scholarly journals Detection of Human Papillomavirus Infection in Oral Cancers reported at Dental Facility: Assessing the Utility of FFPE tissues

2021 ◽  
Author(s):  
Gaurav Verma ◽  
Nikita Aggarwal ◽  
Abhishek Tyagi ◽  
Kanchan Vishnoi ◽  
Mohit Jadli ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Oral Cancer ◽  
Epidemiological Data ◽  
Hpv Infection ◽  
Oral Cancers ◽  
Dental Hospital ◽  
Formalin Fixed Paraffin Embedded ◽  
Ffpe Tissues ◽  
Oral Malignancies ◽  
Protocol Standardization

Abstract Incidence of human papillomavirus (HPV)–associated oral cancers is on the rise. However, epidemiological data of this subset of cancers is limited. Dental hospital pose a unique advantage in detection of HPV-positive oral malignancies. We assessed the utility of formalin-fixed, paraffin-embedded (FFPE) tissues, which are readily available, for evaluation of high-risk HPV infection in oral cancer. For protocol standardization, we used 20 prospectively-collected, paired FFPE and fresh tissues of histopathologically-confirmed oral cancer cases reported in Oral Medicine department of a dental hospital for comparative study. Only short-PCRs (~200bp) of DNA isolated using a modified xylene-free method displayed a concordant HPV result. For HPV analysis, we used additional 30 retrospectively-collected FFPE tissues. DNA isolated from these specimens showed an overall 23.4% (11/47) HPV positivity with detection of HPV18. Comparison of HPV positivity from dental hospital FFPE-specimens with overall HPV positivity of freshly-collected oral cancer specimens (n-55) from three cancer care hospitals of the same region showed notable difference (12.7%; 7/55). Further, cancer hospital specimens showed HPV16 positivity and displayed a characteristic difference in reported sub-sites and patient spectrum. Overall, using a xylene-free FFPE-DNA isolation method clubbed with short amplicon PCR, we showed detection of HPV-positive oral cancer in dental hospitals.

scholarly journals Association of Salivary Human Papillomavirus Infection and Oral and Oropharyngeal Cancer: A Meta-Analysis

2020 ◽  
Vol 9 (5) ◽  
pp. 1305 ◽  
Author(s):  
Óscar Rapado-González ◽  
Cristina Martínez-Reglero ◽  
Ángel Salgado-Barreira ◽  
Almudena Rodríguez-Fernández ◽  
Santiago Aguín-Losada ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Oral Cancer ◽  
Oropharyngeal Cancer ◽  
Meta Analysis ◽  
Hpv Infection ◽  
Cochrane Library ◽  
Oropharyngeal Carcinoma ◽  
Hpv Dna ◽  
Start Date ◽  
Oral And Oropharyngeal Cancer

Background. Human papillomavirus (HPV) infection has been recognized as an important risk factor in cancer. The purpose of this systematic review and meta-analysis was to determine the prevalence and effect size of association between salivary HPV DNA and the risk of developing oral and oropharyngeal cancer. Methods. A systematic literature search of PubMed, EMBASE, Web of Science, LILACS, Scopus and the Cochrane Library was performed, without language restrictions or specified start date. Pooled data were analyzed by calculating odds ratios (ORs) and 95% confidence intervals (CIs). Quality assessment was performed using the Newcastle–Ottawa Scale (NOS). Results. A total of 1672 studies were screened and 14 met inclusion criteria for the meta-analysis. The overall prevalence of salivary HPV DNA for oral and oropharyngeal carcinoma was 43.2%, and the prevalence of salivary HPV16 genotype was 27.5%. Pooled results showed a significant association between salivary HPV and oral and oropharyngeal cancer (OR = 4.94; 2.82−8.67), oral cancer (OR = 2.58; 1.67−3.99) and oropharyngeal cancer (OR = 17.71; 6.42−48.84). Significant associations were also found between salivary HPV16 and oral and oropharyngeal cancer (OR = 10.07; 3.65−27.82), oral cancer (OR = 2.95; 1.23−7.08) and oropharyngeal cancer (OR = 38.50; 22.43−66.07). Conclusions. Our meta-analysis demonstrated the association between salivary HPV infection and the incidence of oral and oropharyngeal cancer indicating its value as a predictive indicator.

scholarly journals Exploring lay public and dental professional knowledge around HPV transmission via oral sex and oral cancer development

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Mario A. Brondani ◽  
Adriana B. Siqueira ◽  
Claudia Maria Coelho Alves
Keyword(s):  
Human Papillomavirus ◽  
Oral Cancer ◽  
Health Care Professionals ◽  
Oropharyngeal Cancer ◽  
Hpv Infection ◽  
Cancer Development ◽  
Oral Sex ◽  
Sexual Activities ◽  
Lay Public ◽  
Dental Professionals

Abstract Background Human papillomavirus (HPV) has been associated with certain types of oropharyngeal cancers and yet, the level of knowledge that dental professionals and the lay public have in terms of HPV transmission, oral sexual activities, and oral cancer development needs exploration. The aim of this study was to assess the knowledge held by practicing dental professionals as well as the lay public regarding Human Papillomavirus (HPV) transmission through oral sex and subsequent oropharyngeal cancer development. Methods Textual data were collected from a public forum with dental professionals in. Vancouver, who discussed the HPV-oral sex-oral cancer triad, and from survey data gathered from 212 lay public participants (also in Vancouver) who answered a 13-item questionnaire on the perceived risks of oral sex in terms of HPV infection and oropharyngeal cancer development. The data were analyzed statistically by age group, gender, and sexual orientation using descriptive statistics, while an ANOVA test was used to compare variation in the responses to the survey (p-value = 0.05). Results The forum engaged 46 health care professionals, many of whom were aware of the potential risks for head and neck cancer development due to HPV infection, while also questioning “how to effectively talk about HPV with patients.” The survey revealed that 34.5% of the participants believed that oral sex is an activity with no or low risk for the transmission of HPV, while 84% of participants believed the same sexual practices were of low or no-risk for HIV (Human Immunodeficiency Virus) transmission. Most participants (82%) never discussed oral sexual activities with their physicians or dentists/dental hygienists. Conclusions The general public remains mostly unaware of the potential links between HPV infection and oropharyngeal cancer. Physicians and dental providers should discuss oral sexual practice with their patients to raise awareness.

Are Muslim women less susceptible to oncogenic human papillomavirus infection? A study from rural eastern India

2004 ◽  
Vol 14 (2) ◽  
pp. 293-303 ◽  
Author(s):  
C. Duttagupta ◽  
S. Sengupta ◽  
M. Roy ◽  
D. Sengupta ◽  
P. Bhattacharya ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Muslim Women ◽  
Epidemiological Data ◽  
Hpv Infection ◽  
Abnormal Cytology ◽  
Papillomavirus Infection ◽  
Atypical Cells ◽  
Hindu Women ◽  
Undetermined Significance

Muslim women are known to have lower incidences of cervical cancer and/or human papillomavirus (HPV) infection. Here we aim to determine any association that may be present between the oncogenic HPV16/18 infections and abnormal cytological lesions along with demographic and other attributes among Indian Muslim women (n = 478) and compare with the neighboring Hindus (n = 534) from a prospective cohort study. Agewise distribution of both subject-groups is similar. HPV16/18 infection is present in 9.6% Muslims and 7.5% Hindu women. Jointly atypical cells of undetermined significance (a typical cells of undetermined significance) and HPV16/18 are present in seven Muslim and two Hindu women. No high squamous intraepithelial lesions or cervical cancer is detected at the baseline. HPV16/18 infections show trends that varied with age, a nonlinear trend among Muslim women. In Hindu women the prevalence is highest at age ≤24 years, which linearly drops with increasing age. Abnormal cytology increases significantly in both religion-groups with increasing age. The data show that these Indian Muslim women are equally susceptible to HPV16/18 infection and for the development of abnormal cytology. There is a paucity in epidemiological data, which justifies the need to screen women of all religions for cervical cancer (that includes oncogenic HPV testing).

scholarly journals Anal human papillomavirus infection and its relations with abnormal anal cytology among MSM with or without HIV infection in Japan

2021 ◽  
Author(s):  
Daisuke Shiojiri ◽  
Daisuke Mizushima ◽  
Misao Takano ◽  
Koji Watanabe ◽  
Naokatsu Ando ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Hiv Infection ◽  
Hpv Vaccination ◽  
Epidemiological Data ◽  
Hpv Infection ◽  
Abnormal Cytology ◽  
Hpv Genotypes ◽  
Anal Cytology ◽  
The Difference

Abstract Background Anorectal high-risk human papillomavirus (hr-HPV) infection is associated with anal malignancies. However, the epidemiological data is quite limited in Japan. Methods Anorectal swab was obtained from HIV-infected and –uninfected men who have sex with men (MSM). Only high-risk HPV genotypes were examined by the Hybrid Capture-based methodology. Anal cytology was determined by the modification of the Bethesda System classification. Results In total, 644 participants were included. No participant had history of HPV vaccination at inclusion. The overall prevalence of hr-HPV was 58.5% (95% confidence interval: 54.7-62.3). The most frequently detected hr-HPV genotypes in order were HPV-52, -16, and -58. Among the HPV infected subjects, 82.8% (312/377) were infected with at least one of the 9 valent vaccine covered genotypes. Incidence of abnormal cytology was positively correlated with the number of HPV genotypes infected rather than the difference in genotypes. Multivariate analysis identified the number of detected hr-HPV genotypes as well as HIV-infection were the independent risk factors for pre-cancer. Conclusion Nine valent vaccine preventable HPVs are currently prevalent among MSM in Japan. Also, increased numbers of hr-HPV genotypes were strongly associated with anorectal pre-cancer. Universal HPV vaccination should be considered for Japanese MSM.

scholarly journals Results of a vaccination campaign against human papillomavirus in the province of La Spezia, Liguria, Italy, March-December 2008

2009 ◽  
Vol 14 (39) ◽  
Author(s):  
J Lugarini ◽  
F Maddalo
Keyword(s):  
Human Papillomavirus ◽  
Sexually Transmitted Diseases ◽  
Cervical Carcinoma ◽  
Vaccination Campaign ◽  
Hpv Vaccination ◽  
Target Population ◽  
Hpv Infection ◽  
Sexually Transmitted ◽  
Oral Cancers ◽  
The Ideal

Sexually transmitted diseases caused by human papillomavirus (HPV) are being diagnosed more frequently than others. It is accepted that HPV infection is a necessary cause for all cases of cervical carcinoma and a large number of other anogenital and oral cancers. Two vaccines have been developed and were licensed in 2007, which can prevent infections and pre-cancerous lesions due to HPV. In Italy pre-adolescent age (12 years-old) was identified as the ideal age for vaccination against HPV. In Liguria, the first free HPV vaccination campaign was started on 8 March 2008 in 12 year-old girls. We assessed the adherence to the vaccination during the 2008 campaign as 80.6%, 79.0% and 64.1%, respectively, for the first, second and third dose of vaccine in the target population.

scholarly journals Oral sex and oral cancer in the context of human papillomavirus infection: lay public understanding

2011 ◽  
Vol 4 (3) ◽  
pp. 171
Author(s):  
Mario A. Brondani ◽  
Mario A. Cruz-Cabrera ◽  
Cheryle Colombe
Keyword(s):  
Human Papillomavirus ◽  
Sexual Orientation ◽  
Oral Cancer ◽  
Small Sample Size ◽  
Hpv Infection ◽  
Small Sample ◽  
Public Understanding ◽  
Sexual Practices ◽  
Cancer Development ◽  
Oral Sex

Human papillomavirus (HPV) is a risk factor for ano-genital and cervical cancers and has been associated with head and neck malignancies in the context of oral sex for the transmission of the virus. However, the level of knowledge that lay people have in terms of HPV transmission through oral sex and oral cancer development remains unknown. A pilot sample of 150 questionnaires was distributed at specific non-profit health organizations in Vancouver, Canada. Questions included perceived risks for oral sex in terms of HPV infection and oral cancer development, and the frequency with which respondents were asked about oral sexual practices by physicians and dentists. Data were analysed statistically by age group (19– 30, 31–50, 50\), gender (male, female), and sexual orientation (queer, straight). 110 questionnaires were returned fully completed. For the transmission of HPV, 58% of the participants believed that oral sex is an activity of no or low risk, whereas 72% considered the same activity to be of no risk for the development of oral cancer. There was no statistical difference between gender and sexual orientation. Participants never discussed related health risks in regard to oral sex with their physicians or dentists. In conclusion, although recent attention has been given to the potential links between HPV infection and oral cancer, such links remain mostly unknown by the public. Physicians and dentists could discuss oral sex practices to raise awareness with their patients. This is a small sample size study and the results should be interpreted with caution.

The epidemiology of anal human papillomavirus infection among women and men having sex with women

Sexual Health ◽  
2012 ◽  
Vol 9 (6) ◽  
pp. 538 ◽  
Author(s):  
Alan G. Nyitray
Keyword(s):  
Human Papillomavirus ◽  
Anal Canal ◽  
Hpv Vaccination ◽  
Epidemiological Data ◽  
Hpv Infection ◽  
Common Finding ◽  
Genotype Distribution ◽  
Anal Sex ◽  
Lifetime Number ◽  
Receptive Anal Sex

The goal of this review is to summarise epidemiological data that support an understanding of the natural history of anal human papillomavirus (HPV) among women and men having sex with women (MSW). HPV is a common infection of the anal canal among women and MSW. Although there have been a limited number of studies to date, both oncogenic and nononcogenic HPV genotypes commonly occur among these populations even when individuals do not report receptive anal sex. Genotype distribution is quite diverse, with recent studies typically detecting more than two dozen genotypes in the anal canal in samples of women and MSW. Factors most consistently associated with HPV in the anal canal among both women and MSW are lifetime number of sexual partners and detection of HPV at the genitals. The common finding of genotypic concordance between the genitals and anal canal in women and MSW, and the infectious nature of HPV, in addition to a limited number of studies offering empirical evidence of anal-to-genital self-inoculation and evidence of HPV hand carriage, may help explain the detection of HPV in the anal canal outside the context of receptive anal sex. HPV vaccination has been shown to reduce anal HPV infection among women and is also a promising prevention strategy among MSW.

scholarly journals Human papillomavirus DNA prevalence and type distribution in oral squamous cell carcinoma in Ghana

2018 ◽  
Vol 3 ◽  
pp. 2057178X1878712
Author(s):  
Richardar N Taylor Dawson ◽  
Nii Otu Nartey ◽  
Francis Kwamin ◽  
Ebenezer A Nyako ◽  
Richard H Asmah ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
High Risk ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Nested Polymerase Chain Reaction ◽  
Hpv Dna ◽  
Oral Cancers ◽  
Formalin Fixed Paraffin Embedded

Objective: To determine the prevalence of human papillomavirus (HPV) DNA in oral squamous cell carcinoma (OSCC). Methods: A total of 88 OSCC specimens collected between 2006 and 2013 were available for the study. DNA was extracted using formalin-fixed, paraffin-embedded specimens and analysed for the presence of 18 HPV genotypes using a nested polymerase chain reaction using consensus forward primer (GP-E6-3F) and two consensus back primers (GP-E7-5B and GP-E7-6B). Plasmid DNA of HPV 16 and 18 was used as positive controls. Results: HPV DNA was detected in 3 of the 88 samples, a prevalence of 3.4%. Genotypes detected were 16, 18 and 52. Conclusion: The overall prevalence of HPV DNA was 3.4%. Only high-risk genotypes were detected. This low prevalence of high-risk types of HPV suggests that the HPV virus may not have a significant role in the development of oral cancers in Ghana, unlike higher rates described elsewhere in the world, especially in Western countries. Surveillance of future prevalence of HPV and attention to other major risk factors is warranted.

scholarly journals Retrospective study of Bovine herpesvirus 5 meningoencephalitis in cattle from São Paulo State, Brazil

2017 ◽  
Vol 69 (2) ◽  
pp. 299-304 ◽  
Author(s):  
D.Q. Cagnini ◽  
D.G.A. Andrade ◽  
P.H.J. Cunha ◽  
J.P. Oliveira-Filho ◽  
R.L. Amorim ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Bovine Herpesvirus ◽  
Epidemiological Data ◽  
Large Animal ◽  
Good Tool ◽  
Glycoprotein C ◽  
Neurological Signs ◽  
Formalin Fixed Paraffin Embedded ◽  
Ffpe Tissues ◽  
Cattle Herds

ABSTRACT Meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5) is an important neurological disease that affects Brazilian cattle herds. The present study investigated the presence of BoHV-5 DNA in cattle diagnosed with meningoencephalitis at Faculdade de Medicina Veterinária e Zootecnia, UNESP - Univ Estadual Paulista from 1980 to 2009. The records obtained from the Large Animal Internal Medicine Service and the Animal Pathology Service were reviewed to identify clinical and epidemiological data from cattle with neurological signs. Excluding rabies cases, we found 115 cases of cattle with neurological signs that had been necropsied. Non-suppurative meningoencephalitis was diagnosed in 28 animals of the 115 initially selected based on histopathological examination of brain tissues. Of these 28 animals, 15 (54%) were positive for BoHV-5 DNA by polymerase chain reaction (PCR) of formalin-fixed paraffin-embedded (FFPE) brain samples. PCR target was 159-bp fragment from the BoHV-5 glycoprotein C gene. The oldest case identified in the present study was from 1988. PCR was a good tool for the diagnosis of BoHV-5 DNA extracted from FFPE tissues, allowing retrospective studies of samples stored for more than 20 years.

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