iTRAQ-based Comparative Proteomic Analysis of Flag Leaves of Two Wheat (Triticum aestivum L.) Genotypes Differing in Waterlogging Tolerance at Anthesis

Abstract Background Waterlogging is one of the major abiotic stresses limiting wheat product. Plants can adapt to waterlogging with changes in morphology, anatomy, and metabolism. A number of genes or proteins were responsive to waterlogging. Results in this sduty, the iTRAQ-based proteomic strategy was applied to identify waterlogging-responsive proteins in wheat. A total of 7710 proteins were identified in waterlogging tolerant and sensitive wheat varieties XM55 and YM158 at anthesis under waterlogging or not. Sixteen proteins were differentially accumulated between XM55 and YM158 under waterlogging with cultivar specificity. Among them, eleven proteins were up-regulated and five proteins were down-regulated. The up-regulated proteins included Fe-S cluster assembly factor, heat shock cognate 70, GTP-binding protein SAR1A-like, and CBS domain-containing protein. The down-regulated proteins contained photosystem II reaction center protein H, carotenoid 9,10 (9',10')-cleavage dioxygenase-like, psbP-like protein 1, and mitochondrial ATPase inhibitor. In addition, nine proteins were responsive to waterlogging with non-cultivar specificity. These proteins included 3-isopropylmalate dehydratase large subunit, solanesyl-diphosphate synthase 2, DEAD-box ATP-dependent RNA helicase 3, and three predicted or uncharacterized proteins. Sixteen out of the twenty-eight selected proteins showed consistent expression patterns between mRNA and protein levels by quantitative real-time PCR. Conclusions: Our study indicates the much proteins were differential accumulated between the two contrast waterlogging wheat varieties in response to waterlogging, which provide insight into wheat response to waterlogging stress. The identified differentially accumulated protein might be applied to develop waterlogging tolerant wheat.

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iTRAQ-based Comparative Proteomic Analysis of Flag Leaves of Two Wheat (Triticum aestivum L.) Genotypes Differing in Waterlogging Tolerance at Anthesis

10.21203/rs.2.9778/v2 ◽

2019 ◽

Author(s):

Mingmei Wei ◽

LiuLong Li ◽

Ke Xie ◽

Rui Yang ◽

Xiaoyan Wang ◽

...

Keyword(s):

Large Subunit ◽

Expression Patterns ◽

Triticum Aestivum L ◽

Mitochondrial Atpase ◽

Cluster Assembly ◽

Atpase Inhibitor ◽

Waterlogging Tolerance ◽

Wheat Varieties ◽

Cbs Domain ◽

Cultivar Specificity

Abstract Background : Waterlogging is one of the major abiotic stresses limiting wheat product. Plants can adapt to waterlogging with changes in morphology, anatomy, and metabolism. Many genes and proteins play critical roles in adaptation to waterlogging. Results : in this study, the iTRAQ-based proteomic strategy was applied to identify the waterlogging-responsive proteins in wheat. A total of 7,710 proteins were identified in two wheat varieties, XM55 (waterlogging-tolerant) and YM158 (waterlogging-sensitive), at anthesis under waterlogging or not. Sixteen proteins were differentially accumulated between XM55 and YM158 under waterlogging with cultivar specificity. Of these, 11 proteins were up-regulated and 5 proteins were down-regulated. The up-regulated proteins included Fe-S cluster assembly factor, heat shock cognate 70, GTP-binding protein SAR1A-like, and CBS domain-containing protein. The down-regulated proteins contained photosystem II reaction center protein H, carotenoid 9,10 (9',10')-cleavage dioxygenase-like, psbP-like protein 1, and mitochondrial ATPase inhibitor. In addition, 9 proteins were responsive to waterlogging with non-cultivar specificity. These proteins included 3-isopropylmalate dehydratase large subunit, solanesyl-diphosphate synthase 2, DEAD-box ATP-dependent RNA helicase 3, and 3 predicted or uncharacterized proteins. Sixteen out of the 28 selected proteins showed consistent expression patterns between mRNA and protein levels. Conclusion s: This study revealed that the proteins were differential accumulated between the two contrast waterlogging wheat varieties in response to waterlogging, which provide valuable insights into wheat response to waterlogging stress. The identified differentially accumulated protein might be applied to develop waterlogging tolerant wheat.

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iTRAQ-based Comparative Proteomic Analysis of Flag Leaves of Two Wheat (Triticum aestivum L.) Genotypes Differing in Waterlogging Tolerance at Anthesis

10.21203/rs.2.9778/v3 ◽

2019 ◽

Author(s):

Mingmei Wei ◽

LiuLong Li ◽

Ke Xie ◽

Rui Yang ◽

Xiaoyan Wang ◽

...

Keyword(s):

Large Subunit ◽

Triticum Aestivum L ◽

Mitochondrial Atpase ◽

Cluster Assembly ◽

Atpase Inhibitor ◽

Waterlogging Tolerance ◽

Wheat Varieties ◽

Cbs Domain ◽

Assembly Factor ◽

Cultivar Specificity

Abstract Background : Waterlogging is one of the major abiotic stresses limiting wheat product. Plants can adapt to waterlogging with changes in morphology, anatomy, and metabolism. Many genes and proteins play critical roles in adaptation to waterlogging. Results : the iTRAQ-based proteomic strategy was applied to identify the waterlogging-responsive proteins in wheat. A total of 7,710 proteins were identified in two wheat varieties, XM55 (waterlogging-tolerant) and YM158 (waterlogging-sensitive), at anthesis under waterlogging or not. Sixteen proteins were differentially accumulated between XM55 and YM158 under waterlogging with cultivar specificity. Of these, 11 proteins were up-regulated and 5 proteins were down-regulated. The up-regulated proteins included Fe-S cluster assembly factor, heat shock cognate 70, GTP-binding protein SAR1A-like, and CBS domain-containing protein. The down-regulated proteins contained photosystem II reaction center protein H, carotenoid 9,10 (9',10')-cleavage dioxygenase-like, psbP-like protein 1, and mitochondrial ATPase inhibitor. In addition, 9 proteins were responsive to waterlogging with non-cultivar specificity. These proteins included 3-isopropylmalate dehydratase large subunit, solanesyl-diphosphate synthase 2, DEAD-box ATP-dependent RNA helicase 3, and 3 predicted or uncharacterized proteins. Conclusion s: This study revealed that the proteins were differential accumulated between the two contrast waterlogging wheat varieties in response to waterlogging, which provide valuable insights into wheat response to waterlogging stress. These differentially accumulated proteins might be applied to develop waterlogging tolerant wheat in further breeding programs.

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iTRAQ-based Comparative Proteomic Analysis of Flag Leaves of Two Wheat (Triticum aestivum L.) Genotypes Differing in Waterlogging Tolerance at Anthesis

10.21203/rs.2.9778/v4 ◽

2020 ◽

Author(s):

Mingmei Wei ◽

LiuLong Li ◽

Ke Xie ◽

Rui Yang ◽

Xiaoyan Wang ◽

...

Keyword(s):

Large Subunit ◽

Triticum Aestivum L ◽

Mitochondrial Atpase ◽

Cluster Assembly ◽

Atpase Inhibitor ◽

Waterlogging Tolerance ◽

Wheat Varieties ◽

Cbs Domain ◽

Assembly Factor ◽

Cultivar Specificity

Abstract Background: Waterlogging is one of the major abiotic stresses limiting wheat product. Plants can adapt to waterlogging with changes in morphology, anatomy, and metabolism. Many genes and proteins play critical roles in adaptation to waterlogging. Results: the iTRAQ-based proteomic strategy was applied to identify the waterlogging-responsive proteins in wheat. A total of 4,999 unique proteins were identified in two wheat varieties, XM55 (waterlogging-tolerant) and YM158 (waterlogging-sensitive), at anthesis under waterlogging or not. Sixteen proteins were differentially accumulated between XM55 and YM158 under waterlogging with cultivar specificity. Of these, 11 proteins were up-regulated and 5 proteins were down-regulated. The up-regulated proteins included Fe-S cluster assembly factor, heat shock cognate 70, GTP-binding protein SAR1A-like, and CBS domain-containing protein. The down-regulated proteins contained photosystem II reaction center protein H, carotenoid 9,10 (9',10')-cleavage dioxygenase-like, psbP-like protein 1, and mitochondrial ATPase inhibitor. In addition, 9 proteins were responsive to waterlogging with non-cultivar specificity. These proteins included 3-isopropylmalate dehydratase large subunit, solanesyl-diphosphate synthase 2, DEAD-box ATP-dependent RNA helicase 3, and 3 predicted or uncharacterized proteins. Conclusions: This study revealed that the proteins were differential accumulated between the two contrast waterlogging wheat varieties in response to waterlogging, which provide valuable insights into wheat response to waterlogging stress. These differentially accumulated proteins might be applied to develop waterlogging tolerant wheat in further breeding programs.

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Genome-wide identification and expression analysis of ADP-ribosylation factors associated with biotic and abiotic stress in wheat (Triticum aestivum L.)

PeerJ ◽

10.7717/peerj.10963 ◽

2021 ◽

Vol 9 ◽

pp. e10963 ◽

Cited By ~ 1

Author(s):

Yaqian Li ◽

Jinghan Song ◽

Guang Zhu ◽

Zehao Hou ◽

Lin Wang ◽

...

Keyword(s):

Triticum Aestivum ◽

Abiotic Stress ◽

Intracellular Transport ◽

Expression Patterns ◽

Functional Characterization ◽

Triticum Aestivum L ◽

Pcr Analysis ◽

Biotic And Abiotic Stress ◽

Specific Expression ◽

Wheat Varieties

The ARF gene family plays important roles in intracellular transport in eukaryotes and is involved in conferring tolerance to biotic and abiotic stresses in plants. To explore the role of these genes in the development of wheat (Triticum aestivum L.), 74 wheat ARF genes (TaARFs; including 18 alternate transcripts) were identified and clustered into seven sub-groups. Phylogenetic analysis revealed that TaARFA1 sub-group genes were strongly conserved. Numerous cis-elements functionally associated with the stress response and hormones were identified in the TaARFA1 sub-group, implying that these TaARFs are induced in response to abiotic and biotic stresses in wheat. According to available transcriptome data and qRT-PCR analysis, the TaARFA1 genes displayed tissue-specific expression patterns and were regulated by biotic stress (powdery mildew and stripe rust) and abiotic stress (cold, heat, ABA, drought and NaCl). Protein interaction network analysis further indicated that TaARFA1 proteins may interact with protein phosphatase 2C (PP2C), which is a key protein in the ABA signaling pathway. This comprehensive analysis will be useful for further functional characterization of TaARF genes and the development of high-quality wheat varieties.

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Characterization of elite bread wheat (Triticum aestivum L.) germplasm for spot blotch Bipolaris sorokiniana (Sacc.) Shoemaker resistance

Plant Genetic Resources ◽

10.1017/s1479262120000490 ◽

2021 ◽

pp. 1-8

Author(s):

Deep Shikha ◽

Chandani Latwal ◽

Elangbam Premabati Devi ◽

Anupama Singh ◽

Pawan K. Singh ◽

...

Keyword(s):

Abiotic Stress Tolerance ◽

Bipolaris Sorokiniana ◽

Triticum Aestivum L ◽

Spot Blotch ◽

Biotic And Abiotic Stress ◽

Wheat Varieties ◽

Effective Measure ◽

Breeding Lines ◽

Elite Breeding Lines ◽

Germplasm Accessions

Abstract Genetic resources are of paramount importance for developing improved crop varieties, particularly for biotic and abiotic stress tolerance. Spot blotch (SB) is a destructive foliar disease of wheat prevalent in warm and humid regions of the world, especially in the eastern parts of South Asia. For the management of this disease, the most effective measure is the development of resistant cultivars. Thus, the present investigation was carried out to confirm SB resistance in 200 germplasm accessions based on phenotypic observations and molecular characterization. These elite breeding lines obtained from the International Centre for Maize and Wheat Improvement, Mexico, are developed deploying multiple parentages. These lines were screened for SB resistance in the field under artificially created epiphytotic conditions during 2014–15 and 2015–16 along with two susceptible checks (CIANO T79 and Sonalika) and two resistant checks (Chirya 3 and Francolin). Eighty-two out of 200 germplasm accessions were found resistant to SB and resistance in these lines was confirmed with a specific SSR marker Xgwm148. Three accessions, VORONA/CNO79, KAUZ*3//DOVE/BUC and JUP/BJY//URES/3/HD2206/HORK//BUC/BUL were observed possessing better resistance than the well-known SB-resistant genotype Chirya3. These newly identified resistant lines could be used by wheat breeders for developing SB-resistant wheat varieties.

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EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)—A New Drought Tolerant Mutant Wheat Line

International Journal of Molecular Sciences ◽

10.3390/ijms22105314 ◽

2021 ◽

Vol 22 (10) ◽

pp. 5314

Author(s):

Marlon-Schylor L. le Roux ◽

Nicolas Francois V. Burger ◽

Maré Vlok ◽

Karl J. Kunert ◽

Christopher A. Cullis ◽

...

Keyword(s):

Amino Acids ◽

Water Deficit ◽

Triticum Aestivum L ◽

Water Deficit Stress ◽

Drought Response ◽

Fibrous Root ◽

Wheat Varieties ◽

Breeding Programs ◽

Response Characteristics ◽

Drought Tolerant

Drought response in wheat is considered a highly complex process, since it is a multigenic trait; nevertheless, breeding programs are continuously searching for new wheat varieties with characteristics for drought tolerance. In a previous study, we demonstrated the effectiveness of a mutant known as RYNO3936 that could survive 14 days without water. In this study, we reveal another mutant known as BIG8-1 that can endure severe water deficit stress (21 days without water) with superior drought response characteristics. Phenotypically, the mutant plants had broader leaves, including a densely packed fibrous root architecture that was not visible in the WT parent plants. During mild (day 7) drought stress, the mutant could maintain its relative water content, chlorophyll content, maximum quantum yield of PSII (Fv/Fm) and stomatal conductance, with no phenotypic symptoms such as wilting or senescence despite a decrease in soil moisture content. It was only during moderate (day 14) and severe (day 21) water deficit stress that a decline in those variables was evident. Furthermore, the mutant plants also displayed a unique preservation of metabolic activity, which was confirmed by assessing the accumulation of free amino acids and increase of antioxidative enzymes (peroxidases and glutathione S-transferase). Proteome reshuffling was also observed, allowing slow degradation of essential proteins such as RuBisCO during water deficit stress. The LC-MS/MS data revealed a high abundance of proteins involved in energy and photosynthesis under well-watered conditions, particularly Serpin-Z2A and Z2B, SGT1 and Calnexin-like protein. However, after 21 days of water stress, the mutants expressed ABC transporter permeases and xylanase inhibitor protein, which are involved in the transport of amino acids and protecting cells, respectively. This study characterizes a new mutant BIG8-1 with drought-tolerant characteristics suited for breeding programs.

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Novel hypergravity treatment enhances root phenotype and positively influences physio-biochemical parameters in bread wheat (Triticum aestivum L.)

Scientific Reports ◽

10.1038/s41598-021-94771-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Basavalingayya K. Swamy ◽

Ravikumar Hosamani ◽

Malarvizhi Sathasivam ◽

S. S. Chandrashekhar ◽

Uday G. Reddy ◽

...

Keyword(s):

Bread Wheat ◽

Vegetative Growth ◽

Growth Parameters ◽

Phenotypic Variability ◽

Crop Improvement ◽

Triticum Aestivum L ◽

Hormonal Changes ◽

Wheat Varieties ◽

Living Organisms ◽

Physiological Benefits

AbstractHypergravity—an evolutionarily novel environment has been exploited to comprehend the response of living organisms including plants in the context of extra-terrestrial applications. Recently, researchers have shown that hypergravity induces desired phenotypic variability in seedlings. In the present study, we tested the utility of hypergravity as a novel tool in inducing reliable phenotype/s for potential terrestrial crop improvement applications. To investigate, bread wheat seeds (UAS-375 genotype) were subjected to hypergravity treatment (10×g for 12, and 24 h), and evaluated for seedling vigor and plant growth parameters in both laboratory and greenhouse conditions. It was also attempted to elucidate the associated biochemical and hormonal changes at different stages of vegetative growth. Resultant data revealed that hypergravity treatment (10×g for 12 h) significantly enhanced root length, root volume, and root biomass in response to hypergravity. The robust seedling growth phenotype may be attributed to increased alpha-amylase and TDH enzyme activities observed in seeds treated with hypergravity. Elevated total chlorophyll content and Rubisco (55 kDa) protein expression across different stages of vegetative growth in response to hypergravity may impart physiological benefits to wheat growth. Further, hypergravity elicited robust endogenous phytohormones dynamics in root signifying altered phenotype/s. Collectively, this study for the first time describes the utility of hypergravity as a novel tool in inducing reliable root phenotype that could be potentially exploited for improving wheat varieties for better water usage management.

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Composition of low-molecular-weight glutenin subunits in common wheat (Triticum aestivum L.) and their effects on the rheological properties of dough

Open Life Sciences ◽

10.1515/biol-2021-0059 ◽

2021 ◽

Vol 16 (1) ◽

pp. 641-652

Author(s):

Sławomir Franaszek ◽

Bolesław Salmanowicz

Keyword(s):

Molecular Weight ◽

Rheological Properties ◽

Common Wheat ◽

High Performance ◽

Triticum Aestivum L ◽

Quality Parameters ◽

Low Molecular Weight ◽

Wheat Varieties ◽

Breeding Lines ◽

Capillary Zone

Abstract The main purpose of this research was the identification and characterization of low-molecular-weight glutenin subunit (LMW-GS) composition in common wheat and the determination of the effect of these proteins on the rheological properties of dough. The use of capillary zone electrophoresis and reverse-phase high-performance liquid chromatography has made it possible to identify four alleles in the Glu-A3 and Glu-D3 loci and seven alleles in the Glu-B3 locus, encoding LMW-GSs in 70 varieties and breeding lines of wheat tested. To determine the technological quality of dough, analyses were performed at the microscale using a TA.XT Plus Texture Analyzer. Wheat varieties containing the Glu-3 loci scheme (Glu-A3b, Glu-A3f at the Glu-A3 locus; Glu-B3a, Glu-B3b, Glu-B3d, Glu-B3h at the Glu-B3 locus; Glu-D3a, Glu-D3c at the Glu-D3 locus) determined the most beneficial quality parameters.

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