Arthrospira platensis Mediated Biosynthesis of Silver Nanoparticles as Breast Cancer Proliferation and Differentiation Controlling agent: In-vitro and In-vivo Safety Approach
Abstract Biogenic Silver nanoparticles (AgNPs) are one of the most fascinating nanomaterials used in biomedical purposes. In the current study, we biosynthesized AgNPs using Arthrospira platensis, Microcystis aeruginosa and Chlorella vulgarisactive metabolites and evaluate their efficacy against breast cancer. The recovered AgNPs was characterized using scanning and transmission electron microscopy (SEM and TEM). The safety usage of bio-AgNPs was tested in-vitro on PBMCs cells and in-vivo. The obtained results indicated the safety usage of bio-AgNPs at concentration 0.1 mg/ml on PBMCs cells and 1.5mg/ml on the tested Albino mice. The bio-AgNPs displayed dose-dependent cytotoxicity (0.1 mg/ml) against HepG-2, CaCO-2 and MCF-7 cell lines via ROS induction and arresting treated cells in G0/G1 and sub G0 phases. In addition, Arthrospira bio-AgNPs treatment induced cellular apoptosis in breast cancer cells via the down regulation of survivin, MMP7, TGF and Bcl2 genes expressions. Upon Arthrospira bio-AgNPs treatment, a great delay in tumor growth and prolonged survival were recorded in breast cancer mice model. Furthermore, after Arthrospira bio-AgNPs treatment, a great reduction in Ki 67 protein marker from 60% to be 20% and was recorded and an elevation in Caspase 3 protein levels was recorded in treated groups with percentage 65% comparing with 45% in Doxorubicin treated groups.