scholarly journals Assembly of Two New Coordination Polymers: Luminescent Properties and Anti-inflammatory Activity on Postoperative Infectious Endophthalmitis

2021 ◽  
Author(s):  
Guang-Qing Bian ◽  
Shi-Xi Zhang ◽  
Xiao-Li Qu
Keyword(s):  
Room Temperature ◽  
Luminescent Properties ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Specific Mechanism ◽  
Pathway Activation ◽  
Intense Luminescence ◽  
Metal Organic ◽  
Polymerase Chain ◽  
Infectious Endophthalmitis

Abstract The solvothermal reactions via the self-assemble approach produced two new metal-organic compounds, i.e, [Zn(4-cptpy)(HCOO)(H2O)]n·n(DMF) (1) and [Cd3(btc)2(4-cptpyH)(DMF)(H2O)3]n (2) (DMF is N,N’-dimethylformamide, H3btc is benzene-1,3,5-tricarboxylic acid, and 4-Hcptpy is 4-[2,6-bis(pyridine-4-yl)pyridine-4-yl]benzoic acid). Furthermore, the compounds’ luminescent performances have also been studied. As a result, compounds 1-2 show intense luminescence at room temperature. The evaluation of their application values against postoperative infectious endophthalmitis (PIE) was implemented and their specific mechanism was investigated. First of all, the real time reverse transcription-polymerase chain reaction (RT-PCR) was performed and the nuclear factor kappa-B (NF-κb) signaling pathway activation levels were measured. In addition to this, the inflammatory cytokines content released after the cataract surgery was determined through exploiting the enzyme linked immunosorbent assay (ELISA) detection kit.

scholarly journals Evaluation of Sandwich Enzyme-Linked Immunosorbent Assay and Reverse Transcription Polymerase Chain Reaction for the Diagnosis of Foot-and-Mouth Disease

Intervirology ◽  
2021 ◽  
pp. 1-6
Author(s):  
Salman Khan ◽  
Syed Asad Ali Shah ◽  
Syed Muhammad Jamal
Keyword(s):  
Polymerase Chain Reaction ◽  
Viral Genome ◽  
Foot And Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Kappa Value ◽  
Polymerase Chain ◽  
Pcr Assays ◽  
Level Of Agreement

<b><i>Background:</i></b> Foot-and-mouth disease (FMD) is an infectious and highly contagious disease of cloven-hoofed domestic and wild animals, causing heavy economic losses to the livestock industry. Rapid and reliable diagnosis of the disease is essential for the implementation of effective control measures. This study compared sandwich enzyme-linked immunosorbent assay (S-ELISA) and conventional reverse transcription polymerase chain reaction (RT-PCR) for the diagnosis of FMD. <b><i>Methods:</i></b> A total of 60 epithelial samples from suspected cases of FMD were tested using both S-ELISA and RT-PCR assays. The level of agreement between the assays was assessed by calculating the Kappa value. <b><i>Results:</i></b> S-ELISA detected 38 (63%) samples positive for FMD virus (FMDV). Being predominant, serotype O was detected in 22 (57.9%) of the total samples tested positive, whereas 9 (23.7%) and 7 (18.4%) samples were found positive for serotypes A and Asia-1, respectively. RT-PCR detected viral genome in 51 (85%) of the samples using pan-FMDV primers set, 1F/1R. Thirty-six samples were found positive and 7 negative by both the tests. The level of agreement between the tests was assessed by calculating the Kappa value, which was found to be fair (Kappa value = 0.303 and 95% CI = 0.089; 0.517) and significant (<i>p</i> = 0.009). However, 2 samples, which were found positive on S-ELISA tested negative on RT-PCR. This may be attributed to the presence of nucleotide mismatch(es) in the primer-binding sites that may have resulted in failure of amplification of the viral genome. The serotype-specific RT-PCR assays not only confirmed serotyping results of S-ELISA but were also able to establish serotype in 9 S-ELISA-negative but pan-FMDV RT-PCR-positive samples. <b><i>Conclusions:</i></b> The RT-PCR assay contributes significantly to establishing a quick, sensitive, and definitive diagnosis of FMD in resource-constrained countries. Samples giving negative results in S-ELISA should be tested in RT-PCR for the disease detection and virus typing.

scholarly journals Monitoring Current-Season Spread of Potato virus Y in Potato Fields Using ELISA and Real-Time RT-PCR

Plant Disease ◽  
2013 ◽  
Vol 97 (5) ◽  
pp. 641-644 ◽  
Author(s):  
Manphool S. Fageria ◽  
Mathuresh Singh ◽  
Upeksha Nanayakkara ◽  
Yvan Pelletier ◽  
Xianzhou Nie ◽  
...  
Keyword(s):  
Real Time ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Current Season ◽  
Seed Market ◽  
Polymerase Chain ◽  
Time Of Harvest

The current-season spread of Potato virus Y (PVY) was investigated in New Brunswick, Canada, in 11 potato fields planted with six different cultivars in 2009 and 2010. In all, 100 plants selected from each field were monitored for current-season PVY infections using enzyme-linked immunosorbent assay (ELISA) and real-time reverse-transcription polymerase chain reaction (RT-PCR) assay. Average PVY incidence in fields increased from 0.6% in 2009 and 2% in 2010 in the leaves to 20.3% in 2009 and 21.9% in 2010 in the tubers at the time of harvest. In individual fields, PVY incidence in tubers reached as high as 37% in 2009 and 39% in 2010 at the time of harvest. Real-time RT-PCR assay detected more samples with PVY from leaves than did ELISA. A higher number of positive samples was also detected with real-time RT-PCR from growing tubers compared with the leaves collected from the same plant at the same sampling time. PVY incidence determined from the growing tubers showed a significant positive correlation with the PVY incidence of tubers after harvest. Preharvest testing provides another option to growers to either top-kill the crop immediately to secure the seed market when the PVY incidence is low or leave the tubers to develop further for table or processing purposes when incidence of PVY is high.

scholarly journals Epidemiology and Control of Congo Fever in Sacrificial Animals of Pakistan

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Hafiz Muhammad Rizwan ◽  
Muhammad Sohail Sajid ◽  
Haider Abbas ◽  
Muhammad Fiaz Qamar ◽  
Qaiser Akram
Keyword(s):  
Tick Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Many Body ◽  
Rt Pcr ◽  
Body Tissues ◽  
Crimean Congo Haemorrhagic Fever ◽  
Polymerase Chain ◽  
And Control ◽  
Successful Prevention ◽  
Venereal Transmission

The cases and deaths due to Crimean-Congo haemorrhagic fever (CCHF) [49] virus commonly known as Congo virus (fatality rate 15%) have been reported throughout Pakistan from the last five years especially during religious occasion, Eid-ul-Azha. The annual increase in death rates due to CCHF demonstrate the importance of awareness of Congo fever at academia as well as public level. The symptoms of Congo fever which appear one to nine days after tick bite, include sudden high fever, muscle aches, abdominal pain, headache, dizziness, sore eyes, jaundice, mood swings, confusion, aggression, and sensitivity to light. The other signs include sore throat, joint pain, vomiting, diarrhea, hemorrhages, and bleeding from skin and large intestine. The Infection has been reported in many species of wild as well as domestic animals including hares, cattle, sheep, goats, dogs, mice and hedgehogs. At least 31 species of Hyalomma, Boophilus, Rhipicephalus, Dermacentor (Ixodidae: hard ticks) act as vector of CCHF in which transovarial, transstadial and venereal transmission occurs. The virus attacks the immune system of the host and influences the immune cells. The Congo fever virus can be isolated from blood, plasma and many body tissues (kidneys, liver, spleen, lungs, brain and bone marrow). Mice inoculation, enzyme-linked immunosorbent assay (ELISA), reverse transcription polymerase chain reaction (RT-PCR) can be used for detection of the infection. Furthermore, IgM and IgG antibodies against CCHFV can also be detected and quantified. Education of general public, tick control with acaricides, use of anti-CCHFV immunoglobulin, usage of approved repellents to prevent tick bites, wearing neutral-coloured garments, application of a permethrin spray to the clothing, avoiding tall grasses and shrubs, applying sunscreen, avoiding direct contact with the blood or tissues of animals are the factors for successful prevention of the infection.

scholarly journals Identification et caractérisation des isolats d'orbivirus des îles de la Réunion et de la Martinique

2009 ◽  
Vol 62 (2-4) ◽  
pp. 149
Author(s):  
D. Vitour ◽  
Corinne Sailleau ◽  
Emmanuel Breard ◽  
Stéphan Zientara
Keyword(s):  
Sequence Analysis ◽  
Clinical Symptoms ◽  
Enzyme Linked Immunosorbent Assay ◽  
La Réunion ◽  
Rt Pcr ◽  
Polymerase Chain ◽  
The One ◽  
Almost All ◽  
Haemorrhagic Disease

At the beginning of 2009, bluetongue (BT)-like clinical symptoms were reported in cattle on the French island of La Réunion (Indian Ocean). One hundred and twenty-three cows were blood tested for the presence of BT and/or epizootic haemorrhagic disease virus (EHDV) ribonucleic acid (RNA) by group specific reverse transcriptase polymerase chain reaction (RT-PCR). EHDV RNA was detected in 111 samples (90.2%), among which five were also positive for BTV RNA. Sequence analysis of EHDV segment 7 revealed that this circulating strain seemed to be similar to the one isolated in 2003 (99.8% nucleotide identity). The determination of the nucleotide sequence of segment 2 is under investigation. The vironeutralization test (VNT), serotype-specific RT-PCR, as well as sequence analysis identified the isolated BTV strain as serotype 2. These data showed that an EHDV outbreak occurred over the last winter in La Réunion, and it was concomitant to circulation of BTV. Epidemic or enzootic features of both these viruses are not yet known. Since this outbreak, molecular and serological tools specific to EHDV have been or are being developed. Three years ago, 30 healthy head of cattle moved from Metropolitan France to the French Martinique Island (Caribbean Basin) and were distributed in four different farms. Animals were sampled (blood and serum) every 10 days until day 30 and tested for BTV infection by the enzyme-linked immunosorbent assay (ELISA) or RT-PCR assays. Unexpectedly, almost all animals became BTV positive within 20 days. Whenever possible, virus isolation on eggs and baby hamster kidney (BHK) cell cultures were performed. Interestingly, seven BT strains belonging to seven distinct serotypes (BTV-2, 9, 10, 17, 18, 22, 24) were isolated. The coding sequence of segments 7, 8, 9 and 10 of these seven serotypes was obtained, as well as a portion of segment 2. The phylogenetic analysis revealed an unprecedented divergence of these strains with other known BTV sequences.

scholarly journals Serological and Molecular Detection of Hepatitis B virus among patients referred to Kurdistan Center for Hepatology and Gastroenterology in Sulaimani City/Kurdistan Region of Iraq

2020 ◽  
Vol 4 (2) ◽  
pp. 117
Author(s):  
Raz Sirwan Abdulla ◽  
Salih Ahmed Hama
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hbv Dna ◽  
Molecular Techniques ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
B Virus ◽  
Polymerase Chain ◽  
Pcr Techniques ◽  
Positive Results

Hepatitis B virus infection is caused by the hepatitis B virus, a major global health problem. This infection can lead to chronic conditions, followed by cirrhosis and hepatocellular carcinoma (HCC). The current study was aimed to detect HBV using serological and molecular techniques. During 2019, 300 blood samples were collected from Kurdistan Center for Hepatology and Gastroenterology in Sulaimani city. Enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR) techniques were used for the detection of HBsAg and HBV DNA, respectively. Obtained results were revealed that 92 out of 300 tested patients (30.66%) seropositive for HBsAg. Among 92 seropositive patients, 53 were shown positive results for HBV DNA by RT-PCR. Dental clinic visiting and dialysis were among the important risk factors for HBV transmission. The vast majority of positive results were among males. Smokers showed relatively high rates of positive results. One-third of the referred patients who had liver complaints were positive for HBsAg. More than half of the seropositive patients showed RT-PCR positive results. It was concluded that the molecular method (RT-PCR) is more sensitive and gives a more accurate result than serology (ELISA). Therefore, it can be used as a diagnostic tool for HBV detection.

scholarly journals Seeing beyond a Dilated Proventriculus: Diagnostic Tools for Proventricular Dilatation Disease in Psittacine Birds

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3558
Author(s):  
Jeann Leal de Araújo ◽  
Raquel Rubia Rech
Keyword(s):  
Molecular Techniques ◽  
Diagnostic Tools ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Postmortem Diagnosis ◽  
Concise Review ◽  
Life Threatening ◽  
Polymerase Chain ◽  
Proventricular Dilatation Disease ◽  
Diagnostic Outcomes

Proventricular dilatation disease (PDD) is a life-threatening neurological disease caused by parrot bornaviruses (PaBVs) that affects several species worldwide. PDD can be clinically manifested as either a central nervous system condition or a gastrointestinal condition if the nerves and ganglia of the gastrointestinal tract are compromised. We intend to provide a concise review for veterinary clinicians and diagnosticians with focus on the main tools available for PDD diagnosis, including gross and histopathology, immunohistochemistry, molecular techniques and serology. We suggest that a combination of different strategies can increase the success of diagnostic outcomes, as tools such as reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) can be implemented for identification of bornaviral infections in live patients, and gross pathology, histopathology, immunohistochemistry and RT-PCR can provide reliable results for postmortem diagnosis of PDD.

Detection of small round structured viruses in clinical and environmental samples by polymerase chain reaction

1995 ◽  
Vol 31 (5-6) ◽  
pp. 375-382 ◽  
Author(s):  
M. Wolfaardt ◽  
C. L. Moe ◽  
W. O. K. Grabow
Keyword(s):  
Polymerase Chain Reaction ◽  
Tap Water ◽  
Practical Method ◽  
Glass Wool ◽  
Polluted Water ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Elution Method

Norwalk (NV) and other small round structured viruses (SRSVs) have been identified as common causes of gastroenteritis. Outbreaks of Norwalk gastroenteritis have been associated with contaminated drinking water and food such as oysters and salads. The cloning and sequencing of the NV genome has made it possible to detect NV and related viruses by the reverse transcription-polymerase chain reaction (RT-PCR). We applied RT-PCR to detect SRSVs in faecal specimens from two gastroenteritis outbreaks in South Africa, designated “Christmas” and “Grootbrak” and were able to detect SRSVs in all of the three specimens from the Christmas outbreak and in two of 16 specimens from the Grootbrak outbreak. The RT-PCR procedure used appeared to be more sensitive for the detection of SRSVs in patient stool specimens than immune electron microscopy and NV antigen detection by enzyme linked immunosorbent assay. The RT-PCR procedure proved suitable for the detection of SRSVs in seeded samples of sewage, sewage sludge, river water, and tap water. However, sensitivity was lower for seeded samples of sewage and sludge than for tap water, which indicates interference by high levels of organic matter. The RT-PCR procedure was also used to show that small numbers of SRSVs can successfully be recovered from large volumes of water by means of a glass wool adsorption-elution method. Since no practical method is available for quantitation of the small numbers of SRSVs concerned, it was not possible to evaluate the efficiency of recovery. Although no SRSVs have been detected by direct testing of sewage and sludge samples, the results obtained in this study show that RT-PCR detection of SRSVs in sewage and polluted water environments is feasible, and that small numbers of the viruses can, like many other enteric viruses, successfully be recovered by means of a glass wool adsorption-elution method.

Fatal Hantavirus Pulmonary Syndrome in an Adolescent

PEDIATRICS ◽  
1995 ◽  
Vol 95 (2) ◽  
pp. 276-280 ◽  
Author(s):  
Ali S. Khan ◽  
Thomas G. Ksiazek ◽  
Sherif R. Zaki ◽  
Stuart T. Nichol ◽  
Pierre E. Rollin ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Immunoglobulin M ◽  
Hantavirus Pulmonary Syndrome ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sin Nombre Virus ◽  
Rt Pcr ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Polymerase Chain ◽  
Formalin Fixed

Hantavirus pulmonary syndrome (HPS) is a recently recognized viral zoonosis characterized by a febrile prodrome progressing to severe noncardiogenic pulmonary edema.1-4 This syndrome is caused by at least three newly described hantaviruses: the first, Sin Nombre virus, is the Southwestern hantavirus that caused an outbreak of respiratory failure during the summer of 1993; the second, Black Creek Canal virus, caused a case of HPS in Florida; and the third hantavirus was identified in lung tissue from a patient in Louisiana. Diagnosis is by enzyme-linked immunosorbent assay (ELISA) serology with elevated immunoglobulin M (IgM) titers against heterologous and homologous hantaviral antigens, positive immunohistochemistry on formalin-fixed tissue, or reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of hantaviral nucleotide sequence from frozen tissue.

Investigation of Rotavirus infection in sheep usingserological and molecular techniques

2016 ◽  
Author(s):  
Volkan Yilmaz. ◽  
M.Ozkan Timurkan ◽  
Nuvit Coskun ◽  
Yakup Yildirim
Keyword(s):  
Molecular Detection ◽  
Molecular Techniques ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Scale ◽  
Family Farms ◽  
Rt Pcr ◽  
Fecal Samples ◽  
Rotavirus Infection ◽  
Scale Family ◽  
Polymerase Chain

In this study, serological and molecular research was conducted on the Rotavirus infection in domestic breeds of sheep at 2–3 years of age. The sheep included in the study were raised on small scale family units of less than 20 sheep per unit, in central Kars province and its districts (Susuz, Arpaçay, Kagizman and Selim) in the Northeast Anatolia region of Turkey. The blood and fecal samples were collected randomly from 450 sheep. They were analyzed for the presence of Rotavirus and the antibody against the virus using enzyme-linked immunosorbent assay (ELISA). The highest seropositive ratio (73.46%) was found in central Kars province. The seroprevalence of Rotavirus in sheep raised in the Kars region was determined to be 55.33%. Rotavirus was not detected in fecal samples with ELISA. Molecular detection of Rotavirus from fecal samples was done by reverse transcription polymerase chain reaction (RT-PCR) technique using specific generic primers for VP6 protein. Rotavirus could not be detected in RT-PCR. The data that were obtained showed that the infection spreads on small scale family farms. Based on this information, recommendations were made for controlling Rotavirus infection.

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