scholarly journals USE OF A DOUBLE PHASE (LIQUID OVERLAY) CULTURE SYSTEM TO ENHANCE SHOOT QUALITY AND ROOTING RESPONSE IN MINIATURE ROSES

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 581c-581
Author(s):  
S.M. Eichorst ◽  
R.B. Rogers ◽  
M.A.L. Smith
Keyword(s):  
Phase System ◽  
Liquid Media ◽  
Media Control ◽  
System A ◽  
Solid Media ◽  
Double Phase ◽  
Phase Liquid ◽  
Length And Area ◽  
Rooting Response ◽  
Shoot Quality

Use of a liquid media during micropropagation has promoted improved proliferation and rooting response in several species. In this experiment, a double phase system (a combination of liquid and agar solidified medium) was applied to three cultivars of miniature roses (Rosa chinensis var. minima) to determine the effects on shoot quality and subsequent ex-vitro rooting. Applications of liquid media to the surface of agar solidified media were made at 0, 2, and 4 weeks. Evaluation via computerized image analysis after eight weeks of proliferation revealed equal or greater values for shoot length, area and weighted density (equivalent to fresh weight) for cultures receiving overlay, regardless of timing, compared to the solid media control. Additionally, application of a liquid overlay improved rooting response by up to 20% over the control and resulted in a tendency for a greater number of roots of greater length and area than the treatment without liquid media overlay.

Application of Ionic Liquids for the Determination of Lipophilicity Parameters Using TLC Method, and QSRR Analysis for the Antipsychotic Drugs

2020 ◽  
Vol 16 (7) ◽  
pp. 848-859
Author(s):  
Dominik Mieszkowski ◽  
Marcin Koba ◽  
Michał P. Marszałł
Keyword(s):  
Ionic Liquids ◽  
Antipsychotic Drugs ◽  
Molecular Descriptors ◽  
Harmful Effect ◽  
Correlation Coefficients ◽  
Reversed Phase ◽  
Assessment Process ◽  
Phase System ◽  
Phase Liquid ◽  
Lipophilicity Parameters

Background: Reversed-phase liquid chromatography may cause difficulties, especially in the case of basic drugs due to the strong silanophilic interactions in the partition mechanism. Recently, imidazolium-based ionic liquids additives appeared interesting and a convenient solution for suppressing the harmful effect of free residuals of silanol groups, allowing remodeling of the stationary/mobile-phase system, and thus improving the lipophilicity assessment process. Objective: The aim of the study was to evaluate the retention behavior of basic antipsychotics using various RP-LC systems, and compare them with data obtained from the modified ionic-liquids RP-TLC systems, and perform the QSRR analysis. Methods: Retention and lipophilicity parameters of diverse antipsychotics have been examined in various RP-LC systems. Lipophilicity indices were compared with miscellaneous computed logP values. Furthermore, a large number of molecular descriptors have been computed and compared using various medicinal chemistry software, in order to contribute to the analysis of QSRR. Results: Designated correlation coefficients showed that lipophilicity parameters from TLC systems without [EMIM][BF4] additive correlates very poor with the calculated logPs indices, whereas the indices from the traditional HPLC and TLC systems (with [EMIM][BF4]) were clearly better. Furthermore, QSRR analysis performed for these experimentally obtained lipophilicity parameters showed significant relationships between the retention constants (RO>M, logkw) and the in silico calculated physicochemical molecular descriptors. Conclusion: ILs additive may be a significant factor affecting the lipophilicity of basic compounds, thus their use may be favorable in lipophilicity assessment studies. QSRR models with ILs showed that they may be useful in searching/or predicting HPLC/TLC retention parameters for the new/other antipsychotic drugs.

Recovery of Humic-Reducing Bacteria from a Diversity of Environments

1998 ◽  
Vol 64 (4) ◽  
pp. 1504-1509 ◽  
Author(s):  
John D. Coates ◽  
Debra J. Ellis ◽  
Elizabeth L. Blunt-Harris ◽  
Catherine V. Gaw ◽  
Eric E. Roden ◽  
...  
Keyword(s):  
Humic Substance ◽  
Electron Acceptor ◽  
Microbial Reduction ◽  
Acetate Oxidation ◽  
Liquid Media ◽  
Sedimentary Environments ◽  
Sediment Types ◽  
Solid Media ◽  
The Family ◽  
Reducing Bacteria

ABSTRACT To evaluate which microorganisms might be responsible for microbial reduction of humic substances in sedimentary environments, humic-reducing bacteria were isolated from a variety of sediment types. These included lake sediments, pristine and contaminated wetland sediments, and marine sediments. In each of the sediment types, all of the humic reducers recovered with acetate as the electron donor and the humic substance analog, 2,6-anthraquinone disulfonate (AQDS), as the electron acceptor were members of the familyGeobacteraceae. This was true whether the AQDS-reducing bacteria were enriched prior to isolation on solid media or were recovered from the highest positive dilutions of sediments in liquid media. All of the isolates tested not only conserved energy to support growth from acetate oxidation coupled to AQDS reduction but also could oxidize acetate with highly purified soil humic acids as the sole electron acceptor. All of the isolates tested were also able to grow with Fe(III) serving as the sole electron acceptor. This is consistent with previous studies that have suggested that the capacity for Fe(III) reduction is a common feature of all members of theGeobacteraceae. These studies demonstrate that the potential for microbial humic substance reduction can be found in a wide variety of sediment types and suggest thatGeobacteraceae species might be important humic-reducing organisms in sediments.

Culture of Helicobacter pylori from domestic water samples – the impact of strain variation on growth on solid and in liquid media

2006 ◽  
Vol 54 (3) ◽  
pp. 147-152 ◽  
Author(s):  
R.J. Owen ◽  
S.A. Chisholm ◽  
G. Brick ◽  
J.V. Lee ◽  
S. Surman-Lee ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Culture Media ◽  
Tap Water ◽  
Clinical Isolates ◽  
Strain Variation ◽  
Liquid Media ◽  
Domestic Water ◽  
Source Of Infection ◽  
Solid Media ◽  
The Impact

Helicobacter pylori is an important global human pathogen and there is growing evidence from PCR assays that contaminated drinking water might be a possible source of infection in some circumstances. There are no validated protocols for direct isolation but various culture media have been developed for possible environmental sampling. Our aim here was to investigate how inter-strain variation might affect the interpretation of results with such media. Two laboratory adapted reference strains and four recent clinical isolates were tested on four solid media and in ten liquid media. Considerable variation was found between strains in their ability to recover on the different media after stress exposure (suspension in sterile tap water). Generally, clinical isolates were less robust than the laboratory-adapted strains and, overall, the former required longer recovery times. Our findings highlighted the importance of using a range of isolates for evaluations, as examination of laboratory-adapted strains alone did not provide an accurate representation of the utility of media that may be used to recover H. pylori from water.

Design and Simulation of Booster's Controller Based on Matlab

2014 ◽  
Vol 672-674 ◽  
pp. 888-893
Author(s):  
Xiao Long Xiao ◽  
Xiao Hua Ding ◽  
Hao Zhang
Keyword(s):  
Circuit Simulation ◽  
Dynamic Performance ◽  
Phase System ◽  
Pi Regulator ◽  
Small Signal Analysis ◽  
System A ◽  
Working Principle ◽  
Type Switch ◽  
And Control ◽  
The Mathematical Model

According to the working principle of the Boost type switch converter, the small signal analysis method was used to model the Boost circuit. Its transfer function exists right half plane zero. The system is a non-minimum phase system. A PI regulator was designed. The boost circuit simulation model was built through matlab. The simulation result has a good static and dynamic performance. It verifies the rationality of the mathematical model and control strategy. It can conduct the actual circuit design.

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