scholarly journals Isolation of extended spectrum beta-lactamase producing Enterobacteriaceae among children with urinary tract infection: A cross sectional study.

2020 ◽  
Author(s):  
asnakech agegnehu ◽  
Mesfin Worku ◽  
Demiss Nigussie ◽  
Birkneh Tilahun Tadesse ◽  
Birhanu Lulu
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Cross Sectional Study ◽  
Beta Lactamase ◽  
Sectional Study ◽  
Cross Sectional ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Resistant Strains

Abstract Background: Resistance of Gram-negative bacteria to antibiotics had increased at an alarming pace over the last two decades. Empirical antibiotic treatment of UTI practice created opportunity for frequent use of antibiotics that resulted in emergence of resistant strains. Extended spectrum beta-lactamase producing Enterobacteriaceae are emerging worldwide and are usually multi-drug resistant; thus, limiting treatment options for these organisms. Hence, it is mandatory to scrutinize the extent of the problem in the study area. Objective: We investigated the prevalence and correlates of extended spectrum beta-lactamase producing Enterobacteriaceae and their antibacterial resistance profile among children with urinary tract infection (UTI) at Hawassa University Comprehensive Specialized Hospital. Method: A hospital-based cross sectional study was conducted from February 1-July 30/ 2018. A semi-structured questionnaire was used to collect data from 284 children suspected of UTI. Bacterial species were isolated using standard bacteriological techniques. Antibiotic susceptibility testing was done using Kirby-Bauer disk diffusion method and ESBL production was detected by double disc synergy test. Data entry, cleaning and statistical analysis was done using SPSS version 20. Results: Overall, Enterobacteriaceae accounted for 75% (72/96) of UTI infections. The most frequent Enterobacteriaceae were E. coli , 44.4% (32/72) and K. pneumoniae , 27.8% (20/72). Overall, ESBL-producers within Enterobacteriaceae accounted for 41.7% (30/72) and magnitude of multi-drug resistance level was 86.1% (62/72). ESBL producing K. pneumoniae and E. coli accounted for 70% (14/20) and 37.5% (12/32), respectively of all the respective isolates. Previous UTI within the past 12 month (AOR= 0.08, 95% CI (0.01-0.57) and medium family wealth index (AOR = 0.03, 95% CI (0.00-0.27) were associated with infection of ESBL-producing Enterobacteriaceae . Conclusion: ESBL producing Enterobacteriaceae were prevalent among UTI cases; where E. coli and K. pneumoniae were the major isolated resistant strains that contributed mainly to the problem. Majority of Enterobacteriaceae isolated were resistant to commonly prescribed drugs. Key words : ESBL, Antimicrobial Resistance, UTI, Children.

scholarly journals Predicting carriage with extended-spectrum beta-lactamase-producing bacteria at hospital admission: a cross-sectional study

2015 ◽  
Vol 21 (2) ◽  
pp. 141-146 ◽  
Author(s):  
T.N. Platteel ◽  
M.A. Leverstein-van Hall ◽  
J.W. Cohen Stuart ◽  
S.F.T. Thijsen ◽  
E.M. Mascini ◽  
...  
Keyword(s):  
Hospital Admission ◽  
Cross Sectional Study ◽  
Beta Lactamase ◽  
Sectional Study ◽  
Cross Sectional ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

scholarly journals A study on urinary tract infection in a tertiary care hospital

2017 ◽  
Vol 4 (5) ◽  
pp. 1401
Author(s):  
V. Rajendran ◽  
R. Nepoleon ◽  
Prashant V. Solanke ◽  
M. Shahbaz Zailu ◽  
P. Valli
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Oral Antibiotic ◽  
Care Hospital ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
The People

Background: Urinary tract infection is defined as bacteriuria along with urinary symptoms. It is one of the most common bacterial infections in humans and a major cause of morbidity. UTI has become difficult to treat because of appearance of pathogens with increasing resistance to antimicrobial agents. The objective of this study was to determine the bacteriological profile of pathogens responsible for urinary tract infection and to assess the antibiotic sensitivity pattern of the causative uropathogens.Methods: This cross-sectional study was performed at the hospital of Sree Mookambika Institute of Medical Sciences, Kulasekharam during January 1, 2016 to December 12, 2016. Institutional ethical committee clearance was obtained. We surveyed 628 patients, who had clinical manifestations of UTI. Urine specimens were cultured for isolation of the microbial agents of UTI. The isolated bacteria were identified using biochemical tests. Data was entered in Microsoft Excel Version 2016.Statistical analysis was done using SPSS TRIAL VERSION 21C.Results: The commonest organism isolated overall was extended spectrum beta lactamase positive E. coli (35.5%) followed by extended spectrum beta lactamase negative E. coli and Enterococcus. Females (68.63%) were mostly affected than males in our study. The people in the age group of 41-60 years are found to be more (48.46%) affected than the people of other age groups. The most sensitive oral antibiotic to almost all organisms in our study is Nitrofurantoin followed by Cotrimoxazole and Norfloxacin and among parenteral antibiotics, Amikacin stands first followed by Piperacillin-Tazobactam and Gentamicin. Apart from the above antibiotics, Klebsiellapnemoniae also shows good response to Cefotaxime (96.15%) and Cefipime (96.15%) and Aztreonam (92.3%).Conclusions: From our study, it is observed that the most common causative organism for Urinary tract infection is found to be extended spectrum beta lactamase positive E. coli followed by Extended Spectrum Beta Lactamase negative E. coli and Enterococcus. The current status of sensitivity of common organism rests mainly on Nitrofurantoin (oral) and Amikacin, Piperacillin-Tazobactam (parenteral).

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