Effects of phages on the gut microbiota and their impact on probiotic efficacy in broiler chickens

2020 ◽  
Author(s):  
Geok Hun Tan ◽  
Mohd Shaufi Mohd Asrore ◽  
Abdul Rahman Omar ◽  
Chun Wie Chong
Keyword(s):  
Body Weight ◽  
Gut Microbiota ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Poultry Production ◽  
Feed Conversion ◽  
Villus Height ◽  
Gene Expressions ◽  
Serum Triglycerides

Abstract Background Probiotics are the most recognised alternative to antibiotic growth promoters (AGPs) for poultry production. They adhere to the intestinal epithelial cells to exert their beneficial effects on the host. However, this is often restricted by indigenous gut microbes that compete for adhesion sites and nutrients. Therefore, phage application has been proposed to reduce the competition of probiotics with gut microbiota. The main objective of this study was to investigate the effects of phages on the gut microbiota and their impact on probiotic efficacy in chickens. Results Four best Escherichia coli lytic phages were selected and characterised. The in vivo trial was conducted to investigate the effects of dietary treatments of phage cocktail at 0.1% (1ɸ) and 0.2% (2ɸ), 0.1% PrimaLac ® probiotic (P) and their combinations (1ɸP, 2ɸP) on growth performance and gut microbiota of chickens. The results from the in vivo trial showed that chickens in the 1ɸP group had significantly (P < 0.05) better body weight (BW), body weight gain (BWG), and feed conversion ratio (FCR), lower serum triglycerides and higher ileal villus height than the control. Chickens in the 1ɸP group also had higher (P < 0.05) ileal villus height, and lower crypt depth than those in the probiotic group. The gut microbiota profiles revealed that 1ɸP supplementation significantly (P < 0.05) reduced E. coli population, increased (P < 0.001) short chain fatty acids (SCFAs) producers, and improved (P < 0.001) gene expressions that are related to carbohydrate and amino acid metabolisms, and nutrient uptake. Conclusions The results from this study showed that 1ɸP treatment could modulate the gut microbiota and enhance growth performance in chickens. This finding could be considered as a potential alternative to AGPs for poultry.

scholarly journals Effect of dietary supplementation of seaweed (Ulva lactuca) and Azolla on growth performance, haematological and serum biochemical parameters of Aseel chicken

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Vijayalingam Thavasi Alagan ◽  
Rajesh Nakulan Vatsala ◽  
Ilavarasan Sagadevan ◽  
Vairamuthu Subbiah ◽  
Venkataramanan Ragothaman
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Well Being ◽  
Ulva Lactuca ◽  
Feed Conversion ◽  
Serum Biochemical

Abstract Background An effort was made to assess the effectiveness of dietary supplementation of Ulva lactuca (U. lactuca), a seaweed and Azolla individually and in combination on different physiological parameters of Aseel chicken. A total of 40 Aseel chicks of 8 weeks old were allocated into four groups, C1 (control), T1 (Azolla alone), T2 (seaweed alone) and T3 (seaweed plus Azolla). Aseel chicks in C1 were fed with formulated grower feed alone, birds in T1 had control diet with 5% Azolla, birds in T2 had control diet with 3% U. lactuca and birds in T3 had control diet admixtured with 5% of Azolla and 3% of U. lactuca. Parameters were recorded for a continuous period of 2 months in 15 days interval. Results The growth performance was found to be significant (P ≤ 0.05) during 30 days of feed trials and highly significant (P ≤ 0.01) during 45 and 60 days of treatment. Birds in T3 had a higher body weight gain, shank length and feed conversion efficiency followed with T1 and T2 compared to C1. PCV, RBC, WBC and heterophil counts were not influenced (P ≥ 0.05) by dietary treatments. Uric acid, creatinine, AST, glucose, triglycerides and magnesium levels revealed a high significant (P ≤ 0.01) variation, and cholesterol level showed significant (P ≤ 0.05) changes during 30th and 60th day of feed trials between the control and treatment groups. Total protein, globulin, phosphorus and electrolytes like Na, K and Cl levels were not significant (P ≥ 0.05) during 30th day and were highly significant (P ≤ 0.01) during the 60th day of the trial. Conclusions It could be noted that inclusion of U. lactuca and Azolla as feed supplement in grower chicken had a better body weight gain when given in combination (U. lactuca and Azolla) rather than supplemented with the sole entity. Based on the haematological and serum biochemical analysis, the supplementation of U. lactuca and Azolla at the levels included in this trial did not pose any threat to the physiological well-being of Aseel chicken.

scholarly journals Growth Performance of Clarias gariepinus on the Basis of Formulated Feed Supply

2020 ◽  
pp. 32-36
Author(s):  
Jay Narayan Shrestha ◽  
Prabha Niraula
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Protein Level ◽  
Crude Protein ◽  
Body Weight Gain ◽  
Clarias Gariepinus ◽  
Feed Conversion ◽  
Formulated Feed ◽  
Crude Protein Level

 Best diet of the fish is the sole of production of aquaculture. Growth performance of Clarias gariepinus on the basis of formulated feed supply was carried out from 7th March to 6th May, 2019 in the laboratory of P.G.Campus, Biratnagar in which 130 juveniles (each of 10±0.11 g.) of catfish were cultured in 13 aquaria feeding with treatment diets having crude protein level 35%, 40%, 45% and 50% for treatment and commercial diet for control. There are no significant differences between the water quality parameters and body weight gain (p>0.05) in all treatments. The highest body weight gain (7.31±0.11 g; 65.20%) and SGR (13.051±1.15 g) was observed in fish fed with T C (45% protein level). This value was lowered than value of control(C) i.e. 7.59±0 g; 69.88% and 13.55±0 g respectively. Feed conversion ratio (FCR) was best (13.28±1.89 g) in T D (50% protein level feed). Its value was larger (7.52±1.11 g) in T C than C (7.15±0 g). Mortality rate was highest in T C and C i.e. 30% and lowest in T A and T B i.e. 23.33%. Weight gain of the fish increased with increasing protein level in diet till crude protein level 45% then it was dropped at the diet with protein level 50%. The best weight gain was observed in 7th week having crude protein level 45%. The diet with protein level 45% is suggested for farmers concerning the fish (Clarias gariepinus) in the aquaculture.

scholarly journals Hatchability, Growth Performance, Feed Efficiency, and Survival of Normal Feathered Local, Sasso-rir and Their F1-cross Chickens Under on-station Management Condition in Southern Ethiopia

2021 ◽  
Author(s):  
shewangizaw woltamo wolde ◽  
Tadele Mirkena ◽  
Aberra Melesse ◽  
Tadelle Dessie ◽  
Solomon Abegaz
Keyword(s):  
Body Weight ◽  
Survival Rate ◽  
Growth Performance ◽  
Feed Efficiency ◽  
Body Weight Gain ◽  
The Body ◽  
Southern Ethiopia ◽  
Feed Conversion ◽  
Egg Hatchability ◽  
F1 Cross

Abstract The Normal Feathered local chicken (LL), Sasso-RIR (SRSR) and their F1-cross (LSR) chickens were hatched to evaluated for egg hatchability, growth performance, feed efficiency and survival rate. After 14-days of brooding, 150 chicks of each genotype were randomly selected and further replicated in to five pens in a deep litter grower house consisting of 30 chicks each in a completely randomized design, and evaluated for a period of 16-weeks. Hatchability of fertile eggs was highest for LL (80.0 %), intermediate for LSR (68.6%) and lowest for SRSR (55.9%) chickens. The body weight (BW) of chicks at 2-weeks of age was 80.0, 76.3 and 61.5 g/bird for SRSR, LSR, and LL, respectively, the latter being the lowest (p<0.05). The respective BW at 8-weeks of age was 732, 587 and 451 g while at 18-weeks it was 1877, 1379 and 1070 g/bird and different from each other (p<0.05). During 3- to 8-weeks and 9- to 18-weeks growth periods, the LL chickens were inferior (p< 0.05) in feed intake (29.7 and 66.9 g/d/bird) whereas the SRSR chickens were superior (p< 0.05) in body weight gain (15.5 and 16.3 g/d/bird) and feed conversion ratio (2.67 and 5.35 g feed/ g gain), respectively. The mortality rate of chicken was not affected by genotypes. It can be concluded that the exotic blood of Sasso-RIR chicken had played a significant role in upgrading the growth rate and market weight of the local Normal Feathered chicken without adverse effect on hatchability, feed efficiency, and survival rate.

scholarly journals Effect of methionine supplementation on the growth performance of rabbit

2013 ◽  
Vol 42 (1) ◽  
pp. 40-43 ◽  
Author(s):  
S Yesmin ◽  
ME Uddin ◽  
R Chacrabati ◽  
M Al-Mamun
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Control Group ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Methionine Supplementation ◽  
Four Levels

The present study was conducted to evaluate the effect of different levels of methionine supplementation on feed intake, nutrient digestibility and growth performance of growing rabbit. Sixteen weaned crossbred New Zealand White (NZW) growing rabbits (30-35 d) were distributed into four treatment groups having four replications in each group using a Completely Randomized Design (CRD). Basal diet composed of green grass (dhal grass) and concentrate mixture which was offered ad libitum basis for 56 days period. Four levels of methionine such as 0.0% (control), 0.15%, 0.25%, and 0.35% were supplemented randomly to rabbits. Results showed that supplementation of methionine did not affect green grass intake. Cumulative as well as daily concentrate and DM intake were significantly (p<0.05) higher for all methionine groups than control group. Final body weight gain as well as daily, weekly and cumulative body weight gains were improved significantly with increasing level of methionine. It was found that methionine had significant (p<0.01) effect on digestibility of DM, CP, NFE and EE but CF digestibility did not differ significantly. Digestibility was improved with increasing the level of methionine. Feed conversion ratio also decreased significantly with methionine supplementation, and 0.25% methionine group showed the best performance among the four treatments. DOI: http://dx.doi.org/10.3329/bjas.v42i1.15777 Bang. J. Anim. Sci. 2013. 42 (1): 40 43

scholarly journals Growth performance, haematological and biochemical indices of grazed Red Sokoto bucks supplemented with dried ripe plantain peels.

2020 ◽  
Vol 47 (2) ◽  
pp. 264-270
Author(s):  
A. I. Ukanwoko ◽  
N. C. Douglas ◽  
N. C. Igwe
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Conversion Ratio ◽  
The Body ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Blood Profile ◽  
Biochemical Indices

This study was conducted to evaluate the growth performance and blood profile of grazedRed Sokoto bucks supplemented with dried ripe plantain peels. Nine bucks were used for thisexperiment. The goats were apportioned to three treatment diets and dried ripe plantainpeels incorporated at 0%, 5% and 10% inclusion levels for T , T and T respectively. The 1 2 3,bucks were taken out for grazing in the morning (9.00am – 12noon) and thereafter fed theexperimental diet. The experiment lasted for eight (8) weeks. The animals were weighed atthe beginning of the experiment and weekly afterwards. Feed intake was calculated as thedifference between feed served and refusal. Blood samples of the bucks were collected todetermine the blood profile using disposable syringes and sterile needles. Results showedthat the final body weight, body weight gain and feed conversion ratio (FCR) differedsignificantly (P<0.05). The final body weight, body weight gain and FCR were 18.02kg,2.01kg, 3.42; 20.75kg, 3.89kg, 1.26 and 21.35kg, 4.80kg, 1.10 for T , T and T respectively. 1 2 3,Platelet, Alkaline phosphatase (ALP), total protein (TP), urea and creatinine values differedsignificantly (P<0.05). The highest platelet was recorded for bucks on T (212.91x10 3/ul) 3while the lowest was for bucks on T (202.30x103/ul). ALP, TP, urea and creatinine values 1were 29.00u/l, 60.00g/l, 8.00m/mol,72.50m/mol; 30.30u/l, 52.30g/l, 10.00m/mol,141.00m/mol and 42.00u/l, 53.30g/l, 9.70m/mol, 112.30m/mol for T , T and T , respectively. 1 2 3The inclusion of supplemental dried ripe plantain peels in the diet of Red Sokoto bucksenhanced the body weight and did not have any adverse effect on their blood profile.

scholarly journals Effect of feeding high yielding fodders on growth performance of growing Hilly Brown Bengal goat

2021 ◽  
pp. 73-81
Author(s):  
MM Rahman ◽  
NR Sarker ◽  
MA Alam
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Feed Intake ◽  
Body Weight Gain ◽  
Average Daily Gain ◽  
Feed Conversion ◽  
Treatment Groups ◽  
Natural Grass ◽  
Daily Gain

This study was carried out to evaluate the feeding effect of high yielding fodders (HYF) on feed intake and growth performance of growing Hilly Brown Bengal (HBB) goat. For this purpose, a feeding trail was conducted with 16 growing HBB kids (4 to 5 months) by dividing equally in four groups having four replicates for a period of 75 days. The goats in group T0 (control) received natural grass along with 101.30g concentrates and adlibitum cowpea hay, whereas in group T1, T2 and T3, only natural grass was replaced by BLRI Napier 3, BLRI Napier 4 and Ruzi fodder, respectively. Total dry matter intake (DMI), roughages DMI, crude protein intake (CP), body weight gain (kg), average daily gain (g) and forth night body weight gain (g/day) were studied. Results revealed that, DMI (288.39, 285.71, 293.48 and 301.35 g/day in group T0, T1, T2 and T3, respectively), body weight gain (3.60, 3.74, 3.73 and 3.77 kg in group T0, T1, T2 and T3, respectively), average daily gain (47.32, 49.16, 49.12 and 48.92 g in group T0, T1, T2 and T3, respectively) and feed conversion ratio (6.2, 5.9, 6.0 and 6.1 in group T0, T1, T2 and T3, respectively) of HBB kids were not differed significantly (P>0.05) for all the treatment groups. The DMI from roughages (196.2, 193.5, 201.3 and 209.2 g/day in group T0, T1, T2 and T3, respectively) and CP intake (38.7, 42.1, 48.3 and 38.8 g/day in group T0, T1, T2 and T3, respectively) were significantly varied (P<0.05), while highest CP intake was found in the treatment group T2. Forthnight body weight (g/day) was not significantly (P>0.05) gained with feeding of HYF for all the treatment groups. From this study, it can be concluded that, growing HBB kids could equally be supplied with either BLRI Napier 3 or BLRI Napier 4 or Ruzi fodder in addition to ad libitum cowpea hay for better voluntary feed intake and growth. Bang. J. Livs. Res. Vol. 27 (1&2), 2020: P. 73-81

scholarly journals Performance Chickens Kedu, Arab and Its Cross Breeds (Poncin) Of Distribution Content Protein Of Growth Fase (Age 0 -12 Week)

2020 ◽  
Vol 5 (2) ◽  
pp. 40-47
Author(s):  
I. Irmawaty ◽  
Tuti Widjastuti ◽  
Asep Anang ◽  
Muhammad Nur Hidayat
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Protein Content ◽  
Body Weight Gain ◽  
Feed Conversion ◽  
Random Design ◽  
Dietary Consumption

This experiment shows performance chickens of Kedu, Arab, and Poncin with Giffen different protein content.  This experiment uses chickens of Kedu, Arab, and Poncin (a crossbreed of male Arab and female Kedu) that each breed consists of 40 Day Old Chicken. Each species was randomly placed in 8 units of experiment cage, and every experiment cage consists of 5 Day Old Chicken.  Treatment dietary is used 15% (R1) dan 18% (R2).  The experiment's design is used a completely random design 2x3 factorial, that i two treatments of dietary and three breeds of chickens. Every treatment is replayed four times, and the show used 24 units of experiment cage.  The parameter is limited watch on dietary Consumption, body weight gain, and feed conversion. The result showed that Poncin chickens gave better growth performance when compared to Arab and Kedu chickens. Simultaneously, the ration protein content of 18 % resulted in better growth performance compared to 15% ration protein.

scholarly journals Anti-obesity effect of Tamarindus indicus seed extract against a high-fat diet-induced obese model in rats

2020 ◽  
Vol 11 (2) ◽  
pp. 2083-2089
Author(s):  
Nabeel K ◽  
Asra Fathima ◽  
Farhath Khanum ◽  
Manjula S N ◽  
Mruthunjaya K ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Body Weight Gain ◽  
Seed Extract ◽  
The Body ◽  
High Fat ◽  
Cell Viability Assay ◽  
Serum Triglycerides

The present study was aimed to evaluate the anti-obesity property of Tamarindus indica seed extract (TSE) on high fat-fed obese rats. TSE was prepared by cold maceration method and qualitative phytochemical studies had been carried out. In vitro cell viability assay (MTT assay) was and oil red staining for evaluating the lipid accumulation in cells was carried out using 3T3-L1 cells, and leptin levels was evaluated by ELISA. In-vivo Obesity was induced in experimental rats by administration of a high-fat diet for 04 weeks. The anti-obesity effect was screened by oral administration of TSE at two different dose levels i.e., 250 and 500mg/kg b. Wt. Along with a high-fat diet for a period of 04 weeks. The anti-obesity activity is estimated in terms of body weight gain, serum triglycerides (TG), Total cholesterol (TC). In -vitro studies revealed that the TSE has no cytotoxic effect, Administration of a high-fat diet for 04 weeks significantly increased the body weight, serum triglycerides, cholesterol. Upon treatment with TSE, a significant dose-dependent alteration in body weight, triglycerides, cholesterol levels were observed, inferring the anti-obesity property of Tamarindus seed extract.

scholarly journals Effects of Low-Protein Diets and Exogenous Protease on Growth Performance, Carcass Traits, Intestinal Morphology, Cecal Volatile Fatty Acids and Serum Parameters in Broilers

Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 226 ◽  
Author(s):  
Gervais Ndazigaruye ◽  
Da-Hye Kim ◽  
Chang-Won Kang ◽  
Kyung-Rae Kang ◽  
Yong-Jin Joo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Growth Performance ◽  
Relative Weight ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Intestinal Morphology ◽  
Poultry Production ◽  
Feed Conversion ◽  
Low Protein

Dietary exogenous proteases (ENZ) can be used in poultry production to improve the growth of chickens fed low-protein (LP) diets. We hypothesized that ENZ supplemented in an LP diet would improve growth performance and physiological response in broilers for 8–35 days. To investigate this, we used a 2 × 2 factorial design with crude protein (CP, normal diet (NP) and LP) and ENZ. The LP diet contained low in 1% CP and ca. 8–12% amino acids compared to the NP diet and both NP and LP diets were added without or with (1 g/kg of diet) ENZ. We randomly allocated 720 1-week-old Ross 308 male chicks to 48 pens and experimental diets. At 21 days, dietary ENZ, but not CP, increased (p = 0.007) live body weight. Body weight gain from 8–21 days was affected (p = 0.006) by dietary ENZ, but was not affected (p = 0.210) by CP. The feed conversion ratio was affected by both CP and ENZ during the starter period (p < 0.05), by ENZ (p = 0.034) during the finisher period, and by CP (p < 0.001) during the whole period. However, the interaction between CP and ENZ did not significantly affect growth performance (p > 0.05). Dietary ENZ increased (p = 0.013) the relative weight of liver at 21 days. CP and ENZ affected (p = 0.043) total short-chain fatty acids at 21 days. However, this effect was not seen (p = 0.888) at 35 days. Dietary CP increased (p < 0.05) the serum concentrations of both uric acid and creatinine in broilers. We concluded that dietary ENZ is more beneficial to younger broilers, independent of CP levels, and that its effect was restricted to body weight and the feed conversion ratio.

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