scholarly journals Humoral and Cellular Immunity in Children with Mycoplasma pneumoniae Infection: a 1-Year Prospective Study

2005 ◽  
Vol 12 (10) ◽  
pp. 1246-1250 ◽  
Author(s):  
Iwona Stelmach ◽  
Malgorzata Podsiadłowicz-Borzęcka ◽  
Tomasz Grzelewski ◽  
Pawel Majak ◽  
Wlodzimierz Stelmach ◽  
...  
Keyword(s):  
Prospective Study ◽  
Mycoplasma Pneumoniae ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Immunoglobulin G ◽  
Serum Immunoglobulin ◽  
Humoral And Cellular Immunity ◽  
Cellular And Humoral Immunity ◽  
Lymphocyte Phenotypes ◽  
Mycoplasma Pneumoniae Infection

ABSTRACT To determine whether children have persistent abnormalities in cellular and humoral immunity development after acute Mycoplasma pneumoniae infection, serum immunoglobulin G (IgG), IgA, IgM, and IgE levels and lymphocyte phenotypes were determined. There were no changes in the levels of IgG, IgM, IgA, or CD4+ or CD19+ lymphocytes that were measured in M. pneumoniae-positive patients after 3 months or after 12 months, but there were increases in these in M. pneumoniae-negative patients. Serum IgE increased in M. pneumoniae-positive patients. We have shown alterations in immunity development after M. pneumoniae infection.

Assessment of cellular immunity in oil production operators with an imbalance of lipid metabolism

2020 ◽  
Vol 60 (11) ◽  
pp. 717-719
Author(s):  
Inga N. Alikina ◽  
Olga A. Kazakova
Keyword(s):  
Lipid Metabolism ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Comparison Group ◽  
Oil Production ◽  
Observation Group ◽  
Immunological Parameters ◽  
Production Factors ◽  
Cellular And Humoral Immunity ◽  
Atherosclerotic Process

Introduction. Studies indicate the high pathogenetic significance of the immune component in the development of atherosclerosis. The aim of the study is a comparative assessment of immunological parameters in workers of petrochemical production with varying degrees of imbalance in lipid metabolism and the development of the atherosclerotic process. Materials and methods. Men working at an oil production enterprise in the Perm Region were examined. The observation group consisted of oil production operators with a diagnosis of atherosclerosis, the comparison group - with dyslipidemia syndrome. To determine the parameters of lipid metabolism, the results of a biochemical blood test were used. CD-immunogram parameters were identified by flow cytometry. Specific antibodies to benzene were determined by the allergosorbent method. Results. The results of a comparative study of fat metabolism confirmed violations of the physiological ratio of lipids in the blood of oil production workers, which were expressed in a significant imbalance in the levels of lipidogram. There was an increased level of specific IgG antibodies to benzene in the observation group in relation to the comparison group. An imbalance of cellular immunity was found, which was characterized by signs of indicators activation of cellular differentiation clusters. Conclusions. Studies of immune system compartments demonstrate excessive activation of cellular and humoral immunity in oil production workers under the influence of a combination of harmful production factors. The simultaneously formed imbalance of lipid metabolism is associated with various degrees of clinical manifestation of atherosclerotic disorders, with the influence of harmful production factors, aggressiveness of cellular and humoral immunity, and smoking.

Host Immune Responses Against CT Antigens in Multiple Myeloma Patients.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3492-3492 ◽  
Author(s):  
Nikoletta Lendvai ◽  
Sacha Gnjatic ◽  
Erika Ritter ◽  
Yao-Tseng Chen ◽  
Christina Coughlin ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Immune Responses ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Therapeutic Vaccine ◽  
Type I ◽  
Humoral And Cellular Immunity ◽  
Ct Antigen ◽  
Ct Antigens

Abstract The type I Melanoma Antigen GEne (MAGE) proteins belong to the Cancer-Testis (CT) family of tumor-associated antigens and are widely expressed in solid and hematologic malignancies. They are immunogenic and frequently elicit spontaneous immune responses in patients with CT antigen-expressing tumors, particularly in malignant melanoma. In melanoma patients, there is high concordance between humoral and cellular immunity. Based on these findings, CT antigens are widely investigated as potential antigenic targets for tumor-specific therapeutic vaccines. We previously showed that the type I MAGE proteins CT7 (MAGE-C1), CT10 (MAGE-C2) and MAGE-A3 were commonly detected in primary myeloma specimens, and expression of CT7 and MAGE-A3 was correlated with abnormally elevated plasma cell proliferation. These findings suggest that type I MAGE may be rational targets for vaccine therapy in multiple myeloma. Therefore, it is important to determine if type I MAGE elicit cellular or humoral immune responses in myeloma patients. To investigate this hypothesis, we assessed cellular immunity against CT7 and humoral immunity against a broad panel of CT antigens. To quantify CT7-specific cellular immunity, expanded, polyclonal pools of T cells from the bone marrow, the tumor microenvironment, were co-cultured in interferon gamma (IFNγ) ELISpot assays with autologous antigen-presenting cells (APC) transduced with in vitro transcribed mRNA coding for CT7 or control antigens. CT antigen-specific humoral immunity was examined by ELISA assay using patient serum or plasma and recombinant CT antigens. This analysis demonstrated that 2/9 patients exhibited specific T cell immunity against CT7 in their bone marrow lymphocytes as measured by IFNγ secretion. These same two patients had positive titers for other CT antigens; one for MAGE-A1 (another type I MAGE), the other for SSX-1 (a structurally distinct CT antigen). Interestingly, neither patient had positive serology for CT7. Serum from 16 other myeloma patients did not have detectible antibody titers for a broad panel of CT antigens. These results show that CT antigens are immunogenic in myeloma patients, with cellular responses against CT7 and humoral responses against MAGE-A1 and SSX-1. However, unlike other types of cancer, there appears to be discordance between humoral and cellular immunity against CT7 in multiple myeloma. This may be due in part to the significant derangements of humoral immunity in this disease. These results support further investigation of immunologic therapies targeting type I MAGE in myeloma, especially therapeutic vaccine strategies.

scholarly journals Impact of Preexisting Vector-Specific Immunity on Vaccine Potency: Characterization of Listeria monocytogenes-Specific Humoral and Cellular Immunity in Humans and Modeling Studies Using Recombinant Vaccines in Mice

2009 ◽  
Vol 77 (9) ◽  
pp. 3958-3968 ◽  
Author(s):  
Meredith L. Leong ◽  
Johannes Hampl ◽  
Weiqun Liu ◽  
Shruti Mathur ◽  
Keith S. Bahjat ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
High Titer ◽  
Listeriolysin O ◽  
Wild Type ◽  
Vaccine Platform ◽  
Humoral And Cellular Immunity ◽  
Specific Immunity ◽  
Modeling Studies

ABSTRACT Recombinant live-attenuated Listeria monocytogenes is currently being developed as a vaccine platform for treatment or prevention of malignant and infectious diseases. The effectiveness of complex biologic vaccines, such as recombinant viral and bacterial vectors, can be limited by either preexisting or vaccine-induced vector-specific immunity. We characterized the level of L. monocytogenes-specific cellular and humoral immunity present in more than 70 healthy adult subjects as a first step to understanding its possible impact on the efficacy of L. monocytogenes-based vaccines being evaluated in early-phase clinical trials. Significant L. monocytogenes-specific humoral immunity was not measured in humans, consistent with a lack of antibodies in mice immunized with wild-type L. monocytogenes. Cellular immune responses specific for listeriolysin O, a secreted bacterial protein required for potency of L. monocytogenes-derived vaccines, were detected in approximately 60% of human donors tested. In mice, while wild-type L. monocytogenes did not induce significant humoral immunity, attenuated L. monocytogenes vaccine strains induced high-titer L. monocytogenes-specific antibodies when given at high doses used for immunization. Passive transfer of L. monocytogenes-specific antiserum to naïve mice had no impact on priming antigen-specific immunity in mice immunized with a recombinant L. monocytogenes vaccine. In mice with preexisting L. monocytogenes-specific immunity, priming of naïve T cells was not prevented, and antigen-specific responses could be boosted by additional vaccinations. For the first time, our findings establish the level of L. monocytogenes-specific cellular immunity in healthy adults, and, together with modeling studies performed with mice, they support the scientific rationale for repeated L. monocytogenes vaccine immunization regimens to elicit a desired therapeutic effect.

scholarly journals PSVI-22 Immunity indicators of calves obtained from cows with ketosis during the treatment process

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 199-199
Author(s):  
Sergey Kovalev ◽  
Anastasia Nikitina ◽  
Polina Anipchenko ◽  
Anatoly Stekolnikov ◽  
Pavel Kiselenko ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Treatment Process ◽  
Absolute Number ◽  
Relative Content ◽  
Newborn Calves ◽  
Cellular And Humoral Immunity

Abstract The purpose of these studies was to determine the indicators of cellular and humoral immunity of calves. Compared to calves obtained from untreated cows, the number of T-lymphocytes in young calves born from cows treated with Timolin increased the level of T-cells to 32.9%, while using Timosplenin 45%. As a result of the use of immunomodulators in treating cows with ketosis in mothers, the relative content of T-lymphocytes in the blood of calves born from them increased when using the drug Leucogen by 1.7 times, Timosplenin by 2.3 times, Timalin in 2.2 times. The use of Leucogen for cows with ketosis contributed to an increase in the percentage of B cells in the blood of calves born by 23%, Timosplenin 83%, and Timalin 42%. At the same time, the absolute number of B-lymphocytes in the treatment of “Leucogen” increased 1.9 times and reached the level of healthy calves, in the treatment with the preparations “Timosplenin” and “Timalin” - the increase was, respectively, 3.0 and 2.3 times and was higher this indicator in young animals born from healthy calves. Thus, the inclusion of dry immunomodulators in the treatment regimen of patients with ketosis leads not only to normalization of metabolism, but is also accompanied by an increase in the level of cellular immunity factors in calves born from them. Under the influence of “Leucogen,” the percentage of lymphocytes was normalized, whereas with the introduction of “Timalin” and “Timosplenin,” the content of this type of cells in newborn calves even exceeded those in healthy young.

scholarly journals Magnetic-lazer modulation of specific humoral immunity in pregnant women with anemia

2011 ◽  
Vol 10 (3) ◽  
pp. 149-154
Author(s):  
S. Yu. Yuriyev ◽  
I. V. Aristova ◽  
Ye. F. Levitsky ◽  
S. S. Shakhova ◽  
S. A. Krotov
Keyword(s):  
Helicobacter Pylori ◽  
Comparative Analysis ◽  
Gastrointestinal Tract ◽  
Pregnant Women ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Humoral And Cellular Immunity ◽  
Mild Anemia ◽  
Laser Effect ◽  
Stimulation Of

This article presents the comparative analysis of humoral and cellular immunity indicators under the influence of magneticlaser effect in 2 frequency ranges (150—1 5000 Hz) for treatment of pregnant women with mild anemia against the background of chronic gastritis, associated with contamination gastrointestinal tract Helicobacter pylori. The application of magnetic-laser effect in frequency 150 Hz allows to raise of hemoglobin lowers the titre of antibodies to Helicobacter pylori. The moderate stimulation of Th1 link withoit activation of humoral immunity is optimal in treatment of benign anemia of pregnancy.

scholarly journals Bactericidal Activity of Serum By Brucella Abortus RB51 Outer Membrane Protein’s Combined By Brucella Abortus S99 Lipopolysaccharide Induction

2021 ◽  
Author(s):  
Adeleh Attar ◽  
Hamed Afkhami ◽  
Mansoor Khaledi ◽  
Mahdi S.Sadati
Keyword(s):  
Humoral Immunity ◽  
Cellular Immunity ◽  
Bactericidal Activity ◽  
Brucella Abortus ◽  
Important Method ◽  
The Third ◽  
Serum Bactericidal Assay ◽  
Cellular And Humoral Immunity ◽  
Combined Vaccine

Abstract Brucellosis vaccines are designed to induce the cellular immunity. An effective brucellosis vaccine is one that could induce both cellular and humoral immunity. Serum Bactericidal Assay(SBA) is an important method for the determination of vaccine humoral immunity. This study is the first to observe humoral immunity in brucellosis by SBA. Extracted B.abortus LPS and OMP’s were injected to rabbits. Group1 injected by 25µg of LPS, Group2 injected by 50µg of OMP ‘s and Group3 injected by 1ml of combined vaccine, 3 times every 2 weeks. The Groups were challenged with B.abortus 544 in the second injection. Sera were separated 2 weeks after the last injection. SBA was performed, each well was streak cultured into a plate of Brucella Agar. Colony count was done for each plate. Results have shown, the third injection of the combined vaccine, had the highest titer of 1/64, and the efficacy of the vaccine was %87.71.

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