scholarly journals Facilitate Seed Germination of the Golden Shower Tree (Cassia fistula) in vitro Using TiO2 Nanoparticles and Scarification Treatments

2016 ◽  
Vol 8 (9) ◽  
pp. 168
Author(s):  
Fatemeh Feizi ◽  
Mousa Mousavi
Keyword(s):  
Seed Germination ◽  
Dry Weight ◽  
Hot Water ◽  
Peat Moss ◽  
Ex Vitro ◽  
Salt Mixture ◽  
Cassia Fistula ◽  
Number Of Leaves ◽  
Tree Seeds

<p>The main propagation method of <em>Cassia fistula</em> is sowing seeds. The seed germination is usually low because of its impermeable hard coat. Therefore, this experiment evaluated the effects of TiO<sub>2</sub> nanoparticles and scarification methods on seed germination and seedling growth <em>in vitro</em> condition. The tree seeds were treated with, hot water, H<sub>2</sub>SO<sub>4</sub> (36N), and mechanical scarification and culture on ¼ MS salt mixture. The medium was supplemented with different concentrations of TiO<sub>2</sub> nanoparticles. The results showed that the highest percentage and rate of germination was recorded in seeds treated with mechanical scarification. The highest shoot and root dry weight was recorded for seeds treated with mechanical scarification and grown on MS media supplemented with 1.5 mg/ml TiO<sub>2</sub> nanoparticles. TiO<sub>2</sub> nanoparticles did not show any significant effects on the percentage and rate of germination. Different growing soil mixtures had significant effects on the growth of the ex vitro transferred plantlets. Coco peat and peat moss mixture (1:1) was found to be more effective in increasing the number of leaves and root length of the seedlings.</p>

scholarly journals MODIFICAÇÕES NO MEIO DE CULTURA, FOTOPERÍODO E TEMPO DE CULTIVO AFETAM O ALONGAMENTO E ENRAIZAMENTO IN VITRO DE BANANEIRA CV. PACOVAN

Nativa ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 27
Author(s):  
Marcos Vinícius Marques Pinheiro ◽  
Ana Cristina Portugal Pinto De Carvalho ◽  
Fabrina Bolzan Martins
Keyword(s):  
Plant Height ◽  
Culture Medium ◽  
Culture Media ◽  
Dry Weight ◽  
Salt Mixture ◽  
Musa Spp ◽  
Fresh Biomass ◽  
Shoot Dry Weight ◽  
Number Of Leaves

No intuito de elevar as taxas de sobrevivência durante a etapa de aclimatização e posterior plantio a campo, avaliou-se o enraizamento in vitro de bananeira cv. Pacovan, em diferentes concentrações de sais MS e de sacarose. Utilizou-se DIC, esquema fatorial (6x2x3), com seis meios de cultura [sendo três concentrações de nutrientes do meio MS (100%; 50% de macronutrientes; e 50% dos sais macro e micronutrientes), e duas concentrações de sacarose (1,5/3,0%)], dois fotoperíodos (12/16 h) e três tempos de cultivo (21, 28 ou 35 dias) e seis repetições/tratamento. Analisaram-se: altura da planta, número de folhas/planta, massas frescas e secas das partes aérea e radicular. Para altura da planta, massa fresca da parte aérea e radicular, o meio MS 50% dos sais + sacarose (1,5%) com fotoperíodo de 16 h e tempo de cultivo de 35 dias foi satisfatório. Para massa seca da parte aérea foi MS 50% de sais + sacarose (3%), e para massa seca da parte radicular, MS 100% + sacarose (3%) (em 12hs/28 dias e 16hs/21 dias). Para o alongamento/enraizamento in vitro da bananeira cv. Pacovan sugere-se MS 50% de sais (macro e micronutrientes), redução ou manutenção de sacarose (1,5 ou 3%) em 16h/35 dias de cultivo.Palavra-chave: Musa spp., propagação in vitro, sistema radicular. CHANGES IN CULTURE MEDIUM, PHOTOPERIOD AND TIME OF CULTIVATION AFFECT THE IN VITRO ELONGATION AND ROOTING OF BANANA CV. PACOVAN ABSTRACT:In order to achieve high rates of survival during the acclimatization and later planting in the field, was evaluated the in vitro of banana cv. Pacovan plants under different concentrations of sucrose and MS basal salt mixture. The experiment was assembled in a DIC, in 6x2x3, six different culture media [three different MS salt mixture concentrations (100%; 50% of macronutrients; and 50% of macro/micronutrients) and two sucrose concentrations (1.5/3%)], two photoperiods (12/16 hours) and three cultivation times (21, 28 or 35 days). Each treatment was composed by 6 replicates. Plant height, number of leaves/plant, fresh and dry weight of roots and shoots, were analyzed. Satisfactory results for plant height and shoot and root fresh biomass were observed in MS with macro/micronutrients (50%) + sucrose (3%), 16 hours/35 days. The highest values of shoot dry weight were observed in MS with macro/micronutrients (50%) + sucrose (3%); the highest root dry weight was achieved with MS 100% + sucrose (3%) (12hs/28 and 16hs/21 days). The suggested medium for the in vitro elongation and rooting stage of banana cv. Pacovan is the MS with 50% of salts (macro and micronutrients), reduction or maintenance of sucrose (1.5 or 3%) in 16h/35 days of cultivation.Keywords: Musa spp., in vitro propagation, root system. DOI:

scholarly journals Evaluation of allelopathic effect of fifty Sorghum bicolor L. Genotypes on Germination and Growth of some weed in vitro and in vivo

2010 ◽  
Vol 4 (1) ◽  
pp. 95-103
Author(s):  
A. A. Aljibouri ◽  
S. N. Mahmoud ◽  
A. W. Alsawani ◽  
I. S. Alsaadawi ◽  
Duha Mysire Majeed
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Dry Weight ◽  
Amaranthus Retroflexus ◽  
Allelopathic Effect ◽  
Whole Plant ◽  
Number Of Leaves ◽  
Sorghum Genotypes ◽  
Germination And Growth

The effect of root exudates of fifty Sorghum genotypes on seed germination and seedling growth of millet were studied in vitro. The fifty genotypes were divided into four groups according to their effect on percentage reduction in root and shoot and whole plant average lengths of millet seedling growth. The first group caused slight stimulation, the second slight inhibition, the third gave limited inhibition and the fourth caused high inhibition. In seedling growth. Five genotypes of sorghum were selected, one from group two and four from group four. These five genotypes were cultured in the field in Autumn 2007 and at the end of the season plants were removed, dried and grind. Three concentrations of each of these genotypes powder were prepared, in addition to the control treatment, (0.0, 3.0, 6.0 and 9.0) g/kg soil to study their effects on the growth of Amaranthus retroflexus which cultured in pots and kept in experimental field. Data on number of leaves, lengths of shoot and root and whole plant were taken as well as wet and dry weight were measured. Percentage of Amaranthus retroflexus seeds germination were evaluated in an experiment cultured in Petri dishes containing concentrations (0.0, 5.0 and 10.0%) of aqueous extracts of each of the five genotypes powder. Results showed high significant inhibition for the four genotypes from group four in comparison with the one genotype from group two. Significant differences among these genotypes in their inhibition on such parameters as shoot, root, whole plant growth, number of leaves, and wet and dry weight. The genotype 2005-K-Type 1050 gave the highest reduction in plant length 53.2% incomparing with the genotype 2005-K-Type 1033 which gave 0.7%. The percentage of seed germination of Amaranthus retroflexus was significantly reduced as the concentration of the aqueous extract increased and there were significant differences among genotypes on this parameter. The concentration of the powder of each genotype added to the soil produced significant reduction in all parameters studied and the effect increased as the concentration increased in soil. This research discusses the potential of the allelopathic effect of some Sorghum genotypes on weed germination and growth reduction and the possibility of employing them in weed control program with the intention to use less herbicide.

scholarly journals Co-Microencapsulated Black Rice Anthocyanins and Lactic Acid Bacteria: Evidence on Powders Profile and In Vitro Digestion

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2579
Author(s):  
Carmen-Alina Bolea ◽  
Mihaela Cotârleț ◽  
Elena Enachi ◽  
Vasilica Barbu ◽  
Nicoleta Stănciuc
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Oryza Sativa L ◽  
Milk Proteins ◽  
Ethanolic Extract ◽  
Dry Weight ◽  
Hot Water ◽  
Raw Material ◽  
Black Rice

Two multi-functional powders, in terms of anthocyanins from black rice (Oryza sativa L.) and lactic acid bacteria (Lactobacillus paracasei, L. casei 431®) were obtained through co-microencapsulation into a biopolymer matrix composed of milk proteins and inulin. Two extracts were obtained using black rice flour as a raw material and hot water and ethanol as solvents. Both powders (called P1 for aqueous extract and P2 for ethanolic extract) proved to be rich sources of valuable bioactives, with microencapsulation efficiency up to 80%, both for anthocyanins and lactic acid bacteria. A higher content of anthocyanins was found in P1, of 102.91 ± 1.83 mg cyanindin-3-O-glucoside (C3G)/g dry weight (DW) when compared with only 27.60 ± 17.36 mg C3G/g DW in P2. The morphological analysis revealed the presence of large, thin, and fragile structures, with different sizes. A different pattern of gastric digestion was observed, with a highly protective effect of the matrix in P1 and a maximum decrease in anthocyanins of approximatively 44% in P2. In intestinal juice, the anthocyanins decreased significantly in P2, reaching a maximum of 97% at the end of digestion; whereas in P1, more than 45% from the initial anthocyanins content remained in the microparticles. Overall, the short-term storage stability test revealed a release of bioactive from P2 and a decrease in P1. The viable cells of lactic acid bacteria after 21 days of storage reached 7 log colony forming units (CFU)/g DW.

scholarly journals Accelerating Seed Germination and Juvenile Growth of Sorghum (Sorghum bicolor L. Moench) to Manage Climate Variability through Hydro-Priming

Atmosphere ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 419
Author(s):  
Siaka Dembélé ◽  
Robert B. Zougmoré ◽  
Adama Coulibaly ◽  
John P. A. Lamers ◽  
Jonathan P. Tetteh
Keyword(s):  
Seed Germination ◽  
Climate Variability ◽  
Germination Rate ◽  
Dry Weight ◽  
Seed Priming ◽  
Seedling Development ◽  
Hot Water ◽  
Crop Failure ◽  
Simple Method ◽  
Early Season

Agriculture in Mali, a country in Sahelian West Africa, strongly depends on rainfall and concurrently has a low adaptive capacity, making it consequently one of the most vulnerable regions to climate change worldwide. Since early-season drought limits crop germination, and hence growth, ultimately yield during rain-fed depending on production is commonly experienced nowadays in Mali. Germination and establishment of key crops such as the staple sorghum could be improved by seed priming. The effects of hydro-priming with different water sources (e.g., distilled, tap, rain, river, well water) were evaluated respectively for three priming time durations in tepid e.g., at 25 °C (4, 8, and 12 h) and by hot water at 70 °C (in contrast to 10, 20, and 30 min.) in 2014 and 2015. Seed germination and seedling development of nine sorghum genotypes were monitored. Compared to non-primed seed treatments, hydro-priming significantly [p = 0.01] improved final germination percentage, germination rate index, total seedling length, root length, root vigor index, shoot length, and seedling dry weight. The priming with water from wells and rivers resulted in significant higher seed germination (85%) and seedling development, compared to the three other sources of water. Seed germination rate, uniformity, and speed were enhanced by hydro-priming also. It is argued that hydro-priming is a safe and simple method that effectively improve seed germination and seedling development of sorghum. If used in crop fields, the above most promising genotypes may contribute to managing early season drought and avoid failure of seed germination and crop failure in high climate variability contexts.

scholarly journals IDENTIFICATION OF DROUGHT TOLERANT WHEAT GENOTYPES UNDER WATER DEFICIT CONDITIONS

2016 ◽  
Vol 4 (2) ◽  
pp. 206-214 ◽  
Author(s):  
Zaid Chachar ◽  
N. A. Chachar ◽  
Q.I. Chachar ◽  
S.M Mujtaba ◽  
G.A Chachar ◽  
...  
Keyword(s):  
Water Stress ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Root Length ◽  
Dry Weight ◽  
Shoot Length ◽  
Wheat Genotypes ◽  
Maximum Reduction ◽  
Drought Tolerant

Climate change is emerging phenomena and causing frequent drought which lead to scaricity of water, which ultimately nagetively affecting wheat (Triticumaestivum L.) yield all around the world. The aim of this study was to explore the potential deought tolerant wheat genotypes for candidate genes exploration. This study was conducted during the year 2014-2015 at Plant Physiology Division, Nuclear Institute of Agriculture (NIA) Tandojam. The six wheat genotypes (cv. MT-1/13, MT-2/13, MT-3/13, MT-4/13 Chakwal-86 and Khirman) were investigated for their response at germination and seedling stage under different water stress treatments (0, -0.5, -0.75 and -1.0 MPa) in controlled conditions. The results of experiments with reference to genotypes revealed that genotype Chakwal-86 shows maximum seed germination (82.58 %), while the genotype Khirman shows maximum shoot length  (7.23 cm), root length  (15.1 cm), shoot fresh wt. (5.85 g 10-1shoots), root fresh wt.  (3.45 g 10-1roots), shoot dry wt. (1.33 g 10-1shoots), root dry wt. (0.69 g 10-1roots). Among the genotypes tested Khirman and MT-4/13 are the tolerant genotypes had the potential to perform better under drought conditions, whereas  MT-4/13 and Chakwal-86 were moderate tolerant under water stress conditions. Moreover, the genotypes i.e. MT-1/13 and MT-2/13 are the sensitive genotypes under drought environment. It is concluded from present in-vitro studies that osmotic stress significantly reduced the seed germination shoot/root length fresh and dry weight in all six wheat genotypes. The maximum reduction was found at higher osmotic stress induced by PEG-6000 (-1.0 MPa) significantly.

scholarly journals Biofertlizer Lumbrical improves the growth and ex vitro acclimatization of micropropagated pear plants

2021 ◽  
Vol 22 (1) ◽  
pp. 17-30
Author(s):  
Nataliya Dimitrova ◽  
Lilyana Nacheva ◽  
Małgorzata Berova ◽  
Danuta Kulpa
Keyword(s):  
Woody Species ◽  
Photosynthetic Photon Flux Density ◽  
Stem Length ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Ex Vitro ◽  
Fluorescent Lamps ◽  
Planting Material ◽  
Number Of Leaves

In vitro micropropagation of plants is highly useful for obtaining large quantities of planting material with valuable economic qualities. However, plantlets grow in vitro in a specific environment and the adaptation after the transfer to ex vitro conditions is difficult. Therefore, the acclimatization is a key step, which mostly determines the success of micropropagation. The aim of this investigation was to study the effect of the biofertlizer Lumbrical on ex vitro acclimatization of micropropagated pear rootstock OHF 333 (Pyrus communis L.). Micropropagated and rooted plantlets were potted in peat and perlite (2:1) mixture with or without Lumbrical. They were grown in a growth chamber at a temperature of 22&plusmn;2 &deg;C and photoperiod of 16/8 hours supplied by cool-white fluorescent lamps (150 &micro;mol m-2 s-1 Photosynthetic Photon Flux Density, PPFD). The plants were covered with transparent foil to maintain the high humidity, and ten days later, the humidity was gradually decreased. Biometric parameters, anatomic-morphological analyses, net photosynthetic rate and chlorophyll a fluorescence (JIP test) were measured 21 days after transplanting the plants to ex vitro conditions. The obtained results showed that the plants, acclimatized ex vitro in the substrate with Lumbrical, presented better growth (stem length, number of leaves, leaf area and fresh mass) and photosynthetic characteristics as compared to the control plants. This biostimulator could also be used to improve acclimatization in other woody species

scholarly journals ASAI KEMAMPUAN BAKTERI ENDOFIT DARI KACANG TANAH DALAM MENGHAMBAT PERTUMBUHAN Sclerotium sp. PADA KECAMBAH KACANG TANAH

2020 ◽  
Vol 14 (2) ◽  
pp. 178-186
Author(s):  
Lisa Novita Arios ◽  
Dwi Suryanto . ◽  
Kiki Nurtjahja . ◽  
Erman Munir .
Keyword(s):  
Endophytic Bacteria ◽  
Culture Method ◽  
Dry Weight ◽  
Dual Culture ◽  
Seedling Height ◽  
Bacterial Isolates ◽  
Peanut Seed ◽  
Number Of Leaves

Assay on ability of endophytic bacteria isolated from peanut to inhibit Sclerotium sp. growth in peanut seedlings.   A study on assay of ability of endophytic bacteria to inhibit Sclerotium sp. in peanut seedling has been done. The bacteria were isolated from peanut healthy plants, while Sclerotium sp. was isolated from infected peanaut plant. Antagonistic assay was conducted by dual culture method.  In vivo assay of inhibiting Sclerotium sp. was conducted by dipping peanut seed in bacterial solution, and planting the seed in soil:compost (3:1) growing media. Six endophytic bacterial isolates showed to inhibit the growth of Sclerotium sp. in vitro. LN1 seemed to inhibit more of Sclerotium sp., while LN5 showed to inhibit less. Two potential isolates LN1 of gram-negative and LN2 of gram-positive using for further study showed to decrease more of dumping off. It also seemed that the isolates increased the seedling height, number of leaves, and dry weight.

In vitro hardening of red raspberry through CO2 enrichment and relative humidity reduction on sugar-free medium

1993 ◽  
Vol 73 (4) ◽  
pp. 1105-1113 ◽  
Author(s):  
Ribo Deng ◽  
Danielle J. Donnelly
Keyword(s):  
Relative Humidity ◽  
Co2 Enrichment ◽  
Dry Weight ◽  
Early Growth ◽  
Rubus Idaeus ◽  
Ex Vitro ◽  
Red Raspberry ◽  
Plantlet Growth ◽  
Murashige Skoog

Micropropagated shoots of red raspberry (Rubus idaeus L. ’Comet’) were rooted on modified Murashige-Skoog medium lacking sucrose, in specially constructed plexiglass chambers, under ambient (340 ± 20 ppm) or enriched (1500 ± 50 ppm) CO2 and ambient (ca. 100%) or reduced (90 ± 5%) relative humidity. Cultured plantlets were evaluated for their survival, rooting and relative vigor, leaf and root number, stem and root length, total leaf area, total fresh and dry weight, gas exchange rate, and stomatal features, prior to transplantation to soil and at intervals for 6 wk ex vitro. In vitro CO2 enrichment promoted plantlet growth, rooting and both the survival and early growth of transplants. CO2 enrichment increased stomatal aperture of plantlet leaves but did not apparently increase water stress at transplantation. Reduced in vitro RH did not affect plantlet growth but decreased stomatal apertures and stomatal index on leaves of cultured plantlets and promoted both the survival and early growth of transplants. In vitro CO2 and RH levels did not affect the photosynthetic rate of either plantlets or transplants. Only the stomata on leaves of plantlets from the ambient CO2 and reduced RH treatment were functional. Normal stomatal function was not observed in persistent leaves of transplants from the other treatments, even 2 wk after transplantation. In vitro CO2 enrichment acted synergistically with RH reduction in improving growth of plantlets both in vitro and ex vitro. Hardened red raspberry plantlets obtained through CO2 enrichment and RH reduction survived direct transfer to ambient greenhouse conditions without the necessity for specialized ex vitro acclimatization treatment. Key words: Acclimatization, growth analysis, photosynthesis, Rubus idaeus L., stomata, tissue culture

scholarly journals Evaluation of Growth Parameters of Anthurium andreanum Plants Grown in Mixes with Municipal Solid Waste Compost

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 769C-769
Author(s):  
Maria Del Carmen Libran* ◽  
Dania Rivera ◽  
Lizzette Gonzalez
Keyword(s):  
Puerto Rico ◽  
Municipal Solid Waste ◽  
Solid Waste ◽  
Growth Parameters ◽  
Dry Weight ◽  
Peat Moss ◽  
Growth Responses ◽  
Area Index ◽  
Municipal Solid Waste Compost ◽  
Number Of Leaves

In Puerto Rico, the ornamental crop production is one of the most important agricultural enterprises. The growing media most used to grow ornamentals contains peat moss which is very expensive and everyday results less available. There is a need to conduct studies to evaluate alternate organic components. In Puerto Rico, the Municipal solid waste compost (MSW) has been considered as a possible component for a growing mix to grow plants. Results from chemical properties studies of the MSW compost shows that it is a source of nutrients for plants. A raw MSW is a contaminat to the environment, but once is composted does not represent a hazard to humans or environment. The objective of this research was to evaluate the growth responses of Anthurium plants grown in mixes containing different proportions of MSW with a commercial type of mix containing peat moss. Plant of Rosa cultivar Anthura Co. were grown under six treatments containing proportions of MSW: Peatlite mix (0:100,15:85,25:75,50:50,75:25, and 100:0). Data of growth parameters such as number of leaves, leaf area index, clorophyll content, number of flowers, adn fresh and dry weight was gathered and analyzed. Results did show not significant differences in all treatments on number of leaves and leaves area. Clorophyll content was similar on treatment 0:100,15:85 and 25:75 of MSW: Peatlite mix. Fresh and dry weight (g) were lower in all treatments except on 0:100, which got the highest weight. These results shown that MSW could be considered to be a component of a soiless mix to grow ornamentals in order to reduce cost production and environmental impact.

scholarly journals Evaluation of different growth hormones mediated callus induction and regeneration as well as the effect of colchicines, ethyl methanesulfonate (EMS) and gamma radiation on some traits of Impatiens walleriana

Genetika ◽  
2021 ◽  
Vol 53 (1) ◽  
pp. 379-391
Author(s):  
Sedigheh Sharafi ◽  
Pejman Azadi ◽  
Maryam Jafarkhani Kermani ◽  
Vahid Abdossi ◽  
Ali Eskandri
Keyword(s):  
Dry Weight ◽  
Control Treatment ◽  
Lower Survival Rate ◽  
Randomized Design ◽  
Effective System ◽  
Number Of Leaves ◽  
Impatiens Walleriana ◽  
The Impact ◽  
Disease Free

Impatiens is an ornamental member of family Balsaminaceae. This plant mostly propagated by vegetative technique, which generally time wasting process. It is often multiplicities via seed but is barricaded by F1 seed sterility. In vitro culture of Impatiens walleriana has much significant function in fast proliferation with useful features and elicitation of healthful and disease-free plants. This experiment was conducted to investigate the impact of medium and different hormones on in vitro propagation of Impatiens walleriana by using a completely randomized design. MS medium was prepared along with various concentrations of BAP, TDZ and ZEA. Callus was induced and grew well in media supplemented with 0.5 mg/l NAA + 1 mg/l BAP. In order to indirect propagation, explants were cultured in same media containing BAP, ZEA and TDZ in combination with NAA. These treatments have ability to organogenesis. The results revealed that the control treatment had the lowest effect on traits including shoot percentage, number of shoots, number of leaves, shoot length, fresh and dry weight, and it lead to maximum proliferations in medium supplemented with 0.5 mg/l NAA + 1 mg/l BAP. The highest root length and rooting percentage was observed in 0.5 mg/l IBA + 0.5 mg/l BAP. In addition, the effect of mutation agents was studied. Aseptic samples were treated with ?- irradiation, Ethyl Methane Sulfonate and colchicines at growth chamber. Treatments with 30 and 60 grey respectively had the lower survival rate, growth rate and polyploidy while colchicines with 0.1 and 0.2 had the highest rats. Regarding to these, the present technique illustrate an effective system for in vitro reproduction of Impatiens walleriana by hypocotyls cultures. In addition, colchicines proved to be effective in induction of polyploidy in this plantlet.

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