scholarly journals Stomatal Function and Cuticular Conductance in Whole Tissue-cultured Apple Shoots

1990 ◽  
Vol 115 (3) ◽  
pp. 468-472 ◽  
Author(s):  
K.A. Shackel ◽  
V. Novello ◽  
E.G. Sutter

The relative contribution of stomatal and cuticular conductance to transpiration from whole tissue-cultured apple shoots of Malus pumila Mill. M.26 was determined with a modified steady state porometer. When shoots were exposed to 90% RH and high boundary layer conductance, large (73%) and, in some eases, rapid (2 to 3 hours) reductions in leaf conductance occurred, indicating functional stomata. Stomatal closure was also observed microscopically. A maximum estimate for the cuticular conductance of these apple leaves was 18 to 40 mmol·m-2·s-1, which is lower than previous estimates and close to the upper limit of naturally occurring leaf cuticular conductances. Hence, both stomatal and cuticular restrictions of water loss appear to be of importance in determining the water balance of tissue-cultured apple loots. The pathway of water transport in relation to water stress of tissue-cultured shoots is also discussed.

1990 ◽  
Vol 216 ◽  
pp. 255-284 ◽  
Author(s):  
C. J. Lee ◽  
H. K. Cheng

Global interaction of the boundary layer separating from an obstacle with resulting open/closed wakes is studied for a thin airfoil in a steady flow. Replacing the Kutta condition of the classical theory is the breakaway criterion of the laminar triple-deck interaction (Sychev 1972; Smith 1977), which, together with the assumption of a uniform wake/eddy pressure, leads to a nonlinear equation system for the breakaway location and wake shape. The solutions depend on a Reynolds numberReand an airfoil thickness ratio or incidence τ and, in the domain$Re^{\frac{1}{16}}\tau = O(1)$considered, the separation locations are found to be far removed from the classical Brillouin–Villat point for the breakaway from a smooth shape. Bifurcations of the steady-state solution are found among examples of symmetrical and asymmetrical flows, allowing open and closed wakes, as well as symmetry breaking in an otherwise symmetrical flow. Accordingly, the influence of thickness and incidence, as well as Reynolds number is critical in the vicinity of branch points and cut-off points where steady-state solutions can/must change branches/types. The study suggests a correspondence of this bifurcation feature with the lift hysteresis and other aerodynamic anomalies observed from wind-tunnel and numerical studies in subcritical and high-subcriticalReflows.


1975 ◽  
Vol 53 (24) ◽  
pp. 3041-3050 ◽  
Author(s):  
C. H. A. Little

In experiments with attached and detached shoots of balsam fir, Abies balsamea L., synthetic (±)abscisic acid (ABA) (1) reduced photosynthesis and transpiration by inducing stomatal closure, (2) inhibited indoleacetic acid (IAA) - induced cambial activity in photosynthesizing and non-photosynthesizing shoots, and (3) inhibited the basipetal movement of [14C]IAA. Neither gibberellic acid nor kinetin counteracted the inhibitory effect of (±)ABA on IAA-induced cambial activity. In addition it was demonstrated that increasing the internal water stress increased the level of endogenous ABA in the phloem–cambial region of bark peelings and decreased the basipetal movement of [14C]IAA through branch sections. On the basis of these findings it is proposed that internal water stress inhibits cambial activity, partly through increasing the level of ABA; the ABA acts to decrease the provision of carbohydrates and auxin that are required for cambial growth.


2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Jalel Mahouachi ◽  
María F. López-Climent ◽  
Aurelio Gómez-Cadenas

The pattern of change in the endogenous levels of several plant hormones and hydroxycinnamic acids in addition to growth and photosynthetic performance was investigated in banana plants (Musa acuminatacv. “Grand Nain”) subjected to various cycles of drought. Water stress was imposed by withholding irrigation for six periods with subsequent rehydration. Data showed an increase in abscisic acid (ABA) and indole-3-acetic acid (IAA) levels, a transient increase in salicylic acid (SA) concentration, and no changes in jasmonic acid (JA) after each period of drought. Moreover, the levels of ferulic (FA) and cinnamic acids (CA) were increased, and plant growth and leaf gas exchange parameters were decreased by drought conditions. Overall, data suggest an involvement of hormones and hydroxycinnamic acids in plant avoidance of tissue dehydration. The increase in IAA concentration might alleviate the senescence of survival leaves and maintained cell elongation, and the accumulation of FA and CA could play a key role as a mechanism of photoprotection through leaf folding, contributing to the effect of ABA on inducing stomatal closure. Data also suggest that the role of SA similarly to JA might be limited to a transient and rapid increase at the onset of the first period of stress.


Author(s):  
Rong Fei ◽  
Yuqing Wang ◽  
Yuanlong Li

AbstractThe existence of supergradient wind in the interior of the boundary layer is a distinct feature of a tropical cyclone (TC). Although the vertical advection is shown to enhance supergradient wind in TC boundary layer (TCBL), how and to what extent the strength and structure of supergradient wind are modulated by vertical advection are not well understood. In this study, both a TCBL model and an axisymmetric full-physics model are used to quantify the contribution of vertical advection process to the strength and vertical structure of supergradient wind in TCBL. Results from the TCBL model show that the removal of vertical advection of radial wind reduces both the strength and height of supergradient wind by slightly more than 50%. The removal of vertical advection of agradient wind reduces the height of the supergradient wind core by ~30% but increases the strength of supergradient wind by ~10%. Results from the full-physics model show that the removal of vertical advection of radial wind or agradient wind reduces both the strength and height of supergradient wind but the removal of that of radial wind produces a more substantial reduction (52%) than the removal of that of agradient wind (35%). However, both the intensification rate and final intensity of the simulated TCs in terms of maximum 10-m wind speed show little differences in experiments with and without the vertical advection of radial or agradient wind, suggesting that supergradient wind contributes little to either the intensification rate or the steady-state intensity of the simulated TC.


2004 ◽  
Vol 16 (3) ◽  
pp. 155-161 ◽  
Author(s):  
Mara de Menezes de Assis Gomes ◽  
Ana Maria Magalhães Andrade Lagôa ◽  
Camilo Lázaro Medina ◽  
Eduardo Caruso Machado ◽  
Marcos Antônio Machado

Thirty-month-old 'Pêra' orange trees grafted on 'Rangpur' lemon trees grown in 100 L pots were submitted to water stress by the suspension of irrigation. CO2 assimilation (A), transpiration (E) and stomatal conductance (g s) values declined from the seventh day of stress, although the leaf water potential at 6:00 a.m. (psipd) and at 2:00 p.m. (psi2) began to decline from the fifth day of water deficiency. The CO2 intercellular concentration (Ci) of water-stressed plants increased from the seventh day, reaching a maximum concentration on the day of most severe stress. The carboxylation efficiency, as revealed by the ratio A/Ci was low on this day and did not show the same values of non-stressed plants even after ten days of rewatering. After five days of rewatering only psi pd and psi2 were similar to control plants while A, E and g s were still different. When psi2 decreases, there was a trend for increasing abscisic acid (ABA) concentration in the leaves. Similarly, stomatal conductance was found to decrease as a function of decreasing psi2. ABA accumulation and stomatal closure occurred when psi2 was lower than -1.0 MPa. Water stress in 'Pera´ orange trees increased abscisic acid content with consequent stomatal closure and decreased psi2 values.


2004 ◽  
Vol 31 (12) ◽  
pp. 1149 ◽  
Author(s):  
István Molnár ◽  
László Gáspár ◽  
Éva Sárvári ◽  
Sándor Dulai ◽  
Borbála Hoffmann ◽  
...  

The physiological and morphological responses to water stress induced by polyethylene glycol (PEG) or by withholding water were investigated in Aegilops biuncialis Vis. genotypes differing in the annual rainfall of their habitat (1050, 550 and 225 mm year–1) and in Triticum aestivum L. wheat genotypes differing in drought tolerance. A decrease in the osmotic pressure of the nutrient solution from –0.027 to –1.8 MPa resulted in significant water loss, a low degree of stomatal closure and a decrease in the intercellular CO2 concentration (Ci) in Aegilops genotypes originating from dry habitats, while in wheat genotypes high osmotic stress increased stomatal closure, resulting in a low level of water loss and high Ci. Nevertheless, under saturating light at normal atmospheric CO2 levels, the rate of CO2 assimilation was higher for the Aegilops accessions, under high osmotic stress, than for the wheat genotypes. Moreover, in the wheat genotypes CO2 assimilation exhibited less or no O2 sensitivity. These physiological responses were manifested in changes in the growth rate and biomass production, since Aegilops (Ae550, Ae225) genotypes retained a higher growth rate (especially in the roots), biomass production and yield formation after drought stress than wheat. These results indicate that Aegilops genotypes, originating from a dry habitat have better drought tolerance than wheat, making them good candidates for improving the drought tolerance of wheat through intergeneric crossing.


1998 ◽  
Vol 368 ◽  
pp. 127-153 ◽  
Author(s):  
J. J. STURMAN ◽  
G. N. IVEY

Horizontal exchange flows driven by spatial variation of buoyancy fluxes through the water surface are found in a variety of geophysical situations. In all examples of such flows the timescale characterizing the variability of the buoyancy fluxes is important and it can vary greatly in magnitude. In this laboratory study we focus on the effects of this unsteadiness of the buoyancy forcing and its influence on the resulting flushing and circulation processes in a cavity. The experiments described all start with destabilizing forcing of the flows, but the buoyancy fluxes are switched to stabilizing forcing at three different times spanning the major timescales characterizing the resulting cavity-scale flows. For destabilizing forcing, these timescales are the flushing time of the region of forcing, and the filling-box timescale, the time for the cavity-scale flow to reach steady state. When the forcing is stabilizing, the major timescale is the time for the fluid in the exchange flow to pass once through the forcing boundary layer. This too is a measure of the time to reach steady state, but it is generally distinct from the filling-box time. When a switch is made from destabilizing to stabilizing buoyancy flux, inertia is important and affects the approach to steady state of the subsequent flow. Velocities of the discharges from the end regions, whether forced in destabilizing or stabilizing ways, scaled as u∼(Bl)1/3 (where B is the forcing buoyancy flux and l is the length of the forcing region) in accordance with Phillips' (1966) results. Discharges with destabilizing and stabilizing forcing were, respectively, Q−∼(Bl)1/3H and Q+∼(Bl)1/3δ (where H is the depth below or above the forcing plate and δ is the boundary layer thickness). Thus Q−/Q+>O(1) provided H>O(δ), as was certainly the case in the experiments reported, demonstrating the overall importance of the flushing processes occurring during periods of cooling or destabilizing forcing.


2020 ◽  
Vol 35 (4) ◽  
pp. 1427-1445
Author(s):  
Ewan Short

AbstractForecasters working for Australia’s Bureau of Meteorology (BoM) produce a 7-day forecast in two key steps: first they choose a model guidance dataset to base the forecast on, and then they use graphical software to manually edit these data. Two types of edits are commonly made to the wind fields that aim to improve how the influences of boundary layer mixing and land–sea-breeze processes are represented in the forecast. In this study the diurnally varying component of the BoM’s official wind forecast is compared with that of station observations and unedited model guidance datasets. Coastal locations across Australia over June, July, and August 2018 are considered, with data aggregated over three spatial scales. The edited forecast produces a lower mean absolute error than model guidance at the coarsest spatial scale (over 50 000 km2), and achieves lower seasonal biases over all spatial scales. However, the edited forecast only reduces errors or biases at particular times and locations, and rarely produces lower errors or biases than all model guidance products simultaneously. To better understand physical reasons for biases in the mean diurnal wind cycles, modified ellipses are fitted to the seasonally averaged diurnal wind temporal hodographs. Biases in the official forecast diurnal cycle vary with location for multiple reasons, including biases in the directions that sea breezes approach coastlines, amplitude biases, and disagreement in the relative contribution of sea-breeze and boundary layer mixing processes to the mean diurnal cycle.


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