scholarly journals Perbedaan Pertumbuhan Artemia salina Pada Perlakuan Variasi Dosis Pakan Jus Pupa Ulat Sutra (Samia cynthia)

2020 ◽  
Vol 6 (1) ◽  
pp. 10-20
Author(s):  
Agus Dharmawan ◽  
Nurul Hikmah ◽  
Mita Larasati
Keyword(s):  
Culture Media ◽  
Average Length ◽  
Abiotic Factors ◽  
Artemia Salina ◽  
Dose Treatment ◽  
Silkworm Pupae ◽  
Silk Worm ◽  
Silkworm Pupa ◽  
Protein Feed ◽  
Observation Results

High protein feed are very necessary in the cultivation industry of Artemia salina. One of the proteins sources that can be used is silk worm pupae (Samia cynthia). The aim of this study was to determine the differences in growth of Artemia salina with the treatment of silkworm pupa juice feeding at various doses. This study uses 4 levels of treatment, namely 0.16 ml; 0.33 ml; 0.50 ml and 0.67 ml per liter with 6 replications, which are given daily for 21 days. Abiotic factors of culture media (salinity, temperature, pH, and DO) are controlled and in accordance with the needs of Artemia salina's life. Observation results on the 15th day, 17th day, and 19th day showed that there were differences in the number of Artemia salina due to the treatment of variations in the feed dose of silkworm pupae juice (Samia cynthia) with 0.67 ml feed dosage treatment having the highest average number and significantly different from other dosage treatments. Observations on the 13th day, 15th day and 21st day showed differences in the growth length of Artemia salina due to the variation of the dosage treatment of silkworm pupa juice (Samia cynthia) with 0.50 ml dose treatment having the highest average length and significantly different from other treatments. Keywords: Artemia salina, growth, feed, silkworm pupae ABSTRAK Pakan yang mengandung protein tinggi sangat diperlukan dalam budidaya Artemia salina. Salah satu sumber protein yang dapat digunakan adalah pupa ulat sutra (Samia cynthia). Tujuan penelitian ini untuk mengetahui perbedaan pertumbuhan Artemia salina dengan perlakuan pemberian pakan jus pupa ulat sutra pada berbagai dosis. Penelitian ini menggunakan 4 taraf perlakuan yaitu dosis 0,16 ml; 0,33 ml; 0,50 ml dan 0,67 ml per liter dengan 6 ulangan, yang diberikan setiap hari selama 21 hari. Faktor abiotik media kultur (salinitas, suhu, pH, dan DO) terkontrol dan sesuai dengan kebutuhan hidup Artemia salina. Hasil pengamatan hari ke-15, hari ke-17, dan hari ke-19 menunjukkan adanya perbedaan jumlah Artemia salina akibat pemberian perlakuan variasi dosis pakan jus pupa ulat sutra (Samia chyntia) dengan perlakuan dosisi pakan 0,67 ml memiliki retata jumlah tertinggi dan berbeda nyata dengan perlakuan dosis lainnya. Hasil pengamatan pada hari ke-13, hari ke-15 dan hari ke-21 menunjukkan adanya perbedaan pertumbuhan panjang Artemia salina akibat pemberian perlakuan variasi dosis pakan jus pupa ulat sutra (Samia chyntia) dengan perlakuan dosis 0,50 ml memiliki rerata panjang tertinggi dan berbeda nyata dengan perlakuan yang lain. Kata kunci: Artemia salina, pertumbuhan, pakan, pupa ulat sutra

scholarly journals Variation of Vitellaria paradoxa phenophases along the north-south gradient in Mali

1970 ◽  
pp. 08-16
Author(s):  
Bokary Allaye Kelly ◽  
Mahesh Poudyal ◽  
Jean-Marc Bouvet
Keyword(s):  
Average Length ◽  
Abiotic Factors ◽  
Permanent Plots ◽  
Future Research ◽  
Vitellaria Paradoxa ◽  
The South ◽  
The North ◽  
Study Sites ◽  
Shea Tree ◽  
Research Challenge

We monitored flowering, fruiting and leafing of Vitellaria paradoxa (shea tree) along the north–south gradient in Mali (West Africa), using three study sites for a period of three years. In each site, adult shea trees were marked and monitored in permanent plots of both field and fallow stands. The chronology of phenophases and their mean length as well as flowering and fruiting were assessed. Our data revealed significant variation according to site and stand. The onset of events starts earlier in the south than in the centre or north, but the period covered by events was almost the same for all sites (3 to 6 months for flowering; 5 to 6 months for fruiting; and 2 to 4 months before full leafing).Flowering and fruiting were more regular in the south, but often as high in the north, with an almost similar trend in both fields and fallows. In the centre, flowering was also high in fields as well as in fallows, while the fruiting was medium to high. We also observed variations in the mean length of phenological events in study sites and stands. Sites in the south showed the highest average length of flowering and leafing (76 days and 44 days, respectively), while the central site showed the greatest length of mean fruiting (110 days).  We observed a significant site*stand interaction and noticeable variation over the years. Our study indicates that phenological events of shea tree could be influenced by several interacting biotic and abiotic factors. A future research challenge in shea phenology would be to discriminate these factors and thus help sustainable management of shea tree parklands.

Use of silkworm pupae meal as protein supplement in the nutrition of broiler chickens

2005 ◽  
Vol 2005 ◽  
pp. 160-160
Author(s):  
Z. Ansari Pirsaraii ◽  
B. Navidshad
Keyword(s):  
Broiler Chickens ◽  
Crude Protein ◽  
Fish Meal ◽  
Silk Industry ◽  
Silkworm Pupae ◽  
Local Product ◽  
Chemical Score ◽  
Silk Worm ◽  
Limiting Amino Acid ◽  
Different Levels

Silkworm pupae meal is a silk industry by-product that in some countries is available as a local product. It contain up to 30% crude fat and 50% crude protein. The chemical score of silkworm pupae protein is 60 and tryptophan is its limiting amino acid. The silk worm pupae meal fat contains 25.7% linoleic acid (Udayasekhara Rao, 1994). Reddy et al (1991) reported that use of diets contain 5% 8% SWP so that replaced 50% of fish meal, lowered weight gain. The aim of this study was to measure the effects of substituting different levels of silkworm pupae (SWP) for soybean meal (SBM) in Arian (an Iranian strain) broiler rations.

scholarly journals Antibacterial, cytotoxic and trypanocidal activities of marine-derived fungi isolated from Philippine macroalgae and seagrasses

2018 ◽  
Vol 77 (2) ◽  
pp. 141-151 ◽  
Author(s):  
Kin Israel Notarte ◽  
Takashi Yaguchi ◽  
Keisuke Suganuma ◽  
Thomas Edison dela Cruz
Keyword(s):  
Gene Sequence ◽  
Culture Media ◽  
Artemia Salina ◽  
Malt Extract ◽  
Zone Of Inhibition ◽  
Potato Dextrose Broth ◽  
Gene Sequence Analysis ◽  
Marine Salt ◽  
Mycelia Sterilia ◽  
Selection Of

AbstractThe occurrence and bioactivities of marine-derived fungi are evaluated in this paper. A total of 16 morphospecies of marine-derived fungi (MDF) were isolated from four host macroalgae and two seagrasses and identified as belonging to the generaAspergillus,Fusarium,Paecilomyces,Penicillium,Sclerotinia,ThamnidiumandTrichoderma, including fivemycelia sterilia.Among these host organisms, the rhodophyteLaurencia intermediaharboured the highest number of isolated MDF. Selected MDF were then assayed and showed to inhibitPseudomonas aeruginosa(8-19 mm zone of inhibition) andStaphylococcus aureus(6-19 mm zone of inhibition), and were cytotoxic against the brine shrimpArtemia salinanauplii (LD50: 201.56-948.37 μg mL−1). The screening led to the selection of five of the most bioactive morphospecies, all belonging to the genusAspergillus. These marine aspergilli were subjected toβ-tubulingene sequence analysis for species identification, and to mass production in different culture media with or without marine salts, and screening of the crude culture extracts for their cytotoxic and trypanocidal activities.Aspergillus tubingensiscultivated in potato dextrose broth with marine salt proved to be the most cytotoxic against P388 (IC50: 1028 ng mL−1) and HeLa (IC50: 1301 ng mL−1) cancer cells. On the other hand,A. fumigatuscultivated in malt extract broth without marine salt was shown to be the most potent againstTrypanosoma congolense(IC50: 298.18 ng mL−1). Our study therefore showed that salinity may influence the bioactivities of some species of MDF.

scholarly journals Nanosilver toxicity thresholds estimative for aquatic and sediment environmental safety

2021 ◽  
Author(s):  
Vera Lucia Castro ◽  
Claudio Jonsson ◽  
Mariana Silveira Guerra M. Silva ◽  
Rodrigo Castanha ◽  
Jose Henrique Vallim ◽  
...  
Keyword(s):  
Culture Media ◽  
Aquatic Insect ◽  
Environmental Safety ◽  
Artemia Salina ◽  
Logistic Function ◽  
Environmental Concentration ◽  
Management Measures ◽  
Effect Concentration ◽  
Aquatic Compartment ◽  
Expected Increase

Abstract Silver nanoparticles (AgNPs) have been largely utilized. Despite that, comprehensive studies on their ecotoxicological effects and environmental risk are still required. In the present study, the AgNPs effect was assessed to some organisms including algae, plants, microcrustaceans, cnidaria, nematodes, aquatic insect, earthworm, and fish embryos. The no-observed-effect concentration (NOEC) was calculated for each organism using a log-logistic function. AgNPs remained stable in contact with culture media during the analyzed period and conditions employed, presenting dispersion less than 20%, except for Artemia salina medium. On this occasion, NPs presented dispersion of approximately 25%, although their size remained unchangeable. The Predicted No Effect Concentration (PNEC) of AgNPs in the aquatic compartment was estimated in the concentration range from 0.13 to 0.66 µg L-1. A Risk Quotient (RQ) of 15.1 was derived for the NP tested with the aid of a maximum AgNPs Predicted Environmental Concentration (PEC) estimated value. The RQ value superior to one indicates a risk and the need for its management measures implementation. These data, in addition to the expected increase in AgNPs use, reinforce the importance of the AgNPs safety levels establishment that can contribute to performing their risk assessment studies.

scholarly journals Organic fertilizer on the in vitro cultivation of the Cattleya labiata orchid

2017 ◽  
Vol 2 (2) ◽  
pp. 62
Author(s):  
Rodrigo Thibes Hoshino ◽  
Ronan Carlos Colombo ◽  
Ana Paula Zandoná ◽  
Guilherme Augusto Cito Alves ◽  
Ricardo Tadeu de Faria
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Average Length ◽  
Organic Fertilizer ◽  
Dry Mass ◽  
In Vitro Cultivation ◽  
Shoot Height ◽  
Organic Products ◽  
Number Of Leaves

Propagation of in vitro plants through other culture media rather than the traditional ones has been widely researched, with satisfactory results. However, to increase the effectiveness of these media, the addition of organic products has been presented satisfactory results. Therefore, the objective of this work was to evaluate the influence of the FishFértil® organic fertilizer on the in vitro cultivation of the Brazilian orchid Cattleya labiata. Treatments included FishFértil® fertilizer concentrations of 0; 1; 2; 3; 4; 5 and 6 mL L-1 in a simplified culture medium. At 180 days, shoot height, number of leaves, leaf area, number of roots, root average length, shoot and root dry mass and the shoot: root ratio were evaluated. The experimental design was entirely randomized, with 10 replications, each one containing 10 plantules. Data were submitted to an analysis of variance and regression analysis, at 5% of significance. The FishFértil® organic fertilizer at the concentration of 6 mL L-1 promoted better plantule growth of Cattleya labiata, subcultivated in vitro, in a simplified culture medium.

scholarly journals ​In-vitro Effect of Different Abiotic Stresses on the Growth and Sporulation of Colletotrichum gloeosporioides causing Anthracnose of Mango

2021 ◽  
Author(s):  
R. Kannan ◽  
V. Dhivya
Keyword(s):  
Fungal Pathogen ◽  
Radial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Inhibitory Concentration ◽  
Culture Media ◽  
Abiotic Factors ◽  
Potato Dextrose Agar ◽  
Oatmeal Agar ◽  
Vitro Effect

Background: Mango productivity was very much affected due to a major fungal pathogen, Colletotrichum gloeosporioides causing anthracnose mango rot. The present study was carried out to investigate the influence of abiotic factors for the support of superficial growth of isolated fungus and finding a minimum inhibitory concentration of different fungicides. Methods: Among four different culture media tested, the highest radial growth and sporulation of the fungus were recorded in Oatmeal agar (OMA) (84 mm) followed by Conn’s agar (CA), Czapek Dox agar (CDA) and Potato dextrose agar (PDA). Among the different pH tested, pH 7.0 was found to be the best in supporting the good radial growth (69 mm) followed by pH 6.0 (56 mm), pH 5.5 (49 mm), pH 7.5 (43 mm) and pH 8.0 (37 mm). Among the various temperature tested, 25oC (69.32) was found to be the best followed by 20oC (52.53 mm), 30oC (65.23 mm) and 35oC. Result: Among the fungicides tested, Zineb 68% + Hexaconazole 4% WP (avtar) was found best as the radial growth was observed to be 45, 41, 36, 32, 25 mm at 5, 10, 25, 50 and 100 ppm, respectively as compared to 80 mm in control. The fungicide Tricyclazole 18% + Mancozeb 62% WP (Merger) was found to be the least effective in checking the radial growth of C. gloeosporioides even at 100 ppm concentration.

Nutritional targeting modification of silkworm pupae oil catalyzed by a smart hydrogel immobilized lipase

2021 ◽  
Author(s):  
Jin-Zheng Wang ◽  
Cheng-Kun Wu ◽  
Cheng-Hai Yan ◽  
Huan Chen ◽  
Shuai You ◽  
...  
Keyword(s):  
Immobilized Lipase ◽  
Nutritional Supplement ◽  
Relative Content ◽  
Smart Hydrogel ◽  
Microfluidic Reactor ◽  
Silkworm Pupae ◽  
Silkworm Pupa

To prepare a nutritional supplement using silkworm pupa oil (SPO) as a feedstock, a microfluidic reactor with smart hydrogel immobilized lipase was firstly constructed to reduce the relative content of...

Ratio of C:N in culture media of silk worm, Tubifex sp.

2014 ◽  
Vol 2 (1) ◽  
pp. 19
Author(s):  
Jhonly Solang ◽  
Henneke Pangkey ◽  
Stenly Wullur ◽  
Sartje Lantu
Keyword(s):  
Culture Media ◽  
C:N Ratio ◽  
Chicken Manure ◽  
Pig Manure ◽  
Running Water ◽  
Manure Treatment ◽  
Soybean Curd ◽  
Silk Worm ◽  
Soybean Curd Residue ◽  
And Control

Title (Bahasa Indonesia): Rasio C:N pada media kultur cacing sutra, Tubifex sp. This study aimed to determine the C:N ratio on each medium for the growth of the sludge worm. This study used mud and soybean curd residue (treatment A), mud and chicken manure (treatment B), mud and pig manure (treatment C), and control (mud only) (K) as culture media of the sludge worm (Tubifex sp.). The decomposition process was proceeding for six days, and then the sludge worms were stocked with initial weight of 30 grams per container. Culture period was 21 days in running water systems. The resulting C:N ratio was 60.5 for treatment A, 45.8 for treatment B, 36 for treatment C and 35 for K. The soybean curd residue and mud medium gave the highest influence on the growth of the sludge worm, followed by pig manure and mud, chicken manure and mud, and then mud (control). Penelitian ini bertujuan untuk menentukan perbandingan C:N ratio dalam media budidaya untuk pertumbuhan cacing sutra. Penelitian ini menggunakan lumpur dan ampas tahu (perlakuan A), lumpur dan kotoran ayam (perlakuan B), serta lumpur dan kotoran babi (perlakuan C) dan perlakuan K (kontrol: hanya lumpur) sebagai media kultur cacing sutra (Tubifex sp). Proses dekomposisi dilakukan selama 6 hari, kemudian dilakukan penebaran cacing sutra dengan berat awal 30 gram/wadah penelitian. Waktu pemeliharaan dilakukan selama 21 hari dalam sistem air mengalir. Hasil penelitian menunjukkan rasio C:N sebesar 60,55 untuk perlakuan A, 45,85 untuk perlakuan B, 36,08 untuk perlakuan C, dan 35,25 untuk K. Media yang menggunakan ampas tahu dan lumpur memberikan pengaruh tertinggi terhadap pertumbuhan cacing sutra, kemudian disusul oleh media kotoran babi dan lumpur, media kotoran ayam dan lumpur dan terendah media lumpur (kontrol).

scholarly journals Cytotoxic Metabolites Produced by Alternaria No.28, an Endophytic Fungus Isolated from Ginkgo biloba

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Jian-Chun Qin ◽  
Ya-Mei Zhang ◽  
Ling Hu ◽  
Ya-Tuan Ma ◽  
Jin-Ming Gao
Keyword(s):  
Ginkgo Biloba ◽  
Endophytic Fungus ◽  
Brine Shrimp ◽  
Culture Media ◽  
Monomethyl Ether ◽  
Artemia Salina ◽  
Fungal Endophyte ◽  
Alternariol Monomethyl Ether ◽  
Cytotoxic Effects

From the medicinal plant Ginkgo biloba the fungal endophyte Alternaria no.28 was isolated. Extract of the fungus grown in liquid culture media exhibited marked cytotoxic activity when tested in vitro against brine shrimp (Artemia salina). Eight compounds were isolated from the extract of cultures of this endophytic fungus and were elucidated as alterperylenol (1), altertoxin I (2), alternariol (3), alternariol monomethyl ether (4), tenuazonic acid (5) and its derivative (6), together with ergosterol and ergosta-4, 6, 8, 22-tetraen-3-one by means of spectroscopic analysis. Among them, both 5 and 6 showed significant cytotoxic effects in the brine shrimp bioassy, with mortality rates of 73.6% and 68.9%, respectively, at a concentration of 10 μg.mL−1, and they were first isolated from endophytic fungi.

Sign in / Sign up

Export Citation Format

Share Document