scholarly journals Transcriptomic Analysis of Fertilization Effects on the Biosynthesis of Sesquiterpenes in Essential Oils from Phoebe Bournei Twigs and Leaves

2021 ◽  
Author(s):  
Li Liu ◽  
Gongxiu He ◽  
Xu Wang ◽  
Dangquan Zhang
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Plant Biomass ◽  
Raw Material ◽  
Compound Fertilizer ◽  
Expression Of Genes ◽  
Leaf Essential Oil ◽  
Main Components ◽  
Fertilization Effects ◽  
Phoebe Bournei

Abstract Background: Phoebe bournei is a potential medicinal plant whose essential oil (EO) from leaves has potential inhibitory activities against some bacterium, tumor, and has a certain potential for hypoglycemic activity. Fertilization is a common and effective method to increase plant biomass, which can increase the raw material of essential oil, but has a certain impact on the composition and biological activity of plant essential oil. Results: The main components are sesquiterpenes in the essential oils from leaves and twigs. The yield of the essential oils and the content of their main components can be modulated by compost and compound fertilizer, to different degrees, and minor differences were registered among the categories of the components in essential oils. However, changes were strongly mirrored in some main components of essential oils. The content of the primary (+) - calarene in the leaf EO were strongly increased by compost, but the opposite happened by compound fertilizer. On the contrary, the effect of compound fertilizer was more significant on the main components of twig essential oil than compost. The transcriptome sequencing results of P. bournei showed that the total number of DEGs in twigs and leaves treated with compost were significantly more than that with compound fertilizer. No change was found in the expression of genes regulating principal components. However, the expression of several key genes regulating the upstream substrates for the synthesis of the sesquiterpenes was significantly changed: the expression of two key speed limiting enzymes genes (DXS and HMGR) and two important branch-point enzyme genes (FPPS and GGPPS) was significantly down regulated, while the expression of gene (HMGS) was significantly up-regulated.Conclusion: The expression levels of genes (DXS2, HMGR, FPPS and GGPPS) were significantly down regulated in leaves treated with compost, resulting in the changes of the yield and main components of the leaf essential oil. The effect of compost was more significant on the synthesis of the essential oil from P. bournei leaves than that of compound fertilizer.

scholarly journals COMPONENT COMPOSITION OF ESSENTIAL OIL PRUNUS PERSICA VAR. NECTARINA GROWING IN UZBEKI-STAN

2020 ◽  
pp. 165-170
Author(s):  
Ra"no Botirovna Karabaeva ◽  
Alidzhan Aminovich Ibragimov ◽  
Otabek Mamadaliyevich Nazarov
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Prunus Persica ◽  
Component Composition ◽  
Raw Material ◽  
Oil Composition ◽  
Pale Yellow ◽  
Yellow Color ◽  
The Republic ◽  
Main Components

The article presents the results of determining the composition of essential oils of Prunus persica var. nectarina varieties "yellow nectarine" – "sariк luchchak (uzb.) ", growing in two regions of the Ferghana region of the Republic of Uzbekistan. The pale yellow essential oil was obtained by hydrodistillation from un-dried fresh leaves of plants. The oil composition was determined by chromatography-mass spectrometry on an Agilent 7890 AGC 6890 N gas chromotograph with a quadrupole mass spectrometer (Agilent 5975C inert MSD) as a detector on an HP-5 MS quartz capillary column. An essential oil of pale yellow color was isolated from the leaves of plants. In the composition of essential oils, 56 and 61 compounds were identified in the first and second samples, respectively, wich is 94.55 and 96.00% of the total components. The dominant components of the first saple are camphor bicyclic monoterpene ketones (24.21%), α-thujone (15.00%) and β-thujone (4.27%), aromatic aldehyde benzaldehyde (18.83%) and isobornoleol bicyclic monoterpene alcohol (6.17%). In the second sample, bicyclic monoterpenic ketones (camphor) (36.67%), α-thujone (21.81%) and β-thujone (7.06%) and bicyclic monoterpene alcohol isobornoleol (9.4%) predominate and monocyclic unsaturated monoterpene α-terpinene (2.18%). In both samples, (+)-2-bornanone (camphor) is predominant. The studied variety Prunus persica var. nectarina can serve as a raw material for the production of essential oil, the main components of which are camphor and isobornoleol.

Chemical Compositions of the Essential Oils and Headspace Volatiles of Seseli peucedanoides Plant Parts

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1985069
Author(s):  
Goran M. Petrović ◽  
Jelena G. Stamenković ◽  
Olga P. Jovanović ◽  
Gordana S. Stojanović
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Chemical Compositions ◽  
Static Headspace ◽  
Headspace Volatiles ◽  
Plant Parts ◽  
Major Class ◽  
Germacrene D ◽  
Leaf Essential Oil ◽  
Main Components

The chemical compositions of the essential oils of Seseli peucedanoides (M.Bieb.) Koso-Pol. inflorescences and leaves, isolated by hydrodistillation, and headspace volatiles, obtained by the static headspace method, were analyzed in detail by gas chromatography (GC) and GC/mass spectrometry (MS). In total, 74 constituents were identified, representing more than 98% of the observed GC peaks. The number of identified essential oil components obtained from the inflorescences was 63 while for the leaf essential oil it was 46. A much smaller number of compounds, 26 for inflorescences and 21 for leaves, were detected in the headspace samples. In both essential oils the most abundant compounds were the same, ( E)-caryophyllene and germacrene D, only in different proportions. The main components in the headspace specimens were α-pinene and ( E)-β-ocimene with ( E)-caryophyllene and ( Z)-3-hexen-1-ol also determined in significant percentages. The major class of compounds identified in the investigated essential oils was hydrocarbon sesquiterpenes with a share of over 80%, while the most dominant class of the headspace volatiles was hydrocarbon monoterpenes, which contribute slightly less than 80% of the total.

scholarly journals Chemical Composition, Antibacterial, and Anti-Inflammatory Activities of Essential Oils from Flower, Leaf, and Stem of Rhynchanthus beesianus

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Qi Chen ◽  
Xiaoge Zhao ◽  
Tingya Lu ◽  
Yao Yang ◽  
Yi Hong ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Chemical Constituents ◽  
Bornyl Acetate ◽  
Edible Plant ◽  
Leaf Essential Oil ◽  
Application Potential ◽  
Anti Inflammatory ◽  
Main Components ◽  
Factor Α

Rhynchanthus beesianus is a medicinal, ornamental, and edible plant, and its essential oil has been used as an aromatic stomachic in China. In this study, the chemical constituents, antibacterial, and anti-inflammatory properties of flower essential oil (F-EO), leaf essential oil (L-EO), and stem essential oil (S-EO) of R. beesianus were investigated for the first time. According to the GC-FID/MS assay, the F-EO was mainly composed of bornyl formate (21.7%), 1,8-cineole (21.6%), borneol (9.7%), methyleugenol (7.7%), β-myrcene (5.4%), limonene (4.7%), camphene (4.5%), linalool (3.4%), and α-pinene (3.1%). The predominant components of L-EO were bornyl formate (33.9%), borneol (13.2%), 1,8-cineole (12.1%), methyleugenol (8.0%), camphene (7.8%), bornyl acetate (6.2%), and α-pinene (4.3%). The main components of S-EO were borneol (22.5%), 1,8-cineole (21.3%), methyleugenol (14.6%), bornyl formate (11.6%), and bornyl acetate (3.9%). For the bioactivities, the F-EO, L-EO, and S-EO exhibited significant antibacterial property against Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa, and Escherichia coli with the inhibition zones (7.28–9.69 mm), MIC (3.13–12.50 mg/mL), and MBC (6.25–12.50 mg/mL). Besides, the F-EO, L-EO, and S-EO significantly inhibited the production of proinflammatory mediator nitric oxide (NO) (93.15–94.72%) and cytokines interleukin-6 (IL-6) (23.99–77.81%) and tumor necrosis factor-α (TNF-α) (17.69–24.93%) in LPS-stimulated RAW264.7 cells at the dose of 128 μg/mL in the absence of cytotoxicity. Hence, the essential oils of R. beesianus flower, leaf, and stem could be used as natural antibacterial and anti-inflammatory agents with a high application potential in the pharmaceutical and cosmetic fields.

scholarly journals Composition and Antimicrobial and Anti-wood-decay Fungal Activities of the Leaf Essential Oils of Machilus pseudolongifolia from Taiwan

2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500
Author(s):  
Chen-Lung Ho ◽  
Pei-Chun Liao ◽  
Kuang-Ping Hsu ◽  
Eugene I-Chen Wang ◽  
Wei-Chih Dong ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Wood Decay ◽  
Sesquiterpene Hydrocarbons ◽  
Leaf Oil ◽  
Leaf Essential Oil ◽  
Main Components

The hydrodistillated leaf essential oil of Machilus pseudolongifolia was analyzed to determine its composition and yield. Seventy compounds were identified, the main components being β-eudesmol (26.8%), α-cadinol (20.8%), viridiflorene (8.9%), α-caryophyllene (5.3%), globulol (4.6%) and β-caryophyllene (4.2%). Oxygenated sesquiterpenes (60.1%) and sesquiterpene hydrocarbons (31.4%) were the predominant groups of compounds. The leaf oil exhibited excellent antimicrobial and anti-wood-decay fungal activities.

scholarly journals Antioxidant and Cytotoxic Properties of Essential Oils from Native Brazilian Lauraceae Species

2020 ◽  
pp. 1-8
Author(s):  
Fabiana Lima Silva ◽  
Amanda Espírito Santo ◽  
Paola Cristina Branco ◽  
Letícia Veras Costa-Lotufo ◽  
Maria Claúdia Marx Young ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Human Tumor ◽  
Cancer Cell Line ◽  
Major Constituent ◽  
Leaf Essential Oil ◽  
Hct 116 ◽  
Main Components

Analysis of the leaf and stem essential oils from three Brazilian Lauraceae species showed that the major constituent of O. odorifera was safrole (57.1 and 88.5% for leaves and stems, respectively) while for O. indecora leaves the major ones were the α-pinene (12.8%) and β-pinene (12.4%) andβ-bisabolol (12.2%) in the stems. For the third species, Persea venosa, the main components of leaf and stem oils were spathulenol (27.8 and 14.7%, respectively) and humulene epoxide II (11.3 and 5.1%, respectively). The bioactivity results indicated that the leaf essential oil from O. indecora was the most active for both radical scavenging activity (IC50=0.142mg/mL) and cytotoxicity against human tumor cells, with growth inhibitions for the colon tumoral line HCT-116 (100%)and breast cancer cell line MCF-7 (99.2%), at 5 μg/mL concentration. This activity might be related to the presence ofα-pinene and β-pinene in the leaf essential oil of O. indecora. However, further studies with the isolated compounds are necessary to fully understand these bioactivites.

scholarly journals COMPOSITION OF ESSENTIAL OIL OF ARTEMISIA MACROCEPHALA JACQUE EX BESSER GROWING IN MONGO-LIA

2019 ◽  
pp. 105-112
Author(s):  
Svetlana Vasil'yevna Zhigzhitzhapova ◽  
Sambuunyam Sambuunyam ◽  
Tuyana Erdemovna Randalova ◽  
Larisa Dorzhiyevna Radnaeva
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Raw Material ◽  
Quadrupole Mass ◽  
Mass Selective Detector ◽  
Aerial Parts ◽  
Selective Detector ◽  
Main Components ◽  
Derivatives Of ◽  
Dark Blue

Сomposition of the essential oils of the plant Artemisia macrocephala, growштп in the central (aimak Arkhangai), northeastern (aimak Khentii) parts of Mongolia and compared with literature data are presented  in this paper. Essential oil was obtained by hydrodistillation method from dried aerial parts of plants.  Gas chromatography (GC) analyses was performed on an Agilent Technologies 6890 gas chromatograph equipped with quadrupole mass selective detector HP 5973  (MS) and an HP-5MS capillary column (30 m × 0.25 mm × 0.2 μm).  Dark-blue essential oil was isolated from the aerial part of the plant.  Chamazulene (7.4-16.1%) and α-bisabolol (3.4-20.7) are  main components of essential oil of  Artemisia macrocephala , are which indicates the promise of using this species  as a raw material for the isolation of essential oil with anti-inflammatory, bactericidal , regenerative properties. Oil are characterized by a significant content  (sum 20–28.9%) of derivatives of nerol (neryl-2-methylbutanoate, neryl-3-methylbutanoate, nerylpentanoat) and its isomer of  geraniol (geranyl-2-methylbutanoate, geranyl-3-methylbutanoate). Six compounds (1,8-cineol, terpineol-4, α-terpineol, β-celinene, T-cadinol, methyl isocostate) are also constant for the studied samples. Comparison with the literature data shows that the compositions of essential oils of Artemisia macrocephala of  mongolian and russian (siberian) flora are close.

scholarly journals Chemical Compositions, Mosquito Larvicidal and Antimicrobial Activities of Essential Oils from Five Species of Cinnamomum Growing Wild in North Central Vietnam

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1303 ◽  
Author(s):  
Do N. Dai ◽  
Nguyen T. Chung ◽  
Le T. Huong ◽  
Nguyen H. Hung ◽  
Dao T.M. Chau ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Larvicidal Activity ◽  
Antimicrobial Agents ◽  
Chemical Compositions ◽  
North Central ◽  
Mosquito Larvicidal Activity ◽  
Central Vietnam ◽  
Leaf Essential Oil

Members of the genus Cinnamomum (Lauraceae) have aromatic volatiles in their leaves and bark and some species are commercially important herbs and spices. In this work, the essential oils from five species of Cinnamomum (C. damhaensis, C. longipetiolatum, C. ovatum, C. polyadelphum and C. tonkinense) growing wild in north central Vietnam were obtained by hydrodistillation, analyzed by gas chromatography and screened for antimicrobial and mosquito larvicidal activity. The leaf essential oil of C. tonkinense, rich in β-phellandrene (23.1%) and linalool (32.2%), showed excellent antimicrobial activity (MIC of 32 μg/mL against Enterococcus faecalis and Candida albicans) and larvicidal activity (24 h LC50 of 17.4 μg/mL on Aedes aegypti and 14.1 μg/mL against Culex quinquefasciatus). Cinnamomum polyadelphum leaf essential oil also showed notable antimicrobial activity against Gram-positive bacteria and mosquito larvicidal activity, attributable to relatively high concentrations of neral (11.7%) and geranial (16.6%). Thus, members of the genus Cinnamomum from Vietnam have shown promise as antimicrobial agents and as potential vector control agents for mosquitoes.

scholarly journals Determination of Essential Oil Bioactive Components and Rosmarinic Acid of Salvia officinalis Cultivated under Different Intra-row Spacing

2013 ◽  
Vol 5 (2) ◽  
pp. 198-203 ◽  
Author(s):  
Mohammad ABU DARWISH ◽  
Ezz Al-Dein AL-RAMAMNEH ◽  
Ivan SALAMON ◽  
Ziad ABU-DIEYEH ◽  
Mohamed AL NAWAISEH ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinic Acid ◽  
Plant Density ◽  
Salvia Officinalis ◽  
Raw Material ◽  
Row Spacing ◽  
Human Disorders ◽  
Tea Production ◽  
First Time

Salvia officinalis, known also as sage, is a medicinal plant belonging to the Lamiaceae family that spreads all over the word in several countries. The demand for the raw material and extracts of this plant is increasing due to its numerous applications in pharmacy, food and herbal tea production. The present study investigated for the first time the effect of 15, 30 and 45 cm intra-row spacing (plant density) on the main constituents of sage essential oils and rosmarinic acid content. The highest content of essential oils (2.7%) and rosmarinic acid (2.0%) were obtained in plants grown using 15 cm planting space. Likewise, close spacing resulted also in a substantial content of 1,8-cineole (47-50%, GC/FID; 55-60%, GC/MS). This work indicated that 1,8-cineole chemotype was a dominant character of cultivated S. officinalis in south of Jordan. In general, the percent of α-thujone in essential oil was not affected by intra-row spacing. However, the percent of β-thujone decreased from (2-3%, GC/MS) in plants grown using 15 cm intra-row spacing to (1-2%, GC/MS) in plants grown using 30 and 45 cm intra-row spacing. The highest content of α-and β-pinene was recorded in plants grown using 45 cm planting space (8-10%, GC/FID; 5-6% GC/MS). Based on GC/MS, camphor compound was enriched (9-10%) in sage plants grown under 15 cm spacing and greater than in plants grown under 30 (6-7%) or 45 cm (5-6%) spacing. The results make the potential use of sage extracts in the treatment of some human disorders or illness an area of further research.

scholarly journals Anti-biofilm and Virulence Factor-Reducing Activities of Essential Oils and Oil Components as a Possible Option for Bacterial Infection Control

Planta Medica ◽  
2020 ◽  
Vol 86 (08) ◽  
pp. 520-537 ◽  
Author(s):  
Jürgen Reichling
Keyword(s):  
Quorum Sensing ◽  
Essential Oil ◽  
Essential Oils ◽  
Virulence Factor ◽  
Model Organisms ◽  
Regulation System ◽  
Expression Of Genes ◽  
Reporter Strains ◽  
Violacein Production ◽  
Essential Oil Compounds

AbstractPathogenic biofilm-associated bacteria that adhere to biological or nonbiological surfaces are a big challenge to the healthcare and food industries. Antibiotics or disinfectants often fail in an attempt to eliminate biofilms from those surfaces. Based on selected experimental research, this review deals with the potential biofilm-inhibiting, virulence factor-reducing, and biofilm-eradicating activities of essential oils and single essential oil compounds using Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Chromobacterium violaceum as model organisms. In addition, for the bacteria reviewed in this overview, different essential oils and essential oil compounds were reported to be able to modulate the expression of genes that are involved in the formation of autoinducer molecules, biofilms, and virulence factors. The anti-quorum sensing activity of some essential oils and single essential oil compounds was demonstrated using the gram-negative bacterium C. violaceum. Reporter strains of this bacterium produce the violet-colored compound violacein whose synthesis is regulated by quorum sensing autoinducer molecules called acylhomeserinlactones. Of great interest was the discovery that enantiomeric monoterpenes affected the quorum sensing regulation system in different ways. While the (+)-enantiomers of carvone, limonene, and borneol increased violacein formation, their (−)-analogues inhibited violacein production.For the successful eradication of biofilms and the bacteria living inside them, it is absolutely necessary that the lipophilic volatile substances can penetrate into the aqueous channels of biofilms. As shown in recent work, hydrophilic nano-delivery systems encapsulating essential oils/essential oil compounds with antibacterial effects may contribute to overcome this problem.

scholarly journals ANTIRADICAL ACTIVITY OF ESSENTIAL OILS OF FILIPENDULA ULMARIA (L.) MAXIM, HYPERICUM PERFORA-TUM L. AND PULMONARIA MOLLIS WULFEN EX HORNEM OF THE KRASNOYRSK TERRITORI FLORA

2021 ◽  
pp. 211-217
Author(s):  
Irina Dement'yevna Zykova ◽  
Aleksandr Alekseyevich Efremov
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Antiradical Activity ◽  
Component Composition ◽  
Salicylic Aldehyde ◽  
Filipendula Ulmaria ◽  
Antiradical Properties ◽  
Hypericum Perforatum L ◽  
Butyl Phthalate ◽  
Main Components

The antiradical properties of essential oils from the inflorescences of Filipendula ulmaria (L). Maxim, herbages Hypericum perforatum L. and Pulmonaria mollis Wulfen ex HORNEM., growing on the territory of the Krasnoyarsk territory were studied. For this purpose, the reaction of essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. Essential oil of the plants under study received comprehensive hydroponically. The component composition of the oils was determined by chromatography-mass spectrometry. The main components of essential oil of F. ulmaria inflorescences are methyl salicylate (28.2%), salicylic aldehyde (2.8 %) and linalool (4.9%), essential oil of H. perforatum – γ - amorphene (30.7%), δ-cadinen (7.1%), (E, E)-β-farnesene (5.5%), caryophyllene (5.0%), ledol (5.0%), essential oil of P. mollis – di-n-butyl phthalate (18.7%), docosan (13.4%), tetracosan (11.6 %). The results of the DPPH test showed that the essential oils of the inflorescences of F. ulmaria and the aboveground part of H. perforatum and P. mollis exhibit antiradical activity (ARA). According to the size of the ARA of essential oils, the studied plants can be arranged in the following row: P. mollis > F. ulmaria > H. perforatum.

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