scholarly journals Postbloom Thinning Response of ‘Bartlett’ Pears to Abscisic Acid

HortScience ◽  
2017 ◽  
Vol 52 (12) ◽  
pp. 1765-1771 ◽  
Author(s):  
Matthew Arrington ◽  
Mateus S. Pasa ◽  
Todd C. Einhorn
Keyword(s):  
Abscisic Acid ◽  
Management Practice ◽  
Net Photosynthesis ◽  
Fruit Weight ◽  
Dependent Manner ◽  
Commercial Thinning ◽  
The Pacific ◽  
Return Bloom ◽  
The Cost ◽  
Dose Dependent

Postbloom thinning of ‘Bartlett’ pears (Pyrus communis L.) is required to produce fruit of commercially acceptable size. In the Pacific Northwestern United States, low temperatures during early stages of pear fruitlet development often limit the efficacy of commercial thinning compounds. Hand thinning, therefore, remains the standard crop load management practice. Chemical thinning protocols are necessary to reduce the cost and dependence on hand labor. The plant hormone abscisic acid (ABA) was evaluated over multiple years in several ‘Bartlett’ pear orchards. ABA was applied to whole canopies at variable rates (50–500 ppm) when fruit diameter was generally between 10 and 12 mm. In three of four trials, ABA thinned in a dose-dependent manner. The relative degree of thinning for a given dose, however, was inconsistent among trials. Trees treated with ABA had a higher proportion of blank and single-fruited spurs than the control. Net photosynthesis (Pn) of single leaves was reduced 75% to 90% within one day of ABA application but gradually returned to ≈80% of control levels within 7 days and fully recovered by ≈14 days. Slightly greater and longer lasting Pn inhibition occurred with increasing ABA dose. Fruit weight and return bloom generally increased with increasing ABA rate. Fruit quality, when measured, was unaffected by ABA treatments. Inconsistent thinning response with ABA may be attributed to environmental factors, biological factors, or both.

scholarly journals Antitranspirational Efficacy and Longevity of Abscisic Acid and a Synthetic Abscisic Acid Analog in Pansies (Viola ×wittrockiana)

HortScience ◽  
2014 ◽  
Vol 49 (6) ◽  
pp. 779-784 ◽  
Author(s):  
Geoffrey M. Weaver ◽  
Marc W. van Iersel
Keyword(s):  
Abscisic Acid ◽  
Product Quality ◽  
Chlorophyll Content ◽  
Stomatal Closure ◽  
Net Photosynthesis ◽  
Dependent Manner ◽  
Leaf Chlorophyll Content ◽  
Rate Dependent ◽  
Leaf Chlorophyll ◽  
Phytotoxic Effect

Physiological antitranspirants can reduce financial risks to growers by temporarily preventing drought stress, improving product quality, and extending the shelf life of ornamental bedding plants. Exogenous abscisic acid (ABA) is an effective antitranspirant that induces stomatal closure in a rate-dependent manner, reducing transpirational water loss in many species. However, it may also cause chlorosis, which reduces product quality. Synthetic ABA analogs have similar effects on stomatal conductance (gS) but are not known to induce chlorosis. We studied the effects of ABA and its analog 8′ acetylene ABA methyl-ester (PBI 429) on gS and net photosynthesis (Pn) in pansies (Viola ×wittrockiana), compared the efficacy and longevity of each compound, and quantified the resulting chlorosis. Plants were treated with spray solutions of ABA (0 to 2000 mg·L−1) and PBI 429 (0 to 200 mg·L−1) and irrigated daily. Gas exchange and leaf chlorophyll measurements were made twice weekly for 2 weeks. Additional measurements were taken once or twice weekly through 47 days. Abscisic acid reduced leaf chlorophyll content and Pn in a rate-dependent manner for 14 days after application but reduced gS for only 11 days, whereas PBI 429 reduced Pn and gS similarly for 7 days and did not reduce leaf chlorophyll content. Reductions in gS and Pn were greatest on the first day after treatment for both compounds. Our results demonstrate that ABA is more effective than PBI 429 at 100 and 200 mg·L−1, but also causes chlorosis, whereas PBI 429 is an effective antitranspirant without this phytotoxic effect.

scholarly journals Transcriptomic analyses show that 24-epibrassinolide (EBR) promotes cold tolerance in cotton seedlings

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0245070
Author(s):  
Lingling Dou ◽  
Yaru Sun ◽  
Shuye Li ◽  
Changwei Ge ◽  
Qian Shen ◽  
...  
Keyword(s):  
Cold Tolerance ◽  
Cold Water ◽  
Net Photosynthesis ◽  
Dependent Manner ◽  
Rna Seq ◽  
Information Process ◽  
Steroidal Hormones ◽  
Mda Content ◽  
Dose Dependent ◽  
Shed Light

In plants, brassinosteroids (BRs) are a class of steroidal hormones that are involved in numerous physiological responses. However, the function of BRs in cold tolerance in cotton has not been explored. In this study, cotton seedlings were treated with five concentrations (0, 0.05, 0.1, 0.2, 0.5 and 1.0 mg/L) of 24-Epibrassinolide (EBR) at 4°C. We measured the electrolyte leakage, malondialdehyde (MDA) content, proline content, and net photosynthesis rate (Pn) of the seedlings, which showed that EBR treatment increased cold tolerance in cotton in a dose-dependent manner, and that 0.2 mg/L is an optimum concentration for enhancing cold tolerance. The function of EBR in cotton cotyledons was investigated in the control 0 mg/L (Cold+water) and 0.2 mg/L (Cold+EBR) treatments using RNA-Seq. A total of 4,001 differentially expressed genes (DEGs), including 2,591 up-regulated genes and 1,409 down-regulated genes were identified. Gene Ontology (GO) and biochemical pathway enrichment analyses showed that EBR is involved in the genetic information process, secondary metabolism, and also inhibits abscisic acid (ABA) and ethylene (ETH) signal transduction. In this study, physiological experiments showed that EBR can increase cold tolerance in cotton seedlings, and the comprehensive RNA-seq data shed light on the mechanisms through which EBR increases cold tolerance in cotton seedlings.

scholarly journals Genotoxic and Mutagenic Effects of the Antifouling Biocide Chlorothalonil on the Estuarine Fish Micropogonias Furnieri, Desmarest, 1823

2021 ◽  
Author(s):  
Muryllo Santos Castro ◽  
Larissa Cristine Carvalho Penha ◽  
Thamires Alexsandra Torres ◽  
Marianna Basso Jorge ◽  
Luis Fernando Carvalho-Costa ◽  
...  
Keyword(s):  
Sublethal Effects ◽  
Test Organism ◽  
Estuarine Fish ◽  
Dependent Manner ◽  
Maritime Navigation ◽  
Commercially Important ◽  
Micropogonias Furnieri ◽  
The Cost ◽  
Mutagenic Effects ◽  
Dose Dependent

Abstract Chlorothalonil is a widely used fungicide in agriculture, and as biocide in antifouling paints. Although it causes toxic effects on non-target organisms and can bioaccumulate in fish tissues, little is known about its sublethal effects. Thus, we evaluated the genotoxic and mutagenic effects of chlorothalonil in Micropogonias furnieri, an estuarine and commercially important fish with potential as a test organism for ecotoxicology assays. We showed that chlorothalonil exerts genotoxic (DNA damage) and mutagenic (micronuclei and nuclear abnormalities) in a dose-dependent manner (0.35 and 3.5 μg g-1). As the genomic instability may lead to carcinogenesis, our data can assist decision-makers with evidence for banning this compound since any benefit to portuary activities and maritime navigation is outweighed by the cost to aquatic ecosystems and to human health.

Effects of Low Molecular Weight Heparin and Unfragmented Heparin on Induction of Osteoporosis in Rats

1990 ◽  
Vol 63 (03) ◽  
pp. 505-509 ◽  
Author(s):  
Thomas Mätzsch ◽  
David Bergqvist ◽  
Ulla Hedner ◽  
Bo Nilsson ◽  
Per Østergaar
Keyword(s):  
Molecular Weight ◽  
Bone Mineral ◽  
Low Molecular Weight Heparin ◽  
Zinc Content ◽  
Low Molecular Weight ◽  
Dependent Manner ◽  
Bone Mineral Mass ◽  
Bone Ash ◽  
Dose Dependent ◽  
Mineral Mass

SummaryA comparison between the effect of low molecular weight heparin (LMWH) and unfragmented heparin (UH) on induction of osteoporosis was made in 60 rats treated with either UH (2 IU/ g b w), LMWH in 2 doses (2 Xal U/g or 0.4 Xal U/g) or placebo (saline) for 34 days. Studied variables were: bone mineral mass in femora; fragility of humera; zinc and calcium levels in serum and bone ash and albumin in plasma. A significant reduction in bone mineral mass was found in all heparin-treated rats. There was no difference between UH and LMWH in this respect. The effect was dose-dependent in LMWH-treated animals. The zinc contents in bone ash were decreased in all heparin-treated rats as compared with controls. No recognizable pattern was seen in alterations of zinc or calcium in serum. The fragility of the humera, tested as breaking strength did not differ between treatment groups and controls. In conclusion, if dosed according to similar factor Xa inhibitory activities, LMWH induces osteoporosis to the same extent as UH and in a dose-dependent manner. The zinc content in bone ash was decreased after heparin treatment, irrespective of type of heparin given.

Effects of NO-Donors on Thrombus Formation and Microcirculation in Cerebral Vessels of the Rat

1996 ◽  
Vol 76 (01) ◽  
pp. 111-117 ◽  
Author(s):  
Yasuto Sasaki ◽  
Junji Seki ◽  
John C Giddings ◽  
Junichiro Yamamoto
Keyword(s):  
Blood Flow ◽  
Thrombus Formation ◽  
Dependent Manner ◽  
Red Cell ◽  
Cell Velocity ◽  
Red Cell Velocity ◽  
The Mean ◽  
Wall Shear ◽  
Dose Dependent

SummarySodium nitroprusside (SNP) and 3-morpholinosydnonimine (SIN-1), are known to liberate nitric oxide (NO). In this study the effects of SNP and SIN-1 on thrombus formation in rat cerebral arterioles and venules in vivo were assessed using a helium-neon (He-Ne) laser. SNP infused at doses from 10 Μg/kg/h significantly inhibited thrombus formation in a dose dependent manner. This inhibition of thrombus formation was suppressed by methylene blue. SIN-1 at a dose of 100 Μg/kg/h also demonstrated a significant antithrombotic effect. Moreover, treatment with SNP increased vessel diameter in a dose dependent manner and enhanced the mean red cell velocity measured with a fiber-optic laser-Doppler anemometer microscope (FLDAM). Blood flow, calculated from the mean red cell velocity and vessel diameters was increased significantly during infusion. In contrast, mean wall shear rates in the arterioles and venules were not changed by SNP infusion. The results indicated that SNP and SIN-1 possessed potent antithrombotic activities, whilst SNP increased cerebral blood flow without changing wall shear rate. The findings suggest that the NO released by SNP and SIN-1 may be beneficial for the treatment and protection of cerebral infarction

Melatonin actions in the heart; more than a hormone

2018 ◽  
Vol 1 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Darío Acuña-Castroviejo ◽  
Maria T Noguiera-Navarro ◽  
Russel J Reiter ◽  
Germaine Escames
Keyword(s):  
Cell Membranes ◽  
Myocardial Cell ◽  
Rat Tissues ◽  
Dependent Manner ◽  
Broad Distribution ◽  
Multiple Organs ◽  
High Doses ◽  
Extrapineal Melatonin ◽  
Dose Dependent ◽  
Different Doses

Due to the broad distribution of extrapineal melatonin in multiple organs and tissues, we analyzed the presence and subcellular distribution of the indoleamine in the heart of rats. Groups of sham-operated and pinealectomized rats were sacrificed at different times along the day, and the melatonin content in myocardial cell membranes, cytosol, nuclei and mitochondria, were measured. Other groups of control animals were treated with different doses of melatonin to monitor its intracellular distribution. The results show that melatonin levels in the cell membrane, cytosol, nucleus, and mitochondria vary along the day, without showing a circadian rhythm. Pinealectomized animals trend to show higher values than sham-operated rats. Exogenous administration of melatonin yields its accumulation in a dose-dependent manner in all subcellular compartments analyzed, with maximal concentrations found in cell membranes at doses of 200 mg/kg bw melatonin. Interestingly, at dose of 40 mg/kg b.w, maximal concentration of melatonin was reached in the nucleus and mitochondrion. The results confirm previous data in other rat tissues including liver and brain, and support that melatonin is not uniformly distributed in the cell, whereas high doses of melatonin may be required for therapeutic purposes.

Evaluating the Proposed Mechanism by Which Atorvastatin Can Protect Renal Tissue against Contrast Media: An Animal study

2019 ◽  
pp. 75-84
Keyword(s):  
Contrast Media ◽  
Kidney Injury ◽  
Saline Group ◽  
Pleiotropic Effect ◽  
Renal Tissue ◽  
Male Rats ◽  
Dependent Manner ◽  
Group 2 ◽  
Dose Dependent ◽  
Media Group

Contrast- induced nephropathy (CIN) is an elevation of serum creatinine of ≥ 0.5 mg/dL from baseline after two to three days of exposure to contrast substance if there is no other cause for acute kidney injury. Atorvastatin may protect normal kidney physiology from contrast- induced kidney injury by effects unrelated to hypolipidemia termed pleiotropic effect by decline of endothelin production, angiotensin system down regulation, and under expression of endothelial adhesion molecules. This study was conducted to assess the strategy by which atorvastatin can achieve protective effect for kidneys after exposure to contrast media in an animal model. A 40 male rats were distributed randomly into 4 groups; ten rats for each: group (1): given normal saline; group (2): CIN group given iopromide as contrast media; group (3): given atorvastatin (20mg/kg) and iopromide; and group (4): given atorvastatin (40mg/kg) and iopromide. Blood collected by cardiac puncture for detection of serum glutathione, malondialdehyde, matrix metalloproteinase-9, and interleukin-18. The results have shown a significant increase in inflammatory and oxidative stress markers in contrast media group, and significant reduction in these markers in atorvastatin treated groups, in a dose-dependent manner. As conclusion, atorvastatin mechanism for protection against CIN in a dose-dependent manner can mediate by anti-inflammatory and antioxidant effects.

Measuring the cost of environmental compliance for Waikato dairy farmers - a survey approach

2015 ◽  
Vol 77 ◽  
pp. 159-166
Author(s):  
T.O.R. Macdonald ◽  
J.S. Rowarth ◽  
F.G. Scrimgeour
Keyword(s):  
Qualitative Data ◽  
Management Practice ◽  
Dairy Farm ◽  
Environmental Compliance ◽  
Dairy Farmers ◽  
The Past ◽  
Farm Systems ◽  
The Cost ◽  
Farm System ◽  
Survey Approach

The link between dairy farm systems and cost of environmental compliance is not always clear. A survey of Waikato dairy farmers was conducted to establish the real (non-modelled) cost of compliance with environmental regulation in the region. Quantitative and qualitative data were gathered to improve understanding of compliance costs and implementation issues for a range of Waikato farm systems. The average oneoff capital cost of compliance determined through a survey approach was $1.02 per kg milksolids, $1490 per hectare and $403 per cow. Costs experienced by Waikato farmers have exceeded average economic farm surplus for the region in the past 5 years. As regulation increases there are efficiencies to be gained through implementing farm infrastructure and farm management practice to best match farm system intensity. Keywords: Dairy, compliance, farm systems, nitrogen, Waikato

Oxygenation of 18-hydroxycorticosterone as the final reaction for aldosterone biosynthesis

1984 ◽  
Vol 107 (3) ◽  
pp. 395-400 ◽  
Author(s):  
Itaru Kojima ◽  
Etsuro Ogata ◽  
Hiroshi Inano ◽  
Bun-ichi Tamaoki
Keyword(s):  
Carbon Monoxide ◽  
Hydroxysteroid Dehydrogenase ◽  
Dependent Manner ◽  
In Vitro Production ◽  
Mitochondrial Preparation ◽  
Final Reaction ◽  
Vitro Production ◽  
Dose Dependent ◽  
Cytochrome P 450

Abstract. Incubation of 18-hydroxycorticosterone with the sonicated mitochondrial preparation of bovine adrenal glomerulosa tissue leads to the production of aldosterone, as measured by radioimmunoassay. The in vitro production of aldosterone from 18-hydroxycorticosterone requires both molecular oxygen and NADPH, and is inhibited by carbon monoxide. Cytochrome P-450 inhibitors such as metyrapone, SU 8000. SU 10603, SKF 525A, amphenone B and spironolactone decrease the biosynthesis of aldosterone from 18-hydroxycorticosterone. These results support the conclusion that the final reaction in aldosterone synthesis from 18-hydroxycorticosterone is catalyzed by an oxygenase, but not by 18-hydroxysteroid dehydrogenase. By the same preparation, the production of [3H]aldosterone but not [3H]18-hydroxycorticosterone from [1,2-3H ]corticosterone is decreased in a dose-dependent manner by addition of non-radioactive 18-hydroxycorticosterone.

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