p>Key words: DNA marker, GH gene, growth rate, Madura cattle</p>

scholarly journals Construction of DNA ladder for determination of small size DNA fragments

2021 ◽  
Vol 19 (3) ◽  
pp. 539-545
Author(s):  
Vo Thi Thuong Lan ◽  
Le Thi Thanh
Keyword(s):  
Molecular Biology ◽  
Restriction Enzyme ◽  
Dna Markers ◽  
Dna Marker ◽  
Dna Fragments ◽  
Dna Ladder ◽  
Time Saving ◽  
Pcr Product ◽  
Dna Fragment

DNA marker is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare recombinant plasmids pSY-60 which was partially digested by one restriction enzyme for generating DNA markers of 7 fragments from 60 to 420 bp. The procedure included a synthesis of 60 bp DNA fragment with EcoRI sites at both ends using PCR extension, self-ligation of the 60 bp fragments and subcloning the ligated product into plasmid, generating recombinant pSY-60. Once being cloned, 500 ng of 420 bp fragment purified from 100 µL PCR product was incompletely digested by EcoRI, sufficiently producing to 50 acrylamide gels. Our procedure for production of DNA markers could be simple, time saving and inexpensive in comparison with current ones widely used in most laboratories.

scholarly journals A putative resistant DNA marker for wool yellowing susceptibility in sheep

2000 ◽  
Vol 23 (2) ◽  
pp. 341-346
Author(s):  
M.V. Benavides ◽  
S. Damak ◽  
A.P. Maher
Keyword(s):  
Candidate Genes ◽  
Differential Display ◽  
Dna Sequences ◽  
Dna Markers ◽  
Dna Marker ◽  
Transmembrane Signalling ◽  
Breeding Values ◽  
Wide Range ◽  
Resistant Group ◽  
Australian Merino

An Australian Merino flock was screened for low (resistant) and high (susceptible) yellow predictive colour (YPC) breeding values in order to compare extreme individuals using the differential display of mRNA technique. One differentially expressed cDNA band was visualised only in the resistant group. This band showed no identity with the DNA sequences of public databases; however, they showed short homologies with three database sequences related to transmembrane signalling functions. The use of these candidate genes as DNA markers needs to be confirmed against sheep with a wide range of susceptibility to wool yellowing to verify the results.

scholarly journals RELATIONSHIP OF GROWTH HORMONE GENE WITH SOME OF PRODUCTIVE TRAITS OF COMMON CARP Cyprinus carpio.L

2018 ◽  
Vol 49 (6) ◽  
Author(s):  
AL-Azzawy & Al-Khshali
Keyword(s):  
Growth Rate ◽  
Common Carp ◽  
Growth Traits ◽  
Direct Sequencing ◽  
Growth Hormone Gene ◽  
Daily Growth ◽  
Total Weight Gain ◽  
Gh Gene ◽  
Productive Traits ◽  
Relationship Of

This study was carried out at Al-Radhwaniyah fish Reservoir (Baghdad) to investigate the polymorphism of GH gene and relationship with some of productive characteristics (total weight gain (T.W.G), daily growth rate (D.G.R), relative growth rate (R.G.R) and specific growth rate (S.G.R) in common carp. single nucleotide polymorphism (SNPs) in GH gene was analyzed by direct sequencing. Two SNP were identified in the third intron of GH1, the first SNP at site A1132T was negative correlated with growth traits, AA Genotype (wild)  was significant(p<0.05) correlated with growth traits. The second SNP was happened at site of G1217T, any genotype significantly does not correlated with growth traits. the study summarized that identification of SNPs associated with growth performance can be candidate as genetics markers in marker-assisted selection (MAS) programs for improving growth traits in common carp.

scholarly journals THE PROBLEM OF DETECTION OF HETEROZYGOUS GENOTYPES BY MUTATION OF THE FAD 2-1 GENE OF SUNFLOWER (HELIANTHUS ANNUUS L.) USING DNA MARKERS

2021 ◽  
Author(s):  
D.L. Savichenko ◽  
◽  
S.Z. Guchetl ◽  
Keyword(s):  
Dna Markers ◽  
Mutant Allele ◽  
Dna Marker ◽  
Oleic Acid Content ◽  
Wild Type ◽  
High Oleic Acid ◽  
Marker System ◽  
High Oleic ◽  
High Oleic Acid Content ◽  
Search For Information

The development of marker-associated breeding requires the development of DNA marker systems that meet the needs of the breeding process. We are looking for the opportunities to improve the efficiency of breeding for high oleic acid content in sunflower oil. One of the directions is the improvement of the existing marker system for detection the heterozygous status of the alleles of the FAD 2-1 gene. We carried out the search for information published in databases on this DNA locus. We studied the structure of the nucleotide sequences of the mutant allele and the wild-type allele. We proposed the way of improving the existing marker system.

scholarly journals Identification and Monitoring of Amomi Fructus and its Adulterants Based on DNA Barcoding Analysis and Designed DNA Markers

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4193 ◽  
Author(s):  
Eui Jeong Doh ◽  
Jung-Hoon Kim ◽  
Guemsan Lee
Keyword(s):  
Dna Barcoding ◽  
Dna Markers ◽  
Dna Marker ◽  
Intergenic Spacer ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Traditional Medicines ◽  
Amomum Villosum ◽  
Reliable Methods ◽  
A New Species

Amomi Fructus is one of the traditional medicines derived from the ripe fruits of the Zingiberaceae family of plants, which include Amomum villosum, A. villosum var. xanthioides, and A. longiligulare. Owing to their highly similar morphological traits, several kinds of adulterants of Amomi Fructus have been reported. Therefore, accurate and reliable methods of identification are necessary in order to ensure drug safety and quality. We performed DNA barcoding using five regions (ITS, matK, rbcL, rpoB, and trnL-F intergenic spacer) of 23 Amomi Fructus samples and 22 adulterants. We designed specific DNA markers for Amomi Fructus based on the single nucleotide polymorphisms (SNPs) in the ITS. Amomi Fructus was well separated from the adulterants and was classified with the species of origin based on the detected SNPs from the DNA barcoding results. The AVF1/ISR DNA marker for A. villosum produced a 270 bases amplified product, while the ALF1/ISF DNA marker produced a 350 bases product specific for A. longiligulare. Using these DNA markers, the monitoring of commercially distributed Amomi Fructus was performed, and the monitoring results were confirmed by ITS analysis. This method identified samples that were from incorrect origins, and a new species of adulterant was also identified. These results confirmed the accuracy and efficiency of the designed DNA markers; this method may be used as an efficient tool for the identification and verification of Amomi Fructus.

DNA polymorphism in the stem nematode Ditylenchus dipsaci: development of diagnostic markers for normal and giant races

Genome ◽  
2003 ◽  
Vol 46 (6) ◽  
pp. 1077-1083 ◽  
Author(s):  
Magali Esquibet ◽  
Eric Grenier ◽  
Olivier Plantard ◽  
Fouad Abbad Andaloussi ◽  
Georges Caubel
Keyword(s):  
Vicia Faba ◽  
Dna Polymorphism ◽  
Distinct Species ◽  
Genetic Distances ◽  
Diagnostic Markers ◽  
Intraspecific Polymorphism ◽  
Efficient Tool ◽  
Normal Populations ◽  
Ditylenchus Dipsaci ◽  
Two Populations

DNA polymorphism in the Ditylenchus dipsaci complex was investigated using amplified fragment length polymorphism (AFLP) to determine the relationships among populations growing mainly on Vicia faba and to develop diagnostic markers. Twenty-two populations of D. dipsaci originating from different geographical areas and one population of Ditylenchus myceliophagus were used. AFLP proved to be a powerful method to reveal intraspecific polymorphism even within the giant type. The analysis showed a clear distinction between the giant and normal populations, with genetic distances similar to those observed between normal populations and D. myceliophagus or giant populations and D. myceliophagus, strengthening the hypothesis that these two nematode types could be considered distinct species. Two specific AFLP markers differentiating the two types were converted into sequenced characterized amplified region (SCAR) markers. Used in a multiplex PCR, the SCAR primers proved to be a rapid and efficient tool to identify the giant and the normal types of D. dipsaci.Key words: Ditylenchus dipsaci, Vicia faba, giant type, AFLP, SCAR.

scholarly journals Cloning and Expression of hGAD65 Gene in E. Coli BL21

2015 ◽  
Vol 18 (1) ◽  
pp. 52
Author(s):  
Rista Nikmatu Rohmah ◽  
Soraya Widyasari ◽  
A. Aulanni’am ◽  
F. Fatchiyah
Keyword(s):  
Monoclonal Antibody ◽  
Protein Expression ◽  
Gold Standard ◽  
Restriction Enzymes ◽  
Cloning And Expression ◽  
Normal Person ◽  
Specific Primer ◽  
Salting Out ◽  
E Coli ◽  
Pcr Product

The aim of this study is to construct the hGAD65 gene and to identify the hGAD65 clone by using PCR & RFLP. The samples were derived from normal person & DM patient’s blood. Blood DNA was isolated by salting out method and then amplified by PCR with a pair of specific primer, GAD65-F-BamH1-807 & GAD65-R-Xho1-945. The PCR-product was cloned into vector pET-28a and the pET28a-hGAD65-clone was transformed into E.coli BL21 competent cells. The pET28a-hGAD65-clone was confirmed by PCR and RFLP by BamH1 & XhoI. The PCR product of pET28a-hGAD65-clone was one band of 159bp and has two bands 5.3 kb and 159 bp by RFLPwith both restriction enzymes. The GAD65 protein is expressed in 65kD of pET28a-hGAD65-clone. PET28a-hGAD65-clone was able to recognize by gold standard monoclonal antibody specifically. These results indicated that the hGAD65 gene inserted into pET28a properly and provided the GAD65 protein expression. Key words: hGAD65, PCR, pET-28a, RFLP

scholarly journals IDENTIFIKASI POLIMORFISME GEN GH (GROWTH HORMONE) SAPI BALI DENGAN METODE PCR-RFLP

2006 ◽  
Vol 12 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Sri Rahayu ◽  
Sutiman Bambang Sumitro ◽  
T Susilawati ◽  
Soemarno Soemarno
Keyword(s):  
Growth Hormone ◽  
Restriction Enzyme ◽  
Growth Hormone Gene ◽  
Salting Out ◽  
Pcr Product ◽  
Pcr Rflp ◽  
Reverse Primer ◽  
Total Dna ◽  
Polymerase Chain ◽  
Forward Primer

This study was conducted to identify polymorphism of growth hormone gene of Bali cattle. A PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) procedure was developed for determining polymorphism of growth hormone gene. The DNA was isolated from blood samples by salting out method. Total DNA were amplified with forward primer, 5’-TAGGGGAGGGTGGAAAATGGA-3’ and reverse primer, 5’-GACACCTACTCAGACAATGCG-3’. The PCR product was digested by HaeIII restriction enzyme. Result of the amplification was a specific single band with fragment 450 bp. Restriction with HaeIII restriction enzyme resulted four kinds of haplotype. Haplotype I was not cut by HaeIII restriction enzyme. Haplotype II were cut into two, 225 bp and 150 bp,. Haplotype III were cut into three size, 400 bp, 225 bp and 150 bp. Haplotype IV were cut into five fragments 450 bp, 400 bp, 275 bp, 225 bp and 150 bp.

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