scholarly journals Bactericidal Activity of Serum By Brucella Abortus RB51 Outer Membrane Protein’s Combined By Brucella Abortus S99 Lipopolysaccharide Induction

2021 ◽  
Author(s):  
Adeleh Attar ◽  
Hamed Afkhami ◽  
Mansoor Khaledi ◽  
Mahdi S.Sadati
Keyword(s):  
Humoral Immunity ◽  
Cellular Immunity ◽  
Bactericidal Activity ◽  
Brucella Abortus ◽  
Important Method ◽  
The Third ◽  
Serum Bactericidal Assay ◽  
Cellular And Humoral Immunity ◽  
Combined Vaccine

Abstract Brucellosis vaccines are designed to induce the cellular immunity. An effective brucellosis vaccine is one that could induce both cellular and humoral immunity. Serum Bactericidal Assay(SBA) is an important method for the determination of vaccine humoral immunity. This study is the first to observe humoral immunity in brucellosis by SBA. Extracted B.abortus LPS and OMP’s were injected to rabbits. Group1 injected by 25µg of LPS, Group2 injected by 50µg of OMP ‘s and Group3 injected by 1ml of combined vaccine, 3 times every 2 weeks. The Groups were challenged with B.abortus 544 in the second injection. Sera were separated 2 weeks after the last injection. SBA was performed, each well was streak cultured into a plate of Brucella Agar. Colony count was done for each plate. Results have shown, the third injection of the combined vaccine, had the highest titer of 1/64, and the efficacy of the vaccine was %87.71.

Assessment of cellular immunity in oil production operators with an imbalance of lipid metabolism

2020 ◽  
Vol 60 (11) ◽  
pp. 717-719
Author(s):  
Inga N. Alikina ◽  
Olga A. Kazakova
Keyword(s):  
Lipid Metabolism ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Comparison Group ◽  
Oil Production ◽  
Observation Group ◽  
Immunological Parameters ◽  
Production Factors ◽  
Cellular And Humoral Immunity ◽  
Atherosclerotic Process

Introduction. Studies indicate the high pathogenetic significance of the immune component in the development of atherosclerosis. The aim of the study is a comparative assessment of immunological parameters in workers of petrochemical production with varying degrees of imbalance in lipid metabolism and the development of the atherosclerotic process. Materials and methods. Men working at an oil production enterprise in the Perm Region were examined. The observation group consisted of oil production operators with a diagnosis of atherosclerosis, the comparison group - with dyslipidemia syndrome. To determine the parameters of lipid metabolism, the results of a biochemical blood test were used. CD-immunogram parameters were identified by flow cytometry. Specific antibodies to benzene were determined by the allergosorbent method. Results. The results of a comparative study of fat metabolism confirmed violations of the physiological ratio of lipids in the blood of oil production workers, which were expressed in a significant imbalance in the levels of lipidogram. There was an increased level of specific IgG antibodies to benzene in the observation group in relation to the comparison group. An imbalance of cellular immunity was found, which was characterized by signs of indicators activation of cellular differentiation clusters. Conclusions. Studies of immune system compartments demonstrate excessive activation of cellular and humoral immunity in oil production workers under the influence of a combination of harmful production factors. The simultaneously formed imbalance of lipid metabolism is associated with various degrees of clinical manifestation of atherosclerotic disorders, with the influence of harmful production factors, aggressiveness of cellular and humoral immunity, and smoking.

scholarly journals Efficiency of human plague vaccination in tuvinian natural plague focus. Message 2: dynamics of immune status indicators after revaccination

2021 ◽  
Vol 6 (5) ◽  
pp. 44-56
Author(s):  
K. M. Korytov ◽  
V. I. Dubrovina ◽  
V. V. Voytkova ◽  
A. B. Pyatidesyatnikova ◽  
E. A. Glushkov ◽  
...  
Keyword(s):  
Humoral Immunity ◽  
Lymphocyte Subpopulation ◽  
Epidemiological Surveillance ◽  
Immunological Monitoring ◽  
Tnf Α ◽  
The Republic ◽  
Cellular And Humoral Immunity ◽  
Immunological Protection ◽  
Plague Vaccine

Relevance. In Russia, the live plague vaccine (LPV) is used for specific prophylaxis of plague. Immunological monitoring of humans vaccinated by LPV in order to search for informative diagnostic markers, as well as to improve the tactics of epidemiological surveillance of plague enzootic territories is an urgent area of research.The aim is to assess the parameters of cellular and humoral immunity in humans revaccinated by LPV who permanently reside on territory of the Tuvinian natural plague focus.Materials and methods. The study involved 76 volunteers from the Republic of Tuva, revaccinated by LPV. Blood sampling was performed before vaccination and 1, 3, and 6 months after revaccination. The study included the determination of cytokine production (IFN-γ, IL-4, TNF-α), specific antibodies, immunoglobulins (IgM, IgG, IgA and IgE) and lymphocyte subpopulation composition (CD3, CD4, CD8, CD16, CD19, immunoregulatory index).Results. A decrease in IgG and IgM and an increase in IgA were found after vaccination with LPV and their increase after revaccination. Correlation relationships were revealed between immunoglobulins, B cells and IL-4. Revaccination leads to an increase seroconversion. The activation of humoral immunity in humans vaccinated against plague is also evidenced by dynamics of changes in the subpopulation composition: an increase in B-lymphocytes and natural killer cells, a decrease in T-helpers and immunoregulatory index, and cellular immunity stimulation is an increase in spontaneous and induced production of pro- and anti-inflammatory cytokines.Conclusion. It has been shown that LPV is capable of causing the body’s immune restructuring and activating the cellular and humoral mechanisms of immunological protection. For a complete understanding of the development and preservation of  antiplague immunity, it is necessary to continue the annual immunological monitoring of the population living on the territory of the Tuvinian natural plague focus, using additional modern research methods. 

scholarly journals PSVI-22 Immunity indicators of calves obtained from cows with ketosis during the treatment process

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 199-199
Author(s):  
Sergey Kovalev ◽  
Anastasia Nikitina ◽  
Polina Anipchenko ◽  
Anatoly Stekolnikov ◽  
Pavel Kiselenko ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Treatment Process ◽  
Absolute Number ◽  
Relative Content ◽  
Newborn Calves ◽  
Cellular And Humoral Immunity

Abstract The purpose of these studies was to determine the indicators of cellular and humoral immunity of calves. Compared to calves obtained from untreated cows, the number of T-lymphocytes in young calves born from cows treated with Timolin increased the level of T-cells to 32.9%, while using Timosplenin 45%. As a result of the use of immunomodulators in treating cows with ketosis in mothers, the relative content of T-lymphocytes in the blood of calves born from them increased when using the drug Leucogen by 1.7 times, Timosplenin by 2.3 times, Timalin in 2.2 times. The use of Leucogen for cows with ketosis contributed to an increase in the percentage of B cells in the blood of calves born by 23%, Timosplenin 83%, and Timalin 42%. At the same time, the absolute number of B-lymphocytes in the treatment of “Leucogen” increased 1.9 times and reached the level of healthy calves, in the treatment with the preparations “Timosplenin” and “Timalin” - the increase was, respectively, 3.0 and 2.3 times and was higher this indicator in young animals born from healthy calves. Thus, the inclusion of dry immunomodulators in the treatment regimen of patients with ketosis leads not only to normalization of metabolism, but is also accompanied by an increase in the level of cellular immunity factors in calves born from them. Under the influence of “Leucogen,” the percentage of lymphocytes was normalized, whereas with the introduction of “Timalin” and “Timosplenin,” the content of this type of cells in newborn calves even exceeded those in healthy young.

scholarly journals Humoral and Cellular Immunity in Children with Mycoplasma pneumoniae Infection: a 1-Year Prospective Study

2005 ◽  
Vol 12 (10) ◽  
pp. 1246-1250 ◽  
Author(s):  
Iwona Stelmach ◽  
Malgorzata Podsiadłowicz-Borzęcka ◽  
Tomasz Grzelewski ◽  
Pawel Majak ◽  
Wlodzimierz Stelmach ◽  
...  
Keyword(s):  
Prospective Study ◽  
Mycoplasma Pneumoniae ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Immunoglobulin G ◽  
Serum Immunoglobulin ◽  
Humoral And Cellular Immunity ◽  
Cellular And Humoral Immunity ◽  
Lymphocyte Phenotypes ◽  
Mycoplasma Pneumoniae Infection

ABSTRACT To determine whether children have persistent abnormalities in cellular and humoral immunity development after acute Mycoplasma pneumoniae infection, serum immunoglobulin G (IgG), IgA, IgM, and IgE levels and lymphocyte phenotypes were determined. There were no changes in the levels of IgG, IgM, IgA, or CD4+ or CD19+ lymphocytes that were measured in M. pneumoniae-positive patients after 3 months or after 12 months, but there were increases in these in M. pneumoniae-negative patients. Serum IgE increased in M. pneumoniae-positive patients. We have shown alterations in immunity development after M. pneumoniae infection.

scholarly journals Changes of the Immune Status in Patients with Purulent Peritonitis

2016 ◽  
Vol 23 (3) ◽  
Author(s):  
Serhiy I Savolyuk ◽  
Maksim A Gudz ◽  
Andriy A Zhmur
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Humoral Immunity ◽  
Diagnostic Value ◽  
Purulent Peritonitis ◽  
Stepwise Reduction ◽  
Cellular And Humoral Immunity ◽  
End Stage ◽  
The Impact

The analysis of cellular and humoral immunity indicators in the blood of patients with purulent peritonitis (PP) with the determination of their diagnostic value while determining the stage of peritonitis was performed. The levels of CD3, CD4, CD8, CD4 / CD8, CD19, CD16, CD95, CD25, IgA, IgM, IgG during hospitalization of 126 patients with PP was investigated. Monitoring of immune responsiveness stated regular changes in cellular and humoral immunity caused by the influence of cytokine profile, describing immunosuppression, the extent of which depends on the length and stage of PP. The progressive deepening of PP stages (in reactive and toxic stage) led to an increase in white blood cell count, CD95, due to the influence of dose-dependent increase in IL-6, CD19, IgM, G, and NK-cells (CD16) and CD25, the dynamics of which is closely related and dependent of IL-2.This was accompanied by a progressive lymphocytopenia, a decrease in CD3, CD4, which at constant or slightly increasing concentration led to decrease in CD8 IRI (CD4 / CD8) caused by the impact of immunosuppressive IL-6, stepwise reduction of IgA. Severe secondary immunodeficiency was observed in patients with end-stage PP and lymphocytopenia progressed along with the growth of white blood cell and marker CD95 with disruption of T-lymphocytes subsets (CD4 decrease at a constant or insignificant growth of CD8) with critical IRI indicators. The decrease in CD25, CD16 and CD19 dysfunction with sharp inhibition of the antibodies synthesis – decrease in IgM and G, A concentrations was also determined.

scholarly journals Cellular and humoral immunity after the third dose of SARS-CoV-2 vaccine in patients treated with rituximab

2021 ◽  
Author(s):  
Renaud Felten ◽  
Floriane Gallais ◽  
Cédric Schleiss ◽  
Emmanuel Chatelus ◽  
Rose-Marie Javier ◽  
...  
Keyword(s):  
Humoral Immunity ◽  
The Third ◽  
Cellular And Humoral Immunity

Investigations on skeletal growth zones via bone scans as base of determination of optimal time for surgery in mandibular asymmetry

2000 ◽  
Vol 39 (05) ◽  
pp. 121-126 ◽  
Author(s):  
R. Werz ◽  
P. Reuland
Keyword(s):  
Bone Growth ◽  
Region Of Interest ◽  
Tracer Uptake ◽  
Optimal Time ◽  
Mandibular Bone ◽  
The Third ◽  
Mandibular Asymmetry ◽  
Bone Scans ◽  
Epiphyseal Plates

Summary Aim of the study was to find out wether there is a common stop of growth of mandibular bone, so that no individual determination of the optimal time for surgery in patients with asymmetric mandibular bone growth is needed. As there are no epiphyseal plates in the mandibular bone, stop of growth cannot be determined on X-ray films. Methods: Bone scans of 731 patients [687 patients (324 male, 363 female) under 39 y for exact determination of end of growth and 44 (21 male, 23 female) patients over 40 y for evaluation of nongrowth dependant differences in tracer uptake] were reviewed for the study. All the patients were examined 3 hours after injection of 99mTc-DPD. Tracer uptake was measured by region of interest technique in different points of the mandibular bone and in several epiphyseal plates of extremities. Results: Tracer uptake in different epiphyseal plates of the extremities shows strong variation with age and good correlation with reported data of bone growth and closure of the epiphyseal plates. The relative maximum of bone activity is smaller in mandibular bone than in epiphyseal plates, which show well defined peaks, ending at 15-18 years in females and at 18-21 years in males. In contrast, mandibular bone shows no well defined end of growing but a gradually reduction of bone activity which remains higher than bone activity in epiphyseal plates over several years. Conclusion: No well defined end of growth of mandibular bone exists. The optimal age for surgery of asymmetric mandibular bone growth is not before the middle of the third decade of life, bone scans performed earlier for determination of bone growth can be omitted. Bone scans performed at the middle of the third decade of life help to optimize the time of surgical intervention.

THE FACTORS OF CAREER SELF-DETERMINATION OF STUDENTS OF HIGHER EDUCATION INSTITUTIONS

2019 ◽  
Vol 7 (1) ◽  
pp. 9-20
Author(s):  
Inna Yeung
Keyword(s):  
Higher Education ◽  
Higher Education Institutions ◽  
Well Being ◽  
Young Person ◽  
Self Determination ◽  
Full Time ◽  
Actual Problem ◽  
The Third ◽  
The Right

Choice of profession is a social phenomenon that every person has to face in life. Numerous studies convince us that not only the well-being of a person depends on the chosen work, but also his attitude to himself and life in general, therefore, the right and timely professional choice is very important. Research about factors of career self-determination of students of higher education institutions in Ukraine shows that self-determination is an important factor in the socialization of young person, and the factors that determine students' career choices become an actual problem of nowadays. The present study involved full-time and part-time students of Institute of Philology and Mass Communications of Open International University of Human Development "Ukraine" in order to examine the factors of career self-determination of students of higher education institutions (N=189). Diagnostic factors of career self-determination of students studying in the third and fourth year were carried out using the author's questionnaire. Processing of obtained data was carried out using the Excel 2010 program; factorial and comparative analysis were applied. Results of the study showed that initial stage of career self-determination falls down on the third and fourth studying year at the university, when an image of future career and career orientations begin to form. At the same time, the content of career self-determination in this period is contradictory and uncertain, therefore, the implementation of pedagogical support of this process among students is effective.

Comparative assessment of informational content of the equations of F.P. Hadlock and the computer program of V.N. Demidov in determination of term of gestation and fetal weight in the III trimester of gestation

2017 ◽  
Author(s):  
E.A. Derkach , O.I. Guseva
Keyword(s):  
Computer Program ◽  
High Precision ◽  
Gestational Age ◽  
Computer Programs ◽  
Comparative Assessment ◽  
Fetal Weight ◽  
Informational Content ◽  
Third Trimester ◽  
The Third

Objectives: to compare the accuracy of equations F.P. Hadlock and computer programs by V.N. Demidov in determining gestational age and fetal weight in the third trimester of gestation. Materials: 328 patients in terms 36–42 weeks of gestation are examined. Ultrasonography was performed in 0–5 days prior to childbirth. Results: it is established that the average mistake in determination of term of pregnancy when using the equation of F.P. Hadlock made 12,5 days, the computer program of V.N. Demidov – 4,4 days (distinction 2,8 times). The mistake within 4 days, when using the equation of F.P. Hadlock has met on average in 23,1 % of observations, the computer program of V.N. Demidov — 65,9 % (difference in 2,9 times). The mistake more than 10 days, took place respectively in 51,7 and 8,2 % (distinction by 6,3 times). At a comparative assessment of size of a mistake in determination of fetal mass it is established that when using the equation of F.P. Hadlock it has averaged 281,0 g, at application of the computer program of V.N. Demidov — 182,5 g (distinction of 54 %). The small mistake in the mass of a fetus which isn't exceeding 200 g at application of the equation of F.P. Hadlock has met in 48,1 % of cases and the computer program of V.N. Demidov — 64,0 % (distinction of 33,1 %). The mistake exceeding 500 g has been stated in 18 % (F.P. Hadlock) and 4,3 % (V.N. Demidov) respectively (distinction 4,2 times). Conclusions: the computer program of V.N. Demidov has high precision in determination of term of a gestation and mass of a fetus in the III pregnancy.

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