scholarly journals Novel ratiometric fluorescent probe based on internal reference and its detection of hydrazine

2021 ◽  
Author(s):  
Xiaohong Cheng ◽  
Shuang Li ◽  
Mengyun Gong ◽  
Song Wang ◽  
Wangnan Li
Keyword(s):  
Cell Imaging ◽  
Fluorescence Enhancement ◽  
Aqueous Media ◽  
Ester Group ◽  
Quantitative Detection ◽  
Blue Fluorescence ◽  
Internal Reference ◽  
Real Water Samples ◽  
Good Solubility ◽  
Sensing System

Abstract In this work, dual-emissive ratiometric fluorescent system was constructed by the introduction of an ideal internal reference. By virtue of its unique alkalinity, N 2 H 4 could undergo a hydrazinolysis reaction with the ester group of F1 , inducing remarkable fluorescence enhancement while the blue fluorescence of the internal reference DPA remained constant. Consequently, the fluorescence intensity ratios (I 540 /I 440 ) were proportional to the concentrations of N 2 H 4 , which was beneficial for the exactly quantitative detection. The skillful strategy granted the sensing system advantages such as relative good solubility in aqueous media, easy-to-design, simple synthesis, large emission shift, good ratiometric response, as well as the successful application in real water samples and cell imaging.

scholarly journals A pH tuning single fluorescent probe based on naphthalene for dual-analytes (Mg2+ and Al3+) and its application in cell imaging

RSC Advances ◽  
2020 ◽  
Vol 10 (36) ◽  
pp. 21399-21405
Author(s):  
Chunwei Yu ◽  
Shuhua Cui ◽  
Yuxiang Ji ◽  
Shaobai Wen ◽  
Li Jian ◽  
...  
Keyword(s):  
Schiff Base ◽  
Fluorescent Probe ◽  
Cell Imaging ◽  
Quantitative Detection

In this study, a naphthalene Schiff-base P which serves as a dual-analyte probe for the quantitative detection of Al3+ and Mg2+ has been designed.

Pillar[5]arene-based supramolecular assemblies with two-step sequential fluorescence enhancement for mitochondria-targeted cell imaging

2020 ◽  
Vol 8 (44) ◽  
pp. 15622-15625
Author(s):  
Hao Guo ◽  
Xin Yan ◽  
Bing Lu ◽  
Jin Wang ◽  
Xiaolei Yuan ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescence Enhancement ◽  
Fluorescence Emission ◽  
Supramolecular Assemblies ◽  
Live Cell ◽  
Supramolecular Nanoparticles

Two-step sequential fluorescence emission enhanced supramolecular nanoparticles are constructed from pillar[5]arene based host–guest interaction and a linear amphiphile. These supramolecular nanoparticles can be applied in mitochondria-targeted live cell imaging.

scholarly journals Green Synthesis of Fluorescent Ag Nanoclusters for Detecting Cu2+ Ions and Its “Switch-On” Sensing Application for GSH

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Yunpeng Shang ◽  
Hui Gao ◽  
Lei Li ◽  
Chaoqun Ma ◽  
Jiao Gu ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Synthesis Method ◽  
Fluorescence Emission ◽  
Emission Peak ◽  
Silver Nanoclusters ◽  
Synthesis Process ◽  
Blue Fluorescence ◽  
Fluorescent Sensing ◽  
Sensing System ◽  
Ag Ncs

Herein, we prepared the L-histidine- (His-) protected silver nanoclusters (Ag NCs) by the microwave synthesis method. The synthesis process was rapid, facile, and environmentally friendly. Under 356 nm excitation, the as-prepared Ag NCs exhibited the blue fluorescence, and the fluorescence emission peak was located at 440 nm. The Ag NCs could successfully detect trace copper (Cu2+) ions in the aqueous solution and the limit of detection (LOD) was as low as 0.6 pM. Interestingly, the Ag NCs showed a different pH-dependent selectivity for both Cu2+ and iron (Fe3+) ions with no responses to other heavy metal ions. Furthermore, the as-fabricated fluorescent sensing system was utilized to detect glutathione (GSH, the LOD was 0.8 nM) by using the “switch-on” fluorescence recovery of Ag NCs through adding glutathione (GSH) to the Cu2+-Ag NCs solution.

Role of Aqueous-Phase Calcination in Synthesis of Ultra-Stable Dye-Embedded Fluorescent Nanoparticles for Cellular Probing

2021 ◽  
pp. 000370282110275
Author(s):  
Rachel Rex ◽  
Soumik Siddhanta ◽  
Ishan Barman
Keyword(s):  
Silica Nanoparticles ◽  
Cell Imaging ◽  
Fluorescent Dyes ◽  
Aqueous Media ◽  
Clinical Diagnostics ◽  
Live Cell ◽  
Metal Nanoparticle ◽  
Superior Performance ◽  
Fluorescent Nanoparticles

Fluorescence imaging is a major driver of discovery in biology, and an invaluable asset in clinical diagnostics. To overcome quenching limitations of conventional fluorescent dyes and further improve intensity, nanoparticle-based constructs have been the subject of intense investigation, and within this realm, dye-doped silica-coated nanoparticles have garnered significant attention. Despite their growing popularity in research, fluorescent silica nanoparticles suffer from a significant flaw. The degradation of these nanoparticles in biological media by hydrolytic dissolution is underreported, leading to serious misinterpretations, and limiting their applicability for live cell and in vivo imaging. Here, the development of an ultra-stable, dye-embedded, silica-coated metal nanoparticle is reported, and its superior performance in long-term live cell imaging is demonstrated. While conventional dye-doped silica nanoparticles begin to degrade within an hour in aqueous media, by leveraging a modified liquid calcination process, this new construct is shown to be stable for at least 24 h. The stability of this metal-enhanced fluorescent probe in biologically relevant temperatures and media, and its demonstrated utility for cell imaging, paves the way for its future adoption in biomedical research.

A novel rhodamine-based fluorescent probe for selective detection of ClO– and its application in living cell imaging

2018 ◽  
Vol 96 (12) ◽  
pp. 1129-1133
Author(s):  
Changping Yang ◽  
Mingliang Zuo ◽  
Xiaoli Hu ◽  
Xuelin Chen ◽  
Duoduo Zhang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Cell Imaging ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Colour Change ◽  
Living Cells ◽  
Fluorescent Imaging ◽  
Selective Detection ◽  
Living Cell Imaging ◽  
Interfering Ions

A novel fluorescent rhodamine-based probe L for selective responding to ClO– has been synthesized and characterized. The spectroscopy showed that probe L can detect ClO– in aqueous solution without interaction with other interfering ions, and the detection is also evident by the colour change from colourless to reddish purple under white light. The remarkable fluorescence enhancement showed the high selectivity and sensitivity of probe L for the detection of ClO–. Furthermore, probe L was applied to intracellular fluorescent imaging of HeLa cells treated with ClO– and MTT assay showed nontoxicity in living cells.

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