scholarly journals Serum Samples Cannot Be Used for Fluorescence Immunoassay for detection of myocardial infarction triple

2020 ◽  
Author(s):  
Wanju Xu ◽  
Zhonghua Gong ◽  
Yan Yan ◽  
Shiyu Lv ◽  
Jiazheng Wang
Keyword(s):  
Myocardial Infarction ◽  
Whole Blood ◽  
False Negative ◽  
Immunofluorescence Assay ◽  
Assay Method ◽  
Serum Samples ◽  
Blood Samples ◽  
Negative Results ◽  
Positive Rate ◽  
Whole Blood Samples

Abstract Background: Whole blood or plasma samples are recommended for use in triple-marker testing of myocardial infarction. Whether serum sample can be used for the diagnosis of myocardial infarction has not been compared and validated.Methods: Whole blood samples and serum samples were detected with CTnI, Mb and CK-MB simultaneously by using immunofluorescence assay method.Results: Both CK-MB and TNI detection results were highly consistent for whole blood samples vs. serum samples. However, when Mb is tested, the positive rate of serum samples is relatively low, and there will be more false negative results, resulting in missed diagnosis.Conlusion: Serum samples cannot be used in replace of whole blood samples for triple-marker testing and diagnosis of myocardial infarction.

scholarly journals Quantifying Serum Antiplague Antibody with a Fiber-Optic Biosensor

1998 ◽  
Vol 5 (5) ◽  
pp. 609-612 ◽  
Author(s):  
George P. Anderson ◽  
Keeley D. King ◽  
Lynn K. Cao ◽  
Meagan Jacoby ◽  
Frances S. Ligler ◽  
...  
Keyword(s):  
Fiber Optic ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Cells ◽  
Serum Samples ◽  
Blood Samples ◽  
Competitive Assay ◽  
Protein Toxins ◽  
Whole Blood Samples ◽  
Good Agreement

ABSTRACT The fiber-optic biosensor, originally developed to detect hazardous biological agents such as protein toxins or bacterial cells, has been utilized to quantify the concentration of serum antiplague antibodies. This biosensor has been used to detect and quantify the plague fraction 1 antigen in serum, plasma, and whole-blood samples, but its ability to quantify serum antibodies has not been demonstrated. By using a competitive assay, the concentration of serum antiplague antibodies was ascertained in the range of 2 to 15 μg/ml. By making simple dilutions, concentrations for 11 serum samples whose antiplague antibody concentrations were unknown were determined and were found to be in good agreement with enzyme-linked immunosorbent assay results. The competitive assay method could be used to effectively determine the exposure to plague of animals or humans or could be applied to other diseases, such as hepatitis or AIDS, where the presence of antibodies is used to diagnose infection.

Washing-free Chemiluminescence Immunoassay for Rapid Detection of cTnI in Whole Blood Samples

2021 ◽  
Author(s):  
Huan Zhao ◽  
Enben Su ◽  
Li Huang ◽  
Yunfeng Zai ◽  
Yuan Liu ◽  
...  
Keyword(s):  
Whole Blood ◽  
Rapid Detection ◽  
Magnetic Particles ◽  
Troponin I ◽  
Sample Type ◽  
Serum Samples ◽  
Chemiluminescence Immunoassay ◽  
Blood Samples ◽  
Significant Difference ◽  
Whole Blood Samples

Abstract Background: Chemiluminescence immunoassay (CLIA) has always been a great challenge in detecting whole blood samples without centrifugation because of the interference of red blood cells and low sensitivity. Results: In this scheme, the antigens and erythrocytes in the blood were captured by the antibodies immobilized on the magnetic particles, recognized by another biotin-conjugated cTnI antibody and detected by streptavidin/acridine aster-conjugated PCMS. After magnetic separation, the supernatant was transferred and measured. No significant difference was noted between the cTnI concentrations of the serum samples, plasma samples and whole blood. The prepared PCMS provided more functional areas to conjugate streptavidin and acridinium ester, so the immunoassay has highly sensitive, the limits of blank at 0.012 ng/mL, and functional sensitivity at 0.019 ng/mL with a CV of 20%, and 0.058 ng/mL with a CV of 10%. Total precision of any sample type ranged from 2.62~5.67%. The assay was linear over the studied range of 0.01–50.00 ng/mL, and no hook effect was found when cTnI concentrations reached 1900 ng/mL. No significant interference was noted with the potential endogenous interfering substances. Compared with the commercial kit (Abbott assay kit), the correlation coefficient was 0.9859.Conclusions: A washing-free chemiluminescence immunoassay (CLIA) was established for the rapid detection of cardiac troponin I (cTnI) in human whole blood, using erythrocyte capture antibodies-conjugated magnetic nanoparticles for eliminating the influence of erythrocytes and polychloromethylstyrene microspheres (PCMS) for signal amplification, which showed great potential in clinical application.

Aqueous and serum-based materials compared for use as simulated calibrators for three ionized calcium analyzers.

1986 ◽  
Vol 32 (8) ◽  
pp. 1548-1550 ◽  
Author(s):  
J Toffaletti ◽  
C Bird ◽  
C Berg ◽  
B Abrams
Keyword(s):  
Reference Material ◽  
Human Serum ◽  
Whole Blood ◽  
Calcium Ion ◽  
Ionized Calcium ◽  
Serum Samples ◽  
Blood Samples ◽  
Serum Pool ◽  
Accuracy And Precision ◽  
Whole Blood Samples

Abstract To determine if bias between different ionized calcium analyzers could be decreased, we analyzed 10 control fluids during a study in which ionized calcium was measured in more than 150 serum and whole-blood samples. After calibrating three ionized calcium analyzers (Radiometer ICA 1, Nova 8, and AVL 980) with the manufacturers' respective calibrators, we used the between-instrument differences of the control fluids to simulate recalibration of the analyzers during each analytical run. A filtered human serum pool containing ionized calcium at 1 mmol/L concentration, with CO2 removed and having no added buffer, was the only material that consistently decreased between-analyzer bias of both serum and whole blood. Another human serum pool containing about 1.3 mmol of ionized calcium and about 10 mmol of bicarbonate per liter was even better at minimizing analyzer biases for serum samples, but was not as effective for whole-blood samples. Some additives used to buffer pH apparently adversely affected both the accuracy and precision of some, but not other, calcium ion electrodes. We conclude that if a reference material is developed for calibration of ionized calcium analyzers, it should be tested on several analyzers for use with both serum and whole blood, and it should be at least as effective as a human serum material, such as that used here.

scholarly journals Rapid, Sensitive, and Specific Lateral-Flow Immunochromatographic Point-of-Care Device for Detection of Herpes Simplex Virus Type 2-Specific Immunoglobulin G Antibodies in Serum and Whole Blood

2007 ◽  
Vol 15 (1) ◽  
pp. 159-163 ◽  
Author(s):  
Elisabeth I. Laderman ◽  
Emma Whitworth ◽  
Erickson Dumaual ◽  
Mark Jones ◽  
Andrew Hudak ◽  
...  
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Cross Reactivity ◽  
Serum Samples ◽  
Blood Samples ◽  
Positive Serum ◽  
Whole Blood Samples ◽  
Simplex Virus ◽  
Sensitivity Specificity

ABSTRACT Herpes simplex virus type 2 (HSV-2) is a common human pathogen that can cause a variety of clinical manifestations in humans. In order to provide near-patient results to allow for faster counseling and treatment, a rapid point-of-care test that is accurate and simple to use is desirable. Here, we describe the development and evaluation of an HSV-2 immunoglobulin G (IgG)-specific antibody lateral-flow immunochromatographic assay (LFIA) based on colloidal gold nanoparticles. A total of 359 serum samples and 100 whole-blood samples were tested in the newly developed HSV-2 LFIA. Serum results were compared to those from the HerpeSelect HSV-2 enzyme-linked immunosorbent assay (ELISA), and whole-blood sample results were compared to those of both ELISA and HerpeSelect HSV-1 and -2 immunoblotting (IB). The sensitivity of the HSV-2 LFIA compared to that of the HerpeSelect ELISA was 100% (89/89), and the specificity was 97.3% (257/264). Cross-reactivity with HSV-1 IgG-positive serum samples was observed in 2.6% (5/196) of samples, 2.9% (1/34) for rubella virus, and 6.2% (1/16) for Epstein-Barr virus. No cross-reactivity in varicella-zoster virus or cytomegalovirus IgG-positive serum samples was observed. No interference was observed from bilirubin-, triglyceride-, albumin-, or hemoglobin-spiked samples. The concordance of the LFIA results between capillary whole blood, EDTA-treated venous whole blood, heparin-treated venous whole blood, and serum was 99% (99/100). In conclusion, the LFIA for HSV-2 IgG-specific antibodies demonstrated excellent sensitivity, specificity, and concordance for both serum and whole-blood samples compared to the sensitivity, specificity, and concordance of both HSV-2 ELISA and IB.

Steadiness/stability of antiepileptic drugs in serum, plasma and whole-blood samples under various storage methods

2010 ◽  
Vol 41 (02) ◽  
Author(s):  
N Shazi ◽  
A Böss ◽  
HJ Merkel ◽  
F Scharbert ◽  
D Hannak ◽  
...  
Keyword(s):  
Antiepileptic Drugs ◽  
Whole Blood ◽  
Blood Samples ◽  
Storage Methods ◽  
Whole Blood Samples

scholarly journals Determination of 19 Psychoactive Substances in Premortem and Postmortem Whole Blood Samples Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Separations ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 78
Author(s):  
Sevasti Karampela ◽  
Jessica Smith ◽  
Irene Panderi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
Whole Blood ◽  
High Performance ◽  
Tandem Mass ◽  
Psychoactive Substances ◽  
Blood Samples ◽  
Whole Blood Samples

An ever-increasing need exists within the forensic laboratories to develop analytical processes for the qualitative and quantitative determination of a broad spectrum of new psychoactive substances. Phenylethylamine derivatives are among the major classes of psychoactive substances available on the global market and include both amphetamine analogues and synthetic cathinones. In this work, an ultra-high-performance liquid chromatography-positive ion electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) has been developed and fully validated for the determination of 19 psychoactive substances, including nine amphetamine-type stimulants and 10 synthetic cathinone derivatives, in premortem and postmortem whole blood. The assay was based on the use of 1 mL premortem or postmortem whole blood, following solid phase extraction prior to the analysis. The separation was achieved on a Poroshell 120 EC-C18 analytical column with a gradient mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water in 9 min. The dynamic multiple reaction monitoring used in this work allowed for limit of detection (LOD) and lower limit of quantitation (LOQ) values of 0.5 and 2 ng mL−1, respectively, for all analytes both in premortem and postmortem whole blood samples. A quadratic calibration model was used for the 12 quantitative analytes over the concentration range of 20–2000 ng mL−1, and the method was shown to be precise and accurate both in premortem and postmortem whole blood. The method was applied to the analysis of real cases and proved to be a valuable tool in forensic and clinical toxicology.

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