Identification of Specific Protein Markers that Correlate with Initiation and Progression of Microbially Influenced Corrosion

1992 ◽  
Author(s):  
Victoria G. Prevatt
Keyword(s):  
Specific Protein ◽  
Protein Markers ◽  
Microbially Influenced Corrosion

scholarly journals Anti-Stress, Glial- and Neuro-Differentiation Potential of Resveratrol: Characterization by Cellular, Biochemical and Imaging Assays

Nutrients ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 671
Author(s):  
Sajal Afzal ◽  
Sukant Garg ◽  
Divya Adiga ◽  
Yoshiyuki Ishida ◽  
Keiji Terao ◽  
...  
Keyword(s):  
Oxidative Dna Damage ◽  
Specific Protein ◽  
New Drugs ◽  
Protein Markers ◽  
Human Neuroblastoma ◽  
Differentiation Potential ◽  
Daily Lives ◽  
Rat Glioma ◽  
Age Related

Environmental stress, exhaustive industrialization and the use of chemicals in our daily lives contribute to increasing incidence of cancer and other pathologies. Although the cancer treatment has revolutionized in last 2–3 decades, shortcomings such as (i) extremely high cost of treatment, (ii) poor availability of drugs, (iii) severe side effects and (iv) emergence of drug resistance have prioritized the need of developing alternate natural, economic and welfare (NEW) therapeutics reagents. Identification and characterization of such anti-stress NEW drugs that not only limit the growth of cancer cells but also reprogram them to perform their specific functions are highly desired. We recruited rat glioma- and human neuroblastoma-based assays to explore such activities of resveratrol, a naturally occurring stilbenoid. We demonstrate that nontoxic doses of resveratrol protect cells against a variety of stresses that are largely involved in age-related brain pathologies. These included oxidative, DNA damage, metal toxicity, heat, hypoxia, and protein aggregation stresses. Furthermore, it caused differentiation of cells to functional astrocytes and neurons as characterized by the upregulation of their specific protein markers. These findings endorse multiple bioactivities of resveratrol and encourage them to be tested for their benefits in animal models and humans.

scholarly journals PTSD Susceptibility and Challenges: Pathophysiological Consequences of Behavioral Symptoms

2020 ◽  
Vol 185 (Supplement_1) ◽  
pp. 279-285
Author(s):  
Mulugu V Brahmajothi ◽  
Mohamed B Abou-Donia
Keyword(s):  
Calcium Binding ◽  
Military Service ◽  
The United States ◽  
Specific Protein ◽  
Brain Parenchyma ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Markers ◽  
Necrosis Factor Alpha ◽  
Magnetic Resonance Imaging Mri ◽  
Subclinical Symptoms

Abstract Introduction Posttraumatic stress disorder (PTSD) can develop during the aftermath of traumatic events. Although many are impacted by several stressors, nearly 3.6% suffer from PTSD in the United States with higher incidence reported in military service personnel. Any injury to the blood-brain barrier can ignite an array of biological signaling molecules in the immune-privileged brain parenchyma, which can disrupt the synaptic neural network, resulting in altered behavior. Materials and Methods In this preliminary study, we compared 20 PTSD veterans with age-matched healthy veterans to identify plasma levels of brain-specific protein markers using enzyme-linked immunosorbent assay/immunofluorometric sandwich assay for neurotrophic factors and neuropoietic cytokines, and catalytic activity of matrix metalloproteinase (MMP) by zymography. Results We observed an increased level of glial fibrillary acidic protein, tumor necrosis factor-alpha, interleukin 6, and MMP2 and MMP9 but decreased level of brain-derived neurotrophic factor, nerve growth factor-beta, and negligible difference in astroglial marker S100 calcium-binding protein B compared to controls. Conclusion Identification of neural biomarkers is essential to understand the subclinical symptoms for the diagnosis PTSD, which may not be visible by magnetic resonance imaging (MRI/fMRI) and may take years to clinically manifest.

scholarly journals Differential tissue-specific protein markers of vaginal carcinoma

2009 ◽  
Vol 100 (8) ◽  
pp. 1303-1314 ◽  
Author(s):  
K Hellman ◽  
A A Alaiya ◽  
S Becker ◽  
M Lomnytska ◽  
K Schedvins ◽  
...  
Keyword(s):  
Specific Protein ◽  
Protein Markers ◽  
Tissue Specific ◽  
Vaginal Carcinoma ◽  
Differential Tissue

scholarly journals Proteomic similarity of the Littorinid snails in the evolutionary context

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8546 ◽  
Author(s):  
Arina L. Maltseva ◽  
Marina A. Varfolomeeva ◽  
Arseniy A. Lobov ◽  
Polina Tikanova ◽  
Marina Panova ◽  
...  
Keyword(s):  
Phylogenetic Tree ◽  
Evolutionary History ◽  
Geographic Location ◽  
Body Part ◽  
Specific Protein ◽  
Protein Markers ◽  
Cophenetic Correlation ◽  
Additional Information ◽  
History Of ◽  
Using Data

Background The introduction of DNA-based molecular markers made a revolution in biological systematics. However, in cases of very recent divergence events, the neutral divergence may be too slow, and the analysis of adaptive part of the genome is more informative to reconstruct the recent evolutionary history of young species. The advantage of proteomics is its ability to reflect the biochemical machinery of life. It may help both to identify rapidly evolving genes and to interpret their functions. Methods Here we applied a comparative gel-based proteomic analysis to several species from the gastropod family Littorinidae. Proteomes were clustered to assess differences related to species, geographic location, sex and body part, using data on presence/absence of proteins in samples and data on protein occurrence frequency in samples of different species. Cluster support was assessed using multiscale bootstrap resampling and the stability of clustering—using cluster-wise index of cluster stability. Taxon-specific protein markers were derived using IndVal method. Proteomic trees were compared to consensus phylogenetic tree (based on neutral genetic markers) using estimates of the Robinson–Foulds distance, the Fowlkes–Mallows index and cophenetic correlation. Results Overall, the DNA-based phylogenetic tree and the proteomic similarity tree had consistent topologies. Further, we observed some interesting deviations of the proteomic littorinid tree from the neutral expectations. (1) There were signs of molecular parallelism in two Littoraria species that phylogenetically are quite distant, but live in similar habitats. (2) Proteome divergence was unexpectedly high between very closely related Littorina fabalis and L. obtusata, possibly reflecting their ecology-driven divergence. (3) Conservative house-keeping proteins were usually identified as markers for cryptic species groups (“saxatilis” and “obtusata” groups in the Littorina genus) and for genera (Littoraria and Echinolittorina species pairs), while metabolic enzymes and stress-related proteins (both potentially adaptively important) were often identified as markers supporting species branches. (4) In all five Littorina species British populations were separated from the European mainland populations, possibly reflecting their recent phylogeographic history. Altogether our study shows that proteomic data, when interpreted in the context of DNA-based phylogeny, can bring additional information on the evolutionary history of species.

scholarly journals CImP: Cellular Imprinting Proteomics applied to ocular disorders elicited by Congenital Zika virus Syndrome

2019 ◽  
Author(s):  
Livia Rosa-Fernandes ◽  
Raquel Hora Barbosa ◽  
Maria Luiza B. dos Santos ◽  
Claudia B. Angeli ◽  
Thiago P. Silva ◽  
...  
Keyword(s):  
Zika Virus ◽  
Ocular Surface ◽  
Molecular Mechanisms ◽  
Membrane Filter ◽  
Neurological Diseases ◽  
Specific Protein ◽  
Protein Markers ◽  
Impression Cytology ◽  
Zikv Infection ◽  
Molecular Alterations

AbstractIMPORTANCEOcular complications in infants with Congenital Zika Syndrome (CZS) have been reported. However, the molecular mechanisms underlying of eye dysfunctions are presently unknown.OBJECTIVEA method (termed Cellular Imprinting Proteomics, CImP) for the identification and quantification of the ocular surface proteome using a minimally invasive membrane filter device is described. Moreover, The CImP method was applied to profile the molecular alterations in the eyes of infants exposed to Zika virus (ZIKV) infection during gestation.DESIGN, SETTINGS AND PARTICIMPANTSThe CImP method was applied to a cohort divided into three conditions: 1) Ctrl (infants with no infectious diseases, n=5). 2) Zikv (infants exposed to ZIKV gestation, with no microcephaly, n=5). 3) ZikvCZS(infants exposed to ZIKV, with microcephaly, n=3). All conditions were age and sex-matched. An improved impression cytology method was used to capture the outermost ocular surface cells. The number of impression cytology membrane collected was: Ctrl (12), Zikv (14) and ZikvCZS(8). Proteins were extracted and analysed using mass spectrometry-based proteomics technology followed by statistical analysis. Parallel reaction monitoring was performed to validate the expression of specific protein markers.RESULTSUsing the CImP method, 2209 proteins were identified on the membrane-captured conjunctiva epithelial cells. Modulation of neutrophil degranulation, cell death, ocular and neurodevelopment pathways are reported in infants with CZS compared to matched controls. Moreover, the molecular pattern of ocular surface cells retrieved from infants infected during the gestation but with no CZS was different from matched controls.CONCLUSIONS AND PERSPECTIVESMolecular alterations in the ocular cell surface associated to ZIKV infection with and without CZS complications are reported for the first time. We predict that this method will be introduced successfully in the study of several neurological diseases with the aim to identify novel diagnostic and therapeutic biomarkers.

scholarly journals Identification of Novel Biomarker Candidates for Hypertrophic Cardiomyopathy and Other Cardiovascular Diseases Leading to Heart Failure

2016 ◽  
pp. 751-762 ◽  
Author(s):  
H. REHULKOVA ◽  
P. REHULKA ◽  
A. MYSLIVCOVA FUCIKOVA ◽  
J. STULIK ◽  
R. PUDIL
Keyword(s):  
Hypertrophic Cardiomyopathy ◽  
Cardiovascular Diseases ◽  
Stable Coronary Artery Disease ◽  
Specific Protein ◽  
Protein Markers ◽  
Protein Biomarkers ◽  
Absolute Quantitation ◽  
Obstructive Hypertrophic Cardiomyopathy ◽  
Isobaric Tag ◽  
Cardiovascular Pathologies

In-depth proteome discovery analysis represents new strategy in an effort to identify novel reliable specific protein markers for hypertrophic cardiomyopathy and other life threatening cardiovascular diseases. To systematically identify novel protein biomarkers of cardiovascular diseases with high mortality we employed an isobaric tag for relative and absolute quantitation (iTRAQ) proteome technology to make comparative analysis of plasma samples obtained from patients suffering from non-obstructive hypertrophic cardiomyopathy, stable dilated cardiomyopathy, aortic valve stenosis, chronic stable coronary artery disease and stable arterial hypertension. We found 128 plasma proteins whose abundances were uniquely regulated among the analyzed cardiovascular pathologies. 49 of them have not been described yet. Additionally, application of statistical exploratory analyses of the measured protein profiles indicated the relationship in pathophysiology of the examined cardiovascular pathologies.

scholarly journals Proteomic analysis of cerebrospinal fluid discriminates malignant and nonmalignant disease of the central nervous system and identifies specific protein markers

PROTEOMICS ◽  
2006 ◽  
Vol 6 (23) ◽  
pp. 6277-6287 ◽  
Author(s):  
Fatima W. Khwaja ◽  
John David Larkin Nolen ◽  
Savaas E. Mendrinos ◽  
Melinda M. Lewis ◽  
Jeffrey J. Olson ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Proteomic Analysis ◽  
Specific Protein ◽  
Protein Markers ◽  
Nonmalignant Disease ◽  
The Central Nervous System

scholarly journals PU.1 drives specification of pluripotent stem cell-derived endothelial cells to LSEC-like cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jonathan De Smedt ◽  
Elise Anne van Os ◽  
Irene Talon ◽  
Sreya Ghosh ◽  
Burak Toprakhisar ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cell Lineage ◽  
Specific Protein ◽  
Immune Functions ◽  
Protein Markers ◽  
Liver Sinusoidal Endothelial Cells ◽  
Lineage Specification

AbstractTo date, there is no representative in vitro model for liver sinusoidal endothelial cells (LSECs), as primary LSECs dedifferentiate very fast in culture and no combination of cytokines or growth factors can induce an LSEC fate in (pluripotent stem cell (PSC)-derived) endothelial cells (ECs). Furthermore, the transcriptional programmes driving an LSEC fate have not yet been described. Here, we first present a computational workflow (CenTFinder) that can identify transcription factors (TFs) that are crucial for modulating pathways involved in cell lineage specification. Using CenTFinder, we identified several novel LSEC-specific protein markers, such as FCN2 and FCN3, which were validated by analysis of previously published single-cell RNAseq data. We also identified PU.1 (encoded by the SPI1 gene) as a major regulator of LSEC-specific immune functions. We show that SPI1 overexpression (combined with the general EC TF ETV2) in human PSCs induces ECs with an LSEC-like phenotype. The ETV2-SPI1-ECs display increased expression of LSEC markers, such as CD32B and MRC1, as well as several of the proposed novel markers. More importantly, ETV2-SPI1-ECs acquire LSEC functions, including uptake of FSA-FITC, as well as labelled IgG. In conclusion, we present the CenTFinder computational tool to identify key regulatory TFs within specific pathways, in this work pathways of lineage specification, and we demonstrate its use by the identification and validation of PU.1 as a master regulator for LSEC fating.

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