scholarly journals Comparative Transcriptomics Reveals Osmotic and Ionic Stress Key Genes Contributing To Salinity Tolerance Differential in Two Pak Choi Cultivars

2021 ◽  
Author(s):  
Xueling Du ◽  
Rugang Yu ◽  
Changqian Shi ◽  
Ying Wang ◽  
Rui Meng ◽  
...  
Keyword(s):  
Salinity Tolerance ◽  
De Novo ◽  
Nacl Stress ◽  
Pak Choi ◽  
Kegg Pathway Analysis ◽  
Digital Expression ◽  
Ionic Stress ◽  
Osmotic And Ionic Stress ◽  
The Difference ◽  
Stress Related Genes

Abstract Background: Pak choi is an important leafy vegetable crop. Salinity is among the most harmful agents that negatively influence pak choi yield. However, the mechanism of salinity tolerance in pak choi has not been well understood. In this study, the root transcriptomics of two cultivars differing in salinity tolerant, Shanghaijimaocai (S, salinity tolerant) and Te’aiqing (T, salinity sensitive), were investigated under 0 and 100 mM NaCl treatments.Results: Using de novo assembly, 214,952 assembled unigenes were generated. Totals of 6765, 2454, 2451 and 5798 differentially expressed unigenes (DEUs) were identified in comparison of S100/S0, T100/T0, S0/T0 and S100/T100, respectively. Shanghaijimaocai is more sensitive to NaCl stress than Te’aiqing in terms of root transcriptomics. Based on GO and KEGG pathway analysis, several osmotic and ionic stress-related genes including MP3K18, PYL8, PP2C15/16/49, ARF2, bHLH112, bZIP43, COL5, CDF1/3, ERF25/60, HSFA6, MYBS3/59/92/CCA1/PHL5, POD21, GOLS7, CIPK4/7/12, NHX7, SLAH1 and ALMT13, showed higher expression in Shanghaijimaocai than in Te’aiqing. These genes, therefore, might be contributed to the difference in salinity tolerance. Moreover, the physiological shift of peroxidase activity was in accordance with dynamic transcript profile of the relevant unigenes. Conclusions: We determined digital expression profile and discovered a broad survey of unigenes associated with the difference of salinity tolerance between Shanghaijimaocai and Te’aiqing. These findings would be useful for further functional analysis as potential targets to improve resistance to salinity stress via genetic engineering.

Prognostic implication of CT-FFR based functional SYNTAX score in patients with de novo three-vessel disease

2020 ◽  
Author(s):  
Hong Yan Qiao ◽  
Jian Hua Li ◽  
U Joseph Schoepf ◽  
Richard R Bayer ◽  
Fiona C Tinnefeld ◽  
...  
Keyword(s):  
De Novo ◽  
Predictive Accuracy ◽  
Computed Tomographic Angiography ◽  
Syntax Score ◽  
Cardiac Events ◽  
Fractional Flow ◽  
Computed Tomographic ◽  
Flow Reserve ◽  
The Difference ◽  
Functional Syntax

Abstract Aims This study was aimed at investigating whether a machine learning (ML)-based coronary computed tomographic angiography (CCTA) derived fractional flow reserve (CT-FFR) SYNTAX score (SS), ‘Functional SYNTAX score’ (FSSCTA), would predict clinical outcome in patients with three-vessel coronary artery disease (CAD). Methods and results The SS based on CCTA (SSCTA) and ICA (SSICA) were retrospectively collected in 227 consecutive patients with three-vessel CAD. FSSCTA was calculated by combining the anatomical data with functional data derived from a ML-based CT-FFR assessment. The ability of each score system to predict major adverse cardiac events (MACE) was compared. The difference between revascularization strategies directed by the anatomical SS and FSSCTA was also assessed. Two hundred and twenty-seven patients were divided into two groups according to the SSCTA cut-off value of 22. After determining FSSCTA for each patient, 22.9% of patients (52/227) were reclassified to a low-risk group (FSSCTA ≤ 22). In the low- vs. intermediate-to-high (>22) FSSCTA group, MACE occurred in 3.2% (4/125) vs. 34.3% (35/102), respectively (P < 0.001). The independent predictors of MACE were FSSCTA (OR = 1.21, P = 0.001) and diabetes (OR = 2.35, P = 0.048). FSSCTA demonstrated a better predictive accuracy for MACE compared with SSCTA (AUC: 0.81 vs. 0.75, P = 0.01) and SSICA (0.81 vs. 0.75, P < 0.001). After FSSCTA was revealed, 52 patients initially referred for CABG based on SSCTA would have been changed to PCI. Conclusion Recalculating SS by incorporating lesion-specific ischaemia as determined by ML-based CT-FFR is a better predictor of MACE in patients with three-vessel CAD. Additionally, the use of FSSCTA may alter selected revascularization strategies in these patients.

scholarly journals Malpigmentation of Common Sole (Solea solea) during Metamorphosis Is Associated with Differential Synaptic-Related Gene Expression

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2273
Author(s):  
Menelaos Kavouras ◽  
Emmanouil E. Malandrakis ◽  
Ewout Blom ◽  
Kyriaki Tsilika ◽  
Theodoros Danis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nervous System ◽  
De Novo ◽  
Close Association ◽  
Ionic Channels ◽  
Long Term Potentiation ◽  
General Term ◽  
The Difference ◽  
Common Sole

In farmed flatfish, such as common sole, color disturbances are common. Dyschromia is a general term that includes the color defects on the blind and ocular sides of the fish. The purpose was to examine the difference in gene expression between normal pigmented and juveniles who present ambicoloration. The analysis was carried out with next-generation sequencing techniques and de novo assembly of the transcriptome. Transcripts that showed significant differences (FDR < 0.05) in the expression between the two groups, were related to those of zebrafish (Danio rerio), functionally identified, and classified into categories of the gene ontology. The results revealed that ambicolorated juveniles exhibit a divergent function, mainly of the central nervous system at the synaptic level, as well as the ionic channels. The close association of chromophore cells with the growth of nerve cells and the nervous system was recorded. The pathway, glutamate binding–activation of AMPA and NMDA receptors–long-term stimulation of postsynaptic potential–LTP (long term potentiation)–plasticity of synapses, appears to be affected. In addition, the development of synapses also seems to be affected by the interaction of the LGI (leucine-rich glioma inactivated) protein family with the ADAM (a disintegrin and metalloprotease) ones.

Supine Traction Versus Prone Bending Radiographs for Assessing the Curve Flexibility in Spinal Deformity

2021 ◽  
pp. 219256822097913
Author(s):  
Niek te Hennepe ◽  
Maarten Spruit ◽  
Martin H. Pouw ◽  
Marco Hinderks ◽  
Petra Heesterbeek
Keyword(s):  
Idiopathic Scoliosis ◽  
Spinal Deformity ◽  
Retrospective Cohort ◽  
De Novo ◽  
Traction Force ◽  
Degenerative Lumbar Scoliosis ◽  
Curve Correction ◽  
Subgroup Analyses ◽  
The Difference ◽  
Side Bending

Study Design: Retrospective cohort study. Objectives: No consensus exists among surgeons on which radiologic method to prefer for the assessment of curve flexibility in spinal deformity. The objective of this study was to evaluate the difference in curve correction on supine traction radiographs versus prone side bending radiographs. Methods: A retrospective analysis of idiopathic scoliosis (IS), degenerative idiopathic scoliosis (DIS) and de novo degenerative lumbar scoliosis (DNDLS) patients was performed on supine traction as well as prone bending films (when available). Age, weight, traction force, diagnosis and Cobb angles of the primary and secondary curves were extracted. Differences in curve correction (percentages) on traction versus prone bending radiographs were analyzed for the primary and secondary curve. Subgroup analyses were performed for the 3 different diagnoses. Results: In total, 170 patients were eligible for inclusion. 43 were diagnosed with IS, 58 with DIS and 69 with DNDLS. For the primary curve, greater curve correction was obtained with bending in the DNDLS group ( P < 0.001). In the DIS group, there was a trend toward more correction on bending ( P = 0.054). In de IS group no difference was found. For the secondary curve, bending showed more curve correction in the IS and DIS group ( P = 0.002 and P <0.001). No difference was found in the DNDLS group. Conclusion: Compared to traction radiographs, bending radiographs better serve the purpose of curve flexibility assessment of IS, DIS and DNDLS spinal deformity, despite the fact that patients are exposed to more radiation.

scholarly journals Effects of isoenergetic overfeeding of either carbohydrate or fat in young men

2000 ◽  
Vol 84 (2) ◽  
pp. 233-245 ◽  
Author(s):  
Ole Lammert ◽  
Niels Grunnet ◽  
Peter Faber ◽  
Kirsten Schroll Bjørnsbo ◽  
John Dich ◽  
...  
Keyword(s):  
Fat Mass ◽  
De Novo ◽  
Fat Free Mass ◽  
Whole Body ◽  
Distribution Analysis ◽  
Mass Isotopomer Distribution Analysis ◽  
The Difference ◽  
Normal Men ◽  
Plasma Leptin ◽  
Mass Increase

Ten pairs of normal men were overfed by 5 MJ/d for 21 d with either a carbohydrate-rich or a fat-rich diet (C- and F-group). The two subjects in each pair were requested to follow each other throughout the day to ensure similar physical activity and were otherwise allowed to maintain normal daily life. The increase in body weight, fat free mass and fat mass showed great variation, the mean increases being 1·5 kg, 0·6 kg and 0·9 kg respectively. No significant differences between the C- and F-group were observed. Heat production during sleep did not change during overfeeding. The RQ during sleep was 0·86 and 0·78 in the C- and F-group respectively. The accumulated faecal loss of energy, DM, carbohydrate and protein was significantly higher in the C- compared with the F-group (30, 44, 69 and 51 % higher respectively), whereas the fat loss was the same in the two groups. N balance was not different between the C- and F-group and was positive. Fractional contribution from hepatic de novo lipogenesis, as measured by mass isotopomer distribution analysis after administration of [1-13C]acetate, was 0·20 and 0·03 in the C-group and the F-group respectively. Absolute hepatic de novo lipogenesis in the C-group was on average 211 g per 21 d. Whole-body de novo lipogenesis, as obtained by the difference between fat mass increase and dietary fat available for storage, was positive in six of the ten subjects in the C-group (mean 332 (SEM 191) g per 21 d). The change in plasma leptin concentration was positively correlated with the change in fat mass. Thus, fat storage during overfeeding of isoenergetic amounts of diets rich in carbohydrate or in fat was not significantly different, and carbohydrates seemed to be converted to fat by both hepatic and extrahepatic lipogenesis.

Similarities and differences between the responses to osmotic and ionic stress in quinoa from a water use perspective

2018 ◽  
Vol 203 ◽  
pp. 344-352 ◽  
Author(s):  
Jon Miranda-Apodaca ◽  
Ander Yoldi-Achalandabaso ◽  
Aitor Aguirresarobe ◽  
Arantza del Canto ◽  
Usue Pérez-López
Keyword(s):  
Water Use ◽  
Ionic Stress ◽  
Similarities And Differences ◽  
Osmotic And Ionic Stress

scholarly journals Physiological and Biochemical Responses to Salt Stress of Alfalfa Populations Selected for Salinity Tolerance and Grown in Symbiosis with Salt-Tolerant Rhizobium

Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 569
Author(s):  
Annick Bertrand ◽  
Craig Gatzke ◽  
Marie Bipfubusa ◽  
Vicky Lévesque ◽  
Francois P. Chalifour ◽  
...  
Keyword(s):  
Amino Acids ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Water Content ◽  
Salinity Tolerance ◽  
Osmotic Adjustment ◽  
Recurrent Selection ◽  
Aromatic Amino Acids ◽  
Nacl Stress ◽  
Salt Tolerant

Alfalfa and its rhizobial symbiont are sensitive to salinity. We compared the physiological responses of alfalfa populations inoculated with a salt-tolerant rhizobium strain, exposed to five NaCl concentrations (0, 20, 40, 80, or 160 mM NaCl). Two initial cultivars, Halo (H-TS0) and Bridgeview (B-TS0), and two populations obtained after three cycles of recurrent selection for salt tolerance (H-TS3 and B-TS3) were compared. Biomass, relative water content, carbohydrates, and amino acids concentrations in leaves and nodules were measured. The higher yield of TS3-populations than initial cultivars under salt stress showed the effectiveness of our selection method to improve salinity tolerance. Higher relative root water content in TS3 populations suggests that root osmotic adjustment is one of the mechanisms of salt tolerance. Higher concentrations of sucrose, pinitol, and amino acid in leaves and nodules under salt stress contributed to the osmotic adjustment in alfalfa. Cultivars differed in their response to recurrent selection: under a 160 mM NaCl-stress, aromatic amino acids and branched-chain amino acids (BCAAs) increased in nodules of B-ST3 as compared with B-TS0, while these accumulations were not observed in H-TS3. BCAAs are known to control bacteroid development and their accumulation under severe stress could have contributed to the high nodulation of B-TS3.

Image Guidance for Ventricular Shunt Surgery: An Analysis of Hospital Charges

Neurosurgery ◽  
2019 ◽  
Vol 85 (4) ◽  
pp. E765-E770 ◽  
Author(s):  
Sonia Ajmera ◽  
Mustafa Motiwala ◽  
Nickalus R Khan ◽  
Lydia J Smith ◽  
Kim Giles ◽  
...  
Keyword(s):  
Revision Surgery ◽  
Image Guidance ◽  
De Novo ◽  
Hospital Charges ◽  
Shunt Revision ◽  
Postanesthesia Care Unit ◽  
Shunt Surgery ◽  
Clinical Scenarios ◽  
The Difference ◽  
The Cost

Abstract BACKGROUND Image guidance for shunt surgery results in more accurate proximal catheter placement. However, reduction in shunt failure remains unclear in the literature. There have been no prior studies evaluating the cost effectiveness of neuronavigation for shunt surgery. OBJECTIVE To perform a cost analysis using available hospital charges of hypothetical shunt surgery performed with/without electromagnetic neuronavigation (EMN). METHODS Hospital charges were collected for physician fees, radiology, operating room (OR) time and supplies, postanesthesia care unit, hospitalization days, laboratory, and medications. Index shunt surgery charges (de novo or revision) were totaled and the difference calculated. This difference was compared with hospital charges for shunt revision surgery performed under 2 clinical scenarios: (1) same hospital stay as the index surgery; and (2) readmission through the emergency department. RESULTS Costs for freehand de novo and revision shunt surgery were $23 946.22 and $23 359.22, respectively. For stealth-guided de novo and revision surgery, the costs were $33 646.94 and $33 059.94, a difference of $9700.72. The largest charge increase was due to additional OR time (34 min; $4794), followed by disposable EMN equipment ($2672). Total effective charges to revise the shunt for scenarios 1 and 2 were $34 622.94 and $35 934.94, respectively. The cost ratios between the total revision charges for both scenarios and the difference in freehand vs EMN-assisted shunt surgery ($9700.72) were 3.57 and 3.70, respectively. CONCLUSION From an economic standpoint and within the limitations of our models, the number needed to prevent must be 4 or less for the use of neuronavigation to be considered cost effective.

Betaine accumulation suppresses the de-novo synthesis of ectoine at a low osmotic concentration in Halomonas sp SBS 10, a bacterium with broad salinity tolerance

2019 ◽  
Vol 46 (5) ◽  
pp. 4779-4786 ◽  
Author(s):  
Bijayendra Kushwaha ◽  
Indrani Jadhav ◽  
Hriday Narain Verma ◽  
Anjali Geethadevi ◽  
Deepak Parashar ◽  
...  
Keyword(s):  
Salinity Tolerance ◽  
De Novo ◽  
De Novo Synthesis ◽  
Osmotic Concentration
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