scholarly journals Nanosilver toxicity thresholds estimative for aquatic and sediment environmental safety

2021 ◽  
Author(s):  
Vera Lucia Castro ◽  
Claudio Jonsson ◽  
Mariana Silveira Guerra M. Silva ◽  
Rodrigo Castanha ◽  
Jose Henrique Vallim ◽  
...  
Keyword(s):  
Culture Media ◽  
Aquatic Insect ◽  
Environmental Safety ◽  
Artemia Salina ◽  
Logistic Function ◽  
Environmental Concentration ◽  
Management Measures ◽  
Effect Concentration ◽  
Aquatic Compartment ◽  
Expected Increase

Abstract Silver nanoparticles (AgNPs) have been largely utilized. Despite that, comprehensive studies on their ecotoxicological effects and environmental risk are still required. In the present study, the AgNPs effect was assessed to some organisms including algae, plants, microcrustaceans, cnidaria, nematodes, aquatic insect, earthworm, and fish embryos. The no-observed-effect concentration (NOEC) was calculated for each organism using a log-logistic function. AgNPs remained stable in contact with culture media during the analyzed period and conditions employed, presenting dispersion less than 20%, except for Artemia salina medium. On this occasion, NPs presented dispersion of approximately 25%, although their size remained unchangeable. The Predicted No Effect Concentration (PNEC) of AgNPs in the aquatic compartment was estimated in the concentration range from 0.13 to 0.66 µg L-1. A Risk Quotient (RQ) of 15.1 was derived for the NP tested with the aid of a maximum AgNPs Predicted Environmental Concentration (PEC) estimated value. The RQ value superior to one indicates a risk and the need for its management measures implementation. These data, in addition to the expected increase in AgNPs use, reinforce the importance of the AgNPs safety levels establishment that can contribute to performing their risk assessment studies.

scholarly journals Predictive ecological risk assessment of pesticide mixtures in Mediterranean porous aquifers

2018 ◽  
Vol 1 ◽  
Author(s):  
Tiziana Di Lorenzo ◽  
Marco Cifoni ◽  
Barbara Fiasca ◽  
Alessia Di Cioccio ◽  
Diana Galassi
Keyword(s):  
Ecological Risk ◽  
Concentration Addition ◽  
Toxicity Data ◽  
Model Species ◽  
Risk Quotient ◽  
Environmental Concentration ◽  
Pesticide Mixtures ◽  
Abruzzo Region ◽  
Effect Concentration ◽  
Porous Aquifers

The ecological risk assessment (ERA) of chemical substances is based on the premise that the protection of the most sensitive taxon safeguards the overall community. Given the severe scarcity of ecotoxicological data concerning groundwater species, we felt urged to consider epigean model species’ sensitivity data to determine the safe pesticide concentrations for obligate groundwater dwelling species. To this end, we performed the ERA of pesticide mixtures occurring in eleven Mediterranean porous aquifers (Abruzzo region; central Italy). The evaluation was based on data collected between 2010 and 2015 by the environmental protection agency of Abruzzo region (ARTA Abruzzo) and included 42 pesticides and 1953 samples. We applied a step-wise procedure: we used the Measured Environmental Concentration (MEC); we estimated the Predicted No-Effect Concentration (PNEC) and we established the ecological risk as Risk Quotient (RQ) based on the ∑MECi/PNECi ratio following a concentration addition model for mixtures’ toxicity. we used the Measured Environmental Concentration (MEC); we estimated the Predicted No-Effect Concentration (PNEC) and we established the ecological risk as Risk Quotient (RQ) based on the ∑MECi/PNECi ratio following a concentration addition model for mixtures’ toxicity. The PNEC was regarded as the concentration below which a harmful effect will most likely not occur to the groundwater dwelling fauna. The toxicity data used to compute the PNEC values were obtained from the US. EPA ECOTOX database confining the search to epigean crustaceans. Missing toxicity data were estimated by ECOSAR v.1.11. PNEC values were calculated by dividing the lowest short-term L(E)C50 value (that refers to the concentration at which 50% of its maximal effect was observed in test species) by appropriate assessment factors (AF). The AF values were selected according to the difference in the sensitivities of groundwater and epigean crustaceans derived from the available studies. Groundwater crustacean species were generally less sensitive to acute exposure to chemicals than the model species Daphnia magna. However, they were more sensitive than their epigean relatives when the comparisons were made among organisms sharing the same family/order. This result suggests caution when inferring the sensitivity of groundwater species from that of epigean taxa. The ecological risk was scored using a binary ecological classification suggesting that appreciable risk is likely when RQ≥1. Pesticide mixture risks were often driven by a minimum of 2 to 11 compounds in the eleven aquifers of Abruzzo region. However, the risk-drivers (i.e., individual pesticides explaining the largest share of potential effects) differed substantially among the aquifers. The results of this study have been published by Di Lorenzo et al. (2018).

scholarly journals Sea Bass Immunization to Downsize the Betanodavirus Protein Displayed in the Surface of Inactivated Repair-Less Bacteria

Vaccines ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 94
Author(s):  
Lama ◽  
Pereiro ◽  
Novoa ◽  
Coll
Keyword(s):  
Culture Media ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Surface Expression ◽  
Environmental Safety ◽  
Nervous Necrosis Virus ◽  
Necrosis Virus ◽  
European Sea Bass ◽  
Viral Nervous Necrosis ◽  
Casein Hydrolysates

: This work describes immunization of European sea bass (Dicentrarchus labrax) juveniles against viral nervous necrosis virus (VNNV), a betanodavirus causing worldwide mortalities in many fish species. Protection was obtained with the so-called spinycterin vehicles consisting of irreversibly DNA-damaged DNA-repair-less Escherichia coli displaying at their surface a downsized VNNV coat antigen. In this work we have i) maximized bacterial expression levels by downsizing the coat protein of VNNV to a fragment (frgC91–220) containing most of its previously determined antigenicity, ii) developed a scalable autoinduction culture media for E.coli based in soy-bean rather than in casein hydrolysates, iii) enriched surface expression by screening different anchors from several prokaryotic sources (anchor + frgC91–220 recombinant products), iv) preserved frgC91–220 antigenicity by inactivating bacteria by irreversible DNA-damage by means of Ciprofloxacin, and v) increased safety using a repair-less E.coli strain as chassis for the spinycterins. These spinycterins protected fish against VNNV challenge with partial (Nmistic + frgC91–220) or total (YBEL + frgC91–220) levels of protection, in contrast to fish immunized with frgC91–220 spinycterins. The proposed spinycterin platform has high levels of environmental safety and cost effectiveness and required no adjuvants, thus providing potential to further develop VNNV vaccines for sustainable aquaculture.

scholarly journals Antibacterial, cytotoxic and trypanocidal activities of marine-derived fungi isolated from Philippine macroalgae and seagrasses

2018 ◽  
Vol 77 (2) ◽  
pp. 141-151 ◽  
Author(s):  
Kin Israel Notarte ◽  
Takashi Yaguchi ◽  
Keisuke Suganuma ◽  
Thomas Edison dela Cruz
Keyword(s):  
Gene Sequence ◽  
Culture Media ◽  
Artemia Salina ◽  
Malt Extract ◽  
Zone Of Inhibition ◽  
Potato Dextrose Broth ◽  
Gene Sequence Analysis ◽  
Marine Salt ◽  
Mycelia Sterilia ◽  
Selection Of

AbstractThe occurrence and bioactivities of marine-derived fungi are evaluated in this paper. A total of 16 morphospecies of marine-derived fungi (MDF) were isolated from four host macroalgae and two seagrasses and identified as belonging to the generaAspergillus,Fusarium,Paecilomyces,Penicillium,Sclerotinia,ThamnidiumandTrichoderma, including fivemycelia sterilia.Among these host organisms, the rhodophyteLaurencia intermediaharboured the highest number of isolated MDF. Selected MDF were then assayed and showed to inhibitPseudomonas aeruginosa(8-19 mm zone of inhibition) andStaphylococcus aureus(6-19 mm zone of inhibition), and were cytotoxic against the brine shrimpArtemia salinanauplii (LD50: 201.56-948.37 μg mL−1). The screening led to the selection of five of the most bioactive morphospecies, all belonging to the genusAspergillus. These marine aspergilli were subjected toβ-tubulingene sequence analysis for species identification, and to mass production in different culture media with or without marine salts, and screening of the crude culture extracts for their cytotoxic and trypanocidal activities.Aspergillus tubingensiscultivated in potato dextrose broth with marine salt proved to be the most cytotoxic against P388 (IC50: 1028 ng mL−1) and HeLa (IC50: 1301 ng mL−1) cancer cells. On the other hand,A. fumigatuscultivated in malt extract broth without marine salt was shown to be the most potent againstTrypanosoma congolense(IC50: 298.18 ng mL−1). Our study therefore showed that salinity may influence the bioactivities of some species of MDF.

scholarly journals Effects of Subchronic Exposure of Diclofenac on Growth, Histopathological Changes, and Oxidative Stress in Zebrafish (Danio rerio)

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Eva Praskova ◽  
Lucie Plhalova ◽  
Lucie Chromcova ◽  
Stanislava Stepanova ◽  
Iveta Bedanova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glutathione Reductase ◽  
Danio Rerio ◽  
Fish Growth ◽  
Histopathological Changes ◽  
Glutathione S Transferase ◽  
Environmental Concentration ◽  
Subchronic Exposure ◽  
Effect Concentration ◽  
And Oxidative Stress

The aim of this study was to investigate effects of subchronic exposure to sublethal levels of diclofenac on growth, oxidative stress, and histopathological changes inDanio rerio. The juvenile growth tests were performed onDanio rerioaccording to OECD method number 215. Fish at the age of 20 days were exposed to the diclofenac environmental concentration commonly detected in the Czech rivers (0.02 mg L−1) and the range of sublethal concentrations of diclofenac (5, 15, 30, and 60 mg L−1) for 28 days. A significant decrease (P<0.01) in the fish growth caused by diclofenac was observed in the concentrations of 30 and 60 mg L−1. The identified value of LOEC (lowest observed effect concentration) was 15 mg L−1of diclofenac and NOEC (no observed effect concentration) value was 5 mg L−1of diclofenac. We did not find histopathological changes and changes of selected parameters of oxidative stress (glutathione S-transferase, glutathione reductase) in tested fish. The environmental concentration of diclofenac in Czech rivers did not have any effect on growth, selected oxidative stress parameters (glutathione S-transferase, glutathione reductase), or histopathological changes inDanio reriobut it could have an influence on lipid peroxidation.

scholarly journals Shape and rate of movement of the invasion front of Xylella fastidiosa spp. pauca in Puglia

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
David Kottelenberg ◽  
Lia Hemerik ◽  
Maria Saponari ◽  
Wopke van der Werf
Keyword(s):  
Xylella Fastidiosa ◽  
Logistic Function ◽  
Disease Spread ◽  
Buffer Zones ◽  
Invasion Front ◽  
Empirical Estimates ◽  
Rate Of Spread ◽  
Management Measures ◽  
Olive Quick Decline Syndrome ◽  
Rate Of Movement

AbstractIn 2013, Xylella fastidiosa spp. pauca was first reported in Puglia, Italy, causing the olive quick decline syndrome (OQDS). Since then the disease has spread, prompting the initiation of management measures to contain the outbreak. Estimates of the shape of the disease front and the rate of area expansion are needed to inform management, e.g. the delineation of buffer zones. However, empirical estimates of the invasion front and the rate of spread of OQDS are not available. Here, we analysed the hundreds of thousands of records of monitoring data on disease occurrence in Puglia to estimate the shape of the invasion front and the rate of movement of the front. The robustness of estimation was checked using simulation. The shape of the front was best fitted by a logistic function while using a beta-binomial error distribution to model variability around the expected proportion of infected trees. The estimated rate of movement of the front was 10.0 km per year (95% confidence interval: 7.5–12.5 km per year). This rate of movement is at the upper limit of previous expert judgements. The shape of the front was flatter than expected. The fitted model indicates that the disease spread started approximately in 2008. This analysis underpins projections of further disease spread and the need for preparedness in areas that are still disease free.

scholarly journals Perbedaan Pertumbuhan Artemia salina Pada Perlakuan Variasi Dosis Pakan Jus Pupa Ulat Sutra (Samia cynthia)

2020 ◽  
Vol 6 (1) ◽  
pp. 10-20
Author(s):  
Agus Dharmawan ◽  
Nurul Hikmah ◽  
Mita Larasati
Keyword(s):  
Culture Media ◽  
Average Length ◽  
Abiotic Factors ◽  
Artemia Salina ◽  
Dose Treatment ◽  
Silkworm Pupae ◽  
Silk Worm ◽  
Silkworm Pupa ◽  
Protein Feed ◽  
Observation Results

High protein feed are very necessary in the cultivation industry of Artemia salina. One of the proteins sources that can be used is silk worm pupae (Samia cynthia). The aim of this study was to determine the differences in growth of Artemia salina with the treatment of silkworm pupa juice feeding at various doses. This study uses 4 levels of treatment, namely 0.16 ml; 0.33 ml; 0.50 ml and 0.67 ml per liter with 6 replications, which are given daily for 21 days. Abiotic factors of culture media (salinity, temperature, pH, and DO) are controlled and in accordance with the needs of Artemia salina's life. Observation results on the 15th day, 17th day, and 19th day showed that there were differences in the number of Artemia salina due to the treatment of variations in the feed dose of silkworm pupae juice (Samia cynthia) with 0.67 ml feed dosage treatment having the highest average number and significantly different from other dosage treatments. Observations on the 13th day, 15th day and 21st day showed differences in the growth length of Artemia salina due to the variation of the dosage treatment of silkworm pupa juice (Samia cynthia) with 0.50 ml dose treatment having the highest average length and significantly different from other treatments. Keywords: Artemia salina, growth, feed, silkworm pupae ABSTRAK Pakan yang mengandung protein tinggi sangat diperlukan dalam budidaya Artemia salina. Salah satu sumber protein yang dapat digunakan adalah pupa ulat sutra (Samia cynthia). Tujuan penelitian ini untuk mengetahui perbedaan pertumbuhan Artemia salina dengan perlakuan pemberian pakan jus pupa ulat sutra pada berbagai dosis. Penelitian ini menggunakan 4 taraf perlakuan yaitu dosis 0,16 ml; 0,33 ml; 0,50 ml dan 0,67 ml per liter dengan 6 ulangan, yang diberikan setiap hari selama 21 hari. Faktor abiotik media kultur (salinitas, suhu, pH, dan DO) terkontrol dan sesuai dengan kebutuhan hidup Artemia salina. Hasil pengamatan hari ke-15, hari ke-17, dan hari ke-19 menunjukkan adanya perbedaan jumlah Artemia salina akibat pemberian perlakuan variasi dosis pakan jus pupa ulat sutra (Samia chyntia) dengan perlakuan dosisi pakan 0,67 ml memiliki retata jumlah tertinggi dan berbeda nyata dengan perlakuan dosis lainnya. Hasil pengamatan pada hari ke-13, hari ke-15 dan hari ke-21 menunjukkan adanya perbedaan pertumbuhan panjang Artemia salina akibat pemberian perlakuan variasi dosis pakan jus pupa ulat sutra (Samia chyntia) dengan perlakuan dosis 0,50 ml memiliki rerata panjang tertinggi dan berbeda nyata dengan perlakuan yang lain. Kata kunci: Artemia salina, pertumbuhan, pakan, pupa ulat sutra

scholarly journals Effect of Terbuthylazine-2-hydroxy at Environmental Concentrations on Early Life Stages of Common Carp (Cyprinus carpioL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Josef Velisek ◽  
Alzbeta Stara ◽  
Dalibor Koutnik ◽  
Jana Machova
Keyword(s):  
Common Carp ◽  
Early Life ◽  
Life Stage ◽  
Early Life Stage ◽  
Control Fish ◽  
Control Group ◽  
Embryo Viability ◽  
Sod Activity ◽  
Environmental Concentration ◽  
Effect Concentration

The aim of the study was to investigate effects of the triazine’s herbicide terbuthylazine-2-hydroxy on early life stage of common carp (Cyprinus carpioL.) through antioxidant indices, mortality, growth, development, and histopathology. Based on accumulated mortality in the experimental groups, lethal concentrations of terbuthylazine-2-hydroxy were estimated at 35-day LC50 = 10.9 mg/L terbuthylazine-2-hydroxy. By day 15, fish were exposed to 3.5 mg/L and by day 26, fish were exposed to 0.0029 mg/L; real environmental concentration in Czech rivers, 0.07 mg/L, 1.4 mg/L, and 3.5 mg/L terbuthylazine-2-hydroxy, showed significantly lower mass and total length compared with controls. Based on inhibition of growth in the experimental groups, lowest observed effect concentration (LOEC) = 0.002 mg/L terbuthylazine-2-hydroxy and no observed effect concentration (NOEC) = 0.0001 mg/L terbuthylazine-2-hydroxy. No significant negative effects on hatching or embryo viability were demonstrated at the concentrations tested, but significant differences in early ontogeny among groups were noted. Fish from the two highest tested concentrations showed a dose-related delay in development compared with the controls. Total superoxide dismutase (SOD) activity was significant lower in all groups testedly for terbuthylazine-2-hydroxy compared with the control group. At concentrations of 1.4 and 3.5 mg/L damage to caudal kidney tubules when compared to control fish was found.

scholarly journals Cytotoxic Metabolites Produced by Alternaria No.28, an Endophytic Fungus Isolated from Ginkgo biloba

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Jian-Chun Qin ◽  
Ya-Mei Zhang ◽  
Ling Hu ◽  
Ya-Tuan Ma ◽  
Jin-Ming Gao
Keyword(s):  
Ginkgo Biloba ◽  
Endophytic Fungus ◽  
Brine Shrimp ◽  
Culture Media ◽  
Monomethyl Ether ◽  
Artemia Salina ◽  
Fungal Endophyte ◽  
Alternariol Monomethyl Ether ◽  
Cytotoxic Effects

From the medicinal plant Ginkgo biloba the fungal endophyte Alternaria no.28 was isolated. Extract of the fungus grown in liquid culture media exhibited marked cytotoxic activity when tested in vitro against brine shrimp (Artemia salina). Eight compounds were isolated from the extract of cultures of this endophytic fungus and were elucidated as alterperylenol (1), altertoxin I (2), alternariol (3), alternariol monomethyl ether (4), tenuazonic acid (5) and its derivative (6), together with ergosterol and ergosta-4, 6, 8, 22-tetraen-3-one by means of spectroscopic analysis. Among them, both 5 and 6 showed significant cytotoxic effects in the brine shrimp bioassy, with mortality rates of 73.6% and 68.9%, respectively, at a concentration of 10 μg.mL−1, and they were first isolated from endophytic fungi.

scholarly journals A Novel Approach to Derive the Predicted No-Effect Concentration (PNEC) of Benzophenone-3 (BP-3) Using the Species Sensitivity Distribution (SSD) Method: Suggestion of a New PNEC Value for BP-3

2021 ◽  
Vol 18 (7) ◽  
pp. 3650
Author(s):  
Jae-Woong Jung ◽  
Jae Soon Kang ◽  
Jinsoo Choi ◽  
June-Woo Park
Keyword(s):  
Risk Assessment ◽  
Ecological Risk ◽  
Ecological Risk Assessment ◽  
Initial Step ◽  
Species Sensitivity Distribution ◽  
Species Sensitivity ◽  
Environmental Concentration ◽  
Sensitivity Distribution ◽  
Detection Frequency ◽  
Effect Concentration

The necessity for the aquatic ecological risk assessment for benzophenone-3 (BP-3) is increasing due to its high toxic potential and high detection frequency in freshwater. The initial step in the ecological risk assessment is to determine predicted no-effect concentration (PNEC). This study derived PNEC of BP-3 in freshwater using a species sensitivity distribution (SSD) approach, whilst existing PNECs are derived using assessment factor (AF) approaches. A total of eight chronic toxicity values, obtained by toxicity testing and a literature survey, covering four taxonomic classes (fish, crustaceans, algae, and cyanobacteria) were used for PNEC derivation. Therefore, the quantity and quality of the toxicity data met the minimum requirements for PNEC derivation using an SSD approach. The PNEC derived in this study (73.3 μg/L) was far higher than the environmental concentration detected in freshwater (up to 10.4 μg/L) as well as existing PNECs (0.67~1.8 μg/L), mainly due to the difference in the PNEC derivation methodology (i.e., AF vs. SSD approach). Since the SSD approach is regarded as more reliable than the AF approach, we recommend applying the PNEC value derived in this study for the aquatic ecological risk assessment of BP-3, as the use of the existing PNEC values seems to unnecessarily overestimate the potential ecological risk of BP-3 in freshwater.

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