Evaluation of reversal and transposition generational damages caused by manganese (II) in zebrafish

Abstract Although manganese (Mn) is a common water pollutant, little is known about the potential for the trans-generational transfer of biological effects in fish exposed to low concentrations in aquatic organisms, or the persistence of these effects. The aim was to identify when biological effects were firstly observed in the brain, liver and intestinal tissues of zebrafish exposed to environmentally relevant concentrations of Mn and whether these effects were subject to reversal when exposures ceased. We also aimed to assess whether biological effects observed in zebrafish exposed to Mn for 21-days were also observed in the early life stages of the F1 generation. Behavioral changes were observed in the animals exposed for 14 days to MnCl2 (4.0 mg L-1). There was also an oscillation in the mitochondrial activity and TUNEL positive cells in the telencephalon of exposed fish from all treatments. These results indicated that brain neurogenesis in zebrafish occurred with greater magnitude and speed than the neuronal Mn damage. Histopathological changes were observed in the intestine and liver of zebrafish exposed to Mn for 72 h and 21 days. Behavioral alterations and intestinal histopathological alterations were reversed after the recovery period, although hepatocyte vacuolization persisted. Behavioral alterations were also observed in the early developmental stages of F1 generation larvae of zebrafish parents exposed to Mn, but this profile was normalized throughout the development. In addition to the direct toxic effects of low concentrations of Mn, some changes, such as hepatocyte vacuolization persisted in zebrafish even after the toxicant removal.

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Evaluation of Reversal and Transposition Generational Damages Caused by Manganese (II) in Zebrafish

10.21203/rs.3.rs-855463/v1 ◽

2021 ◽

Author(s):

Gabriela Zimmermann Prado Rodrigues ◽

Thainá Garbino dos Santos ◽

Mariana Finkler ◽

Jorge Henrique Burghausen ◽

Diulliane De Jesus Borba ◽

...

Keyword(s):

Developmental Stages ◽

Biological Effects ◽

Recovery Period ◽

Aquatic Organisms ◽

Mitochondrial Activity ◽

Behavioral Alterations ◽

Low Concentrations ◽

Water Pollutant ◽

Early Developmental ◽

F1 Generation

Abstract Although manganese (Mn) is a common water pollutant, little is known about the potential for the trans-generational transfer of biological effects in fish exposed to low concentrations in aquatic organisms, or the persistence of these effects. The aim was to identify when biological effects were firstly observed in the brain, liver and intestinal tissues of zebrafish exposed to environmentally relevant concentrations of Mn and whether these effects were subject to reversal when exposures ceased. We also aimed to assess whether biological effects observed in zebrafish exposed to Mn for 21-days were also observed in the early life stages of the F1 generation. Behavioral changes were observed in the animals exposed for 14 days to MnCl 2 (4.0 mg L -1 ). There was also an oscillation in the mitochondrial activity and TUNEL positive cells in the telencephalon of exposed fish from all treatments. These results indicated that brain neurogenesis in zebrafish occurred with greater magnitude and speed than the neuronal Mn damage. Histopathological changes were observed in the intestine and liver of zebrafish exposed to Mn for 72 h and 21 days. Behavioral alterations and intestinal histopathological alterations were reversed after the recovery period, although hepatocyte vacuolization persisted. Behavioral alterations were also observed in the early developmental stages of F1 generation larvae of zebrafish parents exposed to Mn, but this profile was normalized throughout the development. In addition to the direct toxic effects of low concentrations of Mn, some changes, such as hepatocyte vacuolization persisted in zebrafish even after the toxicant removal.

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Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010005161x ◽

1974 ◽

Vol 32 ◽

pp. 320-321

Author(s):

J. P. Revel

Keyword(s):

Developmental Stages ◽

Three Dimensional ◽

Cell Movement ◽

Cellular Interactions ◽

Serial Sections ◽

Cell Sheets ◽

Freeze Etching ◽

Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

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Review of early developmental stages of trilobites and agnostids from the Barrandian area (Czech Republic)

Journal of the National Museum (Prague), Natural History Series ◽

10.2478/jzh-2018-0006 ◽

2017 ◽

Vol 186 (1) ◽

pp. 103-112

Author(s):

Lukáš Laibl ◽

Oldřich Fatka

Keyword(s):

Czech Republic ◽

Developmental Stages ◽

History Of Research ◽

Early Developmental Stages ◽

History Of ◽

Barrandian Area ◽

Early Developmental

This contribution briefly summarizes the history of research, modes of preservation and stratigraphic distribution of 51 trilobite and five agnostid taxa from the Barrandian area, for which the early developmental stages have been described.

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The Influence of Ultra-Low Concentrations of Potassium Anphen on the Bioenergetic Characteristics of Mitochondria

Current Bioactive Compounds ◽

10.2174/1573407214666181116093909 ◽

2020 ◽

Vol 16 (4) ◽

pp. 537-542

Author(s):

Zhigacheva Irina ◽

Volodkin Aleksandr ◽

Rasulov Maksud

Keyword(s):

Functional State ◽

Membrane Lipids ◽

Biological Effects ◽

Dose Dependence ◽

Stress Factors ◽

Mitochondrial Membranes ◽

Oxidation Rates ◽

Pea Seedlings ◽

Low Concentrations ◽

Wide Range

Background: One of the main sources of ROS in stress conditions is the mitochondria. Excessive generation of ROS leads to oxidation of thiol groups of proteins, peroxidation of membrane lipids and swelling of the mitochondria. In this regard, there is a need to search for preparationsadaptogens that increase the body's resistance to stress factors. Perhaps, antioxidants can serve as such adaptogens. This work aims at studying the effect of antioxidant; the potassium anphen in a wide range of concentrations on the functional state of 6 day etiolated pea seedlings mitochondria (Pisum sativum L). Methods: The functional state of mitochondria was studied per rates of mitochondria respiration, by the level of lipid peroxidation and study of fatty acid composition of mitochondrial membranes by chromatography technique. Results: Potassium anphen in concentrations of 10-5 - 10-8 M and 10-13-10-16 prevented the activation of LPO in the mitochondrial membranes of pea seedlings, increased the oxidation rates of NAD-dependent substrates and succinate in the respiratory chain of mitochondria that probably pointed to the anti-stress properties of the drug. Indeed, the treatment of pea seeds with the preparation in concentrations of 10-13 M prevented the inhibition of growth of seedlings in conditions of water deficiency. Conclusion: It is assumed that the dose dependence of the biological effects of potassium anphen and the manifestation of these effects in ultra-low concentrations are due to its ability in water solutions to form a hydrate containing molecular ensembles (structures).

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The Effect of an Anthropogenic Magnetic Field on the Early Developmental Stages of Fishes—A Review

International Journal of Molecular Sciences ◽

10.3390/ijms22031210 ◽

2021 ◽

Vol 22 (3) ◽

pp. 1210

Author(s):

Krzysztof Formicki ◽

Agata Korzelecka-Orkisz ◽

Adam Tański

Keyword(s):

Magnetic Fields ◽

Developmental Stages ◽

Negative Influence ◽

Pigment Cells ◽

Egg Activation ◽

Industrial Equipment ◽

Positive Effects ◽

Early Developmental Stages ◽

Species Specific ◽

Early Developmental

The number of sources of anthropogenic magnetic and electromagnetic fields generated by various underwater facilities, industrial equipment, and transferring devices in aquatic environment is increasing. These have an effect on an array of fish life processes, but especially the early developmental stages. The magnitude of these effects depends on field strength and time of exposure and is species-specific. We review studies on the effect of magnetic fields on the course of embryogenesis, with special reference to survival, the size of the embryos, embryonic motor function, changes in pigment cells, respiration hatching, and directional reactions. We also describe the effect of magnetic fields on sperm motility and egg activation. Magnetic fields can exert positive effects, as in the case of the considerable extension of sperm capability of activation, or have a negative influence in the form of a disturbance in heart rate or developmental instability in inner ear organs.

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Wnt/β-catenin Pathway-Mediated PPARδ Expression during Embryonic Development Differentiation and Disease

International Journal of Molecular Sciences ◽

10.3390/ijms22041854 ◽

2021 ◽

Vol 22 (4) ◽

pp. 1854

Author(s):

Tabinda Sidrat ◽

Zia-Ur Rehman ◽

Myeong-Don Joo ◽

Kyeong-Lim Lee ◽

Il-Keun Kong

Keyword(s):

Embryonic Development ◽

Transcriptional Activation ◽

Target Gene ◽

Developmental Stages ◽

Embryonic Stem ◽

Hereditary Diseases ◽

Peroxisome Proliferator ◽

Peroxisome Proliferator Activated Receptor ◽

Stem Cell Regulation ◽

Early Developmental

The Wnt/β-catenin signaling pathway plays a crucial role in early embryonic development. Wnt/β-catenin signaling is a major regulator of cell proliferation and keeps embryonic stem cells (ESCs) in the pluripotent state. Dysregulation of Wnt signaling in the early developmental stages causes several hereditary diseases that lead to embryonic abnormalities. Several other signaling molecules are directly or indirectly activated in response to Wnt/β-catenin stimulation. The crosstalk of these signaling factors either synergizes or opposes the transcriptional activation of β-catenin/Tcf4-mediated target gene expression. Recently, the crosstalk between the peroxisome proliferator-activated receptor delta (PPARδ), which belongs to the steroid superfamily, and Wnt/β-catenin signaling has been reported to take place during several aspects of embryonic development. However, numerous questions need to be answered regarding the function and regulation of PPARδ in coordination with the Wnt/β-catenin pathway. Here, we have summarized the functional activation of the PPARδ in co-ordination with the Wnt/β-catenin pathway during the regulation of several aspects of embryonic development, stem cell regulation and maintenance, as well as during the progression of several metabolic disorders.

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Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies

Genes ◽

10.3390/genes12050638 ◽

2021 ◽

Vol 12 (5) ◽

pp. 638

Author(s):

Monika Mazur ◽

Daria Wojciechowska ◽

Ewa Sitkiewicz ◽

Agata Malinowska ◽

Bianka Świderska ◽

...

Keyword(s):

Life Cycle ◽

Developmental Stages ◽

Coupling Efficiency ◽

Slime Mold ◽

Intact Cells ◽

Life Cycle Stages ◽

Early Developmental Stages ◽

Proteomic Study ◽

And Function ◽

Early Developmental

The slime mold Dictyostelium discoideum’s life cycle includes different unicellular and multicellular stages that provide a convenient model for research concerning intracellular and intercellular mechanisms influencing mitochondria’s structure and function. We aim to determine the differences between the mitochondria isolated from the slime mold regarding its early developmental stages induced by starvation, namely the unicellular (U), aggregation (A) and streams (S) stages, at the bioenergetic and proteome levels. We measured the oxygen consumption of intact cells using the Clarke electrode and observed a distinct decrease in mitochondrial coupling capacity for stage S cells and a decrease in mitochondrial coupling efficiency for stage A and S cells. We also found changes in spare respiratory capacity. We performed a wide comparative proteomic study. During the transition from the unicellular stage to the multicellular stage, important proteomic differences occurred in stages A and S relating to the proteins of the main mitochondrial functional groups, showing characteristic tendencies that could be associated with their ongoing adaptation to starvation following cell reprogramming during the switch to gluconeogenesis. We suggest that the main mitochondrial processes are downregulated during the early developmental stages, although this needs to be verified by extending analogous studies to the next slime mold life cycle stages.

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