(234) Aesculus Pollen Viability and Longevity under Frozen Storage

The genus Aesculus (buckeyes and/or horsechestnuts) is composed of 13 species and a number of interspecific hybrids. Pollen from 11 genotypes from five Aesculus species and the hybrid Aesculus ×carnea were used to develop an in-vitro germination test to evaluate pollen viability under various storage treatments. This test was optimized using samples of both fresh pollen and pollen that had been stored up to 1 year. The most effective medium contained 20% sucrose, 100 mg·L-1 H2BO3, 150 mg·L-1 Ca(NO3)2, and 1% agar. The highest germination percentage was observed at 15 °C across all storage treatments. Fresh pollen germinated in excess of 80% over a wide range of germination temperatures. Based on this, all specimens studied would be good pollen parents. The differences in pollen germination between storage at -20 and -80 °C were nonsignificant, but the duration of the storage period was highly significant. At 3 months, viability remained above 60% for four of the six species/hybrid tested. However, at 12 months, all pollen tested dropped below the threshold for good fruit set based on in-vitro pollen germination. Based on these observations, short-term pollen storage may permit crosses between parents with temporally separate flowering phenologies. However, conventional storage procedures are inadequate to maintain pollen collected from a male parent for crosses in subsequent growing seasons.

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Pollen viability in Kunzea pomifera (Myrtaceae) as influenced by sucrose concentration and storage

Australian Journal of Botany ◽

10.1071/bt04054 ◽

2006 ◽

Vol 54 (6) ◽

pp. 553 ◽

Cited By ~ 2

Author(s):

T. Page ◽

G. M. Moore ◽

J. Will ◽

G. M. Halloran

Keyword(s):

Pollen Germination ◽

Storage Time ◽

Sucrose Concentration ◽

Pollen Viability ◽

Germination Percentage ◽

Liquid Media ◽

Controlled Pollination ◽

Fresh Pollen ◽

And Storage

In vitro germination of freshly collected pollen was examined for five genotypes of Kunzea pomifera in liquid media with 5% and 10% sucrose. There were no significant differences in germination level between the different sucrose concentrations for each of the genotypes. Pollen-germination levels were significantly different among genotypes, ranging from 7 to 89%. Pollen of one genotype, stored for 12 months at 4°C and 10% relative humidity, was evaluated for germination at intervals over this period, and while significant differences in the levels of pollen germination after different storage periods were found, there was no correlation between germination percentage and storage time. Pollen of the K. pomifera accession Kmt1 stored under such conditions for 370 days was used in controlled pollination with accession Kmt2, resulting in 3.4 seeds per fruit, which was significantly fewer than the 17.2 seeds per fruit when using fresh pollen.

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Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochthon Apple Cultivars

Frontiers in Plant Science ◽

10.3389/fpls.2021.709231 ◽

2021 ◽

Vol 12 ◽

Author(s):

Dušica Ćalić ◽

Jelena Milojević ◽

Maja Belić ◽

Rade Miletić ◽

Snežana Zdravković-Korać

Keyword(s):

Room Temperature ◽

Storage Temperature ◽

Pollen Viability ◽

Storage Period ◽

Pollen Storage ◽

Apple Cultivars ◽

Pollen Longevity ◽

Fresh Pollen ◽

Storage Temperatures

Globalization has drastically reduced the number of autochthon apple cultivars in the Serbian market and most of them have nearly disappeared; however, some of these cultivars, such as Petrovača, Budimka, Kolačara Pozna, and Kožara, have extraordinary quality, good pomological characteristics, and pest and disease resistance. The present study was conducted to develop a protocol for the storage of pollen for further use in the conservation and breeding of these cultivars. Viability and germination of the mature pollen were tested in vitro, at four storage temperatures (20, 4, −20, and −80°C), right after harvest or 1, 2, 3, 4, 5, and 6 months after storage. Differences in fresh pollen viability and germination between cultivars were statistically significant and ranged from 60 to 88% and 59 to 98%, respectively. Fresh pollen of cv. Budimka showed the highest viability and germination in comparison with other cultivars, especially cv. Kožara. Pollen viability and germination decreased over the storage period, and it was the lowest after 6 months of storage at room temperature in all tested cultivars. Storage at 4°C prolonged the pollen viability and germinability of 1–5 fold, depending on the cultivar and treatment duration; however, the pollen longevity of all cultivars was significantly extended when stored at −20 or −80°C. After 6 months, pollen of cv. Budimka stored at −20 and −80°C showed 14–15 fold higher germination rates in relation to pollen storage at room temperature for the same period. The results of the present study suggest that the pollen of these apple cultivars could be efficiently maintained at −20°C and could be further used for breeding purposes, e.g., for crossings between cultivars that flower at different times of the year.

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Technique for In Vitro Pollen Germination and Short-term Pollen Storage in Caladium

HortScience ◽

10.21273/hortsci.39.2.365 ◽

2004 ◽

Vol 39 (2) ◽

pp. 365-367 ◽

Cited By ~ 7

Author(s):

Zhanao Deng ◽

Brent K. Harbaugh

Keyword(s):

Pollen Germination ◽

Large Scale ◽

Pollen Viability ◽

Storage Conditions ◽

In Vitro Germination ◽

Short Term ◽

Pollen Storage ◽

Breeding Programs ◽

Sucrose Level

The sporadic nature of inflorescence production and flower protogyny in caladium (Caladium ×hortulanum Birdsey) makes it desirable to store pollen and to rapidly assess its viability for cross-pollinations in breeding programs. This study was conducted to develop a procedure to determine caladium pollen viability and to use that procedure to evaluate the effect of short-term storage conditions on pollen viability. The sucrose level in the culture medium was found to have a significant impact on the in vitro germination of caladium pollen; a concentration of 6.8% was determined to be optimal for pollen germination. Caladium pollen lost viability within 1 day under room (24 °C) or freezing (-20 °C) temperatures, but could be stored at 4 °C for 2 to 4 days. Pollen stored at 4 °C produced successful pollinations. Data obtained from large-scale greenhouse pollinations supported use of this in vitro germination assay as a convenient way to evaluate caladium pollen viability (and fertility).

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Low Temperature Storage and in vitro Pollen Germination of Selected Spring Wheat Accessions

Journal of Agricultural Science ◽

10.5539/jas.v9n9p1 ◽

2017 ◽

Vol 9 (9) ◽

pp. 1

Author(s):

Bahram Baninasab ◽

Mirko Tabori ◽

Junjie Yu ◽

Yuxue Zhang ◽

Xuelian Wang ◽

...

Keyword(s):

Spring Wheat ◽

Pollen Germination ◽

Pollen Viability ◽

Triticum Aestivum L ◽

In Vitro Pollen Germination ◽

Low Temperature Storage ◽

Fresh Pollen ◽

Storage Temperatures ◽

Temperature Storage

The study was conducted to evaluate in vitro pollen germination of 50 spring wheat (Triticum aestivum L.) accessions at three storage temperatures (23 °C, 5 °C, and -20 °C). Germination of the mature pollen was measured right after harvest (0 time), and 24, 48, and 72 h after storage (HAS). Differences in fresh pollen germination between accessions were significant and ranged from 21.15% to 1.09%. Pollen germination was the lowest at 24 h at room temperature. The pollens of 24 wheat accessions (AAC Scotia, AW636, AW725, AW780, AW804, AW822, AW823, Brookfield, BRS Pardela, ECSW05, ECSW38, ECSW48, ECSW49, ECSW69, Hoffman, Kleos, Major, Nass, 12NQW-237, 12NQW-413, 12NQW-414, 12NQW-436, 12NQW-754, and 12BW0374) were viable after 24 h when they were stored at 5 °C. Sub-zero temperature did not prolong pollen viability.

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Effect of Pollen Genotype, Temperature and Period of Storage on In Vitro Germinability and In Vivo Seed Set in Chrysanthemum—Preliminary Study

Agronomy ◽

10.3390/agronomy11122395 ◽

2021 ◽

Vol 11 (12) ◽

pp. 2395

Author(s):

Natalia Miler ◽

Anita Wozny

Keyword(s):

Seed Set ◽

Pollen Viability ◽

Chrysanthemum Morifolium ◽

Pollen Storage ◽

Cross Breeding ◽

Pollen Donor ◽

Pollen Germinability ◽

Fresh Pollen

Among many challenges in chrysanthemum cross-breeding, the access to viable pollen for hybridization of cultivars distant in location and different in flowering time is required. Low pollen viability along with incompatibility are mainly responsible for low seed set in modern chrysanthemum cultivars. The aim of the study was to test various temperatures and periods of pollen storage of Chrysanthemum × morifolium in order to elaborate the method of chrysanthemum pollen preservation for cross-breeding purposes. In the first experiment, in vitro pollen germination of four cultivars was investigated following storage at 20 °C, 4 °C, −20 °C, and −80 °C, for one, four, and eight weeks. The second experiment focused on in vivo seed set after one week pollen treatment with 20 °C, 4 °C, −20 °C, and −80 °C (three pollen donor cultivars tested). Pollen in vitro germinability, as well as seed set efficiency, was generally low and cultivar dependent. Independent of the period of storage, stored pollen germinability was lower (5.30–6.63%) than fresh pollen (8.15%). Incubation of pollen in −80 °C significantly increased pollen germinability (9.80%), as well as seed set efficiency in comparison to control (19.28% and 10.21%, respectively) provided the cultivars are compatible. Among cultivars, the highest germinability of pollen was found in ‘Brda’ and ‘Donna’ (8.2% and 8.23%, respectively), while ‘Bydgoszczanka’ showed the lowest germinability (2.97%). There were also pollen genotype dependent effects in in vivo seed set efficiency, which was highest in ’Brda’ (17.57%) and much lower in ‘Jutrzenka’ and ‘Polka’ (1.34% and 0.39%, respectively), which contributed to the incompatibility of crossed cultivars rather than pollen viability.

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Storage and Viability Testing of Protea Pollen

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.121.5.804 ◽

1996 ◽

Vol 121 (5) ◽

pp. 804-809 ◽

Cited By ~ 10

Author(s):

I. David van der Walt ◽

Gail M. Littlejohn

Keyword(s):

Storage Temperature ◽

Pollen Viability ◽

Germination Percentage ◽

Long Term Storage ◽

Fresh Pollen ◽

The Relationship ◽

Term Storage ◽

Storage Temperatures

The influence of storage temperature and humidity on pollen viability was studied in four Protea species. Pollen was stored at a range of temperatures and relative humidities for up to 1 year and tested for ability to germinate in vitro. Pollen of P. repens (L.) L. `Sneyd', P. eximia (Salisb. ex Knight) Fourcade `Fiery Duchess' and P. magnifica Link. clone T 84 07 05 stored at -196 °C and -14 to -18 °C retained a germination percentage as high as that of fresh pollen regardless of humidity. Humidity control became increasingly important at storage temperatures above 0 °C. The study showed that long-term storage of Protea pollen is not feasible at temperatures above 0 °C. The relationship between germinability and fluorochromasia (FCR) was studied during storage of `Sneyd' pollen. The correlations between FCR and germinability were found to be low and nonsignificant. Fifteen-month-old cryopreserved `Sneyd' pollen functioned in fertilization and seed set as effectively as fresh pollen.

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Three-year-old Pecan Pollen Retains Fertility

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.115.3.359 ◽

1990 ◽

Vol 115 (3) ◽

pp. 359-363 ◽

Cited By ~ 4

Author(s):

I.E. Yates ◽

Darrell Sparks

Keyword(s):

Fruit Set ◽

Pollen Viability ◽

Storage Conditions ◽

Reliable Indicator ◽

In Vitro Germination ◽

Carya Illinoensis ◽

Pollen Storage ◽

Fresh Pollen

Stored pollen from pecan [Carya illinoensis (Wangenh.) C. Koch] was analyzed for in vitro germination, fertilization efficiency, final fruit set, and characteristics of mature fruits. We demonstrate pecan pollen can be stored for several years and set fruit. Pollen stored for 1, 2, and 3 years at -80C and 1 year at -196C retained the capacity for fertilization. Pollen stored at -196C was more viable than pollen stored at -80C, with no significant correlation between length of storage at -80C, as judged by fruit abortions during the second drop. Final fruit set was not affected by pollen storage conditions, except for pollen collected in a season of drought. Fruit set is a more reliable indicator of pollen viability than in vitro germination. With two minor exceptions, fruits produced with stored pollen were similar to those developing after pollination with fresh pollen.

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Pollen Germination in Different Genotypes of Gladiolus

Nepal Journal of Science and Technology ◽

10.3126/njst.v11i0.4089 ◽

1970 ◽

Vol 11 ◽

pp. 47-50

Author(s):

Tul Bahadur Poon ◽

TM Rao ◽

C Aswath ◽

PE Rajasekharan ◽

DP Kumar

Keyword(s):

Boric Acid ◽

Magnesium Sulphate ◽

Pollen Germination ◽

Pollen Viability ◽

Calcium Nitrate ◽

Potassium Nitrate ◽

Hybrid Selection ◽

Fresh Pollen ◽

Sterile Pollen ◽

Germinated Pollen

Fresh pollen of 16 promising genotypes of gladiolus was tested for their pollen viability. Modified cellophane method was employed to assess the pollen viability. Pollen germination media consisted of 15% sucrose supplemented with 300 ppm calcium nitrate, 200 ppm magnesium sulphate, 100 ppm potassium nitrate and 100 ppm boric acid. Highly significant variations were observed for percentage of pollen germination, non-germinated pollen and sterile pollen. The highest pollen germination (76.41%) was in genotype Hybrid selection 88-10-22, and did not differ significantly from Gladiolus callianthus (75.41%), Sapna (75.10 %), Hybrid selection 86-32-11, (73.28 %), Kum Kum (69.41%), Poonam (69.22 %), Hybrid selection 87-22-1 (67.87 %), Hybrid selection 87-1-1 (67.61 %), Psittacinus hybrid (64.64%) and Darshan (63.97%). The lowest non- germinated pollen (10.47%) was in genotype Gladiolus callianthus, and insignificantly followed by Hybrid selection 88-10-22 with 18.77 % and Hybrid selection 87-22-1 with 18.95 %. The lowest percentage of sterile pollen was noticed in genotype Sapna (2.82%) followed by Poonam with 4.00 % Hybrid selection 88-10-22 with 4.82% and Hybrid selection 82-11-27 with 5.22%. Key words: Gladiolus; germination; pollen DOI: 10.3126/njst.v11i0.4089Nepal Journal of Science and Technology 11 (2010) 47-50

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Foliar Application of Boron to Almond Trees Affects Pollen Quality

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.125.2.265 ◽

2000 ◽

Vol 125 (2) ◽

pp. 265-270 ◽

Cited By ~ 29

Author(s):

A.M.S. Nyomora ◽

P.H. Brown ◽

K. Pinney ◽

V.S. Polito

Keyword(s):

Pollen Germination ◽

Foliar Application ◽

Culture Media ◽

Pollen Viability ◽

Prunus Dulcis ◽

Pollen Tubes ◽

Tube Growth ◽

Mature Trees

The effect of boron (B) on in vivo and in vitro development of almond [Prunus dulcis (Mill.) D.A. Webb (syn. P. amygdalus Batsch)] pollen and pollen tubes and the resultant effect on fruit set was studied in mature trees. The cultivars Mono (pistil donor) and Butte (pollinizer) in an orchard with low soil B in Fresno, California were sprayed with B at 0, 0.8, 1.7, or 2.5 kg·ha-1 during Fall 1993. Pollen viability as indicated by the fluorescein diacetate method (FDA) was >85% and was not affected by field-applied B, however, in vivo pollen germination and tube growth were enhanced by foliar-applied B. More effect of applied B on in vivo growth appeared as pollen tubes progressed toward the ovary. For in vitro germination, foliar-applied B reduced bursting of tubes, and addition of B to the culture media significantly increased pollen germination and pollen tube growth.

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Effect of high temperature on the reproductive development of chickpea genotypes under controlled environments

Functional Plant Biology ◽

10.1071/fp12033 ◽

2012 ◽

Vol 39 (12) ◽

pp. 1009 ◽

Cited By ~ 72

Author(s):

Viola Devasirvatham ◽

Pooran M. Gaur ◽

Nalini Mallikarjuna ◽

Raju N. Tokachichu ◽

Richard M. Trethowan ◽

...

Keyword(s):

Heat Stress ◽

High Temperature ◽

Pollen Germination ◽

Pollen Viability ◽

Pollen Grains ◽

High Temperature Stress ◽

Pollen Production ◽

Seed Number ◽

Controlled Environments

High temperature during the reproductive stage in chickpea (Cicer arietinum L.) is a major cause of yield loss. The objective of this research was to determine whether that variation can be explained by differences in anther and pollen development under heat stress: the effect of high temperature during the pre- and post-anthesis periods on pollen viability, pollen germination in a medium, pollen germination on the stigma, pollen tube growth and pod set in a heat-tolerant (ICCV 92944) and a heat-sensitive (ICC 5912) genotype was studied. The plants were evaluated under heat stress and non-heat stress conditions in controlled environments. High temperature stress (29/16°C to 40/25°C) was gradually applied at flowering to study pollen viability and stigma receptivity including flower production, pod set and seed number. This was compared with a non-stress treatment (27/16°C). The high temperatures reduced pod set by reducing pollen viability and pollen production per flower. The ICCV 92944 pollen was viable at 35/20°C (41% fertile) and at 40/25°C (13% fertile), whereas ICC 5912 pollen was completely sterile at 35/20°C with no in vitro germination and no germination on the stigma. However, the stigma of ICC 5912 remained receptive at 35/20°C and non-stressed pollen (27/16°C) germinated on it during reciprocal crossing. These data indicate that pollen grains were more sensitive to high temperature than the stigma in chickpea. High temperature also reduced pollen production per flower, % pollen germination, pod set and seed number.

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