scholarly journals 175 GENETIC TRANSFORMATION OF COFFEE (COFFEA ARABICA L.) BY AGROBACTERIUM SPP.

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 454a-454 ◽  
Author(s):  
A. Virginia Freire ◽  
David A. Lightfoot ◽  
John E. Preece
Keyword(s):  
Genetic Transformation ◽  
Hairy Roots ◽  
Generation Time ◽  
Coffea Arabica ◽  
Pcr Amplification ◽  
Dna Encoding ◽  
Ipt Gene ◽  
Coffea Arabica L ◽  
Coffee Breeding ◽  
Efficient Genetic Transformation

Efficient genetic transformation could enhance coffee breeding, which is limited by its long generation time and narrow genetic base. Three explant types of three coffee cultivars were inoculated with 14 strains of Agrobacterium spp. Callus and hairy roots were produced with 13 of the 14 strains tested. With A. tumefaciens, nopaline strains were more effective than octopine strains. Cucumopine and mannopine strains of A. rhizogenes were both effective in inducing hairy roots and callus. PCR amplification of a 0.72 Kb fragment of T-DNA encoding a portion of the ipt gene was achieved with DNA from A. tumefaciens strain A208 and with putatively transformed tissue inoculated with A208. No amplification was observed with virB in putatively transformed tissue which indicates it was not contaminated with Agrobacterium. We conclude that coffee can be genetically transformed by some Agrobacterium strains.

scholarly journals Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures

2011 ◽  
Vol 11 (1) ◽  
pp. 92 ◽  
Author(s):  
Alessandra F Ribas ◽  
Eveline Dechamp ◽  
Anthony Champion ◽  
Benoît Bertrand ◽  
Marie-Christine Combes ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Embryogenic Callus ◽  
Coffea Arabica ◽  
Callus Cultures ◽  
Coffea Arabica L

scholarly journals AGROBACTERIUM-MEDIATED TRANSFORMATION OF PANAX VIETNAMENSIS HA ET GRUSHV.

2018 ◽  
Vol 15 (4) ◽  
pp. 641-650
Author(s):  
Duong Tan Nhut ◽  
Nguyen Phuc Huy ◽  
Trinh Thi Huong ◽  
Vu Quoc Luan ◽  
Vu Thi Hien ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Genetic Transformation ◽  
Hairy Root ◽  
Hairy Roots ◽  
Large Scale ◽  
Continuous Production ◽  
Pcr Amplification ◽  
Limited Area ◽  
Root Induction

In recent years, the Agrobacterium-mediated genetic transformation system has become the most useful method widely used for the introduction of foreign genes into plant cells followed by regeneration of genetically improved plants. Panax vietnamensis Ha et Grushv. is a highly valued medicinal plant native to Vietnam with limited area of distribution. This report illustrates the possibilities of biotechnology for genetic transformation aimed at establishing an effective production of secondary metabolites in P. vietnamensis. In the present investigation, 0.5 cm2 leaf blades, 1 cm long leaf petioles and 0.5 cm3 callus clusters were used for the hairy root induction. Results indicated that hairy roots were induced on P. vietnamensis callus clusters co-cultivated with Agrobacterium rhizogenes strain ATCC15834 at OD600 of 0.5 with an infection time of 20 min and a supplementation of 100 mM acetosyringone. PCR amplification of the DNA isolated from the resulting hairy roots was used to confirm the presence of rol genes. Compared to in vitro rhizome cultures, hairy root cultures appear to be potential for continuous production of valuable secondary metabolites with similar saponin profiles. The protocol described in this study is simple and rapid and therefore, can be used for large-scale experiments for the rapid production of valuable compounds.

SISTEMA DE PRODUCCIÓN DE CAFÉ (Coffea arabica L.) EN LA COMUNIDAD DEL CERRO CUATE, ILIATENCO, GUERRERO

2018 ◽  
Vol 11 (10) ◽  
Author(s):  
A. Delgado-Alvarado
Keyword(s):  
Coffea Arabica ◽  
El Sistema ◽  
Coffea Arabica L

Objetivo: Analizar el proceso de producción y comercialización de café en la comunidad del Cerro Cuate, Iliatenco, en la región de la Montaña de Guerrero, México.Diseño/Metodología/aproximación: el trabajo se realizó por; 1) investigación documental), 2) selección del área de estudio, 3) Entrevista estructurada con preguntas abiertas a 22 cafeticultores, soportada con la técnica de encuesta seccional y la herramienta de cédulas de entrevistas, y 4) análisis de la información. El tamaño de muestra se definió por el método de muestreo por conveniencia, y la selección de las unidades de análisis por la técnica bola de nieve. Resultados: El sistema de producción de café que predominó fue el sistema rústico de montaña, intercalado con plátano y frutales. La máxima productividad de las plantaciones se alcanza de 5.5 a 7.5años de edad, la renovación de plantas la hacen a los 12 años. El rendimiento de café fue de 3.7 kg por planta por año. La producción del café la realizan principalmente hombres (77.3%), entre 56 y 70años de edad. La venta se realiza en la presentación de café capulín a granel a intermediarios, a la ARIC, a CAFECO, a la Unión de Ejidos y a la Organización Mixtrui.Limitaciones del estudio/implicaciones: Se da una propuesta de mejora para favorecer el proceso de producción, beneficio y comercialización del café.Hallazgos/conclusiones: Coffea arabica es el principal café que se cultiva, con las variedades Typica, Caturra, Mundo Novo, Garnica y Bourbón. Los factores que limitan su producción y calidad son faltade planeación en manejo del cultivo y no contar con asesoría técnica.

Deposição da calda de pulverização em diferentes volumes vegetativos de Coffea arabica L.

2017 ◽  
Vol 12 (1) ◽  
pp. 69 ◽  
Author(s):  
Felipe Santinato ◽  
Renato Adriane Alves Ruas ◽  
Carlos Diego Silva ◽  
Rouverson Pereira Da Silva ◽  
Victor Afonso Reis Gonçalves ◽  
...  
Keyword(s):  
Coffea Arabica ◽  
Para Determinar ◽  
Coffea Arabica L

A disposição dos ramos e sobreposição das folhas em plantas de café dificultam a penetração da calda pulverizada. Portanto, para determinar o volume de calda adequado, é importante verificar o estado de enfolhamento da lavoura antes da aplicação. Objetivou-se com este trabalho, avaliar a deposição de caldas de pulverização em lavouras de café aplicadas em diferentes volumes vegetativos. Os tratamentos foram dispostos seguindo esquema de parcelas subdivididas em cada volume vegetativo (5.000; 7.500; 10.000 e 17.500 m<sup>3</sup> ha<sup>-1</sup>). Sendo as parcelas cinco volumes de calda (150, 300, 450, 600 e 750 L ha<sup>-1</sup>) e as subparcelas três posições no dossel do cafeeiro (terço superior, médio e inferior) com quatro repetições. Nos quatro volumes vegetativos estudados, não ocorreu interação significativa (p&gt;0,05) entre o volume aplicado e os diferentes volumes vegetativos. Porém, os volumes vegetativos de 5.000,0 e 17.500,0 m<sup>3</sup> ha<sup>-1</sup> a deposição foi crescente com aumento das vazões, o que pode ser atribuído a densidade foliar. Não foi verificada diferença estatística (p&gt; 0,05), entre as médias de deposição nos diferentes terços (alturas) no dossel das plantas de café, nos volumes vegetativos 5.000,0 m<sup>3 </sup>ha<sup>-1</sup>, 10.000,0 m<sup>3 </sup>ha<sup>-1</sup> e 17.500,0 m<sup>3 </sup>ha<sup>-1</sup>. Contudo, no volume de 7.500,0 m<sup>3 </sup>ha<sup>-1</sup> houve maior deposição no terço mediano em relação ao terço inferior. A deposição é maior à medida que aumenta o volume de calda aplicado. Em plantas com menor densidade foliar, há incremento na deposição. A deposição é maior no terço mediano em relação ao terço inferior.

Pengelolaan Pemangkasan Tanaman Kopi Arabika (Coffea arabica L.) di Kebun Blawan, Bondowoso, Jawa Timur

2016 ◽  
Vol 4 (3) ◽  
pp. 266
Author(s):  
Vinsensia Febrina Sianturi ◽  
Ade Wachjar
Keyword(s):  
Coffea Arabica ◽  
Coffea Arabica L

<p><em>Kopi merupakan salah satu komoditas perkebunan yang memiliki nilai ekonomi yang cukup tinggi</em><em>. </em><em>Kegiatan magang bertujuan mempelajari teknik budidaya</em><em> </em><em>tanaman</em><em> </em><em>dan</em><em> p</em><em>engelolaan</em><em> </em><em>perkebunan kopi, mempelajari dan menganalisis permasalahan yang dihadapi di lapangan mengenai pengelolaan pemangkasan serta solusi mengatasinya.</em><em> P</em><em>emangkasan </em><em>bertujuan </em><em>agar pohon tetap rendah sehingga mudah perawatannya, </em><em>dan </em><em>membentuk cabang-cabang produksi yang baru.</em><em> </em><em>Kegiatan magang dilaksanakan di Kebun Blawan, Bondowoso, Jawa Timur</em><em>,</em><em> mulai bulan Februari sampai dengan Juni 2014.</em><em> </em><em>Pengumpulan data </em><em>primer diperoleh melalui </em><em>pengamatan dan praktik kerja secara langsung meliputi kegiatan pemeliharaan tanaman yaitu pemangkasan lepas panen (pengamatan cabang-cabang tanaman, tinggi tanaman, jumlah tunas yang tumbuh)</em><em>, sedangkan data sekunder </em><em>diperoleh melalui </em><em>laporan manajemen perusahaan. </em><em>A</em><em>nalisis </em><em>data </em><em>yang dilakukan secara</em><em> deskriptif, rata-rata dan persentase</em><em>. Pemangkasan yang dilakukan</em><em> </em><em>termasuk dalam kategori pemangkasan ringan.</em><em> Tanaman kopi </em><em>yang memiliki kondisi cabang yang merata dan seimbang sangat mempengaruhi </em><em>hasil </em><em>taksasi</em><em> produksi</em><em>.</em><em> Banyak cabang harus dipangkas karena cabang-cabang yang sudah tua dan terserang penyakit. Setelah melakukan pemangkasan, tanaman menghasilkan tunas-tunas baru</em><em>.</em><em></em></p>

scholarly journals Unveiling a unique genetic diversity of cultivated Coffea arabica L. in its main domestication center: Yemen

2021 ◽  
Author(s):  
C. Montagnon ◽  
A. Mahyoub ◽  
W. Solano ◽  
F. Sheibani
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Coffea Arabica ◽  
Genetic Improvement ◽  
The World ◽  
Genetic Clusters ◽  
Domestication Process ◽  
Almost All ◽  
Coffea Arabica L ◽  
Cup Quality

AbstractWhilst it is established that almost all cultivated coffee (Coffea arabica L.) varieties originated in Yemen after some coffee seeds were introduced into Yemen from neighboring Ethiopia, the actual coffee genetic diversity in Yemen and its significance to the coffee world had never been explored. We observed five genetic clusters. The first cluster, which we named the Ethiopian-Only (EO) cluster, was made up exclusively of the Ethiopian accessions. This cluster was clearly separated from the Yemen and cultivated varieties clusters, hence confirming the genetic distance between wild Ethiopian accessions and coffee cultivated varieties around the world. The second cluster, which we named the SL-17 cluster, was a small cluster of cultivated worldwide varieties and included no Yemen samples. Two other clusters were made up of worldwide varieties and Yemen samples. We named these the Yemen Typica-Bourbon cluster and the Yemen SL-34 cluster. Finally, we observed one cluster that was unique to Yemen and was not related to any known cultivated varieties and not even to any known Ethiopian accession: we name this cluster the New-Yemen cluster. We discuss the consequences of these findings and their potential to pave the way for further comprehensive genetic improvement projects for the identification of major resilience/adaptation and cup quality genes that have been shaped through the domestication process of C. arabica.

Multiscale analyses of growth and berry distributions along four branching orders and vertical profile of Coffea arabica L. cultivated under high-density planting systems

2021 ◽  
Vol 281 ◽  
pp. 109934
Author(s):  
Miroslava Rakocevic ◽  
Fabio Takeshi Matsunaga ◽  
Danilo Força Baroni ◽  
Eliemar Campostrini ◽  
Evelyne Costes
Keyword(s):  
Vertical Profile ◽  
Coffea Arabica ◽  
High Density ◽  
Coffea Arabica L
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