scholarly journals Genome-Wide Study of the ABI3 Gene Family and Identification of Putative miRNA Targeting ABI3 Gene in Oryza Sativa ssp. Indica

2021 ◽  
Author(s):  
Apoorva Vashisth ◽  
Deepak Kumar Singh ◽  
Navjyoti Chakraborty ◽  
Ram Singh Purty ◽  
Sayan Chatterjee
Keyword(s):  
Oryza Sativa ◽  
Drought Stress ◽  
Plant Stress ◽  
Cereal Crops ◽  
Rice Seedlings ◽  
Drought Tolerant ◽  
Genome Wide ◽  
Enhanced Expression ◽  
Plant Stress Tolerance ◽  
Genome Wide Study

Abstract Rice is one of the important cereal crops mainly cultivated in Asia and its productivity is severely affected by drought stress. In response to drought stress, several genes are reported to be up-regulated or down-regulated in plants. Gene expression is negatively regulated by non-coding endogenous microRNAs post-transcriptionally either by mRNA degrading or translational silencing. In the past, single or multiple stress-responsive genes were over-expressed in order to generate drought-tolerant transgenic rice but with very little success. Recently, the development of transgenic plants by over-expressing transcription factors have received much attention because of their ability to regulate several genes. Abscisic Acid Insensitive 3 (ABI3) is a transcription factor, which is known to play a crucial role in mediating plant stress tolerance. Using the Ensembl plants database, we identified 83 putative OsABI3 genes in Oryza sativa Indica. Through in silico approach, five potential miRNAs i.e., ath-miR5021, csi-miR3948, osa-miRf11773-akr, osa-miRf12029-akr and ptc-miRf10053-akr that target OsABI3 genes were identified. Further, the expression of the selected ABI3 genes were analyzed in rice seedlings exposed to 15% PEG, using the RT-qPCR. In comparison to control, OsABI3 genes showed relatively enhanced expression when exposed to drought stress treatment. This indicates that OsABI3 genes may play important role in development and drought stress in rice seedlings.

scholarly journals Role and Regulation of Cytokinins in Plant Response to Drought Stress

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 422 ◽  
Author(s):  
Nguyen Ngoc Hai ◽  
Nguyen Nguyen Chuong ◽  
Nguyen Huu Cam Tu ◽  
Anna Kisiala ◽  
Xuan Lan Thi Hoang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Association Studies ◽  
Plant Stress ◽  
Genome Wide Association Studies ◽  
Plant Tolerance ◽  
In Planta ◽  
Plant Growth And Development ◽  
Drought Tolerant ◽  
Genome Wide ◽  
Cellular Communication Network

Cytokinins (CKs) are key phytohormones that not only regulate plant growth and development but also mediate plant tolerance to drought stress. Recent advances in genome-wide association studies coupled with in planta characterization have opened new avenues to investigate the drought-responsive expression of CK metabolic and signaling genes, as well as their functions in plant adaptation to drought. Under water deficit, CK signaling has evolved as an inter-cellular communication network which is essential to crosstalk with other types of phytohormones and their regulating pathways in mediating plant stress response. In this review, we revise the current understanding of CK involvement in drought stress tolerance. Particularly, a genetic framework for CK signaling and CK crosstalk with abscisic acid (ABA) in the precise monitoring of drought responses is proposed. In addition, the potential of endogenous CK alteration in crops towards developing drought-tolerant crops is also discussed.

scholarly journals Genome wide identification and characterization of light-harvesting Chloro a/b binding (LHC) genes reveals their potential role in enhancing drought tolerance in Gossypium hirsutum

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Teame Gereziher MEHARI ◽  
Yanchao XU ◽  
Richard Odongo MAGWANGA ◽  
Muhammad Jawad UMER ◽  
Joy Nyangasi KIRUNGU ◽  
...  
Keyword(s):  
Drought Stress ◽  
Gossypium Hirsutum ◽  
Abiotic Stresses ◽  
Substitution Rate ◽  
Synonymous Substitution ◽  
Synonymous Substitution Rate ◽  
Drought Tolerant ◽  
Genome Wide ◽  
Identification And Characterization

Abstract Background Cotton is an important commercial crop for being a valuable source of natural fiber. Its production has undergone a sharp decline because of abiotic stresses, etc. Drought is one of the major abiotic stress causing significant yield losses in cotton. However, plants have evolved self-defense mechanisms to cope abiotic factors like drought, salt, cold, etc. The evolution of stress responsive transcription factors such as the trihelix, a nodule-inception-like protein (NLP), and the late embryogenesis abundant proteins have shown positive response in the resistance improvement to several abiotic stresses. Results Genome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding (LHC) genes were carried out in cotton under drought stress conditions. A hundred and nine proteins encoded by the LHC genes were found in the cotton genome, with 55, 27, and 27 genes found to be distributed in Gossypium hirsutum, G. arboreum, and G. raimondii, respectively. The proteins encoded by the genes were unevenly distributed on various chromosomes. The Ka/Ks (Non-synonymous substitution rate/Synonymous substitution rate) values were less than one, an indication of negative selection of the gene family. Differential expressions of genes showed that majority of the genes are being highly upregulated in the roots as compared with leaves and stem tissues. Most genes were found to be highly expressed in MR-85, a relative drought tolerant germplasm. Conclusion The results provide proofs of the possible role of the LHC genes in improving drought stress tolerance, and can be explored by cotton breeders in releasing a more drought tolerant cotton varieties.

Sucrose Transporter Gene AtSUC4 Responds to Drought Stress by Regulating the Sucrose Distribution and Metabolism in Arabidopsis thaliana

2013 ◽  
Vol 765-767 ◽  
pp. 2971-2975 ◽  
Author(s):  
Xue Gong ◽  
Ming Li Liu ◽  
Li Jun Zhang ◽  
Wei Liu ◽  
Che Wang
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Plant Stress ◽  
Homozygous Mutation ◽  
Transporter Gene ◽  
Wild Type ◽  
Sucrose Transporters ◽  
Whole Plant ◽  
Plant Stress Tolerance ◽  
Plant Level

Sucrose transporters (SUCs or SUTs) are considered as the important carriers and responsible for the loading, unloading and distribution of sucrose, but at present there is no report that SUCs are involved in sucrose distribution and metabolism under drought stress at the whole-plant level. AtSUC4, as the unique member of SUT4-clade inArabidopsis thaliana, may be important for plant stress tolerance. Here, by analyzing two homozygous mutation lines ofAtSUC4(Atsuc4-1andAtsuc4-2), we found drought stress induced higher sucrose, lower fructose and glucose contents in shoots, and lower sucrose, higher fructose and glucose contents in roots of these mutants compared with the wild-type (WT), leading to an imbalance of sucrose distribution, fructose and glucose (sucrose metabolites) accumulation changes at the whole-plant level. Thus we believe thatAtSUC4regulates sucrose distribution and metabolism in response to drought stress.

scholarly journals Comparative hormonal regulatory pathway of the drought responses in relation to glutamate-mediated proline metabolism in Brassica napus

2019 ◽  
Author(s):  
Van Hien La ◽  
Bok-Rye Lee ◽  
Md. Tabibul Islam ◽  
Sang-Hyun Park ◽  
Dong-Won Bae ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Responses ◽  
Plant Stress ◽  
Proline Accumulation ◽  
List Type ◽  
Dependent Manner ◽  
Proline Metabolism ◽  
Regulatory Pathway ◽  
Plant Stress Responses ◽  
Enhanced Expression

AbstractProline metabolism influences metabolic and signaling pathway in regulating plant stress responses. This study aimed to characterize the physiological significance of glutamate (Glu)-mediated proline metabolism in the drought stress responses, focusing on the hormonal regulatory pathway. The responses of cytosolic Ca2+ signaling, proline metabolism and redox components to the exogenous application of Glu in well-watered or drought-stressed plants were interpreted in relation to endogenous hormone status and their signaling genes. Drought-enhanced abscisic acid (ABA) were concomitant with ROS and proline accumulation, accompanied by decreased NAD(P)H/NAD(P)+ and GSH/GSSG ratios. Exogenous Glu-feeding under drought resulted in an increase of salicylic acid (SA) with an antagonistic decrease of ABA. Glu-enhanced SA coincided with the highest expression of SA synthesis related gene ICS1 and Ca2+-dependent protein kinase CPK5. SA-enhanced CPK5 expression was closely associated with further enhancement of proline synthesis-related genes (P5CS1, P5CS2, and P5CR) expression. The Glu-activated proline synthesis was responsible for the reset of reducing potential with enhanced expression of redox regulating genes TRXh5 and GRXC9 in a SA-mediated NPR1- and/or PR1-dependent manner. These results clearly indicate that Glu-activated interplay between SA- and CPK5-signaling and Glu-enhanced proline synthesis are crucial in the amelioration of drought stress in B. napus.HighlightDrought-induced oxidative stress and symptom are developed by ABA-dependent mannerGlu-application increases endogenous SA level with an antagonistic decrease of ABADrought-induced proline accumulation was further enhanced by exogenous Glu-applicationGlu-enhanced proline synthesis accompanied with SA-mediated regulatory pathwayGlu-enhanced SA-modulated proline metabolism is an integrated process of redox control

scholarly journals Construction of GmNAC085 vector for future development of drought-tolerant crops

2018 ◽  
Vol 14 (4) ◽  
pp. 645-652
Author(s):  
Hoang Thi Lan Xuan ◽  
Nguyen Binh Anh Thu ◽  
Nguyen Bao Thien Phuc ◽  
Nguyen Phuong Thao
Keyword(s):  
Drought Stress ◽  
Expression Patterns ◽  
Plant Stress ◽  
Plant Responses ◽  
Potential Candidate ◽  
In Planta ◽  
Functional Studies ◽  
Drought Tolerant ◽  
The Difference ◽  
Alignment Analysis

Various members of NAC transcription factor family have been shown to play important roles in regulating plant responses to abiotic stresses, such as drought, cold and salinity. Our previous research on differential expression patterns of twenty three soybean NAC genes (GmNACs) by realtime quantitative PCR suggested a correlation between inducible expression of GmNAC085 and the drought tolerance degree in DT51 and MTD720 soybean cultivars, which presented for the drought-tolerant and the drought-sensitive, respectively. Therefore, the gene has been proposed as a potential candidate for engineering in order to produce new varieties with better drought stress tolerance. However, functional studies of GmNAC085 should be carried out to identify how this transcriptional factor can contribute in the plant stress-responsive pathway. Herein, this paper presented that we have successfully developed a recombinant binary vector carrying full-length cDNA of GmNAC085 and expression of this gene is placed under the control of constitutive promoter CaMV 35S. The generated construct was firstly transformed into E.coli for sequencing the target gene and then the identified genuine construct was transformed into Agrobacterium tumefaciens. This can be used for plant transformation mediated by Agrobacterium in serving for GmNAC085 - related studies using in planta system such as the model plant Arabidopsis and also serving for the development of drought-tolerant crops by genetic engineering. Additionally, results from sequence alignment analysis revealed that GmNAC085s of DT51 and MTD720 had identical nucleotide sequence, thus supporting our hypothesis that difference in GmNAC085 gene expression levels, not the variation in GmNAC085 protein sequence or structure, might cause the difference in plant resistance degree to drought stress in these two soybean varieties.

scholarly journals Comparative Transcriptomics and Co-Expression Networks Reveal Tissue- and Genotype-Specific Responses of qDTYs to Reproductive-Stage Drought Stress in Rice (Oryza sativa L.)

Genes ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 1124
Author(s):  
Jeshurun Asher Tarun ◽  
Ramil Mauleon ◽  
Juan David Arbelaez ◽  
Sheryl Catausan ◽  
Shalabh Dixit ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Drought Stress ◽  
Quantitative Trait Loci ◽  
Differential Expression ◽  
Quantitative Trait ◽  
Allelic Variation ◽  
Oryza Sativa L ◽  
Flag Leaf ◽  
Reproductive Stage ◽  
Drought Tolerant

Rice (Oryza sativa L.) is more sensitive to drought stress than other cereals. To dissect molecular mechanisms underlying drought-tolerant yield in rice, we applied differential expression and co-expression network approaches to transcriptomes from flag-leaf and emerging panicle tissues of a drought-tolerant yield introgression line, DTY-IL, and the recurrent parent Swarna, under moderate reproductive-stage drought stress. Protein turnover and efficient reactive oxygen species scavenging were found to be the driving factors in both tissues. In the flag-leaf, the responses further included maintenance of photosynthesis and cell wall reorganization, while in the panicle biosynthesis of secondary metabolites was found to play additional roles. Hub genes of importance in differential drought responses included an expansin in the flag-leaf and two peroxidases in the panicle. Overlaying differential expression data with allelic variation in DTY-IL quantitative trait loci allowed for the prioritization of candidate genes. They included a differentially regulated auxin-responsive protein, with DTY-IL-specific amino acid changes in conserved domains, as well as a protein kinase with a DTY-IL-specific frameshift in the C-terminal region. The approach highlights how the integration of differential expression and allelic variation can aid in the discovery of mechanism and putative causal contribution underlying quantitative trait loci for drought-tolerant yield.

scholarly journals Genome Wide Identification and Characterization of Light-Harvesting Chloro a/b Binding Genes Reveals their Potential Role in Enhancing Drought Tolerance in Gossypium hirsutum

2020 ◽  
Author(s):  
Teame Gereziher ◽  
Yanchao Xu ◽  
Richard Odongo Magwanga ◽  
Joy Nyangasi Kirungu ◽  
Xiaoyan Cai ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Gossypium Hirsutum ◽  
Natural Fiber ◽  
Light Harvesting ◽  
Drought Tolerant ◽  
Genome Wide ◽  
Lhc Genes ◽  
Identification And Characterization

Abstract BackgroundCotton is an important commercial crop for its valuable source of natural fiber. Its production has undergone a sharp failure because of abiotic stress influences, of significance is drought. Moreover, plants have evolved self-defense mechanisms against the effects of several ways of abiotic factors like drought, salt, cold among others. The evolution of stress responsive transcription factors such as the trihelix, a nodule-inception-like protein (NLP), the late embryogenesis abundant (LEA) proteins among others have shown positive response in improving resistance to several forms of abiotic stress features.ResultsGenome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding (LHC) genes was carried out in cotton under drought stress conditions. A hundred and nine proteins encoded by the LHC genes were found in the cotton genome, with 55, 27, and 27 genes found to be distributed in Gossypium hirsutum, G. arboreum, and G. raimondii, respectively. The proteins encoded by the genes were unevenly distributed in various chromosomes. The Ka/Ks values were less than one, and an indication of negative selection of the gene family. differential expression arrangement of genes was showed with the majority of the genes being highly upregulated in the root tissues in relative to leave and stem tissues. Moreover, more genes were induced in M85 a relative drought tolerant germplasm.Conclusion:The results provide proof of the possible role of the LHC genes in improving drought stress tolerance, and can be explored by cotton breeders in releasing a more drought tolerant cotton germplasms.

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