scholarly journals Fine Mapping and Cloning of the Novel Gene Qph-IAA30, which Simultaneously Affects the Plant Height, Panicle Length, Spikelet Number and Yield in Rice (Oryza Sativa L.)

2021 ◽  
Author(s):  
Niqing He ◽  
Guangping Zhan ◽  
Fenghuang Huang ◽  
Xianghua Zheng ◽  
Chaoping Cheng ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Plant Height ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Gene Prediction ◽  
Rice Genome ◽  
Rice Cultivar ◽  
Rice Cultivars ◽  
Panicle Length ◽  
Higher Plant

Abstract Background: The plant height is one of the most important agronomic traits in rice (Oryza sativa L.), and the introduction of semidwarf rice led to record yield increases throughout Asia in the 1960s. Near-isogenic lines (NILs) are the most powerful tools for the detection and precise mapping of quantitative trait loci (QTLs).Results: In this study, 176 NILs were produced from the crossing and back-crossing of two rice cultivars. Specifically, Jiafuzhan, an indica rice cultivar, served as the recipient, and Hui1586, a restorer japonica cultivar, served as the donor. Using the 176 NILs, we identified a novel QTL for plant height in NIL36. First, we mapped the QTL to a 31-kb region between the markers Indel12-29 and Indel12-31. The rice genome annotation indicated the presence of three candidate genes in this region. Through gene prediction and cDNA sequencing, we confirmed that the target gene in NIL36 was Osiaa30, hereafter referred to as qPH-iaa30. Further analysis showed that qPH-iaa30 was produced by a 1-bp deletion in the first exon that resulted in the premature termination of OsIAA30. Knockout experiments showed that qPH-IAA30 was responsible for the plant height phenotype. Although qPH-IAA30 from Jiafuzhan showed a higher plant height, the plant also exhibited a longer panicle length, more spikelets and a higher yield. Taken together, our results demonstrate that qPH-IAA30 has good specific application prospects in future rice breeding.Conclusions: 176 NILs are produced from two rice cultivars, using the 176 NILs, a novel qPH-iaa30 for plant height is identified, and the qPH-IAA30 gene is responsible for the plant height phenotype.

scholarly journals Daya Gabung Beberapa Varietas Padi (Oryza sativa L.) pada Persilangan Dialel Menggunakan Metode Griffing II

2021 ◽  
pp. 84-93
Author(s):  
Anak Agung Ketut Sudharmawan ◽  
Baiq Erna Listiana ◽  
Sofi Rianti
Keyword(s):  
Oryza Sativa ◽  
Plant Height ◽  
Power Analysis ◽  
Oryza Sativa L ◽  
Panicle Length ◽  
Grain Number ◽  
Significant Difference ◽  
Diallel Crossing ◽  
High Yielding Varieties ◽  
Materials Used

Diallel crossing is a method that is widely used to determine the ability to combine each individual so that it can identify the potential elders to be used in crossbreeding programs to assemble high yielding varieties. This experiment aims to determine the combining power of dialelic crosses of several varieties of rice using the Griffing II method, carried out in the experimental field of the Faculty of Agriculture, Mataram University in Nyiur Lembang Village, Narmada District, West Lombok Regency, from February to July 2019. This experiment uses the method experiments carried out in the field. The materials used in this experiment were four parents, namely IPB 3S variety, Situ Patenggang variety, Inpari 32 and G11 strain and the phenotype of the cross between the four parents. The experimental results showed that the effects of general affinity and special affinity for all observed characters (plant height, number of productive tillers, number of non-productive tillers, panicle length, number of filled grain, number of empty grain and weight of 100 grains) were significantly different, except on the effect of general affinity for the number of non-productive tillers showed no significant difference. The results of the combined power analysis of the four elders were positive, so it can be said that the four elders would get good results when crossed with a number of other genotypes.

scholarly journals Cloning of long sterile lemma (lsl2), a single recessive gene that regulates spike germination in rice (Oryza sativa L.)

2020 ◽  
Author(s):  
dewei yang ◽  
Niqing He ◽  
Xianghua Zheng ◽  
Yanmei Zhen ◽  
Zhenxin Xie ◽  
...  
Keyword(s):  
Seed Germination ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Gene Prediction ◽  
Germination Rate ◽  
Rice Genome ◽  
Recessive Gene ◽  
Floral Organ ◽  
Monocotyledonous Plant ◽  
Sterile Lemma

Abstract Background: Rice is a typical monocotyledonous plant and an important cereal crop. The structural units of rice flowers are spikelets and florets, and floral organ development and spike germination affect rice reproduction and yield.Results: In this study, we identified a novel long sterile lemma (lsl2) mutant from an EMS population. First, we mapped the lsl2 gene between the markers Indel7-22 and Indel7-27, which encompasses a 25-kb region. The rice genome annotation indicated the presence of four candidate genes in this region. Through gene prediction and cDNA sequencing, we confirmed that the target gene in the lsl2 mutant is allelic to LONG STERILE LEMMA1 (G1)/ELONGATED EMPTY GLUME (ELE), hereafter referred to as lsl2. Further analysis of the lsl2 and LSL2 proteins showed a one-amino-acid change, namely, the mutation of serine (Ser) 79 to proline (Pro) in lsl2 compared with LSL2, and this mutation might change the function of the protein. Knockout experiments showed that the lsl2 gene is responsible for the long sterile lemma phenotype. The lsl2 gene might reduce the damage induced by spike germination by decreasing the seed germination rate, but other agronomic traits of rice were not changed in the lsl2 mutant. Taken together, our results demonstrate that the lsl2 gene will have specific application prospects in future rice breeding.Conclusions: The lsl2 gene is responsible for the long sterile lemma phenotype and might reduce the damage induced by spike germination by decreasing the seed germination rate.

scholarly journals Cloning of long sterile lemma (lsl2), a single recessive gene that regulates spike germination in rice (Oryza sativa L.)

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Dewei Yang ◽  
Niqing He ◽  
Xianghua Zheng ◽  
Yanmei Zhen ◽  
Zhenxin Xie ◽  
...  
Keyword(s):  
Seed Germination ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Gene Prediction ◽  
Germination Rate ◽  
Rice Genome ◽  
Recessive Gene ◽  
Floral Organ ◽  
Monocotyledonous Plant ◽  
Sterile Lemma

Abstract Background Rice is a typical monocotyledonous plant and an important cereal crop. The structural units of rice flowers are spikelets and florets, and floral organ development and spike germination affect rice reproduction and yield. Results In this study, we identified a novel long sterile lemma (lsl2) mutant from an EMS population. First, we mapped the lsl2 gene between the markers Indel7–22 and Indel7–27, which encompasses a 25-kb region. The rice genome annotation indicated the presence of four candidate genes in this region. Through gene prediction and cDNA sequencing, we confirmed that the target gene in the lsl2 mutant is allelic to LONG STERILE LEMMA1 (G1)/ELONGATED EMPTY GLUME (ELE), hereafter referred to as lsl2. Further analysis of the lsl2 and LSL2 proteins showed a one-amino-acid change, namely, the mutation of serine (Ser) 79 to proline (Pro) in lsl2 compared with LSL2, and this mutation might change the function of the protein. Knockout experiments showed that the lsl2 gene is responsible for the long sterile lemma phenotype. The lsl2 gene might reduce the damage induced by spike germination by decreasing the seed germination rate, but other agronomic traits of rice were not changed in the lsl2 mutant. Taken together, our results demonstrate that the lsl2 gene will have specific application prospects in future rice breeding. Conclusions The lsl2 gene is responsible for the long sterile lemma phenotype and might reduce the damage induced by spike germination by decreasing the seed germination rate.

scholarly journals Pengaruh Pola Tanam Terhadap Pertumbuhan Gulma dan Komponen Produksi Tanaman Padi Sawah (Oryza sativa L.)

Jurnal Agrium ◽  
2017 ◽  
Vol 14 (2) ◽  
pp. 9
Author(s):  
Irwansyah Irwansyah ◽  
Bhaidawi Bhaidawi ◽  
Muhammad Yusuf N
Keyword(s):  
Oryza Sativa ◽  
Plant Height ◽  
Oryza Sativa L ◽  
Block Design ◽  
Cropping Pattern ◽  
Panicle Length ◽  
Cropping Patterns ◽  
Weed Growth ◽  
Randomized Block Design ◽  
Planting System

The principle of planting system “Jajar Legowo” is to increase the plant population by adjusting the spacing so that the crop will have a row of plants interspersed by empty rows where the spacing is on the row of rows half the spacing between rows. The purpose of this study to investigate the effect of different rice cropping patterns on weed growth. The research was conducted at Gampong Lhok Iboh, Baktiya district Barat North Aceh Regency started from March to June 2017. This research used non factorial Randomized Block Design which consists factor with 2 treatments: P1 (conventional cropping pattern) and P2 (jajar legowo). Observations included the amount of dominance (%), plant height (cm), number of tillers, panicle length, number of grains per panicle, and weight of 1000 grains per plot. The results revealed that cropping pattern had an effect on weed growth and production component of paddy. Planting system “jajar legowo” 2: 1 (25cm x 25 cm x 40 cm) was much better compared to conventional cropping pattern

scholarly journals Genetic Diversity of Local Rainfed Rice (Oryza Sativa L.)

1970 ◽  
Vol 23 (2) ◽  
pp. 41-46 ◽  
Author(s):  
MA Siddique ◽  
ESMH Rashid ◽  
M Khalequzzaman ◽  
MZ Islam ◽  
MS Ahmed ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Plant Height ◽  
Oryza Sativa L ◽  
Flag Leaf ◽  
Mean Value ◽  
Leaf Length ◽  
Panicle Length ◽  
Rainfed Rice ◽  
Cluster Distance

Genetic diversity of 58 traditional local rice genotypes was studied under rainfed condition through Mahalanobis D2 statistic for grain yield and yield contributing characters. The genotypes were grouped into nine clusters. The inter-cluster distances were higher than intra-cluster distances indicating wider genetic diversity among the genotypes of different clusters. The intra-cluster distances were lower in all the cases reflecting homogeneity of the genotypes within the clusters. The cluster III contained the highest number of genotypes (13) and the cluster I contained the lowest (2). The highest intra-cluster distance was noticed for the cluster I and the lowest for cluster VII. The highest inter-cluster distance was observed between cluster I and IV followed by cluster IX and IV, cluster I and VI and the lowest between cluster V and III. Regarding inter-cluster distance, the genotypes of cluster IV showed high genetic distance from all other clusters. The genotypes from cluster IV could be hybridized with the genotypes of other characters of other clusters for producing transgressive segregants. Based on positive value of vector 1 and vector 2, flag leaf length, plant height, panicle length and grain breath had maximum contribution towards genetic divergence. The highest cluster means for yield, 1000 grain weight and grain breath were obtained from cluster VIII. The highest plant height, flag leaf width and panicle length were found in cluster II while the lowest mean value for yield, days to flowering and maturity, unfilled grain and the highest mean value for grain length breadth ratio were found in cluster II. Therefore, more emphasis should be given on cluster IV and cluster VIII, cluster I and cluster IV for selecting genotypes as parents for crossing with the genotypes of cluster II which would be used to produce new recombinants with desired traits.   Key words: Genetic diversity; D2 analysis; cluster analysis; rice (Oryza sativa L)DOI: http://dx.doi.org/10.3329/bjpbg.v23i2.9324 Bangladesh J. Pl. Breed. Genet., 23(2): 41-46, 2010

Impact of seasonal changes on spikelets per panicle, panicle length and plant height in rice (Oryza sativa L.)

Euphytica ◽  
2011 ◽  
Vol 179 (2) ◽  
pp. 319-331 ◽  
Author(s):  
Mallikarjuna Rao Kovi ◽  
Xufeng Bai ◽  
Donghai Mao ◽  
Yongzhong Xing
Keyword(s):  
Oryza Sativa ◽  
Plant Height ◽  
Seasonal Changes ◽  
Oryza Sativa L ◽  
Panicle Length ◽  
Spikelets Per Panicle

scholarly journals Effects of Ethyl methanesulfonate (EMS) on Seedling and Yield Contributing Traits in Basmati Rice

2021 ◽  
Author(s):  
Muhammad Rashid ◽  
Areeqa Shamshad ◽  
Ljupcho Jankuloski
Keyword(s):  
Oryza Sativa ◽  
Plant Height ◽  
Root Length ◽  
Oryza Sativa L ◽  
Shoot Length ◽  
Chemical Mutagenesis ◽  
Panicle Length ◽  
Ethyl Methane ◽  
Chemical Mutagens ◽  
Sterile Spikelet

Increasing genetic diversity in crop plants has been used for chemical mutagenesis. Through the application of various mutagenic agents, over 430 new varieties have been derived as rice mutants (Oryza sativa L.) Chemical mutagens such as ethyl methane sulphonate (EMS), diepoxybutane derivative (DEB), sodium azide, and gamma ray, x-ray, and quick neutron irradiation have been commonly used to induce a large number of functional variations in rice and others crops. Among chemical mutagens, ethyl methane sulfonate (EMS) is the alkylating agent most widely used in plants because it induces nucleotide substitutions to be extremely frequent, as detected in various genomes. In this study, seeds of potential genotype of the popular variety, (Oryza sativa L. Super Basmati variety) were treated with EMS at concentrations of 0.25%, 0.50%, 0.75%, 1% and 1.5%. Various measurements on the M1 generation determined EMS sensitivity. As concentration of applied EMS increased, will decrease in germination, shoot length, root length, plant height, productive tillers, Panicle Length, Total Spikelet, sterile spikelet and fertility under field conditions were observed in M1 generation as compared to the non-treatment control. Emergence, shoot length, root length, plant height, productive tillers, Panicle Length, Total Spikelet, sterile spikelet and fertility also decreased with increases in EMS mutagenesis in an approximately linear fashion. The LD50 values were observed based on growth reduction of seedlings after EMS treatment with 0.25% and 0.50% on the rice variety (Oryza sativa L. spp.).

scholarly journals Cloning of long sterile lemma (lsl2), a single recessive gene regulating spike germination in rice (Oryza sativa L.)

2020 ◽  
Author(s):  
Dewei Yang ◽  
Niqing He ◽  
Xianghua Zheng ◽  
Yanmei Zhen ◽  
Zhenxin Xie ◽  
...  
Keyword(s):  
Seed Germination ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Gene Prediction ◽  
Germination Rate ◽  
Rice Genome ◽  
Recessive Gene ◽  
Floral Organ ◽  
Monocotyledonous Plant ◽  
Sterile Lemma

Abstract Background: Rice is a typical monocotyledonous plant and an important cereal crop. The structural units of rice flowers are spikelets and florets, and floral organ development and spike germination affect rice reproduction and yield.Results: In this study, we identified a novel long sterile lemma (lsl2) mutant from an EMS population. First, we mapped the lsl2 gene between the markers Indel7-22 and Indel7-27, which encompasses a 25-kb region. The rice genome annotation indicated the presence of four candidate genes in this region. Through gene prediction and cDNA sequencing, we confirmed that the target gene in the lsl2 mutant is allelic to LONG STERILE LEMMA1 (G1)/ELONGATED EMPTY GLUME (ELE), hereafter referred to as lsl2. Further analysis of the lsl2 and LSL2 proteins showed a one-amino-acid change, namely, the mutation of serine (Ser) 79 to proline (Pro) in lsl2 compared with LSL2, and this mutation might change the function of the protein. Knockout experiments showed that the lsl2 gene is responsible for the long sterile lemma phenotype. The lsl2 gene might reduce the damage induced by spike germination by decreasing the seed germination rate, but other agronomic traits of rice were not changed in the lsl2 mutant. Taken together, our results demonstrate that the lsl2 gene will have specific application prospects in future rice breeding.Conclusions: The lsl2 gene is responsible for the long sterile lemma phenotype and might reduce the damage induced by spike germination by decreasing the seed germination rate.

scholarly journals Identification of variability for agronomically important traits in rice mutant families

Bragantia ◽  
2016 ◽  
Vol 75 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Viviane Kopp da Luz ◽  
Solange Ferreira da Silveira Silveira ◽  
Gabriela Magalhães da Fonseca ◽  
Eder Licieri Groli ◽  
Ricardo Garcia Figueiredo ◽  
...  
Keyword(s):  
Plant Height ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Flag Leaf ◽  
Grain Weight ◽  
Superior Performance ◽  
Yield Potential ◽  
Panicle Length ◽  
Rice Mutant ◽  
Panicle Weight

ABSTRACT The increase of yield potential in new rice (Oryza sativa L.) varieties has been a major challenge for genetic improvement. The generation of mutants, followed by their characterization, constitutes a great possibility to isolate and select genes and genotypes that present agronomic traits of interest. This study aimed to evaluate the effect of chemical mutagen ethyl methane sulphonate (EMS) on agronomic traits in 340 M3 families of rice derived from BRS Querência cultivar. Seeds from each family and the original genotype BRS Querência were sown in the experimental field, and the characters main panicle length, main panicle weight, main panicle grain weight, flag leaf width and plant height were evaluated. Data were subjected to analysis of variance (p ≤ 0.05), and a comparison of means was carried out by Dunnett's test at 5% significance. The results show that there is genetic variability among the mutant families, suggesting that the mutagen EMS at 1.5% is effective for generating mutants for all assessed traits. Among the characters, plant height was the most affected by the mutagen, which provided an increase in the character. For the main panicle length character, seven families showed means above the control; for main panicle weight and grain weight, four and six mutant families were observed, respectively, with a superior performance in comparison to the control.

scholarly journals I Heritabilitas Galur Padi Beras Hitam (Oryza sativa L) Hasil Seleksi Pedigree F1

2019 ◽  
Vol 5 (1) ◽  
pp. 25
Author(s):  
IGP Muliarta Aryana ◽  
Bambang Budi Santoso ◽  
AAK Sudharmawan ◽  
Muhammad Sukri
Keyword(s):  
Oryza Sativa ◽  
Field Experiment ◽  
Plant Height ◽  
Analysis Of Variance ◽  
Oryza Sativa L ◽  
Research Station ◽  
Panicle Length ◽  
Black Rice ◽  
Pedigree Selection

This study aims to determine Heritability value of F1line of black rice characters result from pedigree selection. This research conducted by field experiment carried out at the research station of Faculty of Agriculture, University of Mataram. Nyur Lembang Village, Narmada District, West Lombok Regency, NTB Province from February to June 2018. This research empioyed Randomized Complete Blok Desing (RCBD) by 40 genotipes as treatments with replications, so there are 80 of experimental units. Observed data analized by analysis of variance (ANOVA). The result of research showed that high of heritability value gained from flowering age plant height, panicle length, number of grains filled, and weight of 100 grains. Low heritability criteria are shown by number of productive tillers, number of empty grains, weight of grains per clumpsand yield (ton.ha-1)

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