scholarly journals Microarray expression profile and bioinformatic analyses of circular RNA in human esophageal carcinoma

2019 ◽  
Author(s):  
Xiaohua Chen ◽  
Liping Wang ◽  
Sina Cai ◽  
Xiaona Zhang ◽  
Wenhui Li ◽  
...  
Keyword(s):  
Esophageal Carcinoma ◽  
Therapeutic Targets ◽  
Circular Rna ◽  
Circular Rnas ◽  
Gene Expressions ◽  
Correlation Test ◽  
Spearman Correlation Test ◽  
Receptor Signaling Pathway ◽  
Microarray Expression ◽  
Potential Biomarkers

Abstract Background : Recent studies indicate that noncoding circular RNAs (circRNAs) are involved in the development of esophageal carcinoma. This study aimed to identify circRNAs that are differentially expressed in esophageal carcinoma (EC), which may provide potential biomarkers and therapeutic targets for EC and improve the understanding of its tumorigenesis mechanism. Methods : Ten samples of esophageal carcinoma tissues were sent for circRNA microarray detection. Then the data was subjected to bioimformatic analysis (including circRNA-microRNA (miRNA) coexpression network, Spearman’s correlation test and cancer-related circRNA-miRNA axis analyses). The gene expressions of key circRNAs were detected by real-time- PCR. Results: A total of 102 upregulated and 67 significantly downregulated circRNAs were identified in EC tumors compared to adjacent normal tissue by microarray analysis. One upregulated circRNA (hsa_circRNA_401955) showed the most correlation and was thus regarded as the hub gene by the Spearman correlation test. KEGG pathway enrichment analyses showed that four primary pathways (mRNA surveillance, cytoskeletn actin regulation, spliceosome and the NOD-like receptor signaling pathway) were predicted in the five connected miRNA response elements (MREs) of hub circRNA’ s. Furthermore, cancer-related circRNA-miRNA axis analyses revealed that hsa_circRNA_100375 and its four connected MREs contributed to a cancer-related pathway. The expressions of hsa_circRNA_100375 and hsa_circRNA_401955 were increased significantly in the tumor tissues according to q-PCR. Conclusion: CircRNA dysregulation was involved in the tumorigenesis of EC. The key circRNAs of hsa_circRNA_401955 and hsa_circRNA_100375 may serve as potential biomarkers and therapeutic targets for EC.

scholarly journals Profiling and bioinformatics analyses reveal differential circular RNA expression in NK/T-cell lymphoma-associated hemophagocytic syndrome

2020 ◽  
Vol 40 (9) ◽  
Author(s):  
Changfeng Man ◽  
Yu Fan ◽  
Guangli Yin ◽  
Jiayu Huang ◽  
Jujuan Wang ◽  
...  
Keyword(s):  
T Cell ◽  
Hemophagocytic Syndrome ◽  
Cell Lymphoma ◽  
Gene Prediction ◽  
Therapeutic Targets ◽  
Circular Rna ◽  
T Cell Lymphoma ◽  
Circular Rnas ◽  
Bioinformatics Analyses ◽  
Nk T Cell

Abstract Circular RNAs (circRNAs) may be potential biomarkers or therapeutic targets of hemophagocytic syndrome (HPS) due to their high stability, covalently closed structure and implicated roles in gene regulation. The aim of the present study was to determine and characterize the circRNAs from natural killer (NK)/T-cell lymphoma-associated hemophagocytic syndrome (NK/T-LAHS). CircRNA in NK/T-LAHS and healthy control patient serum were assessed using next-generation sequencing (NGS). One hundred and forty-three differentially expressed circRNAs of which 114 were up-regulated and 29 were down-regulated in NK/T-LAHS patients were identified. Next, Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses to explore the roles of these circRNAs were utilized, and a microRNA (miRNA) target gene prediction software to predict the interaction of circRNAs and miRNAs was used. Moreover, five circRNAs were then selected as NK/T-LAHS candidate circRNAs which were related to tumors and contained NK/T-LAHS-related miRNA-binding sites. Using real-time PCR, the significant up-regulation of these five circRNAs in NK/T-LAHS patient serum were verified. Together these results show that circRNAs may serve as valuable diagnostic biomarkers of early NK/T-LAHS, with potential therapeutic targets in disease progression.

Circular RNA profile of breast cancer brain metastasis: identification of potential biomarkers and therapeutic targets

Epigenomics ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 1619-1630 ◽  
Author(s):  
Bo Fu ◽  
Anqi Zhang ◽  
Mingli Li ◽  
Li Pan ◽  
Wenqiang Tang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Brain Metastasis ◽  
Therapeutic Targets ◽  
Circular Rna ◽  
Breast Cancer Brain Metastasis ◽  
Potential Biomarkers

scholarly journals Circular RNAs in Gastric Cancer: Potential Biomarkers and Therapeutic Targets

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Jiafeng Ouyang ◽  
Zhi Long ◽  
Guoqing Li
Keyword(s):  
Gastric Cancer ◽  
Poor Prognosis ◽  
Noncoding Rnas ◽  
Therapeutic Targets ◽  
Future Research ◽  
Circular Rnas ◽  
Clinical Value ◽  
Treatment Measures ◽  
Potential Biomarkers ◽  
Established Group

Circular RNAs (circRNAs), as a recently established group of endogenous noncoding RNAs, have been involved in the occurrence and development of different malignancies. Gastric cancer (GC) remains a globally significant contributor to death in cancer patients due to insufficient early diagnosis, limited treatment measures, and poor prognosis. An increasing number of studies have found that many circRNAs are dysregulated in GC and are closely associated with its tumorigenesis and metastasis. Thus, circRNAs have the potential to serve as diagnostic and prognostic biomarkers and even therapeutic targets. This review comprehensively summarizes the most recent findings on how circRNAs influence GC progression and their clinical value. In addition, we present several methological deficiencies in the studies and provide some promising ideas for future research.

scholarly journals Cancer-related circular RNA: diverse biological functions

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dan Cheng ◽  
Jing Wang ◽  
Zigang Dong ◽  
Xiang Li
Keyword(s):  
Noncoding Rnas ◽  
Circular Rna ◽  
Long Noncoding Rnas ◽  
Circular Rnas ◽  
Biological Functions ◽  
Diagnosis Of Cancer ◽  
The Relationship ◽  
Potential Biomarkers

AbstractNoncoding RNAs, including long noncoding RNAs (lncRNAs), microRNAs (miRNAs) and circular RNAs (circRNAs), are involved in regulating biological functions. In recent decades, miRNAs and lncRNAs have both inspired a wave of research, but the study of circRNA functions is still in its infancy. Studies have found that circRNAs actively participate in the occurrence and development of various diseases, which emphasizes the importance of circRNAs. Here, we review the features and classification of circRNAs and summarize their functions. Then, we briefly describe how to analyze circRNAs by bioinformatics procedures. In addition, the relationship between circRNAs and cancers is discussed with an emphasis on proving whether circRNAs can be potential biomarkers for the prognosis and diagnosis of cancer.

scholarly journals Circular RNAs: Novel Players in the Oxidative Stress-Mediated Pathologies, Biomarkers, and Therapeutic Targets

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Yunguang Wang ◽  
Wenfang He ◽  
Sherif A. Ibrahim ◽  
Qiang He ◽  
Juan Jin
Keyword(s):  
Oxidative Stress ◽  
Nervous System ◽  
Cardiovascular System ◽  
Significant Role ◽  
Therapeutic Targets ◽  
Circular Rna ◽  
Therapeutic Options ◽  
Circular Rnas ◽  
Comprehensive Overview ◽  
Physiological Conditions

Oxidative stress (OxS) is a wildly described cause of damage to macromolecules, resulting in abnormal physiological conditions. In recent years, a few studies have shown that oxidation/antioxidation imbalance plays a significant role in developing diseases involving different systems and organs. However, the research on the circular RNA (circRNA) roles in OxS is still in its very infancy. Therefore, we hope to provide a comprehensive overview of the recent research that explored the function of circRNAs associated with OxS and its role in the pathogenesis of different diseases that affect different body systems like the nervous system, cardiovascular system, kidneys, and lungs. It provides the possibilities of using these circRNAs as superior diagnostic and therapeutic options for OxS associated with these disease conditions.

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