scholarly journals Clone And Function Verification of The OPR Gene Related to Linoleic Acid Synthesis

2021 ◽  
Author(s):  
Min Tan ◽  
Juan Niu ◽  
Duo zi Peng ◽  
Qian Cheng ◽  
Ming Bao Luan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Acid Content ◽  
Rapeseed Oil ◽  
Enrichment Analysis ◽  
Acid Synthesis ◽  
Linoleic Acid Content ◽  
Expression Vectors ◽  
Pathway Enrichment Analysis ◽  
Over Expression

Abstract Background: Fatty acid composition and content affect rapeseed oil quality. Fatty acid synthesis-related genes in rapeseed have been studied globally by researchers. Nevertheless, rapeseed oil is mainly composed of seven different fatty acids, and each fatty acid was regulated by different genes, furthermore different fatty acid contents affect each other, which needs continuous and in-depth research to obtain more clear results.Results: In this paper, broad-scale miRNA expression profiles were constructed and 21 differentially expressed miRNAs were detected. GO enrichment analysis showed that most up-regulated proteins were involved in transcription factor activity and catalytic activity. KEGG pathway enrichment analysis indicated that 20 pathways involving 36 target genes were enriched, of which the bna00592 pathway may be involved in fatty acid metabolism. The results were verified using a Quantitative Real-time PCR (RT-PCR) analysis, and it was found that the target gene of bna-miR156b>c>g was the OPR (12-oxo-phytodienoic acid reductase). Four copies of OPR gene were found, and the over-expression vectors (pCAMBIA1300-35s-OPR and pCAMBIA1300-RNAi-OPR) were constructed to verify their functions. In T1 and T2 plants, OPR-OE (OPR Over-Expression strain) significantly increased linoleic acid content (T1 12.56%, T2 7.185%) and OPRi (OPRi RNA-interference strain) decreased linoleic acid content (T1 5.98%, T2 0.86%).Conclusions: This is the first study to provide four copies of the OPR gene that regulates LA metabolism, can be used for the molecular mechanism of LA and optimizing fatty acid profiles in oilseed for breeding programs.

scholarly journals Abscisic Acid Improves Linoleic Acid Accumulation Possibly by Promoting Expression of EgFAD2 and Other Fatty Acid Biosynthesis Genes in Oil Palm Mesocarp

2021 ◽  
Vol 12 ◽  
Author(s):  
Peng Shi ◽  
Wei Hua ◽  
Yin Min Htwe ◽  
Dapeng Zhang ◽  
Jun Li ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Oil Palm ◽  
Fatty Acid Biosynthesis ◽  
Enrichment Analysis ◽  
Linoleic Acid Content ◽  
Pathway Enrichment Analysis ◽  
Acid Biosynthesis ◽  
Palm Fruit

Abscisic acid plays an important role in fruit development. However, the effect of ABA on fatty acid biosynthesis in oil palm is still unknown. In this study, ABA treatments (CK, A1–A4) were applied to oil palm fruit at 16 WAP (weeks after pollination), and fatty acids in the mesocarp at 24 WAP were analyzed by GC-MS. Results showed that linoleic acid content under treatment A2 (20 μM ABA) was significantly higher (slightly increased by 8.33%) than the control. Therefore, mesocarp samples of A2, and the control at 16, 20, and 24 WAP was sampled for RNA-Seq. KEGG pathway enrichment analysis showed that 43 genes were differentially expressed in the fatty acid biosynthesis pathway, of which expression of EgFAD2 (unigene 105050201) under 20 μM ABA treatment was 1.84-fold higher than in the control at 20 WAP. Further sequence analysis found that unigene 105050201 had more ABA-responsive elements (ABRE), complete conserved domains, and a C-terminal signaling motif among two FAD2 copies. Furthermore, WGCNA and correlation analysis showed co-expression of EgFAD2 (unigene 105050201) with transcription factors (TFs) (WRI1, AP2-EREBP, bZIP, bHLH, C2C2-Dof, MYB, NAC, and WRKY), ABA signaling genes (PYR, PP2C, SnRK, and ABI5), and other genes involved in fatty acid biosynthesis (FATA, FATB, LACS, SAD, Oleosins, and so on). These results indicated that ABA treatment promoted the expression of FAD2 and other genes involved in fatty acid biosynthesis, which possibly resulted in the accumulation of linoleic acid. This study will be helpful for understanding the possible mechanisms through which ABA affects fatty acid biosynthesis and their accumulation in the mesocarp of oil palm.

The effects of probiotics and prebiotics on the fatty acid profile and conjugated linoleic acid content of fermented cow milk

2015 ◽  
Vol 66 (3) ◽  
pp. 254-259 ◽  
Author(s):  
Nadia Manzo ◽  
Fabiana Pizzolongo ◽  
Immacolata Montefusco ◽  
Maria Aponte ◽  
Giuseppe Blaiotta ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Fatty Acid Profile ◽  
Acid Content ◽  
Linoleic Acid Content ◽  
Cow Milk

Effect of seed position on fatty acid content in sunflower (Helianthus annuus L.)

1988 ◽  
Vol 28 (5) ◽  
pp. 629 ◽  
Author(s):  
DL George ◽  
CM McLeod ◽  
BW Simpson
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Palmitic Acid ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Linoleic Acid Content ◽  
Lower Half ◽  
Fatty Acid Level ◽  
Palmitic Acid Content ◽  
Seed Position

Variation among F3 seeds from a single head for linoleic acid content was high (50.0-72.7%) and appeared unrelated to seed position. Fatty acid content of seeds within self-pollinated heads was not affected by unfilled, adjacent seeds. Thus single seed selections for linoleic acid content should not be biased. No general relationship between fatty acid level and seed position was evident from, analyses of bulk samples except that palmitic acid content of seeds from the upper half of heads (7.4%) was higher than from the lower half (6.5%). This may be related to the negative correlation (r = -0.45) between palmitic acid content and seed size. Fatty acid content and weight of seeds from the lower half of heads were less variable than from the upper half.

scholarly journals Formulation of Avocado Juice with Coconut Water Potentially Lowers Hypertension

2018 ◽  
Vol 2 (1) ◽  
pp. 24 ◽  
Author(s):  
Eka Sariningsih ◽  
Mia Srimiati
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Acid Content ◽  
Linoleic Acid Content ◽  
Ash Content ◽  
Coconut Water ◽  
Linolenic Acid Content ◽  
High Potassium ◽  
Total Unsaturated Fatty Acid ◽  
Serving Size

<p><em>Avocado is a fruit that high unsaturated fatty aci</em><em>d, while</em><em> coconut water is fluid </em><em>which has</em><em> high potassium. </em><em>The objective of this study was<strong> </strong>t</em><em>o assess the formulation of avocado juice with coconut water that could potentially reduce hypertension. The design of the study was an experimental with the degree of subsititution of coconut water as treatments. There were 3 kinds of treatment, i.e. F1 (75% avocado and 25% coconut water), F2 (50% avocado and 50% coconut water), F3 (25% avocado and 75% coconut water) and F0 (without coconut water but using water). Avocado juice product with 75% avocado substitution and 25% coconut water (F1) was a best formula determined by the hedonic test. This formula contained 68,48% (%w/w) water, 3,12% (%w/w) ash content, 4,45% (%w/w) fat content, 1,76% (%w/w) protein content, 21,07% (%w/w) carbohydrate content, 2163,8 (mg/kg) potassium content, 9,20% (%w/w) linoleic acid content, 1,11% (%w/w) linolenic acid content and 42,4% (%w/w) of total unsaturated fatty acid. This product could potentially reduce and prevent hypertension, because in one serving size (200cc) contain 432,76 mg of potassium (claim source of potassium). </em><em></em></p>

The trans fatty acid and conjugated linoleic acid content of Fulani butter oil in Nigeria

2006 ◽  
Vol 19 (6-7) ◽  
pp. 704-710 ◽  
Author(s):  
R.H. Glew ◽  
J.H. Herbein ◽  
I. Ma ◽  
M. Obadofin ◽  
W.A. Wark ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Trans Fatty Acid ◽  
Acid Content ◽  
Linoleic Acid Content ◽  
Butter Oil

scholarly journals Changes in fatty acid composition of pork rich in conjugated linoleic acid frying in different kind of fats

2009 ◽  
pp. 31-35
Author(s):  
Anikó Borosné Győri ◽  
Istvánné Hermán ◽  
János Gundel ◽  
János Csapó
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Palmitic Acid ◽  
Acid Composition ◽  
Sunflower Oil ◽  
Acid Content ◽  
Linoleic Acid Content ◽  
Palmitic Acid Content ◽  
High Level

In 1990ys antiatherogen, antioxidant and anticarcinogen effect of conjugated linolacids (CLA) was detected. From this reasons, our aims in this study were producing pork rich in CLA and studying the change of fatty acid composition of the produced pork cooked different kind of fats. For frying palm and sunflower oil and swine fat were used. Thigh was cutted for 100 g pieces. Meat pieces were fried at 160 °C for 1 and 8 minutes. Estimation of frying data it was determined that higher (0.13%) CLA content of pork was spoiled (60-70%) except in case of swine fat cooking,because it is extremly sensitive for oxidation and heating. Swine fat has higher (0.09%) CLA content than plant oil, protecting the meat’s original CLA content. Cooking in swine fat did not have significant effect on fatty acid composition of meat. Low level of palmitic acid contect of sunflower oil (6.40%) decreased for half part of palmitic acid content of pork (24.13%) and it produced cooked meat with decreased oil acid content. Contrary of above, linoleic acid content of fried meat was increased in different folds as compared to crude pork. If it was fried in sunflower oil with high level linoleic acid increased (51.52%) the linoleic acid content in fried pork. The linoleic acid content of the high level CLA pork increased four times (48.59%) to the crude meat (16.59% and 12.32%). The high palmitic acid content of palm fat (41.54%) increased by 60% the palmitic acid content in fried pork, low stearic acid (4.44%) and linoleic acid content (10.56%) decreased the stearic and linoleic acid content of crude meat.

scholarly journals PHYSIOLOGICAL CHANGES ASSOCIATED WITH HARDCORE DEVELOPMENT IN SWEETPOTATOES

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 490d-490
Author(s):  
Tonda Bardwell ◽  
Huang Jin Xing ◽  
J. O. Garner ◽  
J. L. Silva
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Linoleic Acid Content ◽  
Physiological Changes ◽  
Total Lipids ◽  
Storage Roots

Storage roots of `Jewel', `Centennial' and `Beauregard' were chilled at 5C for 0, 10, 20, 30 or 40 days. After chilling, the roots were placed at 21C for two days to allow hardcore development. Hardcore was measured as weight of root that remain hard after boiling for 45 minutes. Hardcore and fatty acid composition of total lipids were compared for the three cultivars. Hardcore was present at 10 days in both `Jewel ' and `Centennial' and at 20 days for `Beauregard'. Severity of hardcore increased with time of chilling. Linoleic acid content of 'Beauregard ' was higher for the 0, 10, and 20 day sampling periods, and decreased to a level equal to that found in 'Jewel ' and `Centennial'.

scholarly journals Fatty Acid Composition and Conjugated Linoleic Acid Content in Different Carcass parts of Dağlıç Lambs

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Ali Karabacak ◽  
İbrahim Aytekin ◽  
Saim Boztepe
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Linoleic Acid Content

This study was conducted to compare fatty acid composition and content of conjugated linoleic acid (CLA) in different regions of sheep carcasses. Lambs of the Dağlıç breed were used for this purpose. Subsequent to a 68-day period of intensive fattening, fatty acids were examined in samples taken from the legs, shoulders, breasts, and ribs of lamb carcasses. According to the analysis, in leg, shoulder, breast, and rib, respectively, total saturated fatty acids (SFA) were found to be 40.38, 42.69, 42.56, and 40.27%, unsaturated fatty acids (MUFA) were found to be 40.38, 44.17, 46.17, and 49.50%, polyunsaturated fatty acids (PUFA) were found to be 4.79, 4.29, 3.80, and 3.72%, and CLAs were found to be 1.49, 1.69, 1.53, and 1.59%.

Sign in / Sign up

Export Citation Format

Share Document