scholarly journals Detection and Identification of Potentially Infectious Gastrointestinal and Respiratory Viruses at Workplaces of Wastewater Treatment Plants With Viability qPCR/RT-qPCR

Author(s):  
Agata Stobnicka-Kupiec ◽  
Małgorzata Gołofit-Szymczak ◽  
Marcin Cyprowski ◽  
Rafał L. Górny

Abstract This study aimed to qualitatively and quantitatively assess the prevalence of the most common respiratory and gastrointestinal viruses in the air, surface swab, and influent/effluent samples collected in wastewater treatment plants (WWTPs). Application of qPCR/RT-qPCR assays combined with PMA dye pretreatment allowed detecting the potentially infectious and disintegrated viral particles in collected samples. In the air at workplaces in WWTPs, the most frequent isolation with the highest concentrations (reaching up to 103 gc/m3 of potentially infectious intact viral particles) were observed in case of adenoviruses (AdVs) and rotaviruses (RoVs), followed by noroviruses (NoVs). Viruses were significantly more often detected in the air samples collected with Coriolis®μ impinger, than with MAS-100NT impactor. The temperature negatively (Spearman correlation: –1 < R < 0; p < 0.05), while RH positively (0 < R < 1; p < 0.05) affected airborne concentrations of potentially infectious viral particles. In turn, the predominant viruses on studied surfaces were RoVs and NoV GII with concentrations of potentially infectious virions up to 104 gc/100 cm2. In the cases of SARS-CoV-2 and presumptive SARS-CoV-2 or other coronaviruses, their concentrations reached up to 103 gc/100 cm2. The contamination level of steel surfaces in WWTPs was similar to this on plastic ones. This study revealed that the qualitative and quantitative characteristics of respiratory and gastrointestinal viruses at workplaces in WWTPs is important for proper exposure assessment and needs to be included in risk management in occupational environment with high abundance of microbial pollutants derived from wastewater.

Author(s):  
A. Plakhova ◽  
K. Plemyashov

In these studies, a comprehensive assessment of the effect of the drugs «Carofertin» and «Hemobalance» on the reproductive function of oocyte donor cows was carried out. At the same time, the effect of these drugs on the biochemical parameters of blood serum, as well as on the concentration of progesterone, carotene in the experimental groups and the activity of the ovaries in the experimental animals was assessed. An increase in the activity of alkaline phosphatase in the first and third experimental groups was found, which amounted to 128.80 and 104.6 IU / L. There was also a decrease in the concentration of cholesterol in the experimental groups compared with the control. The maximum concentration of carotene was noted in the experimental group No. 1, which were injected with «Carofertin» subcutaneously, and in the experimental group No. 3, which were injected with «Carofertin» and «Hemobalance». The maximum concentration of progesterone was also noted in groups No. 3 and No. 1 and amounted to 21.98±3.03 and 17.01±9.68 nmol / l, respectively. In the course of ultrasound examination of the ovaries in experimental animals, a relationship was noted between the concentration of progesterone and the intensity of the blood supply of the corpus luteum on the ovaries in experimental animals. Subsequently, in one animal from each experimental group, oocytes were aspirated (OPU) twice with an interval of two weeks and their qualitative and quantitative characteristics were assessed. The maximum number of oocytes suitable for use was noted in the experimental group No. 3, which was injected with both study drugs.


2020 ◽  
pp. 39-47
Author(s):  
Irina Muntyan

The work model of a nurse includes 14 elements representing the qualitative and quantitative characteristics of the work. The analysis of these elements allowed us to form and offer work models for senior and rankand-file nurses. These models ensure selection, evaluation and placement of nursing personnel on a uniform methodological basis.


1983 ◽  
Vol 14 (3) ◽  
pp. 155-166 ◽  
Author(s):  
Wm. Hogland ◽  
R. Berndtsson

The paper deals with the qualitative and quantitative characteristics of urban discharge. Ratios for urban discharge and recipient flow during different time intervals are presented and discussed. The quality of the urban discharge is illustrated through pollutographs.


Author(s):  
Sara Gholipour P ◽  
Sara Minagar ◽  
Javad Kazemitabar ◽  
Mobin Alizadeh

Background: A novel type of control strategy is presented for control of chaotic systems particularly a chaotic robot in joint and workspace which is the result of applying fractional calculus to dynamic sliding mode control. Objectives: To guarantee the sliding mode condition, control law is introduced based on the Lyapunov stability theory. Methods: A control scheme is proposed for reducing the chattering problem in finite time tracking and robust in presence of system matched disturbances. Conclusion: Also, all of chaotic robot's qualitative and quantitative characteristics have been investigated. Numerical simulations indicate viability of our control method. Results: Qualitative and quantitative characteristics of the chaotic robot are all proven to be viable thru simulations.


2021 ◽  
pp. 18-24
Author(s):  
А.Н. Гайфуллин

Социально-экономическое развитие есть планомерная процедура совершенствования уровня жизни, качественных и количественных характеристик, потребляемых общественностью благ. Исходя из ситуации со здоровьем населения в регионе, выставляются соответствующие ориентиры. В разрезе региональной специфики закладываются и реализуются целевые программы, которые одновременно зависят от здоровья населения и направлены, собственно, на улучшение качества его здоровья. Согласно тематике настоящей статьи, ведущий фактор социально-экономического роста - здоровье населения, именно он будет оценен как катализатор искомого регионального развития. Socio-economic development is a systematic procedure for improving the standard of living, qualitative and quantitative characteristics of the goods consumed by the public. Based on the health situation of the population in the region, appropriate guidelines are set. In the context of regional specifics, targeted programs are being laid and implemented, which at the same time depend on the health of the population and are aimed, in fact, at improving the quality of its health. According to the subject of this article, the leading factor of socio-economic growth is the health of the population, it will be evaluated as a catalyst for the desired regional development.


2021 ◽  
Vol 1 (38) ◽  
pp. 8-13
Author(s):  
M. G. Soykher ◽  
A. V. Lepilin ◽  
M. I. Soykher ◽  
I. K. Pisarenko ◽  
G. T. Saleeva ◽  
...  

The temporomandibular joint is paired, a complex formation of an ellipsoid shape, which is formed by the articular head of the lower jaw, the mandibular fossa and the articular tubercle of the temporal bone, covered with fibrous cartilage. There are two types of movements in the temporomandibular joint: translation and rotation, which implement protrusion-retrusion, right and left mediotrusion, and opening-closing. Computerized axiography is used for assessment of the mandibular movements and the patient’s skeletal parameters. This type of examination allows you to adjust the articulator for an individual function and to study the qualitative and quantitative characteristics of the temporomandibular joint.


Author(s):  
Iryna Pashko ◽  

The complex structure of the institution of public service is defined as a set of individual institutions, which organizationally and conceptually determine its qualitative and quantitative characteristics. It is shown that the nature of the processes taking place in the institute of civil service of Ukraine is determined by external volitional management decisions, the purpose of which is the implementation of urgent socially significant goals, which do not have a direct impact on the institute of civil service. The indirect influence of public administration on the institution of public service is indicated. In particular, the author emphasizes the decisive role of political processes that form the conceptual directions of the state's development and determine the priorities of management activities. The influence of actions on the Ukraine-EU association on the processes of institutionalization of the civil service is shown. A striking example of the synergistic effect of complex management decisions is actions aimed at eliminating the informal destructive institution of corruption. This prompts the search for and application of a new scientific and theoretical toolkit and methodological apparatus for studying the processes of institutionalization in the postmodern synergetic paradigm. In turn, this contributes to the qualitative analysis and forecasting of the prospects for the development of the civil service; will provide a scientifically grounded choice from among possible alternatives to modernize the research institute and will guide the practical development of targeted comprehensive programs and strategies for managing changes in its structure. Thus, taking into account the influence of complex bifurcation processes in public administration at the present stage will make it possible to identify trends in the institutionalization of public service in new qualitative and quantitative characteristics.


Author(s):  
Juliana S. Zeymer ◽  
Paulo C. Corrêa ◽  
Gabriel H. H. de Oliveira ◽  
Fernanda M. Baptestini ◽  
Rita C. P. Freitas

ABSTRACT Lactuca sativa seeds are highly sensitive to climate conditions; thus, they should be stored securely to maintain their qualitative and quantitative characteristics. Studies on hygroscopicity aim to decrease possible changes in agricultural products under specific environmental conditions. Accordingly, this study aims to develop an appropriate mathematical model to represent the desorption isotherms of Lactuca sativa seeds. The hygroscopic equilibrium was achieved using a static-gravimetric method at temperatures of 10, 20, 30, 40 and 50 °C and water activity in the range 0.11-0.96. Six mathematical models were fitted to the experimental data of the equilibrium moisture content of Lactuca sativa seeds. The best model was chosen based on the determination coefficient (R2), magnitude of mean relative error (MRE), standard deviation of the estimate (SDE), and analysis of residue distribution. The modified Oswin model best represented the hygroscopicity of the Lactuca sativa seeds, with values of 8.02% and 0.55 for the MRE and SDE, respectively; moreover, the residual values were randomly distributed. The shape of the isotherms of the Lactuca sativa seeds estimated using the modified Oswin model is sigmoidal, which is characteristic of a type II curve.


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