scholarly journals PENGARUH IRADIASI SINAR GAMMA (60Co) TERHADAP PERTUMBUHAN DAN PRODUKSI JAHE PUTIH KECIL (Zingiber officinale var.a ma rum)

2020 ◽  
Vol 21 (2) ◽  
pp. 47
Author(s):  
NURLIANI BERMAWIE ◽  
NUR LAELA W. MEILAWATI ◽  
S. PURWIYANTI ◽  
MELATI MELATI

<p>ABSTRAK</p><p>Keragaman  genetik  plasma  nutfah  jahe  rendah  sehingga  perlu <br /> dilakukan  peningkatan  keragaman,  antara  lain  dengan  iradiasi sinar <br /> gamma. Penelitian ini bertujuan untuk mengetahui pengaruh dosis iradiasi <br /> terhadap pertumbuhan dan produksi jahe putih kecil.  Penelitian dilakukan <br /> di Laboratorium Balai Penelitian Tanaman Rempah dan Obat serta Kebun <br /> Percobaan Cicurug, Sukabumi pada bulan Juni 2010 sampai dengan Mei <br /> 2011. Iradiasi dilakukan di P3TIR, BATAN, Jakarta. Percobaan disusun <br /> menggunakan rancangan split plot dengan petak utama dua aksesi jahe <br /> putih kecil ZIOF 0048 dan HALINA 1 dan anak petak adalah 11 dosis <br /> iradiasi (0-50 Gy) dengan interval 5 Gy. Perlakuan diulang sebanyak tiga <br /> kali. Pengamatan karakter morfologi dilakukan secara kuantitatif dan <br /> kualitatif pada umur 2, 3, dan 4 BST. Hasil penelitian menunjukkan aksesi <br /> berpengaruh terhadap radiosensitivitas. Aksesi HALINA 1 lebih sensitif <br /> dibandingkan   dengan   ZIOF 0048   ditunjukkan   dengan   nilai   LD50 <br /> HALINA 1 (11,07 Gy) lebih rendah dari LD50 ZIOF 0048 (13,43 Gy). <br /> Interaksi aksesi pada dosis 5 dan 10 Gy memiliki nilai terbaik pada  tinggi <br /> tanaman, ukuran daun serta jumlah anakan. Dosis mempengaruhi tinggi <br /> tanaman, ukuran daun, diameter dan panjang batang, serta jumlah daun. <br /> Pertumbuhan tanaman semakin meningkat dengan bertambahnya umur <br /> tanaman. Namun, semakin tinggi dosis iradiasi, pertumbuhan tanaman <br /> semakin melambat.  Pertumbuhan tanaman terbaik diperoleh pada dosis 5 <br /> Gy, dan tidak berbeda dengan kontrol. Bobot dan ukuran rimpang terbaik <br /> (964 g/rumpun) diperoleh dari HALINA 1 pada dosis 10 Gy, dan berbeda <br /> dengan kontrol (454 g/rumpun), serta perlakuan lainnya.</p><p>Kata kunci:  Zingiber officinale var. amarum, iradiasi sinar gamma (<sup>60</sup>Co), keragaman genetik, mutasi, karakter morfologi.</p><p> </p><p>The Effect of Gamma Irradiation (<sup>60</sup>Co) on the Growth and Production of Small White Ginger (Zingiber officinale var. amarum)</p><p>ABSTRACT</p><p>Genetic variability of ginger germplasm was low, therefore it was <br /> necessary to broaden genetic variability using gamma irradiation. This <br /> research aimed to see the effect of irradiation on the growth of small white <br /> ginger. This study was conducted in the Plant Breeding Laboratory, <br /> Indonesian Spice and Medicinal Crops Research Institute and the Cicurug <br /> Experimental Garden Sukabumi from June 2010 to May 2011. Irradiation <br /> was undertaken in P3TIR, BATAN, Jakarta. The experiment was arranged <br /> in a split plot design with the main plot was two accessions HALINA 1 <br /> and ZIOF 00 48, and the sub plots was 11 irradiation doses (0-50 Gy) with 5 Gy interval.  The experiment was replicated three times.  Parameters <br /> observed were quantitative and qualitative characters at 2,3 and 4 months <br /> after   planting (MAPs).   Results   showed   that   accession   influenced <br /> radiosensitivity. Accession HALINA 1 was more radiosensitive than ZIOF <br /> 0048, indicated by LD50 of HALINA 1 was 11.0693 Gy, lower than ZIOF <br /> 0048 13.4254 Gy. Interaction between accessions at 5 and 10 Gy showed <br />the highest plant height, leaf size, and number of shoots. Irradiation dose affected plant height, leaf size, stem diameter and length, and number of leaf. The plant growth increased in conjunction with plant age, but the <br /> higher irradiation dose, the growth of plants slowed down. The best plant <br /> growth was obtained from 5 Gy, not different from control. The highest <br /> rhizome weight (964 g/plant) was obtained from HALINA 1 treated with 10 Gy, differ from control (545 g/plant) and the other treatments.</p><p>Keywords:  Zingiber officinale var. amarum, gamma irradiation (<sup>60</sup>Co), genetic variability, mutation, morphological characters.</p>

EUGENIA ◽  
2013 ◽  
Vol 19 (2) ◽  
Author(s):  
Ventje Pangemanan ◽  
D. S. Runtunuwu ◽  
J. Pongoh

ABSTRACT This study aimed to determine the genetic variability and heritability of morphological characters of some genotypes of potato at the Seed Station in Linelean Village, Modoinding of South Minahasa Regency. The results showed that, the relatively narrow range of genotypes variance for all the characters observed. The coefficient of variance genotypes for plant height, at 14, 42 and 56 DAP (Days After Planting) was relatively narrow, whereas at 7, 21, 28, 35 and 42 DAP relatively wide. For the character of the number of leaves at 7 and 14 DAP are wide and at 21, 28, 35, 42 and 49 DAP relatively narrow. For the character of leaf area at 7 and 14 DAP were wide and at 21, 28, 35, 42 and 49 DAP relatively narrow. For the character of the number of harvest tubers per plant and weight per tuber harvest were wide. Wide sense heritability were high for all the characters observed, except for plant height at 7 and 56 DAP were classified as moderate. Keywords: genotype variance, coefficient variance of genotype and heritability ABSTRAK Penelitian ini bertujuan untuk mengetahui variabilitas genetik dan heritabilitas karakter   morfologis beberapa genotipe kentang di Balai Benih Desa Linelean Modoinding Kabupaten Minahasa Selatan. Hasil penelitian menunjukkan bahwa ragam genotipe tergolong sempit untuk semua karakter yang diamati. Koefisien ragam genotipe untuk karakter tinggi tanaman, pada 14, 42 dan 56 HST (Hari Setelah Tanam)  tergolong sempit, sedangkan pada 7, 21, 28, 35 dan 42 HST tergolong luas. Untuk karakter jumlah daun pada 7 dan 14 HST tergolong luas dan pada 21, 28, 35, 42 dan 49 HST tergolong sempit. Untuk karakter luas daun pada 7 dan 14 HST tergolong luas dan pada 21, 28, 35, 42 dan 49 HST tergolong sempit. Untuk karakter jumlah umbi panen per tanaman dan berat per umbi panen tergolong luas. Heritabilitas arti luas semua karakter tergolong tinggi, kecuali untuk karakter tinggi tanaman pada 7 dan 56 HST yang tergolong sedang. Eugenia Volume 19  No. 2  Agustus 2013 Kata kunci: ragam genotipe, koefisien ragam genotipe dan heritabilitas


2021 ◽  
Vol 13 (1) ◽  
pp. 1
Author(s):  
Vina Eka Aristya

<p class="Abstract" align="center"><strong>ABSTRAK</strong></p><p class="Abstract"><em>Sesamum indicum</em> L. adalah salah satu tanaman alternatif penghasil minyak nabati penting. Pendekatan pemuliaan melalui induksi mutasi berusaha untuk menghasilkan variabilitas baru populasi wijen, yang secara umum tersusun dari individu homozigot. Karakter morfologi berguna untuk mengidentifikasi galur dan memastikan hasil pemuliaan mutasi. Tujuan penelitian ini adalah untuk mengevaluasi keragaman morfologi wijen hasil mutasi berdasarkan 26 karakter kualitatif. Penggalian informasi dengan metode analisis kelompok juga dikaji untuk menggambarkan variabilitas genetik pada 57 galur mutan wijen (jenis hitam dan putih) generasi M4 dan M5. Penelitian ini dilakukan dalam dua tahap: tahap pertama terdiri 18 galur generasi M4, dievaluasi pada Maret-Agustus 2015; tahap kedua terdiri atas 39 galur M5, ditanam pada November 2015 hingga April 2016. Pemeriksaan penampilan kualitatif mengikuti panduan <em>descriptor list</em> untuk wijen. Secara umum, penilaian representatif dari sifat kualitatif pada galur mutan wijen generasi M4 akan diikuti oleh keturunan generasi M5. Dendrogram dibangun untuk membedakan galur menjadi kelompok berdasarkan matriks tingkat kemiripan. Struktur populasi utama dari 57 galur berdasarkan sifat kualitatif dikategorikan dalam dua kelompok besar. Materi genotipe kelompok I diklasifikasikan menjadi dua sub-kelompok, terdiri 17 dan 31 galur. Sub-kelompok ini menjadi bagian distribusi genotipe terbesar. Kelompok II tersusun oleh 9 galur, mayoritas dari M4. Nilai korelasi antar karakter bervariasi antara 0,7176 hingga 1,0. Keragaman morfologi antar galur wijen dipengaruhi oleh sifat genetik dibandingkan faktor lingkungan. Studi ini membantu seleksi galur terpilih berdasarkan kestabilan fenotipe. Evaluasi keragaman struktur populasi wijen mutan bermanfaat untuk program pemuliaan.</p><p class="Abstract" align="center"><strong>ABSTRACT</strong></p><p class="Abstract"><em>Sesamum indicum</em> L. is one of the alternative crops that produces vegetative oil. The plant breeding approach through mutation induction could produce new genetic variability in sesame populations, which are generally composed of homozygous individuals. The study aims were to determine the diversity of sesame-mutant lines based on 26 qualitative characters. Cluster analysis method was carried out to describe the genetic variability of 57 sesame mutant lines (consist of black and white types) 4<sup>th</sup> (M4) and 5<sup>th</sup> generations (M5). The study was done in two phases, the first phases consisted of 18 M4 lines planted in March-August 2015; the second phase consisted 39 M5 lines planted in November 2015 to April 2016. Analyses of qualitative morphological characters followed the descriptors list for sesame. In general, a representative assessment of qualitative traits in M4 lines will be followed by the offspring of M5. Dendrogram showed that the 57 mutant lines categorized into two major clusters. Cluster I were composed of two sub-clusters, consisting 17 and 31 lines. This sub-cluster was the largest part of the genotype distribution. Cluster II composed of 9 lines, where M4 were the majority. The correlation value between characters ranged from 0.7176 up to 1.0. Morphological diversity among lines were largely influenced by genetic rather than environmental factors. This study supports the selection of lines based on phenotype stability. Evaluation of the structural diversity of mutant-sesame populations could be applied in sesame-breeding programs.</p>


2011 ◽  
Vol 32 (No. 2) ◽  
pp. 60-67 ◽  
Author(s):  
S. Shukla ◽  
A. Bhargava ◽  
A. Chatterjee ◽  
A. Srivastava ◽  
S. P Singh

The present investigation was carried out to study different selection parameters for foliage yield and its important yield contributing traits in 29 strains of vegetable amaranth (A. tricolor). The data were recorded for plant height (cm), stem diameter (cm), branches/plant, leaves/plant, leaf size (cm<sup>2</sup>), and protein content (mg/100 mg) in each cutting separately. Foliage yield (kg) was recorded on plot basis comprising 4 cuttings. The highest foliage yield per plot was recorded for strain AV-38, followed by AV-23 and AV-31. In general, protein content was high in the 2<sup>nd</sup> cutting in all strains. The heritability estimates were in general high for all the characters in all the cuttings and ranged from 74.87% to 93.33%. Genetic advance was maximum for foliage yield (42.50%), followed by leaf size (31.02%) and stem diameter (21.13%). It was concluded that foliage yield could be increased substantially in vegetable amaranth through indirect selection based on the characters leaf size and stem diameter. &nbsp;


2018 ◽  
Vol 42 (3) ◽  
Author(s):  
Teguh Wahyono ◽  
Shintia Nugrahini Wahyu Hardani ◽  
Irawans Sugoro

The purpose of this study was to to evaluate the influence of low gamma irradiation dose on growth performance, in vitro gas production and rumen fermentation product of sorghum hydroponic fodder (SHF) to utilize them in ruminant diets. Three polyethylene packages of Samurai 2 sorghum seeds were exposed at 25oC gamma irradiation in gamma cell (Co-60) at doses of 100, 200 and 300 Gy in the presence of air. Samples were then referigated (< 5oC) before planting. All seeds were planted in nutrient film technique hydroponic system. This study used Completely Randomized Design with four replications The observed parameters were total fresh yield, plant height and coversion ratio from seeds to SHF. In vitro gas test evaluation was done to compare all treatments with sorghum straw. The observed parameters were total gas production, kinetics gas, CH4 concentration, CO2 concentration and rumen fermentation products. Results showed that lower irradiation dose for seeds sterilization decreased plant height and total fresh yield on SHF production. In vitro total gas production of all SHF treatment was higher (p<0.05) than sorghum straw (control) at 2-48 h time of incubation. Gamma irradiation dose of 200 and 300 Gy on SHF seeds sterilization decreased (p<0.05) in vitro CH4 concentration for 19.51 and 15.43% respectively compared to SHF control (hypochlorite sterilization). In the same dose, seeds sterilization with gamma irradiation increased (p<0.05) CO2:CH4 ratio by 23.46 and 20.73% respectively compared to SHF control. The treatment of 100 Gy gamma irradiation for seed sterilization also increased (p<0.05) TVFA by 30.63% compared to sorghum straw. It was concluded that lower irradiation dose for seeds sterilization decreased growth performance of SHF. However, 100 Gy gamma irradiation increased in vitro total gas production.


2021 ◽  
Vol 7 (2) ◽  
pp. 47-51
Author(s):  
Lily Marlina ◽  
Joko Purnomo ◽  
Hilda Susanti

This study aimed to: (1) identify the interaction effect between varieties and mixed doses of urea + ZA on the growth and yield of shallots in Ultisol soil; (2) to identify the effect of each shallot varieties and the dosage mixture of urea + ZA which produce the highest yield in Ultisol soils. The research was conducted from April to June 2019 in Tungkaran Village, Martapura, with a split plot design. The main plot was shallot varieties (V) with three levels, namely Biru Lancor (v1), Bima Brebes (v2), and Super Philip (v3). Subplots were a mixture of urea + ZA (P) with four levels, namely 0 kg urea + 600 kg ZA (p1), 100 kg urea + ZA 400 kg (p2), 200 kg urea + 200 kg ZA (p3), and 300 urea kg + ZA 0 kg (p4). Each experiment was repeated three times. Therefore, there were 36 experimental units. Observation parameters included plant height, number of leaves per clump, number of bulbs per clump, fresh bulbs' weight per clump, and yield of stored dry bulbs. The results showed that the Bima Brebes variety (v2) had a significant effect on plant height, number of leaves, number of bulbs per clump, the weight of fresh bulbs per clump, and yield of stored dry bulbs. The mixture of 200 kg urea + 200 g ZA (p3) significantly affected plant height, number of leaves, number of tubers per clump, the weight of fresh tubers per clump, and yield of stored dry tubers.


2021 ◽  
Author(s):  
Ambreena Din ◽  
Zahoor Ahmed Qadri ◽  
MUNEEB AHMAD WANI ◽  
Z.A. Rather ◽  
Shameen Iqbal ◽  
...  

Abstract Chrysanthemum (Dendranthemum grandiflorum kitam.) is amongst the top ten cut flowers globally. The flower colour of ancestral species is restricted to white, yellow, and pink and is acquired from anthocyanins, carotenoids, and the dearth of both pigments, respectively. An extensive array of flower colours, like orange, dark red, purplish-red, and red, has been bred by enhancing the variety of pigments or the amalgamation of both pigments. In recent times, green-flowered cultivars having chlorophylls in their ray petals have been produced and have grown a reputation. Furthermore, violet /blue flowers have been bred via transgenic interventions. Flower colour is considered as critically acclaimed feature of any flower cultivar especially chrysanthemum. Creating newer chrysanthemum cultivars with novel features, for instance, new flower colours in a time and input optimised approach, is the eventual ambition for breeders. Exploring the molecular mechanisms that control flower pigmentation may present imperative suggestions for the rational manoeuvring of flower colour. To generate a diverse array of flower colour mutants in chrysanthemum cv. “Candid” through mutagenesis, in vitro grown micro shoots were exposed to 10, 20, 30, and 40 Gy gamma irradiation at 100 Gy per minute and were evaluated for different parameters. The rhizogenesis parameters declined with the increase in irradiation dose from 0 Gy to 40 Gy, while as, 10 Gy dose proved to record minimum decline in contrast to the control. Survival, leaf size, and the number of leaves plant− 1 after the 8th -week interval also decreased with the increasing trend of gamma irradiation dose but recorded a minimum decline in plants raised from shoots irradiated with 10 Gy gamma irradiation dose with respect to the control. Apparently, the minimum delay in the number of days to floral bud appearance took under 10 Gy compared to control. The highest number of flower colour mutants were recorded under 10 Gy (light pink, orange-pink, white and yellow). Demountable mutation frequency based on flower colour was desirable in plants irradiated with the slightest dose of 10 Gy


2021 ◽  
Author(s):  
Ambreena Din ◽  
Zahoor Ahmed Qadri ◽  
MUNEEB AHMAD WANI ◽  
Z.A. Rather ◽  
Shameen Iqbal ◽  
...  

Abstract In terms of global cut flower production, chrysanthemum (Dendranthemum grandiflorum kitam.) ranks among the top ten. When a plant species possesses colour characteristics associated with those of ancestral species, that colour is described as being white, yellow, or pink, with white coloration representing an absence of pigments, and yellow and pink coloration each represents the presence of carotenoids and anthocyanins, respectively. Pigments, or a combination of pigments, have been improved upon to provide an expansive rainbow of floral colours like orange, dark red, purplish-red, and red. Recent green-flowered varieties have developed a reputation for containing chlorophylls in their ray petals. Additionally, transgenic interventions have been used to breed violet/blue flowers. Flower colour is a highly regarded characteristic of any flower cultivar, particularly chrysanthemum. Breeders' ultimate goal is to create newer chrysanthemum cultivars with innovative characteristics, such as new flower colours, using a time and input-efficient approach. Investigating the molecular mechanisms that regulate flower pigmentation may yield critical insights into the rational manipulation of floral colour. To generate a diverse array of flower colour mutants in chrysanthemum cv. “Candid” through mutagenesis, in vitro grown micro shoots were exposed to 10, 20, 30, and 40 Gy gamma irradiation at 100 Gy per minute and were evaluated for different parameters. The rhizogenesis parameters declined with the increase in irradiation dose from 0 Gy to 40 Gy, while as, 10 Gy dose proved to record minimum decline in contrast to the control. Survival, leaf size, and the number of leaves plant-1 after the 8th-week interval exhibited a downward trend with the increasing trend of gamma irradiation dose but recorded a least drop in plants raised from shoots irradiated with 10 Gy gamma irradiation dose with respect to the control. Apparently, the minimum delay in the number of days to floral bud appearance took under 10 Gy compared to control. The highest number of flower colour mutants was recorded under 10 Gy (light pink, orange-pink, white and yellow). Demountable mutation frequency based on flower colour was desirable in plants irradiated with the slightest dose of 10 Gy.


2015 ◽  
Vol 4 (3) ◽  
pp. 157
Author(s):  
Yusnita Sari ◽  
Ketty Suketi

<p>ABSTRACT</p><p>The objected of this experiment was to know the dose of NPK fertilizer and most appropriate application of GA3  to increase ornamental pepper quality. The experiment has been done from May until August 2009 at Leuwikopo greenhouse, IPB. The experiment was arranged in Split Plot Design within Randomized Complete Block Design. Main Plot factors was application of GA3 (0 ppm, 100 ppm, and 200ppm) and sub plot was dosage of NPK fertilizer (0 g polybag-1, 1.5  g polybag-1, 3 g polybag-1, and 6 g  polybag-1). The results showed that GA3  100 and 200 ppm increased the plant height, number of nodes of plant, and elongated the  internode of stem, but the generative growth of plant become pursued. NPK fertilizer 6 g polybag-1gave the lowest of plant height and have yielded a  few  of  flower  and  fruit.  Based  on  test  of consumer  preferences,  the  best  appearance  was combination without treatment of GA3 and fertilization NPK 3 g polybag-1.</p><p>Key words : Ornamental pepper, GA3, NPK</p><p> </p><p>ABSTRAK</p><p>Tujuan dari penelitian ini adalah untuk mengetahui dosis pupuk NPK dan konsentrasi  yang paling tepat dari aplikasi GA3 untuk meningkatkan kualitas tanaman Lada hias. Percobaan penelitian dilakukan dari bulan Mei sampai dengan Agustus 2009 di rumah kaca kebun percobaan Leuwikopo, IPB. Percobaan menggunakan rancangan Split plot acak lengkap. Plot utama adalah aplikasi GA3 (0, ppm  100  ppm,  dan  200  ppm)  dan  anak  petak  adalah dosis  pupuk  NPK  (0  g  polybag-1,  1.5  g polybag-1, dan 6 g polybag-1). Hasil penelitian menunjukkan bahwa perlakuan GA3 100 dan 200 ppm dapat meningkatkan  tinggi  tanaman,  jumlah  node  dan  internode  pada  caban. Pupuk  NPK  dengan taraf aplikasi 6 g polybag-1 menunjukkan hasil yang terendah pada tinggi tanaman, dan jumlah bunga serta  buah  yang  lebih sedikit.  Berdasarkan  uji  preferensi  konsumen,   yang  paling  disukai adalah Lada dari perlakuan tanpa GA3 dan dengan penambahan pupuk NPK 3 g polybag-1.</p><p>Kata kunci: GA3, NPK, Tanaman Lada NPK</p>


Author(s):  
M Mataa ◽  
B Makungu ◽  
I Siziya

An experiment was conducted to determine the effects of shade on plant development, macronutrient and secondary metabolites in roselle (Hibiscus sabdariffa). Three roselle accessions (ZM 5729, ZM 5738 and ZM ZM5748) acquired from the Zambia National Plant Genetic Resource Centre were used and shade was imposed by intercropping roselle with maize (Zea mays). A Split plot design with shade as main plot and genotype as split- plot was used. Nutritional and development parameters were monitored. At 14 weeks shaded plants had about a three-fold decrease in branches numbers compared to unshaded ones. Plant height was higher in unshaded treatments (120 cm) compared to shaded (83 cm). Plant height of ZM 5738 was significantly higher than the other two accessions. Shading reduced dry matter accumulation by almost 75 % across genotypes with about 20 g per plant in shaded treatments and 73 g in unshaded treatments. Nutrient reductions were highest in fat (60.6 %), fibre (48.4 %), protein (37.8 %), and lowest in ash (27.3 %). In terms of strength of partitioning the pattern was: branches ˃ leaves ˃ roots ˃ squares ˃ flowers. The carbohydrate content increased with shading (10.6 %). Vitamin C content was higher in unshaded treatments in comparison with shaded conditions. ZM 5738 had the highest content followed by ZM 5748 and ZM 5729 had the least content. Oxalates which are an anti- nutrition factor increased with shade. The findings showed that there is significant variation among genotypes and in future selections can be made to choose the most productive genotypes.Int. J. Agril. Res. Innov. & Tech. 8 (1): 7-13, June, 2018


Author(s):  
M. Basnet ◽  
S. M. Shakya ◽  
S. M. Shrestha ◽  
K. Mishra

An experiment was conducted at Sukranagar, Chitwan to assess the effect of nitrogen (N) and off season bulb size on seed production of onion cv. Bemausami-1 (N-8293) during 2009/10. Five levels of nitrogen (0, 40, 80, 120 and 160 kg N/ha) as main plot factor and three bulb size (<1, 1-3 and >3 cm diameter) as sub plot factor were used as treatments and laid out in a split plot design with three replications. Plant height, number of tillers, leaves; scapes, umbels, and flowers per plant were significantly high at higher doses of nitrogen and similar results were found at the bigger sized bulb too. Seed yield was the highest (300.2 kg/ha) at 160 kg N/ha and >3 cm bulb size and the lowest (200.1 kg/ha) at 0 kg N/ha and <1 cm bulb size. B: C ratio was the highest (1.79) at 160 kg N/ha with medium sized bulb (1-3 cm) and the lowest (1.05) at 0 kg N/ha with bulb size of < 1 cm.Journal of the Institute of Agriculture and Animal Science. Vol. 33-34, 2015, Page: 41-46


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