scholarly journals Microbiological quality of undulated surf clam (Paphia undulata) in selected areas in Samar, Philippines

2021 ◽  
Vol 32 (2) ◽  
pp. 213-220
Author(s):  
KRISANTO L. BACNUTAN ◽  
KIRBY ULYSSES M. MOMO ◽  
RYAN JAMES A. PAGTABUNAN ◽  
JERSON SORIO

Bivalves are filter feeders that ingest particles from the surrounding water, including pathogens,which could cause illness in consumers. In Samar, Philippines no data on the microbial quality of undulatedsurf clam (Paphia undulata) have previously been reported. Thus, this study was conducted to determine themicrobial quality of the species in selected areas in Samar, Philippines (Pinabacdao, Zumarraga andVillareal). The total plate count (TPC) and total Vibrio count were beyond the standard limits set by the Foodand Drug Administration (FDA), although Salmonella was not detected in any samples. The TPC ofundulated surf clam was 5.9-6.3 log CFU·g-1 in Pinabacdao, 4.9-6.0 log CFU·g-1 in Zumarraga, and 6.3-7.2log CFU·g-1 in Villareal. The total Vibrio count was 5.1-5.7 log CFU·g-1 in Pinabacdao, 3.9-4.6 log CFU·g-1in Zumarraga, and 6.1 log CFU·g-1 in Villareal. Bivalves in these areas should undergo purification process,such as relaying or depuration

Author(s):  
Vikas Kumar ◽  
D. Sukumar ◽  
M. Muruganantham

Squids perish rapidly like other fishes and require some treatment to maintain the quality for export. The microbial quality of frozen squid (Sepioteuthis lessoniana, Lesson 1830) treated with the commercial food grade chemicals imported from Spain was therefore studied. Dressed squids were treated with the chemicals Hidratech_4A (0.4%) and Whitech_3 (0.25%) dissolved in chilled freshwater (STCF) and chilled saltwater (STCS). Chilled squids not treated served as control (SNTC). Squids were quick frozen at -40°C in contact plate freezer and stored at-20±1°C. Samples were tested raw, after pre-processing, treatments and freezing and during storage at monthly intervals for 7 months. The microbial quality evaluation included Total Plate Count (TPC), Escherichia coli, Staphylococcus aureus, Vibrio cholerae and Salmonella. Study revealed a better quality of treated samples than control. Microbiological quality of STCS was better than STCF and SNTC. E. coli counts decreased after treatment. Salmonella and V. cholerae were absent.


Author(s):  
Anil Gill ◽  
Akash John ◽  
Neelam Iqbal ◽  
Tallat Anwar Faridi ◽  
Sofia Noor

Bakery items are a vital element of a well-balanced diet, and supermarket shelves now stock a wide range of them. Bakery items are the most popular ready-to-eat foods (REF).Objective:To assess the microbial quality of bakery foods (Bread, Cake and patties) and check the total plate count (TPC), bacterial and yeast count Methods:The analysis of the microbial quality of bakery products was determined for three different bakery products (bread, cake and patties) at ten different locations collected from a renowned bakery in Lahore, Pakistan. The samples were assessed under complete hygienic conditions by standardized plate count (TPC) and MPN method. Micro-organisms (Coliform, Fecal Coliform and Yeast) were lately confirmed by their morphological characteristics and biochemical tests Results: Among the three commodities, bread was found to be highly contaminated by micro-organisms and the total plate count recorded was 4.7 x 105cfu g-1collected from sector I9 which is not consumable. Whereas Patties showed the number of TPC as 1.6 x 105 cfu g-1also collected from sector I9. Cake was revealed as least infected bakery product i.e., 5.7 x 104 cfu g-1. The above results showed that sector I9 is highly contaminated and a threat for health. Bacterial plate count (Coliform and Fecal Coliform count) was highly measured in bread as 460 MPN g-1 taken from sector I5 where as it remains under the permissible limit determined by WHO, 1994 in remaining sectors Conclusions:In conclusion the bakery food is contaminated by the use of un-hygienic water, miss handled during the process after baking i.e., slicing, serving and packaging. To avoid a possible epidemic of food borne illness, competent authorities should conduct a stricter and more stringent inspection of  REFs sold to clients in bakeries.


2008 ◽  
pp. 67-75
Author(s):  
Ferenc Peles ◽  
Zsuzsa Máthéné Szigeti ◽  
Béla Béri ◽  
András Szabó

The importance of the quality of raw milk increased after Hungary had joined to the EU. On delivery of raw milk, the microbiological quality, especially total plate count of the milk is very important. Twenty-two farms (7 large, 4 medium-sized, and 11 small farms) were included in the study. We considered the different farm size, keeping- and milking circumstances during the selection of farms. The examined large farms use loose housing system (cubicle, deep litter) and milking parlour. Most of them use preand post-milking disinfection. In the medium-sized farms, loose,deep litter and tie-stall housing system, as well as milking parlour, pipeline milking and bucket milking occurred. All of them use preand post-milking disinfection. Small farms use tie-stall housing system, bucket milking and udder preparation by water. Unfortunately, they do not use pre- or post-milking disinfection. In the large and medium-sized farms mainly Holstein Friesian, in the small farms Hungarian Simmental breeds can be found.The aim of our research was to examine the microbiological status of the raw milk produced in dairy farms (total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, psychrotroph bacteria count, furthermore yeast and mold count); sources of the contamination; connection between the microbiological quality of produced milk and housing-, milking technologies of farms; furthermore the hygienic circumstances of milking and milk handling of the farms, by the examination of coliform bacteria and Escherichia coli contamination.During the examination of the connection between the different farm sizes, various housing- and milking forms and the microbiological characteristics we observed similar tendencies in the case of total plate count, coliform count, yeast and molds count, furthermore psychrotroph bacteria count. The value of  these parameters was significantly higher in small farms, and infarms which use tie-stall housing forms, bucket milking, udder preparation with water, and which do not use pre- and post-milking disinfection.The results showed that besides cooling, the milking procedure and the type of udder preparation had the largest effect on the total plate count. Statistical analysis shows that in medium and small farms the combination of pipeline milking – tie stall housing system – disinfectant preparation of the udder; in large farms the combination of milking parlour – loose cubicle housing system – dry preparation of the udder are the most appropriate in the aspect of the total plate count. We experienced that in farms where the hygienic instructions are not followed – and thereforeequipment used during the milking and handling of milk is very contaminated – or rather the separation of mastitic cows’ milk is not appropriate, different microorganisms may contaminate the produced milk. 


2021 ◽  
Vol 36 (1) ◽  
pp. 155
Author(s):  
Rohula Utami ◽  
Kawiji Kawiji ◽  
Windi Atmaka ◽  
Lulus Nurmaya ◽  
Lia Umi Khasanah ◽  
...  

Strawberry is a popular and nutritious fruit. However, its improper post-harvest handling leads to quality degradation. The aim of this study was to investigate the effects of active paper packaging placements methods on strawberry (<em>Fragaria x ananassa</em>) quality during refrigerated storage. Strawberry quality was determined based on the physical (weight loss, hardness, color), chemical (pH, total titratable acid, total soluble solids and vitamin C content) and microbiological (total plate count) properties. The results of this study indicated that different placement methods of active paper packaging had no significant effect on the physical and chemical qualities; but it did affect the microbiological quality of the strawberries. At the end of the experiment (12 days), the microbial content of C (the bottom and walls), D (covering all surfaces) and E (cut into pieces) samples were significantly lower than the A (without active paper packaging) and B (the bottom) samples. The Total Plate Count (TPC) values of the control and B samples were higher than the maximum acceptable limit of 6 log10 colony-forming units (CFU) g<sup>-1</sup>, while the other samples remained below the limit. These results indicated that a larger surface area of active paper that had contact with the strawberries provided better protection against microbes. Due to their microbial properties, C, D and E treatments maintained the strawberry quality for up to 12 days, compared with 9 days for control and B treatments. Active paper packaging containing oleoresin from solid waste of pressed <em>Curcuma xanthorrhiza</em> Roxb. can be applied to extend the shelf life of strawberries during refrigerated storage.


2020 ◽  
Vol 39 (01) ◽  
Author(s):  
Pankaj Kumar Gupta ◽  
Dinesh Chandra Rai ◽  
Vinod Kumar Paswan ◽  
Rajendra Panta ◽  
Ashok Kumar Yadav

The present study was conducted to evaluate the physico-chemical characteristics including Adulteration and Microbiological quality of cow raw milk collected from four different places of ASSI region in Varanasi. Samples were analysed to know the chemical composition, the results showed that the statistically average percentage of Moisture (87.46), Fat (3.87), Protein (3.15), Lactose (4.42), Ash (.712), pH (6.43) and acidity (0.147). The keeping quality of milk was evaluated by Methylene Blue Reduction Test (MBRT). This phenomenon testified that milk sample 1 is fair quality and remained sample were found good and excellent. The microbiological conclusion confirmed the presence (less or more) of microbial load in all the raw milk samples. The highest level of microbial quality in standard plate count (SPC) was 19.1×106 cfu/ml. in sample 1 and in logarithm value is 7.28 cfu/ml at the same time, the highest coliform bacteria 2.3×102 in logarithm value is 2.36 was found in the sample 2. The adulterations in raw milk were checked by the standard procedure. In cow’s raw milk the different mixed adulterant were found in two samples contaminated with detergent and pulverized soap. Besides different hazardous chemical adulterant, raw milk from sample 1 was detected with presence of hydrogen peroxide and sample 2 was contaminated formalin whereas urea was present in sample 2 and 4. Milk adulteration is a global concern and social problem. Increased demand, growth in competition in dairy industry and financial gain makes some producers to adulterate the milk thereby decreasing milk quality.


2013 ◽  
Vol 57 (4) ◽  
pp. 535-543 ◽  
Author(s):  
Elżbieta Kukier ◽  
Magdalena Goldsztejn ◽  
Tomasz Grenda ◽  
Krzysztof Kwiatek ◽  
Łukasz Bocian

Abstract The study was conducted at all regional veterinary diagnostic laboratories. Feed materials were examined for Salmonella prevalence and contamination by Enterobacteriaceae, aerobic mesophilic bacteria, total plate count, fungi, Clostridium sp., and Bacillus cereus. Assays were done following international and Polish standards used in food and feed microbiology. Salmonella sp. were most often detected in oil seeds. In most of the examined feed ingredients, the number of Enterobacteriaceae did not exceed 10 cfu/g. The contamination by aerobic bacteria ranged most often from 101to 107 cfu/g, and the highest mycological contamination was noted in cereal grains (108 cfu/g). The results showed that microbial contamination of feed materials in regard to Enterobacteriaceae, fungi, and total plate counts declined over the past years.


2012 ◽  
Vol 34 (3) ◽  
pp. 178
Author(s):  
Dellen Naomi Matulessy ◽  
Edi Suryanto ◽  
Rusman (Rusman)

<p>The experiment was conducted to evaluate the quality of frozen broiler carcass sold in traditional markets of North Halmahera. Samples were taken from three traditional markets that selling frozen broilers and five sellers for each market were chosen. Three carcasses were taken from each trader replications. Macroscopic observation was done by seven panelists to asses 14 carcasses from each seller. Parameters observed were macroscopic characteristis of carcasses i.e. conformation, fleshing, fat cover, wholeness, discoloration, defeathering, color, and aroma of carcass. Physical characteristis determined were pH, cooking loss, water-holding capacity, and tenderness. Chemical composition included water, fat, and protein contents, and microbial quality consisted of total microbial, Coliform and Escherichia coli bacteria. Macroscopic characteristic data were assessed using descriptive analysis based on National Standard, whereas physical quality, chemical and microbial quality data were analyzed using analysis of variance<br />completely randomized design. The differences between means, were tested using Duncan’s new Multiple Range Test. The results showed that the average macroscopic properties of conformation, fleshing, fat covering, wholeness,<br />discoloration, defeathering and the colour of frozen broiler carcasses with score I, were 40.41, 38.16, 50.41, 49.80, 49.59, and 50.82% respectively. Quality factor of the aroma/odor of a carcass with score II was 63.47%. The data of<br />physical quality of broiler meat were as follow: pH 6.16, cooking loss 21.72%, water-holding capacity 23.01%, and tenderness 2.81 kg/cm2. The chemical composition of broiler meat were moisture 71.93%, lipid 2.50% and protein<br />20.11%. Data of microbial quality of broiler carcass were total plate count 7.1x105 cfu/g, and E.coli 0.8x101 cfu/g, there was not any significant differences between the seller, however there was significant differences among the seller<br />on the number of coliform (P&lt;0.05). The conclusions of the experiment were that broiler carcasses sold in the traditional market in North Halmahera in respect of their physical characteristics and their chemical composition have not altered, whereas the microbial quality such as total plate count and E.coli number was still below threshold. Furthermore, coliform population was also below the maximum number allowed.</p><p><br />(Key words: Traditional market, Broilers frozen, Physical characteristics, Chemical composition, Microbial quality)<br /><br /></p>


Author(s):  
Hue Thi Luu ◽  
Chris M. Michiels

The aim of this study is to analyze and document the microbiological safety and quality of ready-to-eat foods in hospital and university canteens in Hanoi, Vietnam. A total of 420 ready-to-eat food products from 21 canteens were sampled in July 2018 and May 2019. The ratio of samples exceeding the unsatisfactory level for Total Plate Count (TPC) was 31%. Escherichia coli, Listeria and Staphylococcus aureus were detected in 35 (8.3%), 99 (24%), 46 (11%) samples, with 3%, 10% and 0% exceeding the unsatisfactory level, respectively. The Total Plate Count (TPC), Listeria, Bacillus cereus, E. coli, S. aureus ranged from below detection limit to 5x10 9 , 4.6x10 5 , 6.2x10 3 , 3.4x10 3 , 7.6x10 3 CFU/g, respectively. Listeria monocytogenes was isolated from 3/420 samples (0.7%). In addition, there were 21 out of 410 samples (5%) contaminated with Salmonella. Overall, our data indicate frequent problems with the microbiological quality and safety of these canteen foods in Hanoi, and provide a baseline measurement that will allow environmental health officers and food microbiologists to develop targeted intervention strategies to reduce the economical and public health risk associated with these foods.


Author(s):  
Vijayalakshmi S ◽  
Arun A ◽  
Kanchana Arun

Objective: Herbs have gained a widespread acceptance as a dietary constituent in Tamil Nadu, generally forming a substantial portion of the dietwhich serves as a therapeutic function. This study was aimed to formulate and standardize the four types of Rasam using four different herbs and toanalyze its quality changes in fresh Rasam and refrigerated Rasam (4±1°C). Appearance and odor scores of Rasam were satisfactory throughout thestorage. Sensory attributes were rated better for fresh Rasam than refrigerated Rasam, but all the attributes were decreased with increasing storageperiod.Methods: Microbiological quality of four types of different herbal Rasam was examined with freshly prepared Rasam and with refrigerated Rasam forthe presence of Escherichia coli, Staphylococcus aureus, Salmonella and Yeast, and Mold.Result: Comparison with the microbiological results shows in refrigerated Rasam-PAR, SAR, and AIR were at an acceptable level of microbiologicalquality whereas, Rasam-STR were at an unacceptable level of microbial quality due high level of total plate count and yeast and mold growth.Salmonella was examined, but it was not detected in all the four Rasam. Similarly, the microbial quality of all four freshly prepared Rasam showedhighly acceptable. Conductivity was measured for the presence of ions in solution.Conclusion: This study proposes that to minimize bacterial level in foods and always to consume freshly prepared Rasam than refrigerated. It alsoconcludes that good hygiene practices are necessary though the food serves a therapeutic function.


2018 ◽  
Vol 55 (2) ◽  
pp. 177
Author(s):  
N. Manimehalai ◽  
G. Rajesh ◽  
S. Balasubramanian ◽  
J. Durga Devi

The aim of this work is to examine the performance of gelatin based edible coating in preserving the seer fish slices. A composite edible coating solution was prepared with three different concentrations of gelatin, chitosan and vinegar. Coating experiments were performed in a laboratory scale edible coating tank which was fabricated for this purpose. The seer fish slices (13.65±2.50 mm thick and 43.89± 6.82 g weight) were subjected to coating for three different soaking times (min). While coating performance of the solution was assessed through coating uptake (%), texture quality of the seer fish slices was assessed through weight loss (%) and microbial quality was assessed through Total Plate Count (CFU/g). Experiment was designed using Box - Behnken Design (BBD). The application of edible coating treatment to seer fish slices resulted in improvement of overall keeping quality of the seer fish slices (9 days) against the control (3 days) when stored under low temperature (7±1oC).


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