Amelioration of oxidative stress-mediated cytotoxicity and genotoxicity induced by copper and flubendiamide in-vivo and in- vitro by potent antioxidants
Abstract Present study was designed to assess the toxicity of copper @ 33 mg/kg and flubendiamide @ 200 mg/kg in vivo in male Wistar rats orally once daily for 90 days and protective effect of α-tocopherol, resveratrol, curcumin and catechin and in vitro cyto-genotoxicity in primary cell culture of thymocytes. In vivo study showed significant (p<0.05) increase in AST, total bilirubin and uric acid, creatinine and BUN levels while decrease in total proteins, GSH, SOD and GST levels and increased LPO and GPx with severe degenerative changes were observed in liver and kidney tissues in intoxicated groups. In vitro thymocytes were exposed to 40 µM concentration of flubendiamide and/or showed significant increase in TUNEL+ve cells, micronuclei, DNA shearing, and comet formation per 100 cells. Concurrent treatment with α-tocopherol in xenobiotics intoxicated groups showed almost normal values of the biochemical parameters and decreased LPO production and improved antioxidant enzymes activities and histoarchitecture of liver and kidney tissues suggest ameliorative potential of α-tocopherol whereas, resveratrol, curcumin, catechin or α-tocopherol in vitro decreased TUNEL+ve cells, micronuclei induction and comet formation and effect of antioxidants was concentration-dependent and their order of potency on equimolar concentration (10 µM) basis is: curcumin > resveratrol >catechin = α-tocopherol.