scholarly journals Cervicovaginal COVID-19 Positivity: A Pilot Study

2021 ◽  
Author(s):  
Barış Çiplak ◽  
Yavuz Şimşek ◽  
Mustafa Kara ◽  
Rukiye Akyol ◽  
Lokman Hizmali ◽  
...  
Keyword(s):  
Reproductive Tract ◽  
Sexual Transmission ◽  
Reproductive Organs ◽  
Female Reproductive Tract ◽  
Nasopharyngeal Swab ◽  
Swab Sample ◽  
Rt Pcr ◽  
Viral Nucleic Acid ◽  
Cervical Swab ◽  
Cervicovaginal Fluid

Abstract Purpose: ACE 2 RNA expression has been detected in organs of the female reproductive tract, suggesting that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could potentially infect female reproductive organs. In this study, we investigated the presence of SARS-CoV-2 virus in the cervicovaginal fluid.Materials and Methods: Our study included 31 female patients aged 18–65 years. The presence of SARS-COV-2 RNA was investigated by RT-PCR in two separate cervicovaginal swab samples collected from patients 14 days apart. Viral RNA was extracted using viral nucleic acid buffer (vNAT) solution, and SARS-COV-2 RNA was analyzed using Bio-speedy SARS-CoV-2 RT-qPCR kits in Bio-Rad CFX96 TouchTM device.Results: The first and second cervical swab samples were collected from 22 of 31 patients 14 days apart. The first cervical swab sample was collected from 9 patients; however, the second swab sample could not be collected after 14 days. SARS-COV-2 RNA result was negative in 100% of a total of 53 cervicovaginal swab samples collected. Moreover, the SARS-COV-2 RNA result was negative in the nasopharyngeal swab of babies after delivery in three pregnant women.Conclusion: Negative SARS-COV-2 RNA results in cervicovaginal swab samples suggest that there is no sexual transmission of COVID-19 and no vertical transmission during pregnancy. However, the number of studies conducted on this subject and the sample size examined are still insufficient.

Female reproductive organs procurement v1

2021 ◽  
Author(s):  
kone not provided
Keyword(s):  
Reproductive Tract ◽  
Deceased Donor ◽  
Reproductive Organs ◽  
Female Reproductive Tract ◽  
Pregnant Female ◽  
Female Reproductive Organs

Description of procurement of non-pregnant female reproductive tract from deceased donor for HuBMAP.

scholarly journals In vitro 3D Model of Female Reproductive Tract: An Overview and Future Aspect

2021 ◽  
Author(s):  
Arnaud Martino Capuzzo
Keyword(s):  
Reproductive Tract ◽  
Three Dimensional ◽  
Reproductive Organs ◽  
Experimental Models ◽  
Female Reproductive Tract ◽  
Complex Interactions ◽  
Current State ◽  
Pregnancy And Delivery ◽  
Recent Developments

Hormones must be balanced and dynamically controlled for the Female Reproductive Tract (FRT) to function correctly during the menstrual cycle, pregnancy, and delivery. Gamete selection and successful transfer to the uterus, where it implants and pregnancy occurs, is supported by the mucosal epithelial lining of the FRT ovaries, uterus, cervix, fallopian tubes, and vagina. Successful implantation and placentation in humans and other animals rely on complex interactions between the embryo and a receptive female reproductive system. The FRT's recent breakthroughs in three-dimensional (3D) organoid systems now provide critical experimental models that match the organ's physiological, functional, and anatomical characteristics in vitro. This article summarizes the current state of the art on organoids generated from various parts of the FRT. The current analysis examines recent developments in the creation of organoid models of reproductive organs, as well as their future directions.

scholarly journals Major Critical Periods in Developmental Pathogenomics of Endometriosis

2019 ◽  
Vol 3 (1) ◽  
pp. 01-02
Author(s):  
Vladislav Baranov
Keyword(s):  
Molecular Mechanisms ◽  
Reproductive Tract ◽  
Benign Tumor ◽  
Reproductive Organs ◽  
Genetic Program ◽  
Female Reproductive Tract ◽  
Critical Periods ◽  
Uterine Endometrium ◽  
Common Benign Tumor ◽  
Major Genome

In spite on numerous experimental and clinical data molecular mechanisms of endometriosis (EM) - the most common benign tumor of the female reproductive tract still remains obscure . The deciphering enigmas of EM gave a birth to a number of hypothesis . System genetics approach used in our studies of common diseases support the existence of special genetic program of EM operative in its development. It is taken for granted that EM results from abnormal differentiation of stem cells (SC). Two major sources of EM SC are considered : SC disseminated throughout peritoneum during female reproductive organs embryogenesis , SC from junction zone the uterine endometrium (2) [6]. According to our reviewed hypothesis [7] the genetic program of EM consists of several critical periods (CP) [8] corresponding to three crucial events in EM development with each of them corresponding to major genome reprogramming in EM cells.

scholarly journals Characterization of the Influence of Semen-Derived Enhancer of Virus Infection on the Interaction of HIV-1 with Female Reproductive Tract Tissues

2015 ◽  
Vol 89 (10) ◽  
pp. 5569-5580 ◽  
Author(s):  
Shannon A. Allen ◽  
Ann M. Carias ◽  
Meegan R. Anderson ◽  
Eneniziaogochukwu A. Okocha ◽  
Lorie Benning ◽  
...  
Keyword(s):  
Cell Culture ◽  
Virus Infection ◽  
Reproductive Tract ◽  
Sexual Transmission ◽  
Female Reproductive Tract ◽  
Productive Infection ◽  
Target Cells ◽  
Mucosal Transmission ◽  
Epithelial Barriers ◽  
Hiv 1

ABSTRACTThe majority of human immunodeficiency virus type 1 (HIV-1) transmission events occur in women when semen harboring infectious virus is deposited onto the mucosal barriers of the vaginal, ectocervical, and endocervical epithelia. Seminal factors such as semen-derived enhancer of virus infection (SEVI) fibrils were previously shown to greatly enhance the infectivity of HIV-1 in cell culture systems. However, when SEVI is intravaginally applied to living animals, there is no effect on vaginal transmission. To define how SEVI might function in the context of sexual transmission, we applied HIV-1 and SEVI to intact human and rhesus macaque reproductive tract tissues to determine how it influences virus interactions with these barriers. We show that SEVI binds HIV-1 and sequesters most virions to the luminal surface of the stratified squamous epithelium, significantly reducing the number of virions that penetrated the tissue. In the simple columnar epithelium, SEVI was no longer fibrillar in structure and was detached from virions but allowed significantly deeper epithelial virus penetration. These observations reveal that the action of SEVI in intact tissues is very different in the anatomical context of sexual transmission and begin to explain the lack of stimulation of infection observed in the highly relevant mucosal transmission model.IMPORTANCEThe most common mode of HIV-1 transmission in women occurs via genital exposure to the semen of HIV-infected men. A productive infection requires the virus to penetrate female reproductive tract epithelial barriers to infect underlying target cells. Certain factors identified within semen, termed semen-derived enhancers of virus infection (SEVI), have been shown to significantly enhance HIV-1 infectivity in cell culture. However, when applied to the genital tracts of living female macaques, SEVI did not enhance virus transmission. Here we show that SEVI functions very differently in the context of intact mucosal tissues. SEVI decreases HIV-1 penetration of squamous epithelial barriers in humans and macaques. At the mucus-coated columnar epithelial barrier, the HIV-1/SEVI interaction is disrupted. These observations suggest that SEVI may not play a significant stimulatory role in the efficiency of male-to-female sexual transmission of HIV.

scholarly journals Head-to-head comparison of SARS-CoV-2 antigen-detecting rapid test with self-collected anterior nasal swab versus professional-collected nasopharyngeal swab

2020 ◽  
Author(s):  
Andreas K. Lindner ◽  
Olga Nikolai ◽  
Franka Kausch ◽  
Mia Wintel ◽  
Franziska Hommes ◽  
...  
Keyword(s):  
Point Of Care ◽  
Rapid Test ◽  
High Viral Load ◽  
Ease Of Use ◽  
Healthcare Personnel ◽  
Nasopharyngeal Swab ◽  
Swab Sample ◽  
Rt Pcr ◽  
Percent Agreement ◽  
Accuracy Study

AbstractBackgroundTwo antigen-detecting rapid diagnostic tests (Ag-RDTs) are now approved through the WHO Emergency Use Listing procedure and can be performed at the point-of-care. However, both tests use nasopharyngeal (NP) swab samples. NP swab samples must be collected by trained healthcare personnel with protective equipment and are frequently perceived as uncomfortable by patients.MethodsThis was a manufacturer-independent, prospective diagnostic accuracy study with comparison of a supervised, self-collected anterior nose (AN) swab sample with a professional-collected NP swab sample, using a WHO-listed SARS-CoV-2 Ag-RDT, STANDARD Q COVID-19 Ag Test (SD Biosensor), which is also being distributed by Roche. The reference standard was RT-PCR from an oro-/nasopharyngeal swab sample. Percent positive and negative agreement as well as sensitivity and specificity were calculated.ResultsAmong the 289 participants, 39 (13.5%) tested positive for SARS-CoV-2 by RT-PCR. The positive percent agreement of the two different sampling techniques for the Ag-RDT was 90.6% (CI 75.8-96.8). The negative percent agreement was 99.2% (CI 97.2-99.8). The Ag-RDT with AN sampling showed a sensitivity of 74.4% (29/39 PCR positives detected; CI 58.9-85.4) and specificity of 99.2% (CI 97.1-99.8) compared to RT-PCR. The sensitivity with NP sampling was 79.5% (31/39 PCR positives detected; CI 64.5-89.2) and specificity was 99.6% (CI 97.8-100). In patients with high viral load (>7.0 log 10 RNA SARS-CoV2/swab), the sensitivity of the Ag-RDT with AN sampling was 96% and 100% with NP sampling.ConclusionSupervised self-sampling from the anterior nose is a reliable alternative to professional nasopharyngeal sampling using a WHO-listed SARS-CoV-2 Ag-RDT. Considering the ease-of-use of Ag-RDTs, self-sampling and potentially patient self-testing at home may be a future use case.

scholarly journals Dynamic expression of bone morphogenetic protein 4 in reproductive organs of female mice

Reproduction ◽  
2011 ◽  
Vol 142 (4) ◽  
pp. 573-579 ◽  
Author(s):  
Pradeep S Tanwar ◽  
James R McFarlane
Keyword(s):  
Bone Morphogenetic Protein ◽  
Granulosa Cells ◽  
Reproductive Tract ◽  
Follicular Development ◽  
Reproductive Organs ◽  
Bmp Signaling ◽  
Female Reproductive Tract ◽  
Specific Staining ◽  
Primordial Follicles ◽  
Morphogenetic Protein

Various members of the bone morphogenetic protein (BMP) family have been shown to regulate mammalian follicular development by affecting granulosa cell proliferation and steroidogenesis.In situhybridization studies have shown expression of BMPR1A, BMPR1B, and BMPR2 in the granulosa cells and oocyte of most of the follicles in the ovary, suggesting that these cells have the capacity to respond to BMP signaling. Although much is known about BMP4 signaling, its expression pattern in the female reproductive tract (FRT) is still unclear. The objective of the current study was to characterize the expression of BMP4 and its downstream target proteins (pSMAD1/5/8) in the FRT. In the ovary, BMP4 protein was detected in all the stages of follicular development. Staining for pSMAD1/5/8 was observed in granulosa cells and oocytes of all the stages of follicular development including primordial follicles, suggesting that these follicles are responsive to autocrine/paracrine BMP signaling. In the uterus, BMP4 and pSMAD1/5/8 staining was observed in all three compartments and strongest expression was observed during the estrus phase. BMP4- and pSMAD1/5/8-specific staining was also observed in oviductal epithelium. Different forms (apparent MW: 50, 35, and 15 kDa) of BMP4 were detected in mouse ovary by western blot analysis. In conclusion, these results have defined BMP4 and pSMAD1/5/8 protein expression in the mouse FRT and highlighted the importance of BMP4 in folliculogenesis.

Gross and ultrasonographic morphometry of female reproductive tract in small ruminants

2018 ◽  
Vol 52 (1-4) ◽  
pp. 31-38 ◽  
Author(s):  
R Jannat ◽  
F Y Bari ◽  
R N Ferdousy ◽  
M Hassan ◽  
N S Juyena
Keyword(s):  
Reproductive Tract ◽  
Small Ruminants ◽  
Reproductive Organs ◽  
Female Reproductive Tract ◽  
Model Study ◽  
Linear Probe ◽  
Measurement Results ◽  
Disease Condition ◽  
Length Width ◽  
The Mean

Understanding the anatomy of female reproductive organs is very much important to identify any variation in disease condition. Therefore, this study was conducted to determine the gross and ultrasonographic morphometric of female reproductive tract in small ruminants. The reproductive tracts of 21 does and 20 ewes were collected from slaughter house and both gross and ultrasonographic image measurements were performed to study morphometric of cervix, body of uterus, horn of uterus and ovary. Water bath ultrasonography technique was used with trans-abdominal linear probe for image measurement. Results revealed significant (P<0.001) variation between gross and image measurements of cervix, body of uterus and ovaries in does. In ewes, the significant (P<0.001) variation was observed between gross and image measurements in diameter of ovaries. Gross measurements were proportionately higher than image measurements in both species. The mean length, width and diameters of right ovaries were found higher than those of left ovaries. Pearson’s correlation revealed a positive relation between two measurements. Moreover, it was found that echogenicity varied with reproductive organs. This is a model study, which may help to identify female reproductive structures in small ruminants when trans-abdominal probe is used.

Expression of NK1 receptor at the protein and mRNA level in the porcine female reproductive system

2014 ◽  
Vol 17 (3) ◽  
pp. 469-477
Author(s):  
R. Bukowski
Keyword(s):  
Blood Vessels ◽  
Reproductive System ◽  
Reproductive Tract ◽  
Mrna Level ◽  
Female Reproductive Tract ◽  
Receptor Protein ◽  
Nk1 Receptor ◽  
Female Reproductive System ◽  
Rt Pcr ◽  
Arterial Blood

Abstract The presence and distribution of substance P (SP) receptor NK1 was studied in the ovary, the oviduct and the uterus (uterine horn and cervix) of the domestic pig using the methods of molecular biology (RT-PCR and immunoblot) and immunohistochemistry. The expression of NK1 receptor at mRNA level was confirmed with RT-PCR in all the studied parts of the porcine female reproductive system by the presence of 525 bp PCR product and at the protein level by the detection of 46 kDa protein band in immunoblot. Immunohistochemical staining revealed the cellular distribution of NK1 receptor protein. In the ovary NKI receptor was present in the wall of arterial blood vessels, as well as in ovarian follicles of different stages of development. In the tubular organs the NK1 receptor immunohistochemical stainings were observed in the wall of the arterial blood vessels, in the muscular membrane, as well as in the mucosal epithelium. The study confirmed the presence of NK1 receptor in the tissues of the porcine female reproductive tract which clearly points to the possibility that SP can influence porcine ovary, oviduct and uterus.

scholarly journals Ammonium transporter expression in sperm of the disease vectorAedes aegyptimosquito influences male fertility

2020 ◽  
Vol 117 (47) ◽  
pp. 29712-29719
Author(s):  
Andrea C. Durant ◽  
Andrew Donini
Keyword(s):  
Reproductive Tract ◽  
Reproductive Organs ◽  
Female Reproductive Tract ◽  
Ammonium Transporter ◽  
Ammonia Toxicity ◽  
Mrna Levels ◽  
Acid Base Balance ◽  
Domains Of Life ◽  
Base Balance ◽  
Rh Proteins

The ammonium transporter (AMT)/methylammonium permease (MEP)/Rhesus glycoprotein (Rh) family of ammonia (NH3/NH4+) transporters has been identified in organisms from all domains of life. In animals, fundamental roles for AMT and Rh proteins in the specific transport of ammonia across biological membranes to mitigate ammonia toxicity and aid in osmoregulation, acid–base balance, and excretion have been well documented. Here, we observed enrichedAmt(AeAmt1) mRNA levels within reproductive organs of the arboviral vector mosquito,Aedes aegypti, prompting us to explore the role of AMTs in reproduction. We show that AeAmt1 is localized to sperm flagella during all stages of spermiogenesis and spermatogenesis in male testes. AeAmt1 expression in sperm flagella persists in spermatozoa that navigate the female reproductive tract following insemination and are stored within the spermathecae, as well as throughout sperm migration along the spermathecal ducts during ovulation to fertilize the descending egg. We demonstrate that RNA interference (RNAi)-mediated AeAmt1 protein knockdown leads to significant reductions (∼40%) of spermatozoa stored in seminal vesicles of males, resulting in decreased egg viability when these males inseminate nonmated females. We suggest that AeAmt1 function in spermatozoa is to protect against ammonia toxicity based on our observations of high NH4+levels in the densely packed spermathecae of mated females. The presence of AMT proteins, in addition to Rh proteins, across insect taxa may indicate a conserved function for AMTs in sperm viability and reproduction in general.

Distribution of Trimethoprim and Sulfametopyrazine in the Female Reproductive Tract

1986 ◽  
Vol 14 (2) ◽  
pp. 101-104 ◽  
Author(s):  
Paulo Cesar Preza ◽  
Claudia Moura ◽  
Aguinaldo Leite Filho ◽  
Valeria Cavallari ◽  
Franklin Nüdemberg ◽  
...  
Keyword(s):  
Uterine Leiomyoma ◽  
Reproductive Tract ◽  
Female Genital Tract ◽  
Reproductive Organs ◽  
Female Reproductive Tract ◽  
Bacterial Strains ◽  
Dose Ratio ◽  
Reproductive Tract Infections ◽  
Tract Infections ◽  
Female Genital

A combination of trimethoprim (TMP) 250 mg and sulfametopyrazine (SMP) 200 mg (dose ratio 5:4) in capsules was administered to ten patients undergoing hysterectomy for uterine leiomyoma. Each patient received a total of 3 of these Kelfiprim capsules: 2 capsules in a single administration 36 hours before surgery and 1 capsule 24 hours later. TMP and SMP concentrations were analyzed in plasma and also in the ovary, Fallopian tube and uterus, removed during surgery. The results indicated that in the reproductive organs of the female genital tract TMP reaches the same concentrations as in plasma. In contrast SMP is 3 to 3·5 times less concentrated in these tissues compared with plasma. At the TMP/SMP ratios obtained in these various organs synergism of the two compounds is reported to be particularly prominent for most bacterial strains. These findings indicate that this TMP-SMP combination may be useful in the treatment of female reproductive tract infections.

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