scholarly journals Phenolic compounds and biological activities of phenolic extract of olive oil mill wastewater issue from the cold extraction of olive oil from Khenchela (Algeria)

2021 ◽  
Author(s):  
Zakia Gueboudji ◽  
Kenza Kadi ◽  
Kamel Nagaz ◽  
Dalila Addad ◽  
Mansour Secrafi ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Olive Oil ◽  
Protein Denaturation ◽  
Biological Activities ◽  
Quinic Acid ◽  
Quality Analysis ◽  
Olive Oil Mill Wastewater ◽  
Reducing Ability ◽  
Natural Phenolic Compounds ◽  
Anti Inflammatory

Abstract This study aimed to the determination of antioxidant, anti-inflammatory, and anticoagulant activities of phenolic compounds present in olive oil mill wastewater (OMW) issue from the cold extraction of olive oil from Khenchela eastern of Algeria.After polyphenols extraction, a quantity and quality analysis by LC-MS was made. The LC-MS (liquid chromatography–mass spectrometry)results were revealed the presence of 20 phenolic compounds in theextract of OMW which were: (quinic acid, gallic acid, protocatechuic acid, caffeic acid, p-coumaric acid, rutin, transfrolic acid, hyperoside (quercetin-3-o-galactoside), luteolin-7-o-glucoside, naringin, 4.5-di-caffeoyquinic acid, quercetrin(quercetin-3-o-rhamonosid), apegenin-7-o-glucoside, salviolinic acid, kampherol, quercetin, naringenin, apegenin,cirsiliol, and cirsilineol). The results of antioxidant activity with DPPH(2,2-diphenyl-1-picrylhydrazyl), ABTS+ (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), and FRAP (ferric reducing ability of plasma)showed that the IC50(half-maximal inhibitory concentration) were(9.62 ± 0.28µg/mL, 7.10 ± 0.11µg/mL and 3.59 ± 0.24µg/mL)respectively. The extract of OMW was found to exhibit the highestinhibitory effectanti-inflammatory activity usinginhibition of protein denaturation(IPD) and membrane stabilizing potential (MSP) tests (80.46 ± 3.81; 87.43 ± 0.66 µg/mL) more than the standard used. In addition, the extract had the best anticoagulation activity in the endogenous and exogenous pathways (44.77 ± 0.25s; 15.84 ± 0.12s). Based on these results, it is right to conclude that OMW is an important source of natural phenolic compounds that have important antioxidant, anti-inflammatory, and anticoagulant activities.

Fibrinolytic, Anti-Inflammatory and Cytotoxic Potentialities of Extracts and Chemical Constituents of Manglicolous Lichen, Graphis ajarekarii Patw. & C. R. Kulk

2020 ◽  
Vol 10 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Vinay Bharadwaj Tatipamula ◽  
Girija Sastry Vedula
Keyword(s):  
Protein Denaturation ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Fibrin Clot ◽  
Hydroalcoholic Extract ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

Background: Lichens which are betide to mangroves are termed as Manglicolous Lichens (ML). As these ML are habituated under stress conditions, they are screened for unique metabolites and biological activities. Objective: The study aimed to establish the chemical and biological profile of ML, Graphis ajarekarii. Methods: The Ethyl Acetate Extract of G. ajarekarii (EAE) was subjected to chromatographic techniques and the obtained isolates were characterized by spectroscopic analysis. The hydroalcoholic extract of G. ajarekarii (AE), EAE, isolates and Hydroalcoholic Extract of host (HE) were evaluated for fibrinolytic (fibrin clot method), in vitro (protein denaturation method) and in vivo (formalin-induced rat paw oedema assay), anti-inflammatory and cytotoxicity (MTT assay) activities. Results: Chemical investigation of the EAE led to the isolation of two known compounds namely atranorin (1) and ribenone (2), which were confirmed by spectral data. The AE and EAE gradually lysed the fibrin clot with 94.54 and 65.07%, respectively, at 24 h. The AE inhibited protein denaturation of about 88.06%, while the standard (Indomethacin) with 93.62%. Similarly, the in vivo antiinflammatory analysis of AE (200 mg/mL) showed potent reduction of rat paw oedema than the standard, whereas EAE and 1 depicted moderate depletion. In addition, the AE revealed prominence inhibition on MCF-7, DU145 and K-562 with IC50 values of 69.5, 42.5 and 38 µg/mL, respectively, whereas the HE exhibited mild inhibitory profile against fibrin clot, inflammation and cancer. Conclusion: From the results, it can be concluded that the G. ajarekarii has an aptitude to act against coagulation, inflammation and cancer cells.

scholarly journals Bioactive Compounds of Selected Fruit Juices

2009 ◽  
Vol 4 (5) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Aneta Wojdyłto ◽  
Jan Oszmiański
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Fruit Juice ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Sea Buckthorn ◽  
Quercetin Derivatives ◽  
Reducing Ability ◽  
Wide Range

Fruits are one of the most important sources of phenolic compounds in our diet. Many of these compounds exhibit a wide range of biological activities, especially antioxidant activity. The objective of this study was to determine the composition and antioxidant activity of phenolic compounds found in fruit juice obtained from buckthorn, flowering quince, rowanberry and hawthorn. Hydroxycinnamic acids (neochlorogenic and chlorogenic acid) and procyanidins were the main phenolics in rowanberry juice. The highest level of flavonols (quercetin derivatives) was found in sea buckthorn. All juices showed strong free-radical scavenging activity against ABTS•+ radicals and ferric reducing ability measured by the FRAP method. The strongest antioxidant activity was found in rowanberry juice, but the lowest was measured in hawthorn and sea buckthorn juices.

scholarly journals Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey

2020 ◽  
Vol 10 (5) ◽  
pp. 1845 ◽  
Author(s):  
Alexandra M. Afonso ◽  
Joana Gonçalves ◽  
Ângelo Luís ◽  
Eugenia Gallardo ◽  
Ana Paula Duarte
Keyword(s):  
Wound Healing ◽  
Phenolic Compounds ◽  
Protein Denaturation ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Natural Substances ◽  
Anti Inflammatory ◽  
Free Radical Scavenging Assay ◽  
Wound Healing Activity

Honey and propolis are natural substances produced by Apis mellifera that contain flavonoids, phenolic acids, and several other phytochemicals. The aim of this study was to phytochemically characterize three different types of honey and propolis, both separately and mixed, and to evaluate their wound-healing activity. Total phenolic compounds and flavonoids were determined using the Folin–Ciocalteu’s and aluminum chloride colorimetric methods, respectively. The antioxidant activity was evaluated by both the DPPH free radical scavenging assay and β-carotene bleaching test, and the anti-inflammatory activity was determined by a protein denaturation method. To evaluate the wound-healing activity of the samples, NHDF cells were subjected to a wound scratch assay. The obtained results showed that dark-brown honey presents a higher concentration of phenolic compounds and flavonoids, as well as higher antioxidant and anti-inflammatory activities. Propolis samples had the highest concentrations in bioactive compounds. Examining the microscopic images, it was possible to verify that the samples promote cell migration, demonstrating the wound-healing potential of honey and propolis.

scholarly journals Antioxidant and anti-inflammatory activities of winery wastes seeds of Vitis labrusca

2011 ◽  
Vol 41 (7) ◽  
pp. 1233-1238 ◽  
Author(s):  
Gustavo Scola ◽  
Virginia Demarchi Kappel ◽  
José Claudio Fonseca Moreira ◽  
Felipe Dal-Pizzol ◽  
Mirian Salvador
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Content ◽  
Biological Activities ◽  
Total Phenolic ◽  
Vitis Labrusca ◽  
Grape Seeds ◽  
Wine Production ◽  
Beneficial Effects ◽  
Environmental Balance ◽  
Anti Inflammatory

There are many studies about the biological activities of Vitis vinifera grape seeds, which are rich in phenolic compounds, known by their several health beneficial effects. However, until now there is no data about biological activities of the seeds of V. labrusca, specie found in South and North America. Every year, the global wine production (around 260 million hL) generates about 19.5 million ton of wastes, which are usually discarded in the environment. The aim of this research was to evaluate the antioxidant and anti-inflammatory activities of aqueous extracts of seeds from wine wastes of Vitis labrusca (cv. 'Bordo' and 'Isabella'). Both extracts showed significant antioxidant and anti-inflammatory activities, which are positively correlated with total phenolic content, suggesting that these compounds might be the major contributors to the biological activity of these extracts. These results indicate that water extraction from winery wastes is an option to obtain phenolic compounds with antioxidant and anti-inflammatory activities helping to maintain environmental balance.

scholarly journals BEARING REACTION OF OLIVE OIL MILL WASTEWATER IN VARIOUS LITHOLOGY ROCKS

2004 ◽  
Vol 36 (1) ◽  
pp. 236
Author(s):  
Β. Περδικάτσης ◽  
Ε. Μανούτσογλου ◽  
Ν. Σπάρταλη ◽  
Δ. Μωραϊτης ◽  
Δ. Πεντάρη
Keyword(s):  
Electrical Conductivity ◽  
Phenolic Compounds ◽  
Olive Oil ◽  
Effective Treatment ◽  
Organic Liquid ◽  
Liquid Waste ◽  
Mineralogical Composition ◽  
Olive Oil Mill Wastewater ◽  
The Common ◽  
Liquid Wastes

The olive oil production countries are currently faced with the major problem of dealing with olive oil mill wastewater. Effective treatment of these organic liquid wastes (katsigaros) has not been proposed yet. The common practice followed the last fifteen years in the island of Crete includes the creation of open wastewater evaporation ponds. The present work studies the sorption of organic agents, more specifically the phenolic compounds, in various lithology rocks. Three different samples were tested including Neogene marl-calcareous marl from Platania/Kissamou (PLA) area, schistolithic rock from Sarakina/Selinou (SAR) area and Neogene marl-siliceous (diatomite) marl successions from Basilies/Heraklion (VAS). The organic liquid wastes affect differently the PH and electrical conductivity from the studied rocks with different mineralogical composition. Rocks containing lllite show a significant adsorption of the phenolic compounds. Similar behavior is observed for the Neogene marl-siliceous (diatomite) samples, while the schistolithic rock show the lowest adsorption. The katsigaros dissolve the calcite and iron minerals and increases the CaO and Fe203 in the liquid waste.

scholarly journals Phytochemical Screening and In Vitro Antioxidant and Anti-inflammatory Activities of Aerial Parts of Euphorbia hirta L.

2021 ◽  
Vol 42 (1) ◽  
pp. 115-124
Author(s):  
Deepak Basyal ◽  
Astha Neupane ◽  
Durga Prasad Pandey ◽  
Shiva Pandeya
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Euphorbia Hirta ◽  
Anti Inflammatory

Euphorbia hirta L (Euphorbiaceae) also called asthma herb has long been prescribed in traditional medicine because it exhibits diverse pharmacological actions due to the presence of alkaloids, flavonoids, polyphenols, triterpenoids, and saponins. The present study is aimed at the study of phytochemical and antioxidant activity and anti-inflammatory screening of E. hirta. Extraction of dried powder was performed followed by phytochemical screening using color reactions. Total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were estimated by Folin-Ciocalteu and Aluminum chloride method respectively. The antioxidant activity was studied by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method.  Anti-inflammatory activity was studied by using protein denaturation in vitro bioassay. Phytochemical screening showed the presence of flavonoids, alkaloids, and phenolic compounds. TPC, TFC and antioxidant activity (IC50) of the extract were found as 288.10 mg gallic acid equivalent per gram (GAE/g), 29.36 mg quercetin equivalent per gram (QE/g) and 32.23 µg/mL (p<0.05) respectively. Diclofenac sodium and E. hirta extract showed the maximum inhibition of 91.28% and 68.20% respectively at the concentration of 1000 µg/mL compared with control (p>0.05). The phenolic compounds and flavonoids exert antioxidant and anti-inflammatory activities because of their scavenging ability. The demonstrated antioxidant and anti-inflammatory activities may be the rationale behind some of its folkloric uses and also may be responsible for some of its pharmacological effects. Thus, E. hirta can be considered a good source of antioxidants and anti-inflammatory actions, which might be beneficial for combating oxidative stress.

scholarly journals Hydroxytyrosol, Tyrosol and Derivatives and Their Potential Effects on Human Health

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 2001 ◽  
Author(s):  
Ana Karković Marković ◽  
Jelena Torić ◽  
Monika Barbarić ◽  
Cvijeta Jakobušić Brala
Keyword(s):  
Phenolic Compounds ◽  
Olive Oil ◽  
Biological Activities ◽  
In Vivo Studies ◽  
Therapeutic Effects ◽  
Endocrine Effects ◽  
Pharmacological Potential ◽  
A Minor

The Mediterranean diet and olive oil as its quintessential part are almost synonymous with a healthy way of eating and living nowadays. This kind of diet has been highly appreciated and is widely recognized for being associated with many favorable effects, such as reduced incidence of different chronic diseases and prolonged longevity. Although olive oil polyphenols present a minor fraction in the composition of olive oil, they seem to be of great importance when it comes to the health benefits, and interest in their biological and potential therapeutic effects is huge. There is a growing body of in vitro and in vivo studies, as well as intervention-based clinical trials, revealing new aspects of already known and many new, previously unknown activities and health effects of these compounds. This review summarizes recent findings regarding biological activities, metabolism and bioavailability of the major olive oil phenolic compounds—hydroxytyrosol, tyrosol, oleuropein, oleocanthal and oleacein—the most important being their antiatherogenic, cardioprotective, anticancer, neuroprotective and endocrine effects. The evidence presented in the review concludes that these phenolic compounds have great pharmacological potential, however, further studies are still required.

scholarly journals Biological Activities of Phenolic Compounds of Extra Virgin Olive Oil

Antioxidants ◽  
2013 ◽  
Vol 3 (1) ◽  
pp. 1-23 ◽  
Author(s):  
Maurizio Servili ◽  
Beatrice Sordini ◽  
Sonia Esposto ◽  
Stefania Urbani ◽  
Gianluca Veneziani ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Olive Oil ◽  
Biological Activities ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil
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