scholarly journals Chicken adaptive response to low energy diet: main role of the hypothalamic lipid metabolism revealed by a phenotypic and multi-tissue transcriptomic approach

2019 ◽  
Author(s):  
Frédéric Jehl ◽  
Colette Désert ◽  
Christophe Klopp ◽  
Marine Brenet ◽  
Andrea Rau ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Adipose Tissue ◽  
Feed Intake ◽  
Egg Production ◽  
Brain Plasticity ◽  
Energy Content ◽  
Low Energy ◽  
Layer Chicken ◽  
Low Energy Diet

Abstract Background Production conditions of layer chicken can vary in terms of temperature or diet energy content compared to the controlled environment where pure-bred selection was undertaken. The aim of this study was to better understand the effect of a 15%-energy depleted diet on egg-production, energy homeostasis and metabolism via a multi-tissue transcriptomic analysis. Study was designed to compare effects of the nutritional intervention in two layer chicken lines divergently selected for residual feed intake. Results Chicken significantly increased their feed intake and decreased their abdominal adipose tissue weight in response to the low-energy diet, whereas their egg-production was unchanged. For each production trait, no significant interaction was observed between diet and line. Moreover, the low energy diet had no effect on adipose tissue and liver transcriptomes. By contrast, the nutritional challenge affected the blood transcriptome and, more severely, the hypothalamus transcriptome which displayed 2700 differentially expressed genes. In this tissue, the low-energy diet lead to an over-expression of genes related to endocannabinoid signaling (CN1R, NAPE-PLD) known to regulate feed intake, and to genes related to polyunsaturated fatty acids synthesis (FADS1, ELOVL5 and FADS2) such as the arachidonic acid which is a precursor of anandamide, a key endocannabinoid. A possible regulatory role of NR1H3 (alias LXRα) has been associated to these transcriptional changes. The low-energy diet further affected brain plasticity-related genes involved in the cholesterol synthesis and in the synaptic activity, revealing a link between nutrition and brain plasticity. It also upregulated genes related to protein synthesis, mitochondrial oxidative phosphorylation and fatty acid oxidation in the hypothalamus, suggesting reorganization in nutrient utilization and biological synthesis in this brain area. Conclusions We observed a complex transcriptome modulation in the hypothalamus of chicken in response to low-energy diet suggesting numerous changes in synaptic plasticity, endocannabinoid regulation, neurotransmission, lipid metabolism, mitochondrial activity and protein synthesis. This global transcriptomic reprogramming could explain the adaptive behavioral response (i.e. increase of feed intake) of the animals to the low-energy content of the diet.

scholarly journals Chicken adaptive response to low energy diet: main role of the hypothalamic lipid metabolism revealed by a phenotypic and multi-tissue transcriptomic approach

2019 ◽  
Author(s):  
Frédéric Jehl ◽  
Colette Désert ◽  
Christophe Klopp ◽  
Marine Brenet ◽  
Andrea Rau ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Adipose Tissue ◽  
Feed Intake ◽  
Egg Production ◽  
Brain Plasticity ◽  
Energy Content ◽  
Low Energy ◽  
Layer Chicken ◽  
Low Energy Diet

Abstract Background Production conditions of layer chicken can vary in terms of temperature or diet energy content compared to the controlled environment where pure-bred selection was undertaken. The aim of this study was to better understand the effect of a 15%-energy depleted diet on egg-production, energy homeostasis and metabolism via a multi-tissue transcriptomic analysis. Study was designed to compare effects of the nutritional intervention in two layer chicken lines divergently selected for residual feed intake. Results Chicken significantly increased their feed intake and decreased their abdominal adipose tissue weight in response to the low-energy diet, whereas their egg-production was unchanged. For each production trait, no significant interaction was observed between diet and line. Moreover, the low energy diet had no effect on adipose tissue and liver transcriptomes. By contrast, the nutritional challenge affected the blood transcriptome and, more severely, the hypothalamus transcriptome which displayed 2700 differentially expressed genes. In this tissue, the low-energy diet lead to an over-expression of genes related to endocannabinoid signaling (CN1R, NAPE-PLD) known to regulate feed intake, and to genes related to polyunsaturated fatty acids synthesis (FADS1, ELOVL5 and FADS2) such as the arachidonic acid which is a precursor of anandamide, a key endocannabinoid. A possible regulatory role of NR1H3 (alias LXRα) has been associated to these transcriptional changes. The low-energy diet further affected brain plasticity-related genes involved in the cholesterol synthesis and in the synaptic activity, revealing a link between nutrition and brain plasticity. It also upregulated genes related to protein synthesis, mitochondrial oxidative phosphorylation and fatty acid oxidation in the hypothalamus, suggesting reorganization in nutrient utilization and biological synthesis in this brain area. Conclusions We observed a complex transcriptome modulation in the hypothalamus of chicken in response to low-energy diet suggesting numerous changes in synaptic plasticity, endocannabinoid regulation, neurotransmission, lipid metabolism, mitochondrial activity and protein synthesis. This global transcriptomic reprogramming could explain the adaptive behavioral response (i.e. increase of feed intake) of the animals to the low-energy content of the diet.

scholarly journals Chicken adaptive response to low energy diet: main role of the hypothalamic lipid metabolism revealed by a phenotypic and multi-tissue transcriptomic approach

2019 ◽  
Author(s):  
Frédéric Jehl ◽  
Colette Désert ◽  
Christophe Klopp ◽  
Marine Brenet ◽  
Andrea Rau ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Adipose Tissue ◽  
Feed Intake ◽  
Egg Production ◽  
Brain Plasticity ◽  
Energy Content ◽  
Low Energy ◽  
Layer Chicken ◽  
Low Energy Diet

Abstract Background Production conditions of layer chicken can vary in terms of temperature or diet energy content compared to the controlled environment where pure-bred selection was undertaken. The aim of this study was to better understand the effect of a 15%-energy depleted diet on egg-production, energy homeostasis and metabolism via a multi-tissue transcriptomic analysis. Study was designed to compare effects of the nutritional intervention in two layer chicken lines divergently selected for residual feed intake. Results Chicken significantly increased their feed intake and decreased their abdominal adipose tissue weight in response to the low-energy diet, whereas their egg-production was unchanged. For each production trait, no significant interaction was observed between diet and line. Moreover, the low energy diet had no effect on adipose tissue and liver transcriptomes. By contrast, the nutritional challenge affected the blood transcriptome and, more severely, the hypothalamus transcriptome which displayed 2700 differentially expressed genes. In this tissue, the low-energy diet lead to an over-expression of genes related to endocannabinoid signaling (CN1R, NAPE-PLD) known to regulate feed intake, and to genes related to polyunsaturated fatty acids synthesis (FADS1, ELOVL5 and FADS2) such as the arachidonic acid which is a precursor of anandamide, a key endocannabinoid. A possible regulatory role of NR1H3 (alias LXRα) has been associated to these transcriptional changes. The low-energy diet further affected brain plasticity-related genes involved in the cholesterol synthesis and in the synaptic activity, revealing a link between nutrition and brain plasticity. It also upregulated genes related to protein synthesis, mitochondrial oxidative phosphorylation and fatty acid oxidation in the hypothalamus, suggesting reorganization in nutrient utilization and biological synthesis in this brain area. Conclusions We observed a complex transcriptome modulation in the hypothalamus of chicken in response to low-energy diet suggesting numerous changes in synaptic plasticity, endocannabinoid regulation, neurotransmission, lipid metabolism, mitochondrial activity and protein synthesis. This global transcriptomic reprogramming could explain the adaptive behavioral response (i.e. increase of feed intake) of the animals to the low-energy content of the diet.

scholarly journals Chicken adaptive response to low energy diet: main role of the hypothalamic lipid metabolism revealed by a phenotypic and multi-tissue transcriptomic approach

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
F. Jehl ◽  
C. Désert ◽  
C. Klopp ◽  
M. Brenet ◽  
A. Rau ◽  
...  
Keyword(s):  
Feed Intake ◽  
Egg Production ◽  
Brain Plasticity ◽  
Energy Content ◽  
Low Energy ◽  
System A ◽  
Layer Chicken ◽  
The Complement System ◽  
Low Energy Diet

Abstract Background Production conditions of layer chicken can vary in terms of temperature or diet energy content compared to the controlled environment where pure-bred selection is undertaken. The aim of this study was to better understand the long-term effects of a 15%-energy depleted diet on egg-production, energy homeostasis and metabolism via a multi-tissue transcriptomic analysis. Study was designed to compare effects of the nutritional intervention in two layer chicken lines divergently selected for residual feed intake. Results Chicken adapted to the diet in terms of production by significantly increasing their feed intake and decreasing their body weight and body fat composition, while their egg production was unchanged. No significant interaction was observed between diet and line for the production traits. The low energy diet had no effect on adipose tissue and liver transcriptomes. By contrast, the nutritional challenge affected the blood transcriptome and, more severely, the hypothalamus transcriptome which displayed 2700 differentially expressed genes. In this tissue, the low-energy diet lead to an over-expression of genes related to endocannabinoid signaling (CN1R, NAPE-PLD) and to the complement system, a part of the immune system, both known to regulate feed intake. Both mechanisms are associated to genes related polyunsaturated fatty acids synthesis (FADS1, ELOVL5 and FADS2), like the arachidonic acid, a precursor of anandamide, a key endocannabinoid, and of prostaglandins, that mediate the regulatory effects of the complement system. A possible regulatory role of NR1H3 (alias LXRα) has been associated to these transcriptional changes. The low-energy diet further affected brain plasticity-related genes involved in the cholesterol synthesis and in the synaptic activity, revealing a link between nutrition and brain plasticity. It upregulated genes related to protein synthesis, mitochondrial oxidative phosphorylation and fatty acid oxidation in the hypothalamus, suggesting reorganization in nutrient utilization and biological synthesis in this brain area. Conclusions We observed a complex transcriptome modulation in the hypothalamus of chicken in response to low-energy diet suggesting numerous changes in synaptic plasticity, endocannabinoid regulation, neurotransmission, lipid metabolism, mitochondrial activity and protein synthesis. This global transcriptomic reprogramming could explain the adaptive behavioral response (i.e. increase of feed intake) of the animals to the low-energy content of the diet.

scholarly journals Xylanase, and the role of digestibility and hindgut fermentation in pigs on energetic differences among high and low energy corn samples1

2019 ◽  
Vol 97 (10) ◽  
pp. 4293-4297 ◽  
Author(s):  
Amy L Petry ◽  
Helen V Masey O’Neill ◽  
John F Patience
Keyword(s):  
Dietary Fiber ◽  
Feed Intake ◽  
Energy Content ◽  
Low Energy ◽  
Total Dietary Fiber ◽  
Fecal Samples ◽  
Ileal Digestibility ◽  
Collection Period ◽  
Dietary Treatments

Abstract The experimental objective was to evaluate the digestibility and fermentation differences between high and low energy corn samples and their response to xylanase supplementation. Four corn samples, 2 with higher DE content (HE-1 and HE-2; 3.74 and 3.75 Mcal DE/kg DM, respectively) and 2 with a lower DE content (LE-1 and LE-2; 3.63 and 3.56 Mcal DE/kg DM, respectively) were selected based upon a previous digestibility trial. Sixteen individually housed barrows (PIC 359 × C29; initial BW = 34.8 ± 0.23kg) were surgically fitted with an ileal T-cannula and randomly allotted to treatments in an 8 × 4 Youden square design. Dietary treatments were arranged in a 4 × 2 factorial: HE-1, HE-2, LE-1, and LE-2, with and without xylanase supplementation. Diets were formulated using one of the 4 corn samples, casein, vitamins, minerals, and 0.4% chromic oxide as an indigestible marker. Feed intake was established at approximately 3 times the estimated energy required for maintenance (NRC 2012) based upon the average initial BW of the pigs at the start of each collection period, which consisted of 9 d adaptation, 2 d of fecal, and 3 d of ileal collections. Diets, ileal, and fecal samples were analyzed for DM, GE, and total dietary fiber (TDF), to determine apparent total tract (ATTD), hindgut fermentation (HF), apparent ileal digestibility (AID) coefficients. A diet × enzyme interaction was not observed for any of the measured variables (P > 0.10). The HE-1 and HE-2 diets had greater ATTD of GE, and HE-2 diet had greater ATTD of DM (P < 0.001 and P = 0.007, respectively). Xylanase, independent of diet, improved the ATTD of GE and DM (84.8 vs. 83.6% for GE with and without enzyme, respectively, P = 0.008; and 84.2 and 83.0% with and without enzyme, respectively, P = 0.007). The energetic differences among these corn samples appeared to be driven by fermentability in the hindgut. Supplementing xylanase improves digestibility irrespective of the digestibility energy content of corn.

scholarly journals Predicting the growth and feed intake of Boer goats in a feedlot system

2020 ◽  
Vol 50 (4) ◽  
pp. 492-500 ◽  
Author(s):  
T.S. Brand ◽  
D.A. Van Der Merwe ◽  
E. Raffrenato ◽  
L.C. Hoffman
Keyword(s):  
Feed Intake ◽  
Linear Models ◽  
Energy Content ◽  
High Energy ◽  
Metabolizable Energy ◽  
Low Energy ◽  
Boer Goat ◽  
Highly Correlated ◽  
Boer Goats ◽  
Low Energy Diet

This research sought to develop a mathematical model to predict the growth and feed intake of Boer goat kids destined for slaughter. Data were collected from castrated kids that were housed in individual pens and fed diets that varied in energy content (11.3, 12.0, and 12.7 MJ metabolizable energy (ME)/kg feed). Growth and feed intake were monitored weekly for 20 weeks, from the time that the kids weighed 22.2 ± 3.5 kg until they were slaughtered at 48.3 ± 0.8 kg. The linear equation (bodyweight = 0.202 age – 5.241 (R2 = 0.84)) was found to predict the growth of kids between 126 and 266 days old. Correlations between expressions of intake and bodyweight were evaluated, cumulative feed intake and bodyweight being most highly correlated (r = 0.93). Linear models were developed to predict cumulative feed intake from bodyweight for goats fed each diet. Regression coefficients were compared between diets, with the slope of the equation for goats on the low energy diet (6.74 ± 0.25) being greater than that of the medium (5.80 ± 0.23) and high energy diets (5.82 ± 0.23). This indicates that goats on the medium and high energy diets gained weight more efficiently than those on the low energy diet. The results from this study can be used by goat producers to predict the growth and intake of goats and determine the ideal feedlot finishing period for optimal productivity.______________________________________________________________________________________Keywords: cumulative intake, dietary energy, modelling

The efficiency of food utilization for egg production by pullets and yearling hens

1959 ◽  
Vol 52 (3) ◽  
pp. 364-368 ◽  
Author(s):  
W. Bolton
Keyword(s):  
Crude Protein ◽  
Egg Production ◽  
High Energy ◽  
Metabolizable Energy ◽  
Low Energy ◽  
Food Utilization ◽  
Digestible Energy ◽  
Digestible Protein ◽  
The Cost ◽  
Low Energy Diet

1. The efficiency of food conversion by pullets and yearlings fed on high- and low-energy diets has been determined.2. Egg production was slightly higher by birds fed the low-energy diet for both pullets and yearling hens; pullets laid more eggs than yearling hens.3. For each age, the gross food intake was greater for birds fed the low-energy diet; the consumptions of digestible protein, non-protein digestible energy and metabolizable energy were the same for both diets and both ages of bird.4. The food cost per dozen eggs was slightly greater in the yearling year when the cost of rearing the pullets was ignored, and about the same when it was included.5. The efficiency of utilization of digestible energy and protein showed only a slight decrease from the pullet to the yearling year.6. The variation in the composition and digestibility of eighteen consecutive mixes of the highenergy diet and twenty-five of the low-energy diet was: crude protein 2%, non-protein digestible energy 5% and metabolizable energy 0·4 kg. cal./g. The digestibility of the crude protein and oil was 87%, for both diets; carbohydrates were 81% digestible in the high-energy diet and 59% in the low-energy diet.

scholarly journals Correlation of Particular Bacterial PCR-Denaturing Gradient Gel Electrophoresis Patterns with Bovine Ruminal Fermentation Parameters and Feed Efficiency Traits

2010 ◽  
Vol 76 (19) ◽  
pp. 6338-6350 ◽  
Author(s):  
Emma Hernandez-Sanabria ◽  
Le Luo Guan ◽  
Laksiri A. Goonewardene ◽  
Meiju Li ◽  
Denis F. Mujibi ◽  
...  
Keyword(s):  
Feed Intake ◽  
Feed Efficiency ◽  
Gel Electrophoresis ◽  
Dry Matter ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Residual Feed Intake ◽  
Low Energy ◽  
Dry Matter Intake ◽  
Gradient Gel Electrophoresis ◽  
Low Energy Diet

ABSTRACT The influence of rumen microbial structure and functions on host physiology remains poorly understood. This study aimed to investigate the interaction between the ruminal microflora and the host by correlating bacterial diversity with fermentation measurements and feed efficiency traits, including dry matter intake, feed conversion ratio, average daily gain, and residual feed intake, using culture-independent methods. Universal bacterial partial 16S rRNA gene products were amplified from ruminal fluid collected from 58 steers raised under a low-energy diet and were subjected to PCR-denaturing gradient gel electrophoresis (DGGE) analysis. Multivariate statistical analysis was used to relate specific PCR-DGGE bands to various feed efficiency traits and metabolites. Analysis of volatile fatty acid profiles showed that butyrate was positively correlated with daily dry matter intake (P < 0.05) and tended to have higher concentration in inefficient animals (P = 0.10), while isovalerate was associated with residual feed intake (P < 0.05). Our results suggest that particular bacteria and their metabolism in the rumen may contribute to differences in host feed efficiency under a low-energy diet. This is the first study correlating PCR-DGGE bands representing specific bacteria to metabolites in the bovine rumen and to host feed efficiency traits.

scholarly journals The effect of different dietary vitamin and mineral levels on certain production parameters, including egg shell characteristics of breeding ostriches

2015 ◽  
Vol 44 (5) ◽  
pp. 51-57
Author(s):  
TS Brand ◽  
GA Tesselaar ◽  
LC Hoffman ◽  
Z Brand
Keyword(s):  
Trace Elements ◽  
Feed Intake ◽  
Egg Production ◽  
Energy Content ◽  
Control Diet ◽  
Specific Pattern ◽  
Dietary Vitamin ◽  
Standard Diet ◽  
Dietary Energy ◽  
Commercial Diet

Earlier studies on breeding birds indicated no effect of dietary energy-content on feed intake. This observation is contradictory to results for other animals and/or poultry where feed intake decreases with an increase in dietary energy level. Literature revealed that a lack of certain nutrients may cause animals to consume more feed than necessary to satisfy their nutrient requirements for these limiting nutrients. In this study eight diet groups were evaluated. A standard commercial diet plus one of the following supplements: (i) Standard diet without a vitamin and mineral premix pack (control diet), (ii) with a normal premix pack, (iii) with normal vitamin and 2 x trace elements (M x 2), (iv) with normal trace elements and 2 x vitamins, (v) with a normal premix pack and limestone added as calcium source; (vi) with a normal premix pack and monocalcium phosphate added as phosphorus source, (vii) with a normal premix pack and soybean oilcake added as crude protein source, (viii) with a normal premix pack and linseed added as a fatty acid source. Significant differences were recorded in the quantity of feed ingested by birds between the diet with surplus minerals (M x 2) (Group 3) (2.3 ± 0.3 kg/bird/day), the diet with no vitamins and minerals added (Group 1) (2.7 ± 0.3 kg/bird/day) and the diet with added fatty acids in the form of linseed (Group 5) (2.9 ± 0.5 kg/bird/day). This indicates the possibility that ostriches may adjust feed intake to satisfy their mineral requirements. No statistically significant relationship was found between the thickness and strength of the shell. Significant differences in shell strength (mean value of 154.7, 109.9, 140.4, 142.7, 153.0, 143.4, 138.4 and 151.1 N/cm2, respectively) were found between all treatments, but no specific pattern could be identified. Results further revealed no effect of dietary treatment on egg production, dead-in-shell eggs (DIS), infertile eggs or chick production. Further experiments that include certain dietary treatments during the five months rest period are currently being done to quantify the effect of the addition of minerals and vitamins during this period on the production of breeding ostriches.Keywords: Eggshell strength; feed intake, minerals, vitamins

scholarly journals High-Energy Diet at Antepartum Decreases Insulin Receptor Gene Expression in Adipose Tissue of Postpartum Dairy Cows

2013 ◽  
Vol 57 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Zhigang Zhang ◽  
Jianguo Wang ◽  
Ruifeng Gao ◽  
Weiqian Zhang ◽  
Xinwei Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Insulin Receptor ◽  
Dairy Cows ◽  
High Energy ◽  
Low Energy ◽  
Control Group ◽  
Insulin Receptor Gene ◽  
Energy Group ◽  
Low Energy Diet

Abstract The objective of the study was to determine expression of gene of insulin receptor (INSR) in adipose tissue of postpartum dairy cows fed diets containing different amounts of energy at the antepartum period. Healthy pregnant dairy cows (n=45) on 21st d of the antepartum were divided into three groups differing in diet composition, namely: control group fed a normal diet, high energy group fed a high energy diet, and low energy group fed a low energy diet. Twenty-one days after parturition, INSR gene expression in adipose tissue was determined by internally controlled reverse transcriptase PCR. The level of INSR mRNA in adipose tissues of cows fed the high energy diet was substantially lower than that in cows fed normal or low energy diets. A relatively higher level of INSR mRNA in the adipose tissue of cows fed low energy diet may be beneficial for gluconeogenesis and lipogenesis, which can relieve an energy negative balance. Reduced level of INSR mRNA in adipose tissue of cows fed high energy diet indicates that the response to insulin has significantly decreased.

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