Hyperbaric Oxygen Combined with Alginate Oligosaccharide Delay D-gal-induced Liver, Kidney and Spleen Senescence in Mice by Attenuating Oxidative Stress
Abstract Objectives Hyperbaric oxygen treatment (HBOT) is an adjuvant therapy progressively applied in the treatment of clinical diseases, and alginate oligosaccharide (AOS) is a natural polysaccharide with excellent antioxidant property. In this study, the effects of HBOT combined with AOS on the senescence on liver, kidney and spleen were investigated in a D-galactose (D-gal) -induced senescent mice model. Results The findings indicated that HBOT combined with AOS could delay D-gal-induced aging of liver, kidney and spleen in mice. Specifically, HBOT combined with AOS improved the daily behavior and organ indices of mice. Biochemical and morphological analyses of all tissues indicated that HBOT combined with AOS markedly decreased the unusual elevation of ALT, AST and CREA induced by D-gal, alleviated pathological injury, and significantly enhanced the capacity of antioxidant defense system of mice, including increaing T-SOD, CAT AND GSH-Px activities and decreasing MDA content . In addition, Western Blot results indicated that HBOT combined with AOS also inhibited the overexpression of aging-related proteins p53 and p16. Conclusions To some extent, these findings suggest that HBOT combined with AOS has significant anti-senescence potential in D-gal-induced senescent mice, and the underlying factors may be correlated with the enhancement of antioxidant defense capacity.