scholarly journals Biochemical and microbiological quality of fermented silage prepared from dressing waste of Indian major carps using different concentrations of jaggery

2016 ◽  
Vol 63 (3) ◽  
Author(s):  
S. Tanuja ◽  
Anil Kumar ◽  
A. Sarkar ◽  
Sujit K. Nayak

Present study investigated the shelf life of fermented silage prepared from dressing waste (gut and gills) of Indian major carps and the effect of different concentrations of jaggery as carbohydrate source, on the biochemical and microbiological changes in the silage. Fermented silage was prepared using jaggery at 10% w/w (F1) and 20% w/w (F2) concentrations. F2 had a lower crude fat and ash content. The lowest pH attained was 4.11 and 4.02 in F1 and F2 respectively by the end of 15 days. Significantly higher (p<0.05) concentration of total volatile base nitrogen (TVB-N) was observed in F2 from the 30th day of storage. The rate of auto-oxidation was also significantly higher in F2 throughout the storage period of 60 days. Total plate count (TPC) of bacteria in F1 and F2 reduced significantly till the 15th day of storage. At the end of 60 days of storage, pH reached 5.55 and 5.67 in F1 and F2 respectively, thereafter the silage was considered spoiled. The study indicated that fermented fish silage prepared from viscera and gills of Indian major caps remained shelf worthy for a period of 60 days and the use of jaggery at concentrations higher than 20% (w/w) would not be viable as it led to faster spoilage rate and increased cost of production

2008 ◽  
pp. 67-75
Author(s):  
Ferenc Peles ◽  
Zsuzsa Máthéné Szigeti ◽  
Béla Béri ◽  
András Szabó

The importance of the quality of raw milk increased after Hungary had joined to the EU. On delivery of raw milk, the microbiological quality, especially total plate count of the milk is very important. Twenty-two farms (7 large, 4 medium-sized, and 11 small farms) were included in the study. We considered the different farm size, keeping- and milking circumstances during the selection of farms. The examined large farms use loose housing system (cubicle, deep litter) and milking parlour. Most of them use preand post-milking disinfection. In the medium-sized farms, loose,deep litter and tie-stall housing system, as well as milking parlour, pipeline milking and bucket milking occurred. All of them use preand post-milking disinfection. Small farms use tie-stall housing system, bucket milking and udder preparation by water. Unfortunately, they do not use pre- or post-milking disinfection. In the large and medium-sized farms mainly Holstein Friesian, in the small farms Hungarian Simmental breeds can be found.The aim of our research was to examine the microbiological status of the raw milk produced in dairy farms (total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, psychrotroph bacteria count, furthermore yeast and mold count); sources of the contamination; connection between the microbiological quality of produced milk and housing-, milking technologies of farms; furthermore the hygienic circumstances of milking and milk handling of the farms, by the examination of coliform bacteria and Escherichia coli contamination.During the examination of the connection between the different farm sizes, various housing- and milking forms and the microbiological characteristics we observed similar tendencies in the case of total plate count, coliform count, yeast and molds count, furthermore psychrotroph bacteria count. The value of  these parameters was significantly higher in small farms, and infarms which use tie-stall housing forms, bucket milking, udder preparation with water, and which do not use pre- and post-milking disinfection.The results showed that besides cooling, the milking procedure and the type of udder preparation had the largest effect on the total plate count. Statistical analysis shows that in medium and small farms the combination of pipeline milking – tie stall housing system – disinfectant preparation of the udder; in large farms the combination of milking parlour – loose cubicle housing system – dry preparation of the udder are the most appropriate in the aspect of the total plate count. We experienced that in farms where the hygienic instructions are not followed – and thereforeequipment used during the milking and handling of milk is very contaminated – or rather the separation of mastitic cows’ milk is not appropriate, different microorganisms may contaminate the produced milk. 


2021 ◽  
Vol 36 (1) ◽  
pp. 155
Author(s):  
Rohula Utami ◽  
Kawiji Kawiji ◽  
Windi Atmaka ◽  
Lulus Nurmaya ◽  
Lia Umi Khasanah ◽  
...  

Strawberry is a popular and nutritious fruit. However, its improper post-harvest handling leads to quality degradation. The aim of this study was to investigate the effects of active paper packaging placements methods on strawberry (<em>Fragaria x ananassa</em>) quality during refrigerated storage. Strawberry quality was determined based on the physical (weight loss, hardness, color), chemical (pH, total titratable acid, total soluble solids and vitamin C content) and microbiological (total plate count) properties. The results of this study indicated that different placement methods of active paper packaging had no significant effect on the physical and chemical qualities; but it did affect the microbiological quality of the strawberries. At the end of the experiment (12 days), the microbial content of C (the bottom and walls), D (covering all surfaces) and E (cut into pieces) samples were significantly lower than the A (without active paper packaging) and B (the bottom) samples. The Total Plate Count (TPC) values of the control and B samples were higher than the maximum acceptable limit of 6 log10 colony-forming units (CFU) g<sup>-1</sup>, while the other samples remained below the limit. These results indicated that a larger surface area of active paper that had contact with the strawberries provided better protection against microbes. Due to their microbial properties, C, D and E treatments maintained the strawberry quality for up to 12 days, compared with 9 days for control and B treatments. Active paper packaging containing oleoresin from solid waste of pressed <em>Curcuma xanthorrhiza</em> Roxb. can be applied to extend the shelf life of strawberries during refrigerated storage.


Author(s):  
Vikas Kumar ◽  
D. Sukumar ◽  
M. Muruganantham

Squids perish rapidly like other fishes and require some treatment to maintain the quality for export. The microbial quality of frozen squid (Sepioteuthis lessoniana, Lesson 1830) treated with the commercial food grade chemicals imported from Spain was therefore studied. Dressed squids were treated with the chemicals Hidratech_4A (0.4%) and Whitech_3 (0.25%) dissolved in chilled freshwater (STCF) and chilled saltwater (STCS). Chilled squids not treated served as control (SNTC). Squids were quick frozen at -40°C in contact plate freezer and stored at-20±1°C. Samples were tested raw, after pre-processing, treatments and freezing and during storage at monthly intervals for 7 months. The microbial quality evaluation included Total Plate Count (TPC), Escherichia coli, Staphylococcus aureus, Vibrio cholerae and Salmonella. Study revealed a better quality of treated samples than control. Microbiological quality of STCS was better than STCF and SNTC. E. coli counts decreased after treatment. Salmonella and V. cholerae were absent.


2013 ◽  
Vol 57 (4) ◽  
pp. 535-543 ◽  
Author(s):  
Elżbieta Kukier ◽  
Magdalena Goldsztejn ◽  
Tomasz Grenda ◽  
Krzysztof Kwiatek ◽  
Łukasz Bocian

Abstract The study was conducted at all regional veterinary diagnostic laboratories. Feed materials were examined for Salmonella prevalence and contamination by Enterobacteriaceae, aerobic mesophilic bacteria, total plate count, fungi, Clostridium sp., and Bacillus cereus. Assays were done following international and Polish standards used in food and feed microbiology. Salmonella sp. were most often detected in oil seeds. In most of the examined feed ingredients, the number of Enterobacteriaceae did not exceed 10 cfu/g. The contamination by aerobic bacteria ranged most often from 101to 107 cfu/g, and the highest mycological contamination was noted in cereal grains (108 cfu/g). The results showed that microbial contamination of feed materials in regard to Enterobacteriaceae, fungi, and total plate counts declined over the past years.


Author(s):  
Diah Ikasari ◽  
Theresia Dwi Suryaningrum

Quality asessment of pangasius (Pangasius hypopthalmus) fillets stored in ice has been conducted. Fish were fasted for one day and slaughtered using bleeding techniques, drained for 10 minutes and filleted in various types of fillet: skin on, skinless, trimmed and untrimmed condition. Fillets were then washed and packed in vacuum plastics, stored in ice (0-4 ºC) for 18 days and observed for its sensory, chemical and microbiological parameters every 3 days. The sensory evaluation was conducted both for fresh or cooked fillets using scoring test on attribute of appearance, odor and texture as well as hedonic test. The chemical parameters observed were proximate; pH and Total Volatile Base (TVB); while microbiological parameter was Total Plate Count (TPC). Results showed that type of fillet did not significantly affect the quality of pangasius fillets. The quality of all treated fillet decreased in line with time of storage, all products were rejected after being stored for 18 days. At the time of rejection, the quality parameters: moisture content ranging from 80.1 to 81.3%, TVB from 11.1 to 11.5 mg N/100g and TPC from 1.41 to 4.6x105 CFU/ml. It is suggested to preserve pangasius fillets in ice less than 18 days.


Author(s):  
Hue Thi Luu ◽  
Chris M. Michiels

The aim of this study is to analyze and document the microbiological safety and quality of ready-to-eat foods in hospital and university canteens in Hanoi, Vietnam. A total of 420 ready-to-eat food products from 21 canteens were sampled in July 2018 and May 2019. The ratio of samples exceeding the unsatisfactory level for Total Plate Count (TPC) was 31%. Escherichia coli, Listeria and Staphylococcus aureus were detected in 35 (8.3%), 99 (24%), 46 (11%) samples, with 3%, 10% and 0% exceeding the unsatisfactory level, respectively. The Total Plate Count (TPC), Listeria, Bacillus cereus, E. coli, S. aureus ranged from below detection limit to 5x10 9 , 4.6x10 5 , 6.2x10 3 , 3.4x10 3 , 7.6x10 3 CFU/g, respectively. Listeria monocytogenes was isolated from 3/420 samples (0.7%). In addition, there were 21 out of 410 samples (5%) contaminated with Salmonella. Overall, our data indicate frequent problems with the microbiological quality and safety of these canteen foods in Hanoi, and provide a baseline measurement that will allow environmental health officers and food microbiologists to develop targeted intervention strategies to reduce the economical and public health risk associated with these foods.


2014 ◽  
Vol 11 (1) ◽  
pp. 35-43 ◽  
Author(s):  
AD Kolekar ◽  
AU Pagarkar

Fish ball in curry (FBC) was prepared following standard recipe. Prepared FBC was packed in standup pouches and stored at 0 to -2°C. The organoleptic scores for overall acceptability of the FBC were slightly decreased within the storage period (12 days). The pH decreased from 6.38 to 6.07, while peroxide value increased from 2.27 to 9.47. The total volatile base-nitrogen (TVB-N) increased from 5.04 to 17.64. The total plate count increased from an initial value of 1.8 x 102 to 2.4 x 104 cfu g-1 during chilled storage. The Staphylcoccus aureus, Escherichia coli and Salmonella sp. were not detected throughout the study period. It was observed that the products stored at 0 to -2°C were acceptable organoleptically, and stable biochemically and microbiologically up to 9 days. DOI: http://dx.doi.org/10.3329/sja.v11i1.18373 SAARC J. Agri., 11(1): 35-43 (2013)


2020 ◽  
Vol 18 (1) ◽  
pp. 31
Author(s):  
SARI INTAN KAILAKU ◽  
TATANG HIDAYAT ◽  
DONDY A. SETIABUDY

<p>ABSTRAK<br />Produk santan awet semakin banyak digunakan masyarakat untuk<br />alasan kepraktisan. Salah satu cara untuk menstabilkan emulsi santan yaitu<br />dengan penambahan emulsifier/stabiliser dan proses homogenisasi.<br />Penelitian ini bertujuan untuk mendapatkan kondisi proses homogenisasi<br />yang dapat menghasilkan emulsi santan yang stabil dengan masa simpan<br />yang optimal. Perlakuan yang diterapkan yaitu tiga kecepatan putaran<br />(6.000, 11.000, dan 16.000 rpm) dan empat durasi homogenisasi (10, 20,<br />30, dan 40 menit). Percobaan menggunakan rancangan acak lengkap<br />(RAL) faktorial dengan tiga kali ulangan. Parameter yang diamati yaitu<br />karakteristik fisik emulsi santan yang meliputi stabilitas emulsi, viskositas,<br />dan mikroskopik santan. Kemudian diamati pula mutu santan selama<br />penyimpanan dengan menguji parameter pH, warna, kadar asam lemak<br />bebas, dan total mikroba. Emulsifier yang digunakan adalah Tween 20<br />(konsentrasi 0; 0,5; dan 1%) dan karboksi-metilselulosa-CMC (konsentrasi<br />0 dan 0,6%). Hasil penelitian menunjukkan bahwa penggunaan CMC 0,6%<br />dapat menghasilkan stabilitas emulsi yang baik (100%) sampai dengan<br />akhir pengamatan (14 hari). Kondisi proses homogenisasi yang optimal<br />diperoleh pada kecepatan putaran 6.000 rpm selama 30 menit. Mutu santan<br />yang dihasilkan dari kondisi proses homogenisasi yang optimal cukup baik<br />dan layak dikonsumsi sampai dengan penyimpanan minggu ke-6.<br />Kata kunci: Santan, homogenisasi, emulsifier/stabiliser, karboksi-metil-<br />selulosa (CMC), mutu</p><p>ABSTRACT<br />Preserved coconut milk is increasingly used publicly because of its<br />practical reasons. One of the ways to stabilize the coconut milk emulsion<br />is by addition of emulsifier/stabilizer and homogenizing process. This<br />study aimed at obtaining conditions of the homogenization process to<br />produce stable emulsions of coconut milk with optimum storage period.<br />The treatments applied were three rotation speeds (6,000, 11,000, and<br />16,000 rpm) and four homogenization durations (10, 20, 30, and 40<br />minutes). The experiment was arranged using factorial completely<br />randomized design (CRD) factorial with three replicates .  Parameters<br />observed were physical characteristics including coconut milk emulsion<br />stability, viscosity, and microscopic test of milk. Furthermore, it was then<br />observed quality of the coconut milk during storage by testing the<br />parameters of pH, color, free fatty acid (FFA) levels, and total microbes by<br />total plate count (TPC). Emulsifiers used in the experiment were Tween 20<br />(concentrations of 0, 0.5, and 1%) and carboxy-methylcellulose - CMC<br />(concentrations of 0 and 0.6%). The results showed that the use of 0.6%<br />CMC was able to produce a good emulsion stability (100%) until the end<br />of the observation (14 days). Optimal condition of homogenization<br />process was obtained at the rotation speed of 6,000 rpm for 30 minutes.<br />The quality of coconut milk resulted from optimal condition of<br />homogenization process was quite good and valid for consumption up to 6<br />weeks of storage.<br />Key words : Coconut  milk,  homogenization,  emulsifier/stabilizer,<br />carboxy-methylcellulose (CMC), quality.</p>


2017 ◽  
Vol 5 (1) ◽  
pp. 13
Author(s):  
Septian Gedoan Bentalen ◽  
Hens Onibala ◽  
Netty Salindeho

The purpose of this study was to determine the quality of smoked skipjack (Katsuwonus pelamis L) smoke pre-treated in a solution of mangosteen peel with a concentration of 3, 4 and 5%, with various soaking time, then stored and observed at 0, 3, 6, and 9 days. The results showed that the highest value of total plate count (TPC) was found in the fish sample storage for 9 days, soaking with a concentration of 3% for 30 minutes. Meanwhile, the lowest TPC value was discovered for the fish samples that did not undergo storage, and immersed in a solution of 5% for 30 minutes. Furthermore, the highest TVB value (Total Volatile bases) was detected in fish samples soaked with 3% solution of the mangosteen for 15 minutes (59.22mg N/100g) while the lowest value obtained on the fish sample marinated with 5% concentration for 15 minutes and did not experience storage (5.04mg N/100g).


2021 ◽  
Vol 32 (2) ◽  
pp. 213-220
Author(s):  
KRISANTO L. BACNUTAN ◽  
KIRBY ULYSSES M. MOMO ◽  
RYAN JAMES A. PAGTABUNAN ◽  
JERSON SORIO

Bivalves are filter feeders that ingest particles from the surrounding water, including pathogens,which could cause illness in consumers. In Samar, Philippines no data on the microbial quality of undulatedsurf clam (Paphia undulata) have previously been reported. Thus, this study was conducted to determine themicrobial quality of the species in selected areas in Samar, Philippines (Pinabacdao, Zumarraga andVillareal). The total plate count (TPC) and total Vibrio count were beyond the standard limits set by the Foodand Drug Administration (FDA), although Salmonella was not detected in any samples. The TPC ofundulated surf clam was 5.9-6.3 log CFU·g-1 in Pinabacdao, 4.9-6.0 log CFU·g-1 in Zumarraga, and 6.3-7.2log CFU·g-1 in Villareal. The total Vibrio count was 5.1-5.7 log CFU·g-1 in Pinabacdao, 3.9-4.6 log CFU·g-1in Zumarraga, and 6.1 log CFU·g-1 in Villareal. Bivalves in these areas should undergo purification process,such as relaying or depuration


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