scholarly journals A Comparative study on Efficacy of Lutein and Atorvastatin on Lipid Profile and Lipoprotein(A) in Hypercholesterolemic male Wistar Rats

2021 ◽  
Vol 14 (01) ◽  
pp. 503-511
Author(s):  
Soundarya Priyadharsini K ◽  
Mali Kalpana Ramanna ◽  
Somu L ◽  
Krishna Prasad T
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Treated Group ◽  
Diet Group ◽  
Lipoprotein A ◽  
Group I ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Progression Of Atherosclerosis

Background Hypercholesterolemia is the predominantfactor in developing atherosclerosis and myocardial diseases.A major contributor for the progression of atherosclerosis is abnormalities in lipid and lipoprotein metabolism.Hence the objectives of the study was to estimatethe comparative efficacy of Lutein with atorvastatin on lipid profile and lipoprotein(a) and to estimate the histopahthological changes in hypercholesterolemic male wistar rats. Materials and Methods Experimental Wistar rats (male) were grouped into six. Each group contains 6 rats. Group I is control. Group II received cholesterol rich diet. Group III received cholesterol rich diet and the drug Atorvastatin 5mg/kg. Group IV received cholesterol rich diet and the drugLutein 25mg/kg. Group V received cholesterol rich diet and the drugLutein 50mg/kg. Group VI received cholesterol rich diet and the drug Lutein100mg/kg. At the end of 16 weeks, Blood samples from each rats was taken through retro-orbital puncture to evaluate serum lipoproteinsand lipoprotein(a) and thenwistar rats were sacrificed underinjection I.M Ketamine,Aortaand Liverwere dissected out and sent for histopathological studies. Results The plasma LDL, VLDL, Triglycerides, total cholesterol, lipoprotein(a) levels were reduced in all lutein treated groups and atorvastatin treated group compared to high cholesterol diet group. A significant rise in HDL levels was noted in all Lutein treated groups and atorvastatin treated group. No statistically significant difference was seen between Atorvastatin 5mg/kg body weight and Lutein 100mg/kg body weight on reduction of total cholesterol.The efficacy of the drug Lutein in progression of atherosclerosis and its cytoprotective action in liver was proved in this study. Conclusion This study indicates that Lutein has effect onreducing plasma lipoproteins&the study had shown significant antiatherogenic effect.

scholarly journals Melatonin menurunkan berat badan tetapi tidak menurunkan kadar TNF-α pada tikus wistar jantan yang diberi minyak jelantah selama 28 hari

2017 ◽  
Vol 5 (2) ◽  
pp. 127-132
Author(s):  
Anugrah Novianti ◽  
Edi Dharmana ◽  
Nyoman Suci Widyastiti
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Waste Cooking Oil ◽  
The Body ◽  
Control Group ◽  
Cooking Oil ◽  
Tnf Α ◽  
Significant Difference ◽  
Male Wistar Rats

Backgound: Non Alcoholic Fatty Liver Disease (NAFLD) occurs when the intake and free fatty acid synthesis occurs more frequently than its oxidation and resecretion in the blood. Melatonin is a powerful antioxidant that can boost the synthesis of endogenous antioxidants in the body, suppress the inflammatory response and inhibit the formation of steatosis.Objective: To analyze the effect of melatonin supplementation in reducing body weight andTNF-α levels in male Wistar rats were fed by waste cooking oil.Methods: True experimental studyusing post-test only control group design. This study was done on 18 male wistar rats were divided into 3 groups : the positive control group (P0) was administrated waste cooking oil, the treated group 1 (P1) was administrated waste cooking oil and 5mg/kgBW melatonin, and the treated group 2 (P2) was administrated waste cooking oil and 10mg/kgBW melatonin for 28 days.Data analysis using One Way ANOVA test and followed by Tukey test to determine the most effective dose of melatonin.Results: There was significant difference in body weightbetween P2group and K0 group (p=0,019) with the mean body weight difference was 19,167g lower than K0 group. There was no difference in TNF-α levels between the three groups (p=0,155). Conclusion: Melatonin dose of 10mg/kgBWloses body weight male Wistar rats have given by waste cooking oil for 28 days, but does not reduce TNF-α levels.

scholarly journals Effects of proportional high-protein/low-carbohydrate formulated diet consumption in diabetic rats: Beneficial impact on glycemic and weight control

2020 ◽  
Vol 20 (07) ◽  
pp. 16984-16996
Author(s):  
MMC Anyakudo ◽  
◽  
DO Adeniji ◽  
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Morphological Features ◽  
High Protein ◽  
Control Group ◽  
Formulated Diet ◽  
Low Carbohydrate ◽  
Male Wistar Rats ◽  
And Control

The metabolic response to nutrient ingestion and the rate of digestion and absorption of nutrient molecules in bowel physiology plays an important role in the metabolic control of some human chronic non-infectious diseases. This experimentally-controlled designed nutritional study which lasted eight weeks aimed to determine the effects of proportional high-protein/low-carbohydrate (HP/LC) formulated diet on glycemic tolerance, glycemic control, body weight, organ weight and organ morphometry in healthy and diabetic adult male Wistar rats. Twenty-four male Wistar rats purchased from a disease-free stock were randomly categorized into four groups (n = 6, each) after two weeks acclimatization period in raised stainless steel cages with 6 mm2mesh floor and replaceable numbered blotters papers placed under each cage in a well-ventilated animal house. Animal groups include: Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT. The animals were fed according to the experimental design with water ad libitumfor eight weeks. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bw, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly, while oral glucose tolerance test was conducted on the last day of the eighth-week study and subsequently followed by organs extraction after anesthesia for weight and gross assessment. Proportional high-protein/low-carbohydrate formulated diet caused significant reduction in mean body weight of treated diabetic (DT: 22.6%; P= .001) and healthy (HT: 5.8%; P= .007) rats while the control animals on control diet recorded significant (P< .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). Glycemic tolerance and control improved significantly in diabetic treated rats over that of the healthy treated rats. Gross morphometry of the extracted organs (kidneys, liver, heart, lungs, spleen and testes) revealed sustained normal morphological features without any visible lesion. In conclusion, consumption of proportional high-protein/low-carbohydrate formulated diet enhanced body weight reduction and sustained normal organ morphological features with good glycemic tolerance and control in experimental rats, suggesting its dietary potentiality, safety and suitability to ameliorate obesity-related diabetes.

scholarly journals Effect of Kefir, Yacon (Smallanthus sonchifolius) and the Association Between Yacon and Kefir on Intestinal Physiology in Rats With Colon Cancer

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 309-309
Author(s):  
Neuza Costa ◽  
Keila Zanardi ◽  
Caroline Woelffel ◽  
Andre Costa ◽  
Mirelle Viana ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Intestinal Permeability ◽  
Wistar Rats ◽  
Short Chain Fatty Acids ◽  
Diet Group ◽  
Standard Diet ◽  
Smallanthus Sonchifolius ◽  
Intestinal Physiology ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract Objectives To evaluate the effect of consumption of yacon flour, kefir and the association between them on colon cancer induced by 1,2-dimethylhydrazine (DMH) in male Wistar rats, SCFA production, fecal pH and intestinal permeability. Methods The study was conducted with 60 adult Wistar rats divided into 5 groups. For 5 weeks, groups T, Y, K and YK received 1,2-dimethylhydrazine (DMH) to induce colon cancer. After 5 weeks of DMH administration, animals in groups C and T received the standard diet, group Y received a diet with yacon flour with 5% FOS, group K received 1mL /day of kefir and the YK group received a diet with yacon and kefir, for 15 weeks. After euthanasia, intestinal lesions, intraluminal pH, short-chain fatty acids (SCFA) and intestinal permeability were analyzed. Results An increase in macroscopic lesions was observed in groups K (58%) and YK (42%) and a reduction of 5% in group Y, compared to group T. In addition, an increase in neoplastic changes was observed in all groups compared to group T: Y (33%), K (67%) and YK (78%). There was no significant difference in the concentrations of acetate and proprionate, pH, lactulose and mannitol between groups, and butyrate was not found in the samples. Conclusions The consumption of yacon flour, kefir and their association did not influence intestinal physiology and promoted the worsening of the development of colon carcinogenesis in rats. Funding Sources FAPES - Fundação de Amparo à Pesquisa e Inovação do Espírito Santo CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico

scholarly journals Protective Effect of Terminalia arjuna Against DBTC Induced Pancreatic Cancer in Male Wistar Rats

2021 ◽  
pp. 248-256
Author(s):  
Kadiyala Harshitha ◽  
Uma Sankar Gorla
Keyword(s):  
Pancreatic Cancer ◽  
Body Weight ◽  
Protective Effect ◽  
Wistar Rats ◽  
Negative Control ◽  
Terminalia Arjuna ◽  
Hydroalcoholic Extract ◽  
Group I ◽  
Male Wistar Rats ◽  
Per Oral

Aims: To study the protective effect of hydroalcoholic bark extract of Terminalia arjuna against DBTC induced pancreatic cancer in male wistar rats. Study design: Healthy male Wistar Albino rats weighing 150-200 g were segregated into four groups (n=6). Group I was considered as normal control, received normal saline (0.9%w/v, 1 ml/kg body weight, orally). Group II rats were treated with DBTC (6 mg/kg body weight, i.p.) which served as negative control. Group III and IV received Terminalia arjuna Linn bark hydroalcoholic extract at doses of 250 mg/kg body weight, per oral and 500 mg/kg body weight, per oral respectively. Place and Duration of Study: University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India, between May 2020 and July 2020. Methodology: The experimental animals were segregated into four groups of six rats each. According to acute toxicity data, 250 mg/kg as low dose and 500 mg/kg as high dose of the test compound have been chosen for administration. All the drugs were given for 28 consecutive days to all the respective groups with standard pellet diet and water ad libitum. The assessment of serum parameters such as α-Amylase, Lipase and blood glucose levels were carried out on 1st day, 14th day and 28th day to the respective groups. Results: Pretreated groups of Terminalia arjuna Linn bark hydroalcoholic extract (250 mg/kg and 500 mg/kg body weight, orally) showed significant (‘#’p<0.001) decrease in the levels of α-Amylase, Lipase and glucose in the blood when compared to DBTC (6 mg/kg body weight, i.p.) induced group which served as negative control. Conclusion: This study suggests that Terminalia arjuna may have a protective role against DBTC induced pancreatic cancer in male wistar rats and further investigation may be required to confirm its therapeutic potentials clinically.

scholarly journals Bioefficiency of Chromolaena odorata (Linn.) on hematological and lipid profiles in sublethal cyanide poisoning in male wistar rats

2020 ◽  
Vol 12 (1) ◽  
pp. 13-18
Author(s):  
Adeola. Folashade Ehigie ◽  
Gbadebo Emmanuel Adeleke ◽  
Fiyinfoluwa. D. Ojeniyi ◽  
Leonard Ona Ehigie
Keyword(s):  
Lipid Profile ◽  
Blood Cells ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Chromolaena Odorata ◽  
Sodium Thiosulphate ◽  
Cyanide Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Impact

Chromolaena odorata is an invasive plant which is acclaimed to have cyanide remediation potential from contaminated sites. This examination means to decide the impact of ethanol concentrates of C. odorata (ECO), sodium thiosulphate and a mix of both on hematological parameters and blood lipid profile of rodents presented to potassium cyanide. (KCN). A sum of thirty five male Wistar rats partitioned into seven groups of five units were used. KCN Group rats were administered with KCN alone. Rats in 100ECO, 150ECO, 200ECO groups were administered with 100, 150 and 200 mg/kg body weight of ECO respectively. Rats under Na2sS2O3 and Na2S2O3+ECO groups were administered 200 mg/kg sodium thiosulphate and sodium thiosulphate with ECO at 200 mg/kg respectively. The trial was done in about a month. Toward the finish of the investigation, the packed cell volume (PCV), hemoglobin level (Hb), Red blood cells (RBC) and white blood cells (WBC) were resolved utilizing known biochemical methodology. The outcomes demonstrated a noteworthy increment (p ? 0.05) in PCV, Hb, RBC and WBC level of remedial groups when contrasted with the cyanide group. Total cholesterol (TC), and triglyceride (TG) were altogether lower while HDL-cholesterol was fundamentally expanded in all the treated groups when contrasted and the untreated group given cyanide alone. However, no significant difference in LDL-cholesterol was indicated in all therapeutic groups compared with the cyanide group. The study revealed that C. odorata at the tested doses was able to improve the hematological parameters and lipid profile in cyanide exposed rats.

scholarly journals Effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats

2021 ◽  
Author(s):  
Michael A. Olamoyegun ◽  
Folasade O. Ajao ◽  
Marcus O. Iyedupe
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Restricted Feeding ◽  
Mean Values ◽  
Male Wistar Rats ◽  
Ad Libitum ◽  
Obese Male

Abstract Background: Obesity greatly increases the risk of metabolic diseases and preventive approaches for obesity are often inadequate to effectively prevent and manage the diseases. Altering feeding time strategy intervention decreases caloric intake without calorie counting and may be an effective therapy. Therefore, this study investigates the effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats. Methods: Rats placed on time-restricted feeding (TRF) regimen had freely access to food for 4 hour per day at designated periods. Twenty four rats divided into four groups (n=6) were used. Group I animals were placed on a 4 hour per day TRF between 8am-12noon. Group II rats were also placed on a 4 hour per day TRF between 12noon-4pm. Group III rats also placed on a 4 hour per day TRF between 8pm-12 midnight while Group IV rats had access food and water ad libitum. This diet strategy resembles taking only breakfast, lunch or dinner once a day. The study lasted for a period of 4 weeks with daily food intake and weekly body weight determined throughout the period. At the end of the experimental period, blood glucose, lipid profile and leptin concentration were assessed. SPSS 21.0 package was used for data analysis, one-way analysis of variance (ANOVA) was used to compare the mean values of variables among the groups and bonferroni’s posthoc test was used for significance of pair wise comparisons of mean values among the groups. Significance was set at p < 0.05.Results: In this study, the body weights and leptin concentrations of 8pm – 12am and ad libitum groups significantly increased compared with the 8am - 12noon and 12noon -4pm groups. Dyslipidemia was observed in the ad libitum group when compared with the 8am - 12noon and 12noon - 4pm groups. Conclusion: From this study, 4-hr time restricted feeding has beneficial effects on body weight, blood glucose, lipid profile and leptin concentration. This feeding restriction patterns may be helpful in obesity management and in preventing metabolic diseases development in non obese.

scholarly journals To compare the blood pressure and heart rate during course of various types of anesthesia in wistar rat: A novel experiences

2018 ◽  
Vol 9 (6) ◽  
pp. 37-39
Author(s):  
Jagdish Narayan ◽  
Pradeep Kumar ◽  
Ankit Gupta ◽  
Sunita Tiwari
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Body Weight ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Medical Sciences ◽  
The Awakening ◽  
Group I ◽  
Male Wistar Rats ◽  
Thiopental Sodium

Background: Rats are commonly used animals in development of newer drugs, rectification of toxicity and to record the various alterations in physiological parameters following pharmacological and non pharmacological interventions.Aim and Objectives: The purpose of this study was to identify the best physiological window during anesthesia. Therefore, we compared the effect of anesthesia using combination of ketamine and xylazine (KX) and thiopental sodium (intraperitoneally) on blood pressure and heart rate in adult male Wistar rats. Material and Methods: Twelve, male Wistar rats with a mean body weight of 260 ± 15 g were acquired. Thiopental sodium and cocktail of ketamine and xylazine (KX) were administered (ip) in group- I and group-II respectively. The systolic blood pressure and heart rate was recorded in both the groups till the awakening phase.Results: We found that there was a constant SBP and HR in Ketamine/Xylezine groups that are from 30 to 90 minutes after injection of anesthesia while this window was not observed in thiopental group.Conclusion: Our study concludes that the best time to observe the effect of newer drug during period between 30- 90 minutes after anesthesia.Asian Journal of Medical Sciences Vol.9(6) 2018 37-39

scholarly journals The Effectiveness of Binahong (Anredera cordifolia (Ten.) Steenis) Extract in Promoting Fertility in Male Wistar Rats after Exposure to Cigarette Smoke

2021 ◽  
Vol 9 (A) ◽  
pp. 123-128
Author(s):  
Achmad Ramadhan
Keyword(s):  
Body Weight ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Smoke Exposure ◽  
Male Rats ◽  
Male Rat ◽  
Cigarette Smoke Exposure ◽  
Testicular Weight ◽  
Significant Difference ◽  
Male Wistar Rats

BACKGROUND: Cigarette smoke has a variety of dangerous chemicals and free radicals that can potentially cause infertility. One of the plants that are reported to have medicinal properties and have active compounds as antioxidants is Binahong (Anredera cordifolia (Ten.) Steenis). AIM: This study aims to investigate the effectiveness of A. cordifolia leaf extract (ACLE) in increasing the fertility of male Wistar rats after exposure to cigarette smoke. METHODS: Twenty-four adult male rats were divided into six groups (age 8–10 weeks, weight 200–250 g): Group 1 (normal control) received aquades (1 ml/day) without being given cigarette smoke, Group 2 received exposure to smoke without being given ACLE, Group 3 received cigarette smoke exposure + 25 mg/kg ACLE, Group 4 received cigarette smoke exposure + 50 mg/kg ACLE, Group 5 received cigarette smoke exposure + 75 mg/kg ACLE, and Group 6 received exposure to cigarette smoke + 100 mg/kg of ACLE. To produce smoke from cigarettes and expose rats to the smoke, a Smoke chamber is used. Rats in the treatment group were exposed to cigarette smoke for 2 weeks (40–60 s daily for 6 days each week). The evaluation of male rat fertility was carried out by measuring body weight, genital weight (testis and epididymis), and spermatozoa spermogram (velocity, motility, morphology, and total number). RESULTS: The least significance different test results showed no significant difference in the rate of weight gain between treatments, standard control, and negative control groups. The average testicular weight of rats exposed to cigarette smoke for 14 days was significantly different from normal controls and doses of 50, 75, and 100 mg/kg body weight. However, at a dose of 25 mg/kg of body weight had not shown a significant increase compared to control. The average spermatozoa velocity reached 12.43 mm2/s and 13.36 mm2/s. The spermatozoa velocity increased significantly at a dose of 100 mg/kg body weight. CONCLUSIONS: ACLE at a dose of 100 mg/kg body weight was effective in increasing the fertility of male Wistar rats exposed to cigarette smoke.

scholarly journals Effects of Oral Administration of Monosodium Glutamate (MSG) on Obesity in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 5 (9) ◽  
pp. 879-882
Author(s):  
Mulyati Sri Rahayu ◽  
Sri Wahyuni ◽  
Yuziani
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Body Weight Gain ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Group 2

Introduction: Monosodium glutamate (MSG) is one of the most widely employed food enhancers. Although the umami compound, controversy persists regarding the effects of MSG intake on body weight. Chronic MSG intake may result in excessive body weight gain and obesity. Consumption of MSG result in organ damage, cardiovascular disease, oxidative stress, and also risk factors for obesity. This study aims to determine the effect of oral MSG on obesity in adult male Wistar rats (Rattus norvegicus).Methods: This true experimental study used the post-test control group design. Twenty-four adult male Wistar rats were randomly divided into four groups: control (received distilled water), Group 1 (MSG 0.378 mg/gr BW), Group 2 (0.756 mg/gr BW) and Group 3 (1.512 mg/gr BW). The obesity parameter was obtained by the Lee index. Kruskal-Wallis test follows by Mann-Whitney test were used to compare the Lee index between groups.Results: Lee’s index mean for each group was 358.4%, 314.1%, 287.8%, and 320.9%, respectively. The Kruskal Wallis test showed a significant difference in the Lee index between groups (p = 0.043). A follow-up test using Mann-Whitney found a significant difference between group 2 and the control group (p = 0.043, p <0.05). The mean of Lee index of group 2 was 70.51% lower than the control group.Conclusion: This study concluded that Lee index was not increased in MSG-treated rats than in the control group after oral MSG intervention for 21 days.

scholarly journals The effect of meniran herbs extract (Phyllantus niruri) to triglycerides blood level in wistar rats

2012 ◽  
Vol 10 (1) ◽  
pp. 23-27
Author(s):  
JUDO YUSTANTO KAHONO ◽  
KISRINI KISRINI ◽  
YUL MARIYAH
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Total Period ◽  
Group I ◽  
Significant Difference ◽  
Post Test ◽  
Group Ii

Kahono JY, Kisrini, Mariyah Y. 2012. The effect of meniran herbs (Phyllantus niruri) extract to blood triglyceride level in rats. Biofarmasi 10: 23-27. The aim of this experiment was to determine the effect of meniran herbs (Phyllantus niruri L.) extract to blood triglycerides level in Wistar rats (Rattus norvegicus). This experiment was a laboratory experiment with pre and post-test with a control group design. The samples were 30 male Wistar rats with ±200 gram of body weight and ±2 months old. The samples were divided into five groups by using a purposive random sampling technique and each group consisted of six rats. Group I as a negative control, group II as a positive control, and groups III, IV and V as meniran herbs extract groups with first dose, second dose, and third dose. All rats were simultaneously given with hyperlipemic feed. Group II was simultaneously administered with Cholvastin 0.5 mg/200 grams body weight/day. Groups III, IV and V were simultaneously treated with meniran herbs extract at dose of 25 mg, 50 mg and 100 mg/200 grams body weight/day. The total period of this experiment was 21 days. At the day 1st and 21st, blood was collected from rat orbital venous plexus. Serum was analyzed for their pre and post-test blood triglycerides. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by a post-hoc test. The value of p=0.002 (p<0.05) showed there was a significant difference between pre-test and post-test in delta means of blood triglycerides level among groups. Meniran herbs extract had a significant triglycerides lowering effect against blood triglycerides level of Wistar rats. The most potential of meniran herbs extract dose was 100 mg/200 gram body weight/day.

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