scholarly journals In-vitro seed germination Trial for the population Terminalia ivorensis Collected from Benchi-Maji zone, south western Ethiopia

2021 ◽  
Author(s):  
Mohammed Adefa ◽  
Yigardu Mengesha ◽  
Semaigzer Ayalew ◽  
Marshet Nigatu ◽  
Wondu Kebede
Keyword(s):  
Seed Germination ◽  
Natural Conditions ◽  
Running Water ◽  
Treatment Techniques ◽  
Different Types ◽  
Germination Trial ◽  
Tree Seeds ◽  
The One ◽  
Western Ethiopia

Abstract Tree seeds exhibit some degree of dormancy. Similarly, the seeds of Terminalia ivorensis germinate with great difficulties. To break the dormancy and improve the germination capability of seeds, different types of mechanical and physiological approaches can be followed. Accordingly, the seeds of T. ivorensis were subjected to two pre-sowing treatments (one is with seeds soaked in water for 24 hours and the other is with seeds soaked for 10 minutes in 80% concentrated H2SO4 then abundantly rinsed in running water for 20 minutes) in addition to the control one. So, a total of 100 seeds divided into 4 replications (25 seeds per tray) were tested for each treatment. Then, treated seeds were sown in sand tray to seed the germination characteristic of the different treatments. The sown seeds were followed up for 5 months with proper watering, and the germination record was taken accordingly. Analysis of the germination of the different treatments shows that seeds that were not treated exhibits better mean germination percentages (62%) followed by the one with seeds soaked for 24 hours (47%). However, seeds treated with 80% [H2SO4] for 10 minutes then abundantly rinsed in running water for 20 minutes showed no germination. So, both the 62% and 47% germination recorded are fairly accepted results considering the low germination capacity of T. ivorensis in laboratory and natural conditions. However, other possible pre-sowing treatment techniques can be studied and incorporated for increasing the seed germination of T. ivorensis in-vitro in addition to the records obtained in this trial.

scholarly journals Facilitate Seed Germination of the Golden Shower Tree (Cassia fistula) in vitro Using TiO2 Nanoparticles and Scarification Treatments

2016 ◽  
Vol 8 (9) ◽  
pp. 168
Author(s):  
Fatemeh Feizi ◽  
Mousa Mousavi
Keyword(s):  
Seed Germination ◽  
Dry Weight ◽  
Hot Water ◽  
Peat Moss ◽  
Ex Vitro ◽  
Salt Mixture ◽  
Cassia Fistula ◽  
Number Of Leaves ◽  
Tree Seeds

<p>The main propagation method of <em>Cassia fistula</em> is sowing seeds. The seed germination is usually low because of its impermeable hard coat. Therefore, this experiment evaluated the effects of TiO<sub>2</sub> nanoparticles and scarification methods on seed germination and seedling growth <em>in vitro</em> condition. The tree seeds were treated with, hot water, H<sub>2</sub>SO<sub>4</sub> (36N), and mechanical scarification and culture on ¼ MS salt mixture. The medium was supplemented with different concentrations of TiO<sub>2</sub> nanoparticles. The results showed that the highest percentage and rate of germination was recorded in seeds treated with mechanical scarification. The highest shoot and root dry weight was recorded for seeds treated with mechanical scarification and grown on MS media supplemented with 1.5 mg/ml TiO<sub>2</sub> nanoparticles. TiO<sub>2</sub> nanoparticles did not show any significant effects on the percentage and rate of germination. Different growing soil mixtures had significant effects on the growth of the ex vitro transferred plantlets. Coco peat and peat moss mixture (1:1) was found to be more effective in increasing the number of leaves and root length of the seedlings.</p>

scholarly journals Sexual and Asexual Propagation of Teucrium brevifolium

2018 ◽  
Vol 75 (2) ◽  
Author(s):  
Anastasia AKOUMIANAKI–IOANNIDOU ◽  
Aikaterini GERASIMIDOU ◽  
Alexandra SALTA
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Chemical Treatment ◽  
Stem Cuttings ◽  
Asexual Propagation ◽  
Different Types

The propagation with seeds and stem cuttings of Teucrium brevifolium were investigated in order to facilitate the use of the species in floricultural practice and as a medicinal plant. The seeds after they subjected to different types of treatments (dipping in H2SO4 for 15 min or in H2SO4 for 15 min and GA3 1000 mg l-1 for 10 min, 24 or 48h and untreated) cultured for germination in vitro at 5, 10, 15, 20, 25, and 30 °C. The cuttings were planted in a peat/perlite mixture 1:1 v/v in plastic square plug trays in order to study the rooting percentage. Seed germination was very low (2%) at temperatures of 15-25 °C, while chemical treatment with the concentrated H2SO4 for 15 min, increased germination to 8-10% at the same temperatures. Asexual propagation of the species resulted in a 52.5-61.5% rooting percentage, in all seasons except winter. Treatment with IBA 2000 mg l-1 increaseds rooting percentage.

scholarly journals Cytotoxic and Proapoptotic Effects of Resveratrol in In Vitro Studies on Selected Types of Gastrointestinal Cancers

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4350
Author(s):  
Katarzyna Ratajczak ◽  
Sylwia Borska
Keyword(s):  
Multidrug Resistance ◽  
Clinical Medicine ◽  
Pleiotropic Effect ◽  
Anticancer Effect ◽  
Treatment And Prevention ◽  
Different Types ◽  
The One ◽  
Methods Of Treatment

Cancer diseases are currently one of the greatest health challenges in clinical medicine worldwide. Classic methods of treatment often lead to numerous side effects, including the development of multidrug resistance. For this reason, increasing hope is being placed on compounds of natural origin, mainly due to their pleiotropic effect on different types of cells, protective effect on normal cells and toxic effect on cancerous ones. The most studied group are the polyphenolic compounds, which include resveratrol. The effectiveness of polyphenols in the treatment and prevention of many diseases, including cancer of various origins, has become the basis of many scientific studies. The anticancer effect of resveratrol has been demonstrated at all stages of the carcinogenesis process. Additionally, whether administered by itself or in combination with cytostatics, it may play a significant role in the process of reversing multidrug resistance. A review of the effects of resveratrol in in vitro conditions proves that it has a stronger or weaker antiproliferative and proapoptotic effect on the cells of certain neoplasms of the gastrointestinal tract. Despite the differences in the effect of this compound on different types of cancer, a similar tendency can be observed especially regarding the correlation between the concentration of the compound and the incubation time on the one hand and the antitumour effect on the other hand. The information included in this review may prove helpful in planning in vivo and clinical studies in the future.

scholarly journals Establishment of seed in vitro conditions of Swietenia macrophylla to obtain aseptic seedlings

2019 ◽  
pp. 28-31
Author(s):  
Esmeralda J., Cruz-Gutiérrez ◽  
Luis A. Gómez-Reyes ◽  
Jesús Cortes-Aguilar
Keyword(s):  
Seed Germination ◽  
Laminar Flow ◽  
Sodium Hypochlorite ◽  
Economic Importance ◽  
Swietenia Macrophylla ◽  
Important Place ◽  
Running Water ◽  
Laminar Flow Hood ◽  
Seed Disinfection

The mahogany, Swietenia macrophylla, due to its economic importance, occupies an important place within the regional flora of southern and southeastern Mexico. The objective of this work is to obtain a mahogany seed disinfection protocol for its establishment in vitro. The seeds were left in imbibition for 24 hours, then the seed was washed with running water and soap for 3 minutes. Subsequently, within the laminar flow hood, different treatments were carried out, the control 30 min in captan, treatment 1: 30 min in capture 1.5 min in 70% ethanol and 3 min in 30% chlorine. Treatment 2: 30 min in captan 1.5 in 70% ethanol and 4 min in 30% chlorine. Treatment 3: 30 min in captan, 1.5 min in 70% ethanol and 9 min in 30% sodium hypochlorite. The data were subjected to analysis of variance and comparison test of Tukey means (Tukey, α = 0.05). The variables that were evaluated were germination and contamination in the environment. It was observed that there were no significant differences in seed germination. Regarding contamination, treatment three presented the least contamination.

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

Recent Advances in Curcumin Nanocarriers for the Treatment of Different Types of Cancer with Special Emphasis on In Vitro Cytotoxicity and Cellular Uptake Studies

2020 ◽  
Vol 10 (5) ◽  
pp. 577-590
Author(s):  
Jai B. Sharma ◽  
Shailendra Bhatt ◽  
Asmita Sharma ◽  
Manish Kumar
Keyword(s):  
Cancer Cells ◽  
Cellular Uptake ◽  
Anticancer Agents ◽  
Targeted Delivery ◽  
In Vitro Cytotoxicity ◽  
Curcumin Nanoparticles ◽  
Cytotoxicity Studies ◽  
Delivery Technique ◽  
Different Types

Background: The potential use of nanocarriers is being explored rapidly for the targeted delivery of anticancer agents. Curcumin is a natural polyphenolic compound obtained from rhizomes of turmeric, belongs to family Zingiberaceae. It possesses chemopreventive and chemotherapeutic activity with low toxicity in almost all types of cancer. The low solubility and bioavailability of curcumin make it unable to use for the clinical purpose. The necessity of an effective strategy to overcome the limitations of curcumin is responsible for the development of its nanocarriers. Objective: This study is aimed to review the role of curcumin nanocarriers for the treatment of cancer with special emphasis on cellular uptake and in vitro cytotoxicity studies. In addition to this, the effect of various ligand conjugated curcumin nanoparticles on different types of cancer was also studied. Methods: A systematic review was conducted by extensively surfing the PubMed, science direct and other portals to get the latest update on recent development in nanocarriers of curcumin. Results: The current data from recent studies showed that nanocarriers of curcumin resulted in the targeted delivery, higher efficacy, enhanced bioavailability and lower toxicity. The curcumin nanoparticles showed significant inhibitory effects on cancer cells as compared to free curcumin. Conclusion: It can be concluded that bioavailability of curcumin and its cytotoxic effect to cancer cells can be enhanced by the development of curcumin based nanocarriers and it was found to be a potential drug delivery technique for the treatment of cancer.

Seed-borne fungi of cowpea [Vigna unguiculata (L.) Walp] and their possible control in vitro using locally available fungicides in Botswana.

2016 ◽  
Vol 5 (11) ◽  
pp. 5016 ◽  
Author(s):  
K. B. Khare* ◽  
Loeto D. ◽  
Wale K. ◽  
Salani M.
Keyword(s):  
Seed Germination ◽  
Continuous Light ◽  
Sodium Hypochlorite Solution ◽  
Distilled Water ◽  
Percentage Germination ◽  
Department Of Agriculture ◽  
Hypochlorite Solution ◽  
Cowpea Cultivars ◽  
Petri Plate

Seeds of three cowpea cultivars namely Black eye, ER 7 and Tswana obtained from the Department of Agriculture Research, Gaborone were tested for the presence of seed-borne fungi, and their possible control in vitro using locally available fungicides. Four hundred fifty seeds of each cultivar of cowpea were disinfected with 2% sodium hypochlorite solution for 10 min and washed three times with sterile distilled water before placing them in PDA plates (5 seeds/9 cm Petri plate), incubated at 22±2o C for 12 hour each under continuous light and dark. A total of eight fungi were detected from seeds of cowpea. These were Aspergillus flavus, A. niger, Cylindrocarpon sp., Fusarium equisiti, F. oxyaporum, Penicillium chyrosogenum, Rhizopus oligosporus and R. stolonifer. Rhizopus spp. were dominant fungi recovered from seeds, followed by Penicillium, Aspergillus, Fusarium and Cylindrocarpon. The fungi detected resulted in decay and rotting of seeds, and thereby reducing percentage germination of seeds (22%, 37% and 63 % seed germination in Black eye, ER7 and Tswana varieties of cowpea respectively). Out of four fungicides tested, benlate, captan, dithane M 45 and chlorothalanil. Dithane M45 effectively controlled seed-borne fungi, and enhanced seed germination to an average of 86% (93% germination with no fungi detected in Tswana variety) as compared to chlorothalonile (79%), benlate and captan (77%) and un-treated seeds (45%). The fungal incidence was reduced to 2.3%, 4.3%, 5.3% and 5.3% when seeds were treated with dithane M-45, chlorothalonil, benlate and captan respectively as compared to 62% in non-treated seeds.

scholarly journals Phytase-Producing Rahnella aquatilis JZ-GX1 Promotes Seed Germination and Growth in Corn (Zea mays L.)

2021 ◽  
Vol 9 (8) ◽  
pp. 1647
Author(s):  
Gui-E Li ◽  
Wei-Liang Kong ◽  
Xiao-Qin Wu ◽  
Shi-Bo Ma
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Biomass Accumulation ◽  
Root Surface ◽  
Phosphorus Concentration ◽  
Maize Seedlings ◽  
Total Phosphorus Concentration ◽  
Rahnella Aquatilis ◽  
Maize Seeds

Phytase plays an important role in crop seed germination and plant growth. In order to fully understand the plant growth-promoting mechanism by Rahnella aquatilis JZ-GX1，the effect of this strain on germination of maize seeds was determined in vitro, and the colonization of maize root by R. aquatilis JZ-GX1 was observed by scanning electron microscope. Different inoculum concentrations and Phytate-related soil properties were applied to investigate the effect of R. aquatilis JZ-GX1 on the growth of maize seedlings. The results showed that R. aquatilis JZ-GX1 could effectively secrete indole acetic acid and had significantly promoted seed germination and root length of maize. A large number of R. aquatilis JZ-GX1 cells colonized on the root surface, root hair and the root interior of maize. When the inoculation concentration was 107 cfu/mL and the insoluble organophosphorus compound phytate existed in the soil, the net photosynthetic rate, chlorophyll content, phytase activity secreted by roots, total phosphorus concentration and biomass accumulation of maize seedlings were the highest. In contrast, no significant effect of inoculation was found when the total P content was low or when inorganic P was sufficient in the soil. R. aquatilis JZ-GX1 promotes the growth of maize directly by secreting IAA and indirectly by secreting phytase. This work provides beneficial information for the development and application of R. aquatilis JZ-GX1 as a microbial fertilizer in the future.

scholarly journals Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Hongfang Ma ◽  
Rui Li ◽  
Longguang Jiang ◽  
Songlin Qiao ◽  
Xin-xin Chen ◽  
...  
Keyword(s):  
Scavenger Receptor ◽  
Host Cells ◽  
Respiratory Syndrome Virus ◽  
Swine Industry ◽  
Prrs Virus ◽  
Rich Domain ◽  
Serious Disease ◽  
The One ◽  
And Control

AbstractPorcine reproductive and respiratory syndrome (PRRS) is a serious disease burdening global swine industry. Infection by its etiological agent, PRRS virus (PRRSV), shows a highly restricted tropism of host cells and has been demonstrated to be mediated by an essential scavenger receptor (SR) CD163. CD163 fifth SR cysteine-rich domain (SRCR5) is further proven to play a crucial role during viral infection. Despite intense research, the involvement of CD163 SRCR5 in PRRSV infection remains to be elucidated. In the current study, we prepared recombinant monkey CD163 (moCD163) SRCR5 and human CD163-like homolog (hCD163L1) SRCR8, and determined their crystal structures. After comparison with the previously reported crystal structure of porcine CD163 (pCD163) SRCR5, these structures showed almost identical structural folds but significantly different surface electrostatic potentials. Based on these differences, we carried out mutational research to identify that the charged residue at position 534 in association with the one at position 561 were important for PRRSV-2 infection in vitro. Altogether the current work sheds some light on CD163-mediated PRRSV-2 infection and deepens our understanding of the viral pathogenesis, which will provide clues for prevention and control of PRRS.

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