scholarly journals Highly Efficient Production of Transfructosylating Enzymes Using Low-Cost Sugarcane Molasses by A. Pullulans FRR 5284

2021 ◽  
Author(s):  
Most Sheauly Khatun ◽  
Morteza Hassanpour ◽  
Mark Harrison ◽  
Robert Speight ◽  
Ian O'Hara ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Production Process ◽  
Enzyme Production ◽  
Low Cost ◽  
Scale Up ◽  
Extracellular Enzyme ◽  
Efficient Production ◽  
Sugarcane Molasses ◽  
Shake Flasks ◽  
Different Sources

Abstract In this study, sugarcane molasses was used to produce transfructosylating enzymes by A. pullulans FRR 5284. It was found that NaNO3 was a better nitrogen source than yeast extract while exogeneous phosphorous was not needed. Adding only 4.4 g/L NaNO3 into the molasses medium containing 100 g/L sugars led to the highest total transfructosylating activity of 123.8 U/mL. Scale-up of the enzyme production process from shake flasks to 1 L reactor improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than the corresponding activity and productivity from shake flasks, respectively. FOS production from 500 g/L sucrose led to the highest yields of ~ 61% using intracellular, extracellular, and total enzymes from shake flasks and the reactor. Enzymes from different sources led to very different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios to adjust prebiotic activity.

scholarly journals Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Most Sheauly Khatun ◽  
Morteza Hassanpour ◽  
Mark D. Harrison ◽  
Robert E. Speight ◽  
Ian M. O’Hara ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Production Systems ◽  
Low Cost ◽  
Scale Up ◽  
Maximum Yield ◽  
Extracellular Enzyme ◽  
Efficient Production ◽  
Sugarcane Molasses ◽  
Transfructosylating Enzyme ◽  
Shake Flasks

AbstractFructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. NaNO3 was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of NaNO3 to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L NaNO3 into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity.

Optimal Conditions for Cholesterol Oxidase Production and Enzymatic Properties Study Produced by Monascus X-1

2012 ◽  
Vol 195-196 ◽  
pp. 469-474
Author(s):  
Lu Hao ◽  
Qian Zhang ◽  
Chang Lu Wang ◽  
Mian Hua Chen ◽  
Yu Rong Wang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Cholesterol Oxidase ◽  
Extracellular Enzyme ◽  
Optimal Conditions ◽  
Cultivation Conditions ◽  
Inoculum Concentration ◽  
Fermentation Conditions ◽  
Optimum Ph ◽  
Shake Flasks ◽  
Rice Medium

In this paper we optimized liquid fermentation conditions of Monascus X-1 on cholesterol oxidase (COD) production, and then the COD was isolated by ammonium sulfate deposition and its properties were studied. The results showed that COD produced by Monascus X-1 was a kind of extracellular enzyme. The yields came to maximum at the 6th day. The most suitable medium for Monascus X-1 generating COD was the rice medium, the highest enzyme activity was obtained at 140r/min, inoculum concentration 6%, when 60mL of the culture in 250mL shake flasks grown in rice medium. Under the optimized cultivation conditions, the enzyme activity reached 1168U/L, which increased 1.52 times compared to the previous fermentation conditions. The enzyme had favorable pH and thermal stability, while the optimum pH and temperature of enzyme activity was 7.0 and 40, respectively.

scholarly journals Statistical-Based Bioprocess Optimization of Amylase Production from Halophilic Bacillus sp. H7.

2021 ◽  
Author(s):  
J.N. Bandal ◽  
V.A. Tile ◽  
R. Z. Sayyed ◽  
H.P. Jadhav ◽  
N. I. Wan Azelee ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Amylase Activity ◽  
Scale Up ◽  
Fold Increase ◽  
Laboratory Scale ◽  
Bacillus Sp ◽  
Bioprocess Optimization ◽  
Critical Variable ◽  
Amylase Production ◽  
Shake Flasks

Using the above results from RMS analysis the optimum values were predicted for the independent significant variables (Figure 3) the optimized levels of these variables in combination with other media variables the maximum production was predicted to be 199.90 U/mL. The predicted data were validated through confirmatory experiments performed in triplicates. A 1.29-fold increase in amylase activity against un-optimized (OVAT) medium was achieved in the present study authenticating the efficacy of RSM in process optimization (Figure 4). 2.6 Model validation and scale-up at laboratory scale (5L) bioreactor Once the parameters were standardized in the shake-flasks culture, the experiment was scaled-up to a laboratory-scale bioreactor (5 L). The yield of amylase increased by 1.01 fold (205.69 U/mL), it could be possible because the enzyme production in a bioreactor is higher than in shake-flasks culture as the various critical variable factors such as the dissolved oxygen (DO) and the pH can be optimally controlled at the desired levels [22].

Screening and statistical optimization of media ingredients for production of microbial transglutaminase

2017 ◽  
Vol 2 (2) ◽  
pp. 216 ◽  
Author(s):  
Janifer Raj Xavier ◽  
K. V. Ramana ◽  
R. K. Sharma
Keyword(s):  
Enzyme Activity ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Incubation Temperature ◽  
Scale Up ◽  
Industrial Applications ◽  
Acyl Transfer ◽  
Cottage Cheese ◽  
Sugarcane Molasses ◽  
Transglutaminase Activity

<p>Transglutaminase is a calcium dependent enzyme that catalyses acyl transfer reactions between primary amino groups and protein bound glutamine residues. Eighteen bacterial and twenty eight actinomycetes were screened for the presence of transglutaminase. Among the microbial cultures screened <em>Streptomyces</em> sp. D1, showed maximum transglutaminase activity. In this study characterization of transglutaminase and its application to modifying the properties of panner (Indian cottage cheese) in the form of cross linking was investigated. Optimum temperature and pH for enzyme was found to be at 50°C and 6.0, respectively. Optimization of media ingredients for maximizing the transglutaminase activity using <em>Streptomyces</em> sp. D1 was carried out using central composite design. Response surface methodology was employed to standardize the optimum media composition for maximum enzyme activity. Three factors such as carbon source, nitrogen source and pH were tested for the maximum enzyme activity as response. The optimized medium with sugarcane molasses as carbon source 6.0% (w/v), peptone as nitrogen source 1.75% (w/v) were found to be optimal at initial pH 6.5 and incubation temperature 30.0°C with agitation at 100 rpm for 96h. The enzyme activity of transglutaminase obtained from the optimized medium was found to be 4.1 (AU/ml). Low cost substrate such as sugarcane molasses in the form of a renewable substrate is proposed to be suitable even for scale-up production of enzyme and for industrial applications. The ethanol fractionated transglutaminse treated milk was found to produce more paneer with increased moisture content while reduction in cooking loss of the paneer prepared using enzyme treated milk is also reported.</p>

scholarly journals Modelling a production process using a Sankey diagram and Computerized Relative Allocation of Facilities Technique (CRAFT)

2019 ◽  
Vol 9 (1) ◽  
pp. 444-449 ◽  
Author(s):  
Kampf Rudolf ◽  
Hlatká Martina
Keyword(s):  
Production Process ◽  
Work Environment ◽  
Low Cost ◽  
Efficient Production ◽  
Manufacturing Companies ◽  
Car Seat ◽  
Production Processes

AbstractThis paper constitutes a partial output of carried out research and is focused on modelling the production process of car seat manufacturing. Given that manufacturing companies are currently struggling with a shortage of qualified workforce, it is the job of every business to keep their current employees. Companies make efforts to operate the most efficient production processes at very low cost and with labour saving. In order to produce at low cost, the work environment must be designed efficiently. There are many ways to do this. In this paper focused on optimizing production processes, Computerized Relative Allocation of Facilities Technique (CRAFT) and a Sankey diagram will be used.

Constructive Optimisation of the Propeller Type Mixing Devices Using the Virtual and Rapid Prototyping

2017 ◽  
Vol 68 (3) ◽  
pp. 453-458 ◽  
Author(s):  
Daniel Besnea ◽  
Alina Spanu ◽  
Iuliana Marlena Prodea ◽  
Gheorghita Tomescu ◽  
Iolanda Constanta Panait
Keyword(s):  
Rapid Prototyping ◽  
Low Cost ◽  
Scale Up ◽  
Three Dimensional ◽  
Rapid Identification ◽  
Multidisciplinary Optimization ◽  
External Diameter ◽  
Process Industries ◽  
Parametric Cad ◽  
Shaft Torque

The paper points out the advantages of rapid prototyping for improving the performances/constructive optimization of mixing devices used in process industries, here exemplified to propeller types ones. The multidisciplinary optimization of the propeller profile affords its design using parametric CAD methods. Starting from the mathematical curve equations proposed for the blade profile, it was determined its three-dimensional virtual model. The challenge has been focused on the variation of propeller pitch and external diameter. Three dimensional ranges were manufactured using the additive manufacturing process with Marker Boot 3D printer. The mixing performances were tested on the mixing equipment measuring the minimum rotational speed and the correspondent shaft torque for complete suspension achieved for each of the three models. The virtual and rapid prototyping method is newly proposed by the authors to obtain the basic data for scale up of the mixing systems, in the case of flexible production (of low quantities), in which both the nature and concentration of the constituents in the final product varies often. It is an efficient and low cost method for the rapid identification of the optimal mixing device configuration, which contributes to the costs reduction and to the growing of the output.

scholarly journals Hydrogen production from water electrolysis: role of catalysts

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Shan Wang ◽  
Aolin Lu ◽  
Chuan-Jian Zhong
Keyword(s):  
Hydrogen Production ◽  
Water Splitting ◽  
Noble Metal ◽  
Active Sites ◽  
Current Knowledge ◽  
Low Cost ◽  
Critical Role ◽  
Water Electrolysis ◽  
Efficient Production ◽  
Production Of Hydrogen

AbstractAs a promising substitute for fossil fuels, hydrogen has emerged as a clean and renewable energy. A key challenge is the efficient production of hydrogen to meet the commercial-scale demand of hydrogen. Water splitting electrolysis is a promising pathway to achieve the efficient hydrogen production in terms of energy conversion and storage in which catalysis or electrocatalysis plays a critical role. The development of active, stable, and low-cost catalysts or electrocatalysts is an essential prerequisite for achieving the desired electrocatalytic hydrogen production from water splitting for practical use, which constitutes the central focus of this review. It will start with an introduction of the water splitting performance evaluation of various electrocatalysts in terms of activity, stability, and efficiency. This will be followed by outlining current knowledge on the two half-cell reactions, hydrogen evolution reaction (HER) and oxygen evolution reaction (OER), in terms of reaction mechanisms in alkaline and acidic media. Recent advances in the design and preparation of nanostructured noble-metal and non-noble metal-based electrocatalysts will be discussed. New strategies and insights in exploring the synergistic structure, morphology, composition, and active sites of the nanostructured electrocatalysts for increasing the electrocatalytic activity and stability in HER and OER will be highlighted. Finally, future challenges and perspectives in the design of active and robust electrocatalysts for HER and OER towards efficient production of hydrogen from water splitting electrolysis will also be outlined.

scholarly journals In vitro conversion of proapoprotein A-I to apoprotein A-I. Partial characterization of an extracellular enzyme activity.

1983 ◽  
Vol 258 (19) ◽  
pp. 11430-11433 ◽  
Author(s):  
C Edelstein ◽  
J I Gordon ◽  
K Toscas ◽  
H F Sims ◽  
A W Strauss ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Extracellular Enzyme ◽  
Extracellular Enzyme Activity ◽  
Partial Characterization

scholarly journals Recent Findings in Azaphilone Pigments

2021 ◽  
Vol 7 (7) ◽  
pp. 541
Author(s):  
Lúcia P. S. Pimenta ◽  
Dhionne C. Gomes ◽  
Patrícia G. Cardoso ◽  
Jacqueline A. Takahashi
Keyword(s):  
Water Solubility ◽  
Biological Activities ◽  
Low Cost ◽  
Scale Up ◽  
Structural Diversity ◽  
Downstream Processing ◽  
Global Market ◽  
Yield Improvement ◽  
Potential Applications ◽  
New Compounds

Filamentous fungi are known to biosynthesize an extraordinary range of azaphilones pigments with structural diversity and advantages over vegetal-derived colored natural products such agile and simple cultivation in the lab, acceptance of low-cost substrates, speed yield improvement, and ease of downstream processing. Modern genetic engineering allows industrial production, providing pigments with higher thermostability, water-solubility, and promising bioactivities combined with ecological functions. This review, covering the literature from 2020 onwards, focuses on the state-of-the-art of azaphilone dyes, the global market scenario, new compounds isolated in the period with respective biological activities, and biosynthetic pathways. Furthermore, we discussed the innovations of azaphilone cultivation and extraction techniques, as well as in yield improvement and scale-up. Potential applications in the food, cosmetic, pharmaceutical, and textile industries were also explored.

Sign in / Sign up

Export Citation Format

Share Document