scholarly journals Determination of Optimum Nitrogen Ratios and Concentrations for Embryo Cultures of Alstroemeria

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 630e-630
Author(s):  
Paula C. Moreck ◽  
Mark Bridgen
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Total Nitrogen ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Ms Medium ◽  
Embryo Cultures ◽  
The Media

The rates of in ovulo germination of embryos of three genotypes of Alstroemeria were observed. Ovules were harvested ten days after pollination and cultured on sixteen different treatments containing Murashige and Skoog (MS) basal medium with no plant growth regulators. The media contained four different concentrations of total nitrogen: 20, 40, 60, 80 mm. Within these concentrations were either 1:0, 1:1, 1:2, or 2:1 ratios of nitrate to ammonium. Standard MS medium, with a concentration of 60 mm total nitrogen and a ratio of 2:1 nitrate to ammonium, was used as the control. The overall rates of germination for all three genotypes were low in all treatments. The percentage of zygotic germination was low while the percentage of somatic embryos produced was high. In some situations, callus or deformed embryos were produced. Effects of the treatments on embryo development and germination will be discussed.

scholarly journals EFFECT OF PLANT GROWTH REGULATORS ON SOMATIC EMBRYOGENESIS OF EGGPLANT (SOLANUM MELONGENA L.)

2009 ◽  
Vol 12 (17) ◽  
pp. 71-80
Author(s):  
Nam Ngoc Trinh ◽  
Sanh Du Nguyen
Keyword(s):  
Somatic Embryogenesis ◽  
Survival Rate ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Somatic Embryos ◽  
Solanum Melongena ◽  
Ms Medium

On the MS medium containing only 2,4-D, callus induction was inhibited. Moreover, these calli were friable and turned brown after two weeks of culture. the three-week old calli were then transferred to the MS medium containing NAA 0.5 mg/l and kinetin 1 mg/l. The somatic embryos with globular shape appeared after 10 days of culture, while the heart shape, torpedo_shape and cotyledonary_shape embryos appeared successively after 15 days of culture. The abnormal embryos occupied at a rate of 34.3% and rarely germinated to plantlets. On the MS medium without plant growth regulators or only with NAA, somatic embryos could not be induced. On MS medium supplemented with ethephon 3 mg/l somatic embryogenesis from calli was inhibited. Plantlets derived from the eggplant somatic embryos had a survival rate up to 95% when transferred to the pots.

Somatic Embryogenesis and Plant Development in Eight Cultivars of Pecan

HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 573-576 ◽  
Author(s):  
I.E. Yates ◽  
C.C. Reilly
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Plant Development ◽  
Fruit Development ◽  
Somatic Embryos ◽  
Carya Illinoensis ◽  
Appreciable Variation

The influence of stage of fruit development and plant growth regulators on somatic embryogenesis and the relation of cultivar response on somatic embryogenesis and subsequent plant development have been investigated in eight cultivars of pecan [Carya illinoensis (Wangenh.) C. Koch]. Explants from the micropylar region of the ovule were more embryogenic when removed from fruits in the liquid endosperm stage than were intact ovules from less-mature fruits or from cotyledonary segments of more-mature fruits. Explants conditioned on medium containing auxin alone or auxin + cytokinin produced more somatic embryos than medium containing cytokinin alone. Under the conditions of this study, frequency of embryogenesis, as well as the germination of somatic embryos leading to plant development, indicated appreciable variation among cultivars. Plant development was greatest by far from somatic embryos of `Schley' than other cultivars studied.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

scholarly journals In vitro organogenesis and plant regeneration of Passiflora xishuangbannaensis, a species with extremely small populations

2021 ◽  
Author(s):  
Yuan-yuan Meng ◽  
Shi-jie Song ◽  
Sven Landrein
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Basal Medium ◽  
Ex Situ ◽  
Naphthaleneacetic Acid ◽  
South American ◽  
South American Species

Abstract Passiflora xishuangbannaensis (Passifloraceae) is endemic to a few sites of Mengyang nature reserve in Yunnan, Xishuangbanna and less than 40 individuals have been recorded. Nine Passiflora species are endemic to Yunnan with most species occurring in South America, making P. xishuangbannaensis highly significant and emblematic to the conservation work in the region. This study is designed to provide the first protocol for in vitro organogenesis and plant regeneration for ex situ conservation and reintroduction for an Asian Passiflora species. Using internodes, petioles and tendrils we optimize calli formation and root elongation using several plant growth regulators, individually or in combination. We also assess the genetic stability of regenerated cells. The maximum callus induction and shoot bud differentiation were both achieved on half Murashige and Skoog basal medium supplemented with 4.44 µM 6-Benzylaminopurine and 1.08 µM 1-Naphthaleneacetic acid. The best rooting was achieved from 30 days old, regenerated shoots on half Murashige and Skoog basal medium supplemented with 1.08 µM 1-Naphthaleneacetic acid. Micropropagated plants were subjected to inter simple sequence repeat markers analyses. Collectively, 86 bands were generated from 6 primers of which 12 bands were polymorphic, showing genetic variation between the regenerated plantlets and the original plant. Response to plant growth regulators was more specific than most other studies using South American species, which could be explained by the morphological and physiological differences between South American and Asian Passiflora species

The flush development of evergreen recurrent flushing perennials: an experimental overview with insights into the flowering of olive

2020 ◽  
Vol 68 (5) ◽  
pp. 345
Author(s):  
Trevor Olesen ◽  
Michelle Wirthensohn
Keyword(s):  
Water Stress ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Development ◽  
New Work ◽  
Temperature Dependent ◽  
Final Size ◽  
Stimulation Of

Recurrent flushing perennials are those that grow by episodic waves of shoot extension under conditions continuously favourable for growth. Here we review the habit for evergreen perennials. The commencement of a new flush appears to depend on the stimulation of buds by plant growth regulators. The determination of a new flush as vegetative or floral most likely occurs during early shoot development. Cool temperatures are usually florally inductive, but other factors such as periods of water stress before flush commencement, and low crop loads may also enhance induction. There is little evidence for the control of the final size of vegetative shoots, and even less for the size of floral shoots. The time between successive flushes is cyclic and temperature dependent. New work with olive is presented and shows that pruning increases the proportions of vegetative shoots to develop from comparable nodes of pruned and non-pruned branches. It also shows that immature vegetative flushes on olive branches in winter inhibit flowering to some extent, a result previously shown for other evergreen recurrent flushing trees.

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